• Title/Summary/Keyword: mitochondrial COI gene

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Classification and Phylogenetic Studies of Cephalopods from four countries of South-East Asia (동남아시아 4개국 두족류의 분류 및 계통분류학적 연구)

  • Hwang, Hee Ju;Kang, Se Won;Park, So Young;Chung, Jong Min;Song, Dae Kwon;Park, Hyeongchun;Park, Hong Seog;Han, Yeon Soo;Lee, Jun-Sang;Lee, Yong Seok
    • The Korean Journal of Malacology
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    • v.32 no.1
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    • pp.55-62
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    • 2016
  • In this study, an attempt has been made to analyze the morphology of Cephalopods distributed in Korea and collected samples from South-East Asian countries including Thailand, Indonesia, Vietnam, and China. A phylogenetic analysis was performed using the mitochondrial gene, Cytochrome c oxidase subunit I (COI) to understand the genetic divergences of the species and validate their origins. For achieving the objectives, samples were collected directly from Thailand Hat Yai, Songkhla, Indonesia Medan, Vietnam Ho Chi Minh, and Vung Tau in August 2015 and from China in September 2015. A total of 23 species of Cephalopods were identified falling under three orders, four familyies and nine genus. The species were distributed under Order: Octopoda (1 family, 3 genus, and 9 species), Order: Sepiolioda (1 family, 2 genus, and 8 species), and Order Teuthoidea (2 family, 4 genus, and 6 species). 23 species which is 1 family 3 genus 9 species in Octopoda, 1 family 2 genus 8 species in Sepiolioda, 2 family 4 genus 6 species in Teuthoidea. Phylogenetic analysis using COI gene was conducted for 18 species. For the remaining 5 species sequencing results showed severe variation and hence were not considered further. The COI phylogenetic analysis for the 18 species of Cephalopods were found consistent with the morphological identification. The excluded species will be subjected for a further detailed analysis.

The complete mitochondrial genome of the blue-tailed damselfly Ischnura elegans (Odonata: Coenagrionidae)-a climate-sensitive indicator species in South Korea

  • Seung Hyun Lee;Jeong Sun Park;Jee-Young Pyo;Sung-Soo Kim;Iksoo Kim
    • International Journal of Industrial Entomology and Biomaterials
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    • v.46 no.2
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    • pp.41-54
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    • 2023
  • The blue-tailed damselfly, Ischnura elegans Van der Linden, 1820 (Odonata: Coenagrionidae), is a climate-sensitive indicator species in South Korea. In this study, we sequenced the complete mitochondrial genome (mitogenome) of I. elegans collected from South Korea for subsequent population genetic analysis, particularly to trace population movements in response to climate change. The 15,963 base pair (bp)-long complete mitogenome of I. elegans has typical sets of genes including a major non-coding region (the A+T-rich region), and an arrangement identical to that observed in ancestral insect species. The ATP6, ND3 and ND1 genes have the TTG start codon, which, although rare, is the canonical start codon for animal mitochondrial tRNA. The A/T content was 71.4% in protein-coding genes, 72.1% in tRNAs, 72.9% in the whole genome, 74.7% in srRNA, 75.3% in lrRNA, and 83.8% in the A+T-rich region. The A+T-rich region is unusually long (1,196 bp) and contains two subunits (192 bp and 176-165 bp), each of which is tandemly triplicated and surrounded by non-repeat sequences. Comparison of the sequence divergence among available mitogenomes of I. elegans, including the one from the current study, revealed ND2 as the most variable gene, followed by COII and COI, suggesting that ND2 should be targeted first in subsequent population-level studies. Phylogenetic reconstruction based on all available mitogenome sequences of Coenagrionidae showed a strong sister relationship between I. elegans and I. senegalensis.

Genetic Characterization based on a rDNA Spacer, ITS2 and mtDNA, mtCOI Gene Sequences of Korean Venus Clam, Ruditapes philippinarum

  • Park, Gab-Man;Chung, Ee-Yung;Hur, Sung-Bum
    • Proceedings of the Korean Society of Fisheries Technology Conference
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    • 2000.05a
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    • pp.497-498
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    • 2000
  • The venus clam, Ruditapes philippinarum, is an aquaculture shellfish mainly distributed in an intertidal zone of East Asia including Korea, China and Japan. The morphological variation of this species is great. In fact, two of the most popular markers used in molecular evolution, mitochondrial DNA (mtDNA) and nuclear ribosomal DNA (rDNA), have quite different properties, which could translate into different consequences of mutation, drift, migration and selection on patterns of geographical variation and molecular divergence. (omitted)

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Predicting Hosts through Molecular Analysis of Ichneumonid Guts

  • Kang, Gyu Won;Choi, Jin Kyung;Lee, Jong Wook;Suk, Ho Young
    • Animal Systematics, Evolution and Diversity
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    • v.38 no.4
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    • pp.199-204
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    • 2022
  • Ichneumonidae are well-known parasitoids that attack the larvae or pupae of other insects. This study analyzed whether the abdominal DNA of two ichneumonid wasps, Pimpla disparis and Theronia atalantae gestator, showed the signature of the host species, Ivela auripes. Observations confirmed that these two ichneumonids were the representative parasitoid species growing in the larvae of I. auripes. In addition, sequence analysis showed that the mitochondrial cytochrome oxidase I gene of the host was amplified completely from the DNA extracted from the gut tissues of the ichneumonids. Even after 96 h of adulthood, the host's DNA traces did not disappear and were amplified in many individuals. These results suggest a constructive first step for establishing of a host information bank for ichneumonids in the future.

Four New Records of Monogonont Rotifers (Rotifera: Monogononta) from Korea

  • Yang, Hee-Min;Min, Gi-Sik
    • Animal Systematics, Evolution and Diversity
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    • v.38 no.2
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    • pp.51-58
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    • 2022
  • Four monogonont rotifers, Filinia hofmanni Koste, 1980, Lecane pusilla Harring, 1914, Mikrocodides chlaena (Gosse, 1886), and Proales fallaciosa Wulfert, 1937, were newly recorded in Korea. The genera Mikrocodides Bergendal, 1892 and Proales Gosse, 1886 were recorded for the first time in Korea. Mikrocodides chlaena and Proales fallaciosa were found from soil samples and are both soft-bodied species. Filinia hofmanni has previously been recorded mainly in Europe, and this is its first record in Asia. Lecane pusilla is the 24th lecanid rotifer recorded in Korea, and its morphological characteristics are consistent with previous research of L. pusilla. We have provided the morphological diagnoses of the four Korean specimens in this study, along with the partial sequences of mitochondrial cytochrome c oxidase subunit I(COI) gene of three species.

New report of Diekeana insignis(Gorham, 1892) (Coleoptera: Coccinellidae: Epilachnini) in South Korea

  • Sang Woo Jung;I Chan Shin;Yoon-Ho Kim
    • Journal of Species Research
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    • v.12 no.3
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    • pp.240-243
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    • 2023
  • The genus and species, Diekeana insignis(Gorham), is reported for the first time in Korea. Male adults were collected from Geoje Island and Changwon-si, located in the southern part of South Korea. The species of D. insignis(Gorham) is characterized by the following morphological characteristics: body length about 9.4mm; pronotum with transverse black marking in the middle part; each elytron with seven large black markings; penis long, slightly bent at apical part, truncate at apex; parameres narrow and as long as penis guide; penis guide narrow and pointed at apex. We herein provide habitus photographs, illustrations of morphological characters, male genitalia, and a detailed diagnosis. A partial sequence of the mitochondrial COI gene was obtained and provided mtDNA information for this species.

Population Genetic Structure of the Korean Endemic Species, Iksookimia pacifica (Pisces: Cobitidae) Distributed in Northeast Korea (한국고유종 북방종개(어류강, 미꾸리과)의 집단유전학적 구조)

  • Jang, Sook-Jin;Ko, Myeong-Hun;Kwan, Ye-seul;Won, Yong-Jin
    • Korean Journal of Environment and Ecology
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    • v.31 no.5
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    • pp.461-471
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    • 2017
  • Population genetic studies of 10 groups of Iksookimia pacifica were conducted to investigate the genetic diversity and population genetic structure across its known range in South Korea. Population DNA sequences of one mitochondrial gene (mtCOI) and three nuclear genes (IRBP, EGR2B, RAG1) were examined in samples collected from ten streams that flow into the East Sea. Both mitochondrial and nuclear sequences exhibited significant differentiation among populations except a few cases. The Bayesian analysis of the multi-locus genotypes inferred from the DNA sequences of nuclear genes clustered the individual fish largely into two geographical groups: a northern group (from Baebong stream to Cheonjin stream) and a southern group (Yangyangnamdae stream to Gangneungnamdae stream). Given that the streams flowing into the East Sea are geographically isolated water systems, such separation of genotypes can be interpreted by the geographical separation of common ancestors into north and south that had colonized South Korea. Since the initial geographical separation of the ancestral population by north and south, the ancestral groups seem to have experienced further differentiation into the current genetic clusters through the physical isolation of streams by the East Sea in each region. It is notable that many individuals in the Jasan stream formed a genetic cluster with those of Yangyangnamdae and Gangneungnamdae streams which are distant from each other. In addition, mitochondrial gene showed low genetic differentiation between some neighboring populations and very low level of genetic diversity in several populations. The present population genetic study will provide valuable information for the conservation and management of the Korean endemic fish species, I. paicifica.