Animal Systematics, Evolution and Diversity

The Korean Society of Systematic Zoology (한국동물분류학회)
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Predicting Hosts through Molecular Analysis of Ichneumonid Guts

  • Kang, Gyu Won (Insect Inquiry Education Institute, Daegu National University of Education) ;
  • Choi, Jin Kyung (Department of Science Education, Daegu National University of Education) ;
  • Lee, Jong Wook (Georim Entomological Institute) ;
  • Suk, Ho Young (Department of Life Sciences, Yeungnam University)
  • Received : 2022.08.03
  • Accepted : 2022.10.05
  • Published : 2022.10.31
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Abstract

Ichneumonidae are well-known parasitoids that attack the larvae or pupae of other insects. This study analyzed whether the abdominal DNA of two ichneumonid wasps, Pimpla disparis and Theronia atalantae gestator, showed the signature of the host species, Ivela auripes. Observations confirmed that these two ichneumonids were the representative parasitoid species growing in the larvae of I. auripes. In addition, sequence analysis showed that the mitochondrial cytochrome oxidase I gene of the host was amplified completely from the DNA extracted from the gut tissues of the ichneumonids. Even after 96 h of adulthood, the host's DNA traces did not disappear and were amplified in many individuals. These results suggest a constructive first step for establishing of a host information bank for ichneumonids in the future.

Keywords

Acknowledgement

The authors wish to thank the two anonymous reviewers for their valuable comments and suggestions, which improved the quality of the paper. This research was supported by Basic Science Research Program through the National Research Foundation of Korea funded by the Ministry of Education (NRF-2021R1A6A3A01086474).

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