• International Journal of Oral Biology
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• 제33권4호
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• pp.179-186
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• 2008

Several lines of evidence suggest that osteocytes play a critical role in bone remodeling. Both healthy and apoptotic osteocytes can send signals to other bone surface cells such as osteoblasts, osteoclasts, osteoclast precursors, and bone lining cells through canalicular networks. Osteocytes responding to mechanical strain may also send signals to other cells. To determine the role for osteocytes an mechanical strain in bone remodeling, we examined the effects of fluid flow shear stress on osteoclast precursor cell and osteoblast proliferation and recruitment induced by osteocytes. In addition, the effects of fluid flow shear stress on osteocyte M-CSF, RANKL, and OPG mRNA expression were also examined. MLO-Y4 cells were used as an in vitro model for osteocytes, RAW 264.7 cells and MOCP-5 cells as osteoclast precursors, and 2T3 cells as osteoblasts. MLO-Y4 cells conditioned medium (Y4-CM) was collected after 24h culture. For fluid flow experiments, MLO-Y4 cells were exposed to 2h of pulsatile fluid flow (PFF) at 2, 4, 8, $16{\pm}0.6\;dynes/cm^2$ using the Flexcell $Streamer^{TM}$ system. For proliferation assays, MOCP-5, RAW 264.7, and 2T3 cells were cultured with control media or 10-100% Y4 CM. Cells were cultured for 3d, and then cells were counted. RAW 264.7 and 2T3 cell migration was assayed using transwells with control media or 10-100% Y4-CM. M-CSF, RANKL and OPG in MLO-Y4 mRNA expression was determined by semiquantitative RT-PCR. Y4-CM increased osteoclast precursor proliferation and migration, but decreased 2T3 cell proliferation and migration. CM from MLO-Y4 cells exposed to PFF caused decreased RAW 267.4 cell proliferation and migration and 2T3 migration compared to control Y4-CM. However, Y4-CM from cells exposed to PFF had no effect on 2T3 osteoblastic cell proliferation. PFF decreased RNAKL mRNA and increased OPG mRNA in MLO-Y4 cells compared to control(without PFF). PFF had no effect on M-CSF mRNA expression in MLO-Y4 cells. These results suggest that osteocytes can regulate bone remodeling by communication with osteoclast precursors and osteoblasts and that osteocytes can communicate mechanical signals to other cells.

• Journal of Ginseng Research
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• 제40권4호
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• pp.325-333
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• 2016

Background: Ginseng extracts are known to have angiogenic effects. However, to date, only limited information is available on the molecular mechanism underlying the angiogenic effects and the main components of ginseng that exert these effects. Human umbilical-vein endothelial cells (HUVECs) are used as an in vitro model for screening therapeutic agents that promote angiogenesis and wound healing. We recently isolated gintonin, a novel ginseng-derived lysophosphatidic acid (LPA) receptor ligand, from ginseng. LPA plays a key role in angiogenesis and wound healing. Methods: In the present study, we investigated the in vitro effects of gintonin on proliferation, migration, and tube formation of HUVECs, which express endogenous LPA1/3 receptors. Results: Gintonin stimulated proliferation and migration of HUVECs. The LPA1/3 receptor antagonist, Ki16425, short interfering RNA against LPA1 or LPA3 receptor, and the Rho kinase inhibitor, Y-27632, significantly decreased the gintonin-induced proliferation, migration, and tube formation of HUVECs, which indicates the involvement of LPA receptors and Rho kinase activation. Further, gintonin increased the release of vascular endothelial growth factors from HUVECs. The cyclooxygenase-2 inhibitor NS-398, nuclear factor kappa B inhibitor BAY11-7085, and c-Jun N-terminal kinase inhibitor SP600125 blocked the gintonin-induced migration, which shows the involvement of cyclooxygenase-2, nuclear factor kappa B, and c-Jun N-terminal kinase signaling. Conclusion: The gintonin-mediated proliferation, migration, and vascular-endothelial-growth-factor release in HUVECs via LPA-receptor activation may be one of in vitro mechanisms underlying ginsenginduced angiogenic and wound-healing effects.

• Industrial Engineering and Management Systems
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• 제3권2호
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• pp.129-133
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• 2004

Studies on the optimal location of retail store have been made in case of no obstacle(Minagawa etal. 1999). This paper deals with the location problem of retail store considering obstacles (e.g. rivers, railways, highways, etc.) and obstacle-overcoming points (e.g. bridges, railway crossings, zebra crossings, overpasses, etc.). We assume that (1) commercial goods dealt here are typically convenience goods, (2) the population is granted as potential demand, (3) the apparent demand is a function of the maximum migration length and the distance from the store to customers, (4) the scale of a store is same in every place and (5) there is no competitor. First, we construct the basic model of customers' behavior considering obstacles and obstacle-overcoming points. Analyzing the two dimensional model, the arbitrary force attracting customers is represented as a height of a cone where the retail store is located on the center. Second, we formulate the total demand of customers and determine the optimal location that maximizes the total demand. Finally, the properties of the optimal location are investigated by simulation.

• 한국컴퓨터정보학회논문지
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• 제4권2호
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• pp.70-79
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• 1999

이동 데이터베이스 체계에서 거래처리 기법은 정적 조정자에 의한 거래처리와 조정자 이주에 의한 거래처리 기법이 있다. 조정자 이주에 의한 거래처리 기법은 거래서비스 통화채널 변경이 이루어졌을 경우에 새로운 데이터베이스 서버에서 그 거래를 계속 처리해야한다는 조정자 이주 원리를 적용하는 것이고 조정자 정착에 의한 거래처리 기법은 거래서비스 통화채널 변경이 이루어졌을 경우라 할 지라도 그 거래를 시작한 데이터베이스 서버에 의하여 처리해야 한다는 정적 조정자 원리를 적용하는 것이다. 본 논문에서 이동 거래처리 기법들의 공정한 성능 비교연구를 위하여 기법들과 독립적인 성능모델을 개발하였다. 그리고 이동 거래처리 기법의 성능 모델을 자세히 설명하였고 성능평가의 신뢰성을 위하여 얻어진 모의실험 결과를 자세히 설명하였다. 결국, 이동 거래처리 기법들은 데이터베이스 서버에서 거래처리를 위한 메시지 이동을 최소화함으로서 시스템 성능을 향상시킬 수 있다.

• 지역연구
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• 제14권2호
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• pp.65-75
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• 1998

Education is widely recognized one of main sources for growth. This paper attempts to incorporate the general recognition into formal regional growth model. The model structure is largely neoclassical. It produces a single good with the two factors, educated labor and non-educated labor, via a constant return production migrating to the region with the higher real wage. The educated labor in a region is accumulated by two sources, migration and physical education capital, while the non-educated labor is by only migration. The paper shows that regional growth equilibrium is characterized as a saddle point. This indicates the presence of the minimum threshold size that must be overcome before a region may grow. It contrasts sharply with results obtained in regional growth models. The paper suggests that regional growth is determined less by the technical characteristics of regional production function characteristics of regional production function but by the stock combination of educated function but by the stock combination of education labor and non-educated labor. Based on this result, the impact of agglomeration economies on regional growth is explored. It is by phase diagram demonstrated that the presence of agglomeration economies do not always lead a region to growth since there still exists the minimum threshold even in the presence of agglomeration economies.

• 지역연구
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• 제11권1호
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• pp.31-60
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• 1995

The distribution of economic activity over a mutually exclusive and exhaustive categorical industry-region matrix is modeled as a composition of two random components: the probability-like share distribution of jobs and the dynamic evolution of absolute aggregates. The former describes the individual activity location choice by comparing the predicted profitability of the current industry-region pair against that of all other alternatives based on the available information on industry-specific, region specific, or activity specific attributes. The latter describes the time evolution of macro-level aggregates using a dynamic reduced from model. With the seperation of micro choice behavior and macro dynamic aggregate constraint, the usual independence and identicality assumptions become consistent with the activity share distribution, hence multi-regional industrial migration can be represented by a set of probability evolution equations in a conservative Markovian from. We call this a Micro-Macro Composition Approach since the product of the aggregate prediction and the predicted activity share distribution gives the predicted activity distribution gives the predicted activity distribution which explicitly considers the underlying individual choice behavior. The model can be applied to interesting practical problems such as the plant location choice of multinational enterprise, the government industrial ploicy to attract international firms, and the optimal tax-transfer mix to influence activity location choice. We consider the latter as an example.

• E2M - 전기 전자와 첨단 소재
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• 제9권7호
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• pp.719-726
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• 1996

DLTS measurements were performed to study the annealing induced changes of the trap centers in MOV and to shed more light on the stability mechanism of the MOV. Two electron traps, Ec-0.26[eV] and Ec-(O.2-0.3)[eV], were observed in the unannealed samples in large quantities(7-9 X 1014[CM 3]), whereas the three electron traps Ec-0.17 [eV], Ec-0.26[eV] and Ec-(O.2-0.3)[eV] were observed far less in the annealed samples. The minima in the Ec-0.26[eV] trap density, coupled with the presented results that unannealed devices are unstable whereas 600.deg. C annealed devices are most stable, suggests that the instability of the MOV under long term electrical stressing is related to the Ec-0.26[eV] trap. This results support that the ion migration model for the device instability where the Ec-0.26[eV] defects may be the interstitial zinc or the migrating ions. The interstitial zinc originated as a result of the nonstoichiometric nature of ZnO might cause the degradation of the I-V characteristics of the MOV with long term electrical stressing.

• 한국지형학회지
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• 제23권3호
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• pp.93-104
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• 2016

High school textbooks of world geography show geomorphic featuresin arid environments such as sand dune, yardang and ventifact which are largely created by aeolian processes. Desert pavements, ubiquitous armored surfaces composed of a mosaic of clasts in hot and arid regions, are introduced as a major landform which can be attributed to wind erosion. However, they are formed by a variety of processes including deflation, surface runoff, upward clast migration and dust accretion that cause coarse particles concentration at the surface. The deflation by wind leaving a lag of coarse clasts has been solely regarded at home, even though the classical mechanism of deflation has been evaluated as a relatively unimportant process of pavement formation abroad through empiricalstudies. The accretionary model is gaining wider acceptance, thus implying that desert pavements could be formed through deposition of aeolian material. In addition, sheetflood and upward migration of clasts, irrelevant to the aeolian processes, could also create stone pavements. As a consequence, the deflation process in high school textbooks has to be urgently modified into a range of processes including aeolian mantling. By stressing that desert pavements are an exceptional geomorphic feature in deserts where wind is a predominant geomorphic agent, they can be used as a good example to demonstrate that a landform is not monogenetic.

• 방사성폐기물학회지
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• 제21권4호
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• pp.465-479
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• 2023

To ensure the safety of disposal facilities for radioactive waste, it is essential to quantitatively evaluate the performance of the waste disposal facilities by using safety assessment models. This paper addresses the development of the safety assessment model for the underground silo of Wolseong Low-and Immediate-Level Waste (LILW) disposal facility in Korea. As the simulated result, the nuclides diffused from the waste were kept inside the silo without the leakage of those while the integrity of the concrete is maintained. After the degradation of concrete, radionuclides migrate in the same direction as the groundwater flow by mainly advection mechanism. The release of radionuclides has a positive linear relationship with a half-life in the range of medium half-life. Additionally, the solidified waste form delays and reduces the migration of radionuclides through the interaction between the nuclides and the solidified medium. Herein, the phenomenon of this delay was implemented with the mass transfer coefficient of the flux node at numerical modeling. The solidification effects, which are delaying and reducing the leakage of nuclides, were maintained the integrity of the nuclides. This effect was decreased by increasing the half-life and the mass transfer coefficient of radionuclides.

• 한국정밀공학회:학술대회논문집
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• 한국정밀공학회 2004년도 춘계학술대회 논문요약집
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• pp.49-49
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• 2004

고도화로 정밀해진 현대 과학기기에는 여러 가지 전자 팩키징 제품들이 쓰이고 있으나 이 제품들은 여러 가지 파손인자들의 영향 때문에 고유의 수명을 다하지 못하고 고장이 발생하게 된다. 전자부품 실장에 이용되는 솔더 조인트의 열화에 관련되는 열 피로와 이온 마이그레이션(Migration) 현상이 솔더 조인트의 신뢰성에 영향을 미치는 가장 중요한 인자로 알려져 있고 이러한 인자 이외에도 여러 가지 요인들이 복합적으로 작용하여 솔더의 접합부분에 피로파괴를 일으킨다.(중략)