• 한국연소학회:학술대회논문집
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• 한국연소학회 2002년도 제24회 KOSCO SYMPOSIUM 논문집
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• pp.123-132
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• 2002

A numerical study was conducted to investigate the combustion phenomena of normal start and unstart processes based on ISL's RAMAC 30 experiments with different diluent amounts and fill pressures in a ram accelerator. The initial projectile launching speed was 1.8 km/s which corresponded to the superdetonative speed of the stoichiometric $H_2/O_2$ mixture diluted with 5 $CO_2$ or 4 $CO_2$. Experiments with same condition except for projectile surface material demonstrated that ignition was successful with an aluminum projectile, but no combustion was observed in case of a steel projectile. In this study, it was found that neither shock nor viscous heating was sufficient to ignite the mixture at a low speed of 1.8 km/s, as was found in the experiments using a steel projectile. However, we could succeed in igniting the mixtures by imposing a minimal amount of additional heat to the combustor section and simulate the normal start and unstart processes found in the experiments with an aluminum projectile. For the numerical simulation of supersonic combustion, multi-species Navier-Stokes equations coupled with a Baldwin-Lomax turbulence model and detailed chemistry reaction equations of $H_2/O_2/CO_2$ suitable for high-pressure gaseous combustion were considered. The governing equations were discretized by a high order accurate upwind scheme and solved in a fully coupled manner with a fully implicit, time accurate integration method. The numerical results matched almost exactly to the experimental results. As a result, it was found that the normal start and unstart processes depended on the strength of gas mixture, development of shock-induced combustion wave stabilized by the first separation bubble, and its size and location.

• 대한환경공학회지
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• 제33권7호
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• pp.481-484
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• 2011

염소 소독시 유기성 클로라민 생성에 대한 대부분의 연구가 모델 화합물질(예, 아미노산)을 사용한 연구결과로, 실제 자연상태에서 발생된 용존유기질소(DON)에 의한 유기성 클로라민 생성에 적용할 수 없다. 본 연구는 지표수에서 추출된 16개 천연유기물질(NOM)을 사용하여 염소 소독시 DON이 유기성 클로라민 생성에 미치는 영향을 조사하였다. 크게 염소 주입량과 반응시간, 그리고 DON 분자량이 유기성 클로라민 생성에 미치는 영향에 대해 살펴보았다. 염소 주입량을 변화시킨 시험결과, 소비된 염소량 1 mg당 0.25 mg이 유기성 클로라민으로 전환되었다. DON은 염소와 반응하여 10분 이내에 빠르게 유기성 클로라민을 생성하였고, 그 후 계속해서 분해(가수분해, 산화)되었다. 반응시간 10분과 24시간의 유기성 클로라민 평균값은 각각 0.78과 $0.16mg-Cl_2/mg-DON$이었다. NOM의 DOC/DON 비율이 낮을수록, 즉 NOM내 DON의 함량비가 높을수록 유기성 클로라민 생성율이 높았다. 10,000 Da 분획시험 결과, DON의 분자량은 유기성 클로라민의 생성에 큰 영향을 미치지 않았다.

• Journal of Microbiology and Biotechnology
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• 제30권7호
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• pp.982-995
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• 2020

A putative multidrug efflux gene, yddA, was cloned from the Escherichia coli K-12 strain. A drug-sensitive strain of E. coli missing the main multidrug efflux pump AcrB was constructed as a host and the yddA gene was knocked out in wild-type (WT) and drug-sensitive E. coliΔacrB to study the yddA function. Sensitivity to different substrates of WT E.coli, E. coliΔyddA, E. coliΔacrB and E. coliΔacrBΔyddA strains was compared with minimal inhibitory concentration (MIC) assays and fluorescence tests. MIC assay and fluorescence test results showed that YddA protein was a multidrug efflux pump that exported multiple substrates. Three inhibitors, ortho-vanadate, carbonyl cyanide m-chlorophenylhydrazone (CCCP), and reserpine, were used in fluorescence tests. Ortho-vanadate and reserpine significantly inhibited the efflux and increased accumulation of ethidium bromide and norfloxacin, while CCCP had no significant effect on YddA-regulated efflux. The results indicated that YddA relies on energy released from ATP hydrolysis to transfer the substrates and YddA is an ABC-type multidrug exporter. Functional study of unknown ATP-binding cassette (ABC) superfamily transporters in the model organism E. coli is conducive to discovering new multidrug resistance-reversal targets and providing references for studying other ABC proteins of unknown function.

• Journal of Microbiology and Biotechnology
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• 제22권9호
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• pp.1288-1295
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• 2012

Exposure to bioaerosols causes various adverse health effects including infectious and respiratory diseases, and hypersensitivity. Controlling exposure to bioaerosols is important for disease control and prevention. In this study, we evaluated the efficacies of various functional filters coated with antimicrobial chemicals in deactivating representative microorganisms on filters or as bioaerosols. Tested functional filters were coated with different chemicals that included (i) Ginkgo and sumac, (ii) Ag-apatite and guanidine phosphate, (iii) $SiO_2$, ZnO, and $Al_2O_3$, and (iv) zeolite. To evaluate the filters, we used a model ventilation system (1) to evaluate the removal efficiency of bacteria (Escherichia coli and Legionella pneumophila), bacterial spores (Bacillus subtilis spore), and viruses (MS2 bacteriophage) on various functional filters, and (2) to characterize the removal efficiency of these bioaerosols. All experiments were performed at a constant temperature of $25^{\circ}C$ and humidity of 50%. Most bacteria (excluding B. subtilis) rapidly decreased on the functional filter. Therefore, we confirmed that functional filters have antimicrobial effects. Additionally, we evaluated the removal efficiency of various bioaerosols by these filters. We used a six-jet collision nebulizer to generate microbial aerosols and introduced it into the environmental chamber. We then measured the removal efficiency of functional filters with and without a medium-efficiency filter. Most bioaerosol concentrations did not significantly decrease by the functional filter only but decreased by a combination of functional and medium-efficiency filter. In conclusion, functional filters could facilitate biological removal of various bioaerosols, but physical removal of these by functional was minimal. Proper use of chemical-coated filter materials could reduce exposure to these agents.

• 한국항해항만학회지
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• 제32권8호
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• pp.589-596
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• 2008

선박의 접안운동을 자동화하기 위하여 인공신경망(Artificial Neural Network, 이하 ANN)에 의한 제어를 수행하였다. ANN은 시스템의 비선형성이 표현 가능하므로 접안운동과 같은 비선형성이 강한 조종운동에 적합하다. 입력층과 출력층 사이에 하나 이상의 중간층이 존재하는 다층 인식자(Multi-layer perceptron)를 사용하였고, 교사 데이터(Teaching data)와 역전파(Back-Propagation) 알고리즘을 사용하여 신경망의 출력값과 목표 출력값 사이의 오차가 최소가 되도록 신경망 학습을 수행하였다. 접안 시 저속조종 수학모델을 사용하여 접안 시뮬레이션을 수행하였으며, ANN의 입력층 성분(unit)이 8개인 구조와 6개인 구조의 접안 제어를 비교하였다. 시뮬레이션 결과, 두 ANN에 의하여 접안 경로 선택에 차이가 나타났으나 접안 조건은 모두 만족하였다.

• Transactions on Electrical and Electronic Materials
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• 제17권2호
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• pp.57-68
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• 2016

In this study, we present the modern hybrid system based power generation for electric vehicle applications. We describe the hybrid structure of modified current source based DC - DC converters used to extract the maximum power from Photovoltaic (PV) and Fuel Cell system. Due to reduced dc-link capacitor requirement and higher reliability, the current source inverters (CSI) better compared to the voltage source based inverter. The novel control strategy includes Distributed Maximum Power Point Tracking (DMPPT) for photovoltaic (PV) and fuel cell power generation system. The proposed DC - DC converters have been analyzed in both buck and boost mode of operation under duty cycle 0.5>d, 0.5<d<1 and 0.5<d for capable electric vehicle applications. The proposed topology benefits include one common DC-AC inverter that interposes the generated power to supply the charge for the sharing of load in a system of hybrid supply with photovoltaic panels and fuel cell PEM. An improved control of Direct Torque and Flux Control (DTFC) based induction motor fed by current source converters for electric vehicle.In order to achieve better performance in terms of speed, power and miles per gallon for the expert, to accepting high regenerative braking current as well as persistent high dynamics driving performance is required. A simulation model for the hybrid power generation system based electric vehicle has been developed by using MATLAB/Simulink. The Direct Torque and Flux Control (DTFC) is planned using Xilinx ISE software tool in addition to a Modelsim 6.3 software tool that is used for simulation purposes. The FPGA based pulse generation is used to control the induction motor for electric vehicle applications. FPGA has been implemented, in order to verify the minimal error between the simulation results of MATLAB/Simulink and experimental results.

• 약학회지
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• 제49권4호
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• pp.323-329
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• 2005

Antimicrobial peptides are evolutionary ancient weapons for animal and plant species to depend themselves against infectious microbes. In the present study, we investigated if an antimicrobial peptide was produced from Coptidis Rhizoma. For the determination, protein substance from the medicinal plant was isolated by various preparations. Among the preparations, the protein portion dissolved in phosphate-buffered saline solution (CRP-DS) that contained the most amount of protein $(90\%)$ resulted in maximal inhibition of Candida albicans which causes local and systemic infections. Analyses by gel-electrophoresis and gel-permeation chromatography showed the CRP-DS formed a single band of approximately 11.8 KDa as molecular size. Antifungal activity of the CRP-DS was almost equivalent to antifungal activity by fluconazole, resulting in MIC (minimal inhibitory concentration) of approximately $50{\mu}g/ml$. The antifungal activity was a dose-dependent. The antifungal activity appeared to be inactivated by heat-treatment and ionic strength, respectively. In a murine model, the CRP-DS enhanced resistance of mice against disseminated candidiasis. The HPLC analysis demonstrated maximum $4\%$ of berberine as residual content in the CRP-DS preparation resulted in no influence on the antifungal activity. In addition, protein portion isolated from Phellodendri Cortex producing the alkaloid component like Coptidis Rhizoma had no such anticandidal effect. These results indicate that the protein substance from Coptidis Rhizoma was responsible for the antifungal activity.

• 한국지하수토양환경학회지:지하수토양환경
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• 제11권4호
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• pp.10-17
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• 2006

본 연구에서는 실험실 규모의 2D air/bio-sparging 장치를 이용하여 공기주입량과 공기주입방식이 디젤오염 토양/지하수의 복원에 미치는 영향을 평가하고 최적 조건을 도출하였다. 최적의 공기주입량과 공기주입방식은 1,000 ml/min의 공기주입과 15 min 간격의 간헐적인 공기주입 패턴이 효율적으로 관찰되었고, 실험기간동안 TPH와 DO 감소, $CO_2$ 생성은 오염원인 디젤이 효과적으로 생분해에 의해 제거가 이루어지고 있음 보여주었다. 즉, 최소한의 공기주입으로도 포화층에서의 디젤 분해를 효과적으로 증진시킬 수 있었으며, 간헐적인 공기주입방식은 대수층내에서의 공기 전달을 증진시킬 수 있었다. 또한 Air/bio-sparging공법의 대부분의 운전비가 blower와 off-gas 처리 시스템에 사용되는 전력비라는 것을 감안 할 때, 최적의 공기주입량과 간헐적인 공기주입방식은 운전비 절감의 효과를 가져다 줄 것으로 판단된다.

• Bulletin of the Korean Chemical Society
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• 제28권12호
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• pp.2377-2381
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• 2007

Solvolyses of 3,4-dimethoxybenzenesulfonyl chloride (DSC) in water, D2O, CH3OD, and in aqueous binary mixtures of acetone, acetonitrile, 1,4-dioxane, ethanol, methanol, and 2,2,2-trifluoroethanol (TFE) have been investigated at 25.0 oC. Kinetic solvent isotope effects (KSIE) in water and in methanol and product selectivities in alcohol-water mixtures are also reported. The Grunwald-Winstein plot of first-order rate constants for the solvolyic reaction of DSC with YCl shows marked dispersions into separated lines for various aqueous mixtures. With use of the extended Grunwald-Winstein equation, the l and m values obtained are 1.12 and 0.58 respectively for the solvolyses of DSC. The relatively large magnitude of l is consistent with substantial nucleophilic solvent assistance. From Grunwald-Winstein plots the rate data are dissected approximately into contributions from two competing reaction channels. This interpretation is supported for alcohol-water mixtures by the trends of product selectivities, which show a maximum for ethanol-water mixtures. From the KSIE of 1.45 in methanol, it is proposed that the reaction channel favored in methanolwater mixtures and in all less polar media is general-base catalysed and/or is possibly (but less likely) an addition-elimination pathway. Also, the KISE value of 1.35 for DSC in water is expected for SN2-SN1 processes, with minimal general base catalysis, and this mechanism is proposed for solvolyses in the most polar media.

• 소성∙가공
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• 제23권8호
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• pp.501-506
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• 2014

Progressive shearing with blanking dies is commonly employed to produce large quantities of tiny sheet metal electronic parts. Sheet metal pins, which are narrow and long, that are sheared with a progressive die set are often twisted. The twist in the sheet metal pins, which usually occurs in the final shearing operation, generally decreases with increasing blank holding force. The blank holding forces in all shearing operations are not the same because of different shearing positions and areas. In the current study, the optimal layout of the springs in a progressive die set to minimize the twist of the sheet metal pin is proposed. In order to find the holding force acting on the tiny narrow blanks produced with the proposed springs during the shearing process, the equivalent area method is used in the structural analysis. The shearing of the sheet-metal pin was simulated to compute the twist angle associated with the blank holding force. The constraint condition satisfying the pre-set blank holding force from the previous shearing operations was imposed. A design of experiments (DOE) was numerically implemented by analyzing the progressive die structure and by simulating the shearing process. From the meta-model created from the experimental results and by using a quadratic response surface method (PQRSM), the optimal layout of the springs was determined. The twist of sheet metal pin associated with the optimal layout of the springs found in the current study was compared with that of an existing progressive die to obtain a minimal amount of twist.