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http://dx.doi.org/10.5635/ASED.2022.38.4.025

Predicting Hosts through Molecular Analysis of Ichneumonid Guts  

Kang, Gyu Won (Insect Inquiry Education Institute, Daegu National University of Education)
Choi, Jin Kyung (Department of Science Education, Daegu National University of Education)
Lee, Jong Wook (Georim Entomological Institute)
Suk, Ho Young (Department of Life Sciences, Yeungnam University)
Publication Information
Animal Systematics, Evolution and Diversity / v.38, no.4, 2022 , pp. 199-204 More about this Journal
Abstract
Ichneumonidae are well-known parasitoids that attack the larvae or pupae of other insects. This study analyzed whether the abdominal DNA of two ichneumonid wasps, Pimpla disparis and Theronia atalantae gestator, showed the signature of the host species, Ivela auripes. Observations confirmed that these two ichneumonids were the representative parasitoid species growing in the larvae of I. auripes. In addition, sequence analysis showed that the mitochondrial cytochrome oxidase I gene of the host was amplified completely from the DNA extracted from the gut tissues of the ichneumonids. Even after 96 h of adulthood, the host's DNA traces did not disappear and were amplified in many individuals. These results suggest a constructive first step for establishing of a host information bank for ichneumonids in the future.
Keywords
COI; Hymenoptera; Ichneumonidae; Lepidoptera; Lymantriidae; parasitoid wasps;
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  • Reference
1 Gauld ID, 1984. An introduction to the Ichneumonidae of Australia. British Museum(Natural History), London, pp. 1-420.
2 Hebert PDN, Stoeckle MY, Zemlak TS, Francis CM, 2004. Identification of birds through DNA barcodes. PLoS Biology, 2:e312. https://doi.org/10.1371/journal.pbio.0020312   DOI
3 Kodani J, Togashi K, 1992. Leaf expansion and shoot elongation of Cornus controversa Hemsley. Japanese Journal of Ecology, 42:115-123.
4 Pilgrim EM, von Dohlen CD, Pitts JP, 2008. Molecular phylogenetics of Vespoidea indicate paraphyly of the superfamily and novel relationships of its component families and subfamilies. Zoologica Scripta, 37:539-560. https://doi.org/10.1111/j.1463-6409.2008.00340.x   DOI
5 Saunders TE, Ward DF, 2018. Variation in the diversity and richness of parasitoid wasps based on sampling effort. PeerJ, 6:e4642. https://doi.org/10.7717/peerj.4642   DOI
6 Vilhelmsen L, Turrisi GF, 2011. Per arborem ad astra: morphological adaptations to exploiting the woody habitat in the early evolution of Hymenoptera. Arthropod Structure and Development, 40:2-20. https://doi.org/10.1016/j.asd.2010.10.001   DOI
7 Yu DS, Van Achterberg C, Horstmann K, 2016. Taxapad 2016, Ichneumonoidea 2015. Database on flash-drive [Internet]. Dicky Sick Ki Yu, Ottawa, Accessed 1 Jan 2016,
8 Zhao ZH, Liu JH, He DH, Guan XQ, Liu WH, 2013. Species composition and diversity of parasitoids and hyper-parasitoids in different wheat agro-farming systems. Journal of Insect Science, 13:162. https://doi.org/10.1673/031.013.16201   DOI
9 Brodeur J, 2012. Host specificity in biological control: insights from opportunistic pathogens. Evolutionary Applications, 5:470-480. https://doi.org/10.1111/j.1752-4571.2012.00273.x   DOI
10 Forbes AA, Bagley RK, Beer MA, Hippee AC, Widmayer HA, 2018. Quantifying the unquantifiable: why Hymenoptera, not Coleoptera, is the most speciose animal order. BMC Ecology, 18:21. https://doi.org/10.1186/s12898-018-0176-x   DOI
11 Godfray HCJ, 1994. Parasitoids, behavioral and evolutionary ecology. Princeton University Press, Princeton, NJ, pp. 1-473.
12 Cardinal S, Danforth BN, 2011. The antiquity and evolutionary history of social behavior in bees. PLoS ONE, 6:e21086. https://doi.org/10.1371/journal.pone.0021086   DOI
13 Day WH, 1994. Estimating mortality caused by parasites and diseases of insects: comparisons of the dissection and rearing methods. Environmental Entomology, 23:543-550. https://doi.org/10.1093/ee/23.3.543   DOI
14 Funk DJ, Bernays EA, 2001. Geographic variation in host specificity reveals host range evolution in Uroleucon ambrosiae aphids. Ecology, 82:726-739. https://doi.org/10.1890/0012-9658(2001)082[0726:GVIHSR]2.0.CO;2   DOI
15 Hajibabaei M, Janzen DH, Burns JM, Hallwachs W, Hebert PDN, 2006. DNA barcodes distinguish species of tropical Lepidoptera. Proceedings of the National Academy of Sciences of the United States of America, 103:968-971. https://doi.org/10.1073/pnas.0510466103   DOI
16 Hopper KR, Prager SM, Heimpel GE, 2013. Is parasitoid acceptance of different host species dynamic? Functional Ecology, 27:1201-1211. https://doi.org/10.1111/1365-2435.12107   DOI
17 Rougerie R, Smith MA, Fernandez-Triana J, Lopez-Vaamonde C, Ratnasingham S, Hebert PDN, 2011. Molecular analysis of parasitoid linkages (MAPL): gut contents of adult parasitoid wasps reveal larval host. Molecular Ecology, 20:179-186. https://doi.org/10.1111/j.1365-294X.2010.04918.x   DOI
18 Huber JT, 2009. Biodiversity of Hymenoptera. In: Insect biodiversity: science and society (Eds., Foottit RG, Adler PH). Blackwell Publishing, Hoboken, NJ, pp. 303-323. https://doi.org/10.1002/9781444308211.ch12   DOI
19 Konigsmann E, 1978. Das phylogenetische system der Hymenoptera. Teil 3: 'Terebrantes'(Unterordnung Apocrita). Deutsche Entomologische Zeitschrift, 25:1-55. https://doi.org/10.1002/mmnd.19780250102   DOI
20 Lachaud JP, Perez-Lachaud G, 2012. Diversity of species and behavior of hymenopteran parasitoids of ants: a review, Psyche, 2012:134746. https://doi.org/10.1155/2012/134746   DOI
21 Takasuka K, Fritzen NR, Tanaka Y, Matsumoto R, Maeto K, Shaw MR, 2018. The changing use of the ovipositor in host shifts by ichneumonid ectoparasitoids of spiders(Hymenoptera, Ichneumonidae, Pimplinae). Parasite, 25:17. https://doi.org/10.1051/parasite/2018011   DOI
22 Tamura K, Stecher G, Peterson D, Filipski A, Kumar S, 2013. MEGA6: molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution, 30:2725-2729. https://doi.org/10.1093/molbev/mst197   DOI
23 Wyckhuys KAG, Graziosi I, Burra DD, Walter AJ, 2017. Phytoplasma infection of a tropical root crop triggers bottom-up cascades by favoring generalist over specialist herbivores. PLoS ONE, 12:e0182766. https://doi.org/10.1371/journal.pone.0182766   DOI
24 Quicke DLJ, 2015. The Braconid and Ichneumonid parasitoid wasps: biology, systematics, evolution and ecology. John Wiley & Sons, New York, pp. 1-681. https://doi.org/10.1002/9781118907085   DOI
25 Gaston KJ, 1991. The magnitude of global insect species richness. Conservation Biology, 5:283-296. https://doi.org/10.1111/j.1523-1739.1991.tb00140.x   DOI