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Isolation and Characterization of Arrowroot Leaf Proteins (칡잎단백질(蛋白質)의 분리(分離) 및 그 성질(性質)에 관한 연구)

  • Lee, Ka-Sun;Yim, Kook-Yi;Choi, Woo-Young;Oh, Man-Jin
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.14 no.4
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    • pp.345-352
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    • 1985
  • To characterize the protein from arrowroot leaf, proteins were extracted and separated from arrowroot leaf, then its amino acid composition and functional properties were studied. Protein in arrowroot leaf was consisted of 18.5% albumin, 33.5% globulin, 34.0% glutelin, 6.2% protamine and 7.8% insoluble residues. The rates of precipitation of proteins which extracted with water, 1M NaCl, and 0.015N NaOH as a solvent were 84.7% (at pH 3.0), 76.4% (at pH 2.5) and 86.4% (at pH 4.0), respectively. The extracted proteins were separated up to about 90% by organic solvents such as ethanol and acetone at 80% concentration, Composition of arrowroot leaf protein concentrates were: $1{\sim}2%$ moisture, $59{\sim}67%$ protein, $4{\sim}8%$ ash and $5{\sim}6%$ (dialyzed concentrates) or $1{\sim}2%$ (acetone-treated ones) lipid. Main amino acids of the concentrates were aspartic acid, glutamic acid and glycine. Solubility profile of the concentrates according to pH was typical. The minimum solubility (below pH 5.0) of acetone extracted protein concentrates was lower than that of unextracted ones, whereas the reverse was true for pH value above this region. Bulk density, water and fat absorption of the concentrates were attributable to correlation to the treatment of acetone. And the bulk density of the concentrates was negatively correlative to both water and fat absorption. Emulsifying and foaming properties were not varied with the treatment of acetone.

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Mineralogy and Geochemistry of Quaternary Fault Gouges in the Southeastern Korean Peninsula (한반도 동남부 제4기 단층 비지의 광물학적 및 지구화학적 연구)

  • 손승완;장태우;김영규
    • Journal of the Mineralogical Society of Korea
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    • v.15 no.2
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    • pp.85-94
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    • 2002
  • XRF, XRD, EPMA have been used to investigate microstructures and mineralogical changes caused by the faulting and fluids associated with faulting in the Quaternary fault gouge zones at the Sangchon, Ipsil and Wangsan faults located at the southeastern part of the Korean Peninsula. The chemical compositions of faulted rocks and protoliths analyzed by XRF show that the fault gouges are relatively enriched in TiO$_2$, P$_2$O$_{5}$, MgO, and Fe$_2$O$_3$) compared with protoliths, indicating that the fluids associated with faulting were highly activated. XRD results show that the fault gouges predominantly consist of quartz, feldspar, calcite and clay minerals. Clay minerals formed in the gouge zones are mainly composed of smectite characterized by a dioctahedral sheet. Based on EPMA analyses various kinds of sulfide, carbonate, phosphate minerals were identified in the gouge zones and protoliths. Xenotime of grey fault gouge of the Sangchon fault and sulfide minerals of contact andesitic rock of Ipsil fault and contact grey andesitic rock of Wangsan fault were probably formed by inflow of hydrothermal solution associated with faulting prior to the Quaternary. Carbonate minerals of contact andesitic rock and gouge zone of the Ipsil fault were formed by inflow of fluid associated with faulting prior to the Quaternary. They are heavily fractured and have reaction rim on their edge, indicating that faultings and inflow of fluids were highly activated after carbonate minerals were formed. Calcites of Wangsan fault seemed to be formed in syntectonic or posttectonic Quaternary faulting.g.

Development of Adaptive Spatial Filter to Improve Noise Characteristics of PET Images (PET 영상의 잡음개선을 위한 적응적 공간 필터 개발)

  • Woo, S. K.;Choi, Y.;Im, K. C.;Song, T. Y.;Jung, J. H.;Lee, K. H.;Kim, S. E.;Choe, Y. S.;Park, C. C.;Kim, B. T.
    • Journal of Biomedical Engineering Research
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    • v.23 no.3
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    • pp.253-261
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    • 2002
  • A spatially adaptive falter was formulated to imrove PET image qualify and the Performance of the filter was evaluated using simulation and phantom and human PET studies. In the proposed filter. if a pixel was identified as the edge Pixel, the Pixel value was Preserved. Otherwise a Pixel was replaced by the mean of the pixel values weighted by 2:7: 2. A Pixel was identified as the edge Pixel. if it satisfies the following conditions : the number of ADs (absolute difference between center and neighborhood pixels) which is smaller than THl (($pix_max{\times}0.1/log_2(NPM)$, NPM : mean of 6 neighborhood pixels excluding minimum and maximum) is 8-k and the number of ADs which is lager than TH2 ($NPM{\times}0.1$) is k. where k : 2, 3, …, 6. The results of this study demonstrate the superior performance of the Proposed titter compared to Gaussian fitter, weight median filter and subset averaged median filter. The proposed tittering method is simple but effective in increasing uniformity and contrast with minimal degradation of spatial resolution of PET images and thus. is expected to Provide improved diagnositc quality PET images .

A Study of Injection Dose for Patients and Exposure Dose for Technologists from the PET/CT Systems (PET/CT 장비 특성에 따른 방사성 의약품 주입량이 방사선 종사자에게 미치는 영향)

  • Park, Hoon-Hee;Oh, Ki-Beak;Lee, Seung-Jae;Bhan, Young-Kag;Kang, Chun-Goo;Lim, Han-Sang;Kim, Jae-Sam;Lee, Chang-Ho
    • The Korean Journal of Nuclear Medicine Technology
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    • v.15 no.1
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    • pp.45-50
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    • 2011
  • Purpose: It appears the different value when the injection dose is calculating for patients on each PET/CT systems. It directly affects the technologists' radiation exposed dose. We studied the effect of the variable injection doses from several PET/CT systems to exposure dose for technologists. Materials and Methods: Six technologists have worked for 5 months through unit rotations with 3 PET/CT systems {Scanner 1 (S1): 0.15 mCi/kg, Scanner 2 (S2): 0.17 mCi/kg, Scanner 3 (S3): 0.12 mCi/kg}. Eighteen to 19 patients have had examinations per a day on each PET/CT systems. Examination parameters were adjusted to the same. TLDs were used for checking the exposure dose of technologists. Results: Each technologists' the monthly average exposure dose was as follows; S1: 0.76 mSv, S2: 0.93 mSv, S3: 0.47 mSv. The maximum exposure dose was 1.12 mSv, and minimum was 0.42 mSv. The results showed significance in the correlation between the PET/CT system and the exposure dose (p<0.005). Conclusion: When the amount of injection dose was small, the exposure dose was decreased not only the patients but also the technologists. The exposure dose was decreased by the individual proficiency of technologists. However, the low injection dose can highly reduce the exposure dose for technologist so that there will be needed to following studies.

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Guideline in the Management of Antenatally Diagnosed Unilateral Hydronephrosis (산전 초음파로 발견된 수신증의 치료 방침)

  • Hahn Hye-Won;Jun Nu-Lee;Kim Kun-Seok;Moon Dae-Hyuk;Yoon Chong-Hyun;Park Young-Seo
    • Childhood Kidney Diseases
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    • v.7 no.1
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    • pp.60-66
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    • 2003
  • Purpose : Neonatal hydronephrosis has been detected with increasing frequency with the widespread use of prenatal ultrasonography, but the consensus about its postnatal management has not yet been reached, especially about surgical intervention. We attempted to determine the guideline of follow-up study and surgical intervention of hydronephrosis by analyzing clinical outcomes of neonates with hydronephrosis. Materials and Methods : Between 1994 and 2000, 128 hydronephrotic kidneys were postnatally confirmed. Cases associated with other urologic anomalies were excluded and 90 unilateral hydronephrotic kidneys with a minimum follow-up of 12 months were enrolled in this study. We classified the patients into 6 groups according to the anterior posterior pelvic diameter(APPD) at initial ultrasonography(USG) within 1 month after birth. Renal USG and $Tc^{99m}-mercaptoacetyl$ triglycerine(MAG3) scan were done according to a set protocol, and pyeloplasty was performed when indicated according to our protocol. Results : Most cases whose APPD were below 10 mm improved or resolved. Only few cases with APPD above 20 mm showed spontaneous improvement and most(88%) had undergone operation. Those with initial APPD within 10-19 mm showed variable outcomes. When the risk factors for irreversible renal functional deterioration were analyzed, the age at pyeloplasty and pre-operative functional deficit were significant. Conclusion : We concluded that in infants with initial APPD below 10 mm, consideration of surgery is not needed, and in those with initial APPD above 20 mm, early operation is recommended. Our set protocol based on initial USG is useful, but the cut-off value of relative renal function(RRF) for operation might be increased to 40% to improve post operative RRF.

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Identification of New, Old and Mixed Brown Rice using Freshness and an Electronic Eye (신선도와 전자눈을 이용한 현미 신곡, 구곡 및 혼합곡의 판별)

  • Hong, Jee-Hwa;Park, Young-Jun;Kim, Hyun-Tae;Oh, Sang Kyun
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.63 no.2
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    • pp.98-105
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    • 2018
  • The sale of brown rice batches composed of rice produced in different years is prohibited in Korea. Thus, new methods for the identification of the year of production are critical for maintaining the distribution of high quality brown rice. Here, we describe the exploitation of an enzyme that can be used to discriminate between freshly harvested and one-year-old brown rice. The degree of enzyme activity was visualized through freshness test with Guaiacol, Oxydol, and p-phenylenediamine reagents. With electronic eye equipment, we selected 29 color codes for identifying new brown rice and old brown rice. The discrimination power of selected color codes showed a minimum of 0.263 to a maximum of 0.922 and an average value of 0.62. The accuracy with which new brown rice and old brown rice could be identified was 100% in principal component analysis (PCA) and discriminant function analysis (DFA). The DFA analysis had greater discriminatory power than did the PCA analysis. A verification test using new brown rice, old brown rice, or a mixture of the two was then performed to validate our method. The accuracy of identification of new and old brown rice was 100% in both cases, whereas mixed brown rice samples were correctly classified at a rate of 96.9%. Additionally, in order to test whether the discriminant constructed in winter can be applied to samples collected in summer, new and old brown rice stored for 8 months were collected and tested. Both new and old brown rice collected in summer were classified as old brown rice and showed 50% identification accuracy. We were able to attribute these observations to changes in enzyme content over time, and therefore we conclude, it will be necessary to develop discriminants that are specific to distinct storage periods in the near future.

Growth Characteristics, Seed and Forage Productivity of New Naked Oat (Avena nuda L.) Cultivar "Jungmo2005" (귀리 품종 '중모2005'의 생육특성과 종실 및 조사료 생산성)

  • Han, Ouk-Kyu;Park, Tae-Il;Park, Hyung-Ho;Park, Ki-Hun;Oh, Young-Jin;Ku, Ja-Hwan;Kweon, Soon-Jong;Ahn, Jong-Woong
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.37 no.1
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    • pp.28-34
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    • 2017
  • A new naked oat cultivar, 'Jungmo2005'(Avena nuda L.), was developed for food and forage use by National Institute of Crop Science, RDA in 2010. It was derived from a cross between $F_1$['Early80'/'Gwiri33'] and $F_1$['Early80'/'Gwiri23']. 'Early80', a covered oats, has early heading and high yielding, while 'Gwiri23' of covered type and 'Gwiri33' of naked type has early heading with large-size grain. 'Jungmo2005' has the characteristics of narrow and long leaves of pale green color, middle diameter culm of yellow color and medium grain of whitish yellow color. Cultivar 'Jungmo2005' had 2 days earlier heading date (May 9) than the check cultivar 'Seonyang' (May 11) in field condition. The 'Jungmo2005' showed better winter hardiness than that of the check cultivar, and similar to the check cultivar in respect to lodging resistance. The 'Jungmo2005' had 105 cm of culm length, 21.5 cm of spike length, 658 spikes per $m^2$, 82 grains per spike, 23.3 g of 1,000-grain weight, and 622 g of test weight. The grain yield of 'Jungmo2005' was averaged $3.38MT\;ha^{-1}$, which was 6% higher than that of the check. Average forage fresh and dry matter yield of 'Jungmo2005' harvested at milk-ripe stage were 44.8 and $12.6tone\;ha^{-1}$, respectively, compared with 47.5 and $12.5tone\;ha^{-1}$ of the check. The protein content of the 'Jungmo2005' was similar to the check (7.6% and 7.5%, respectively), while ADF (28.6%) and NDF (51.5%) were lower than the check (31.4% and 57.0%, respectively). TDN content and RFV were higher than those of the check (66.3%, 120.3 and 64.1, 105.2, respectively). 'Jungmo2005' is recommended for fall sowing cropping only in the south area where daily minimum mean temperatures are averaged higher than $-4^{\circ}C$ in January, and it should not be cultivated in mountain areas, where frost damages is likely to occur. The areas would do better only to sow in spring season.

Antibacterial activity of new quinolones against Edwardsiella tarda isolated from eel tanks (양만장에서 분리된 Edwardseilla tarda균에 대한 new quinolone계 약물의 항균작용)

  • Choi, Min-Soon;Kim, Kyong-Ho;Seo, Young-Ho;Choi, Sang-Hoon;Park, Kwan-Ha
    • Journal of fish pathology
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    • v.9 no.2
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    • pp.185-193
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    • 1996
  • Extensive acquirement of drug resistance to traditional antibacterial agents poses a serious problem to eel aquaculturists. To collect the basic information for new drug development in the future, we assessed the in vitro antibacterial efficacy of 14 new quinolones with 75 isolates of Edwardsiella tarda from local aquaculture tanks of Anguilla japonica. Of all tested quinolones under development or marketed for human use, DU-6859 was most potent with its $MIC_{50}$ value of $0.05{\mu}g$/ml in broth microdilution assay. The drugs whose $MIC_{50}$ values ranged from 0.2 to $0.78{\mu}g$/ml were T-3762, Bay-y3118, ciprofloxacin, norfloxacin, ofloxcin and tosufloxacin. The weakest group of drugs, with their $MIC_{50}$ being 1.56-$3.13{\mu}g$/ml, were difloxacin, sparfloxacin, fleroxacin, Q-35, amifloxacin, lomefloxacin and enoxacin. The number of resistant strains, when arbitrarily defined with their MICs of $\geq6.25{\mu}g$/ml, was : 3 to T-3762, 3 to Bay-y3118, 44 to difloxacin, 16 to sparfloxacin, 13 to ciprofloxacin, 19 to fleroxacin, 36 to Q-35). 31 to amifloxacin, 5 to norfloxacin, 13 to ofloxacin, 31 to lomefloxacin, 41 to enoxacin, 12 to tosufloxacin and 0% to DU-6859, respectively. This information can be taken into consideration for the future development of fisheries antibacterial quinolones.

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Regeneration Processes of Nutrients in the Polar Front Area of the East Sea III. Distribution Patterns of Water Masses and Nutrients in the Middle-Northern last Sea of Korea in October, 1995 (동해 극전선역의 영양염류 순환 과정 III. 1995년 10월 동해 중부 및 북부 해역의 수괴와 영양염의 분포)

  • CHO Hyun-Jin;MOON Chang-Ho;YANG Han-Seob;KANG Won-Bae;LEE Kwang-Woo
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.30 no.3
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    • pp.393-407
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    • 1997
  • A survey of biological and chemical characteristics in the middle-northern East Sea of Korea was carried out at 28 stations in October, 1995 on board R/V Tam-Yang. On the basis of the vertical profiles of temperature, salinity and dissolved oxygen, water masses in the study area were divided into 5 major groups; (1) Low Saline Surface Water (LSSW), (2) Tsushima Surface Water (TSW), (3) Tsushima Middle Water (TMW), (4) North Korean Cold Water (NKCW), (5) last Sea Porper Water (ESPW). Other 4 mixed water masses were also observed. It is highly possible that the LSSW which occured at depths of $0\~30m$ in the most southern part of the study area is originated from the Yangtze River (Kiang) of China due to very low salinity $(<32.0\%_{\circ})$ relatively high concentration of dissolved silicate and no sources of freshwater input into that area. Oxygen maximum layer in the vertical profile was located near surface at northern cold waters and became deeper at the warm southern area. Oxygen minimum layer af depths $50\~100m$, which is TMW, were found in only southern area. In the vortical profiles of nutrients, the concentrations were very low in the surface layer and increased drammatically near the thermocline. The highest concentration occurred in the ESPW. The relatively low value of Si/P ratio in the ESPW (13.63) compared to other reports in the East Sea was due to continuous increase of P with depth as well as Si. The N : P ratio was about 6.92, showing that nitrogenous nutrient is the limiting factor for phytoplankton growth. The exponential relationship between Si and P, compared to the linear relationship between N and P, indicates that nitrate and phosphate have approximately the same regenerative pattern, but silicate has delayed regenerative pattern.

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Estimating the Carrying Capacity of a Coastal Bay for Oyster Culture -II. The Carrying Capacity of Geoie-Hansan Bay- (굴 양식수역의 환경용량 산정 -II. 거제 · 한산만의 환경용량-)

  • Park Jong Soo;Kim Hyung Chul;Choi Woo Jeung;Lee Won Chan;Kim Dong Myung;Koo Jun Ho;Park Chung Kil
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.35 no.4
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    • pp.408-416
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    • 2002
  • A 3D hydrodynamic-ecological coupled model was applied to estimate carrying capacity in Geoje-Hansan Bay where is one of the most important oyster culturing grounds in Korea. We considered the carrying capacity as the difference between food supply to the oysters and food demand, considering monthly difference of the actual growth. The food supply to the system was determined from the results of the model simulation (tidal exchange and chlorophyll $\alpha$) over the culturing period from September to May of the following year. The food demand was estimated from the food concentration (chlorophyll $\alpha$) multiple the filtration rate of oysters that is considered monthly different growth rate of oysters and food concentration. The values of carrying capacity for the system varied from 6.1 ton/ha (minimum carrying capacity) in february to 14.91 ton/ha (maximum carrying capacity) in April of marketable size oysters (>4 g wet-tissue weight) depending on temporal variations in the food supply. The oyster production calculated from present facilities was 9 ton/ha in wet-tissue weight in Geoje-Hansan Bay. This value corresponded to $60\%$ of maximum carrying capacity of the system. The optimal carrying capacity without negatively affecting on oyster production was 5.5 ton/ha when calculated from annual statistic data and 6.1 ton/ha when determined by this study. These results suggest that it must be reduced $32\%$~$39\%$ of oyster facilities in the system.