• Title/Summary/Keyword: microsphere

Search Result 221, Processing Time 0.047 seconds

Preparation and Release Behavior of Ipriflavone-Loaded PLGA Microsphere for Tissue Engineered Bone (이프리플라본을 함유한 생분해성 PLGA 미립구의 제조 및 조직공학적 골재생을 위한 영향평가)

  • So, Jung-Won;Jang, Ji-Wook;Kim, Soon-Hee;Kim, Geun-Ah;Choi, Jin-Hee;Rhee, John-M.;Son, Young-Suk;Min, Byoung-Hyun;Khang, Gil-Son
    • Polymer(Korea)
    • /
    • v.33 no.1
    • /
    • pp.26-32
    • /
    • 2009
  • The aim of this research was to prepare microparticulate systems based on poly (lactide-co-glycolide)(PLGA) for the local release of ipriflavone in order to reduce bone loss. We developed the IP loaded PLGA microspheres using relatively simple oil-in-water(O/W) solvent evaporation method. HPLC was used to perform the in vitro release test of IP and morphology of cell attached on the micro-spheres was investigated using SEM. Cytotoxicity was assayed by cell counting kit-8 (CCK-8) test. Osteogenic differential cells were analyzed by ALP activity. Through RT-PCR analysis, we observed osteocalcin, ALP, and Type I collagen mRNA expression. The release of IP in vitro was more prolonged over 42 days and IP/PLGA microspheres showed the improvement on the cell proliferation, ALP activity and RT-PCR comparing with control (only PLGA). This initial research will be used to direct future work involved in developing this composite injectable bone tissue engineering system.

Recycling of useful Materials from Fly Ash of Coal-fired Power Plant (석탄화력발전소에서 발생되는 비회로부터 유용성분의 회수)

  • Kim, Dul-Sun;Han, Gwang Su;Lee, Dong-Keun
    • Clean Technology
    • /
    • v.25 no.3
    • /
    • pp.179-188
    • /
    • 2019
  • Upon the combustion of coal particles in a coal-fired power plant, fly ash (80%) and bottom ash (20%) are unavoidably produced. Most of the ashes are, however, just dumped onto a landfill site. When the landfill site that takes the fly ash and bottom ash is saturated, further operation of the coal-fired power plant might be discontinued unless a new alternative landfill site is prepared. In this study, wet flotation separation system (floating process) was employed in order to recover unburned carbon (UC), ceramic microsphere (CM) and cleaned ash (CA), all of which serving as useful components within fly ash. The average recovered fractions of UC, CM, and CA from fly ash were 92.10, 75.75, and 69.71, respectively, while the recovered fractions of UC were higher than those of CM and CA by 16% and 22%, respectively. The combustible component (CC) within the recovered UC possessed a weight percentage as high as 52.54wt%, whereas the burning heat of UC was estimated to be $4,232kcal\;kg^{-1}$. As more carbon-containing UC is recovered from fly ash, UC is expected to be used successfully as an industrial fuel. Owing to the effects of pH, more efficient chemical separations of CM and CA, rather than UC, were obtained. The average $SiO_2$ contents within the separated CM and CA had a value of 53.55wt% and 78.66wt%, respectively, which is indicative of their plausible future application as industrial materials in many fields.

The Characteristics of Poly(acrylamide)-SiOx Nanoparticles Prepared by Graft-polymerizaton (그라프트 중합에 의해 만들어진 폴리아크릴아마이드-실리카 나노 입자의 특성)

  • Min, Jun Ho;Min, Seong Kee
    • Applied Chemistry for Engineering
    • /
    • v.21 no.1
    • /
    • pp.34-39
    • /
    • 2010
  • Methacryloxypropyltrimethoxysilane (MPTMS) was used for the surface modification of silica nanoparticles in the toluene dispersion system for 8 h (MPSN). Then, methacryloxypropyl-modified silica nanoparticles were successfully prepared by solutioun polymerization in the ethanol solution at $60^{\circ}C$ for 14 h with adding AIBN initiator. The modification of ultra-fine particles (SiOx-PAA nanospheres) was investigated via EA, XPS, FTIR, TGA, SEM and TEM. The mean diameter of the bare silica nanoparticles, MPSN and SiOx-PAA monodisperse nanoparticles was about 25, 30 and 35 nm, respectively.

Simvastatin loaded porous poly(lactide-co-glycolide)(PLGA) microspheres as delivery systems strategies for injuring tissue and invitro study

  • Bao, Trinh-Quang;Kim, Yang-Hee;Lee, Byong-Taek
    • Proceedings of the Materials Research Society of Korea Conference
    • /
    • 2009.11a
    • /
    • pp.38.2-38.2
    • /
    • 2009
  • Regeration of natural tissuesor to create biological substitutes for defective or lost tissues and organs through the use of cells. In addition to cells and their porous, drugs are required to promote tissue regeneration. Therefore, the present studies were prepared using simvastatim loaded porous poly(lactide-co-glycolide) (PLGA) by double emulsion solvent evaporation water-in-oil-in-water technique (W/O/W) as drug delivery system strategies for injuring tissue. The resulting microspheres were evaluated for morphology, particle size, encapsulation efficiency, degradation of PLGA microspheres in vitro drug release and in vitro cell viability. Scanning electronic microscopic (SEM) showed that the porosities of the particles was changed by experimental conditions and cultured cells were attached well on porous microspheres surface. The X-ray diffraction (XRD) and differential scanning calometry (DSC) analysis indicate thatsimvastatim was highly dipersed in the microsphere at amorphousstate.

  • PDF

Evaluation of alginate microspheres prepared by emulsion method for protein delivery system

  • Park, Ji-Hyun;Lee, Chang-Moon;Kim, Gwang-Yun;Rhee, Joon-Haeng;Lee, Ki-Young
    • 한국생물공학회:학술대회논문집
    • /
    • 2005.04a
    • /
    • pp.574-578
    • /
    • 2005
  • The purpose of the study is to research the proper conditions to prepare the calcium-alginate microspheres using a emulsion method. The calcium-alginate microspheres were prepared at distinct concentrations (alginate; 1%, 1.5%, 2% (w/v), calcium chloride; 2%, 4%, 8%, 10%(w/v)). The shape of the microspheres prepared was spherical. With increasing alginate and calcium chloride concentration the mean size of the microspheres decreased gradually. In release test, the amount of ovalbumin released from the calcium-alginate mcirospheres was decreased by the increasing of alginate and calcium chloride concentration. In this study the best result was obtained at a 2% of alginate concentration and 10% of calcium chloride concentration.

  • PDF

Synthesis of Thin Film Type Cu/ZnO Nanostructure Catalysts for Development of Methanol Micro Reforming System (마이크로 개질기 개발을 위한 박막형 Cu/ZnO 나노구조 촉매 합성)

  • Yeo, Chan Hyuk;Kim, Yeon Su;Im, Yeon Ho
    • Transactions of the Korean hydrogen and new energy society
    • /
    • v.24 no.3
    • /
    • pp.193-199
    • /
    • 2013
  • In this work, thin film type Cu/ZnO nanostructure catalysts were fabricated by several synthetic routes in order to maximize the performance of the micro reforming system. For this work, various Cu/ZnO nanostructure catalysts could be synthesized by means of four approaches which are chemical vapor method, wet solution method and their hybrid method. The reforming performance of these as-synthetic catalysts was evaluated as compared to the conventional catalysts. Among the as-synthetic nanostructures, sphere type catalysts with specific surface of $18.6m^2/g$ showed the best performance of hydrogen production rate of 30ml/min at the feed rate of 0.2ml/min. This work will give the first insight on thin film type Cu/ZnO nanostructure catalyst for micro reforming system for hydrogen production of portable electronic systems.

Role of Detached Particles During Initial Filtration Phase (여과초기에서의 탈착된 입자의 거동)

  • Kim, Ja-Kyum;Tobiason, John E.
    • Journal of Korean Society of Water and Wastewater
    • /
    • v.19 no.1
    • /
    • pp.16-24
    • /
    • 2005
  • Mathematical model was developed to verify a sequential particle removal taking place in a granular media gravity filter. Consequential multi-layer filtration cycle model was applied to verify the fraction of filter effluent particles that are filter influent particles that were never removed as well as the fraction of filter effluent particles that were detached after deposition were performed through laboratory experiments. Three sizes of marker particles were injected ahead of the filter column as a pulse in the presence of four sizes of polystyrene particles that were used as a primary source of particles in the raw suspension to investigate particle attachment alone in contrast to net removal from attachment and detachment. Microscopic counting of filter effluent particles was assumed to reflect attachment. Experimental results indicated that particle detachment is significant beginning from the early phase of filtration. For each size of fluorescent microspheres at one filter depth, fluorescent microsphere removal increased with filter runtime to a maximum due to ripening. The detached fraction of effluent particles increased with particle size and filter depth. The presence of detached particles and the increasing fraction of detached particles in deeper bed were confirmed.

Preparation of Monodisperse Melamine-Formaldehyde Microspheres via Dispersed Polycondensation

  • Cheong, In-Woo;Shin, Jin-Sup;Kim, Jung-Hyun;Lee, Seung-Jun
    • Macromolecular Research
    • /
    • v.12 no.2
    • /
    • pp.225-232
    • /
    • 2004
  • We have successfully synthesized relatively monodisperse and cross-linked melamine-formaldehyde (M-F) microspheres by dispersed polycondensation and subsequent pH adjustment with serum replacement cleaning. The average particle sizes (equation omitted): weight-average and (equation omitted) : number-average), the polydispersity index (equation omitted), the number of particles N$\_$p/ and the gel content of the M-F microspheres were observed by varying the pH, the surfactant concentration, and the polymerization temperature. We observed that both the pH and the polymerization temperature were predominant factors in determining (equation omitted) and N$\_$p/, but the effect that the temperature and pH had on the gel content ( > 94% for all samples) was negligible. The exponents of the slopes of plots of N$\_$p/ versus pH and surfactant concentration were -10 and 0.6, respectively. Particle nucleation and growth were achieved within short periods; the incessant coagulation occurred even in the presence of surfactants.

Preparation and Characterization of Alginate-Chitosan Microsphere for Controlled Delivery of Silver Sulfadiazine (설파디아진은의 방출제어를 위한 알지네이트-키토산 미립구의 제조 및 특성)

  • Cho, Ae-Ri
    • Journal of Pharmaceutical Investigation
    • /
    • v.31 no.2
    • /
    • pp.101-106
    • /
    • 2001
  • Alginate-chitosan (anion-cationic polymeric complex) was prepared to control the release rate of silver sulfadiazine (AgSD). Na-alginate (2%) solution containing AgSD was gelled in $CaCl_2$ solution. The gel beads formed were immediately encapsulated with chitosan (CS). The gel matrix and membrane were then reinforced with chondroitin-6-sulfate (Ch6S). Release rate of AgSD from the gel matrix was investigated by placing alginate beads in the sac of cellulose membrane simmered in HEPES-buffer solution. The concentration of AgSD released was analyzed by UV at 264 nm. Incorporation capacity of AgSD in Ca-alginate gel was more than 90%. Alginate-Ch6S-CS could control the release rate of AgSD. The amount of AgSD release was dependent on the AgSD loading dose. Incorporation of tripolyphosphate (polyanionic crosslinker) onto the alginate-Ch6S-CS bead increased the release rate of AgSD. Collagen-coating had no influence on the AgSD release rate. Alginate-Ch6S-CS beads with a sufficiently high AgSD encapsulation were capable of controlling the release of the drug over 10 days. In summary, alginate-Ch6S-CS beads could be used as a sustained delivery for AgSD and provide local targeting with low silver toxicity and patient discomfort.

  • PDF

Effects of Process Parameters on the Powder Characteristics of Uranium Oxide Kernel Prepared by Sol-gel Process (Sol-gel 공정을 이용한 UO2 kernel 제조에서 공정변수가 입자특성에 미치는 영향)

  • Kim, Yeon-Ku;Jeong, Kyung-Chai;Oh, Seung-Chul;Suhr, Dong-Soo;Cho, Moon-Sung
    • Journal of Powder Materials
    • /
    • v.16 no.4
    • /
    • pp.254-261
    • /
    • 2009
  • In this study, we investigated the unit process parameters in spherical $UO_2$ kernel preparation. Nearly perfect spherical $UO_3$ microspheres were obtained from the 0.6M of U-concentration in the broth solution, and the microstructure of the $UO_2$ kernel appeared the good results in the calcining, reducing, and sintering processes. For good sphericity, high density, suitable microstructure, and no-crack final $UO_2$ microspheres, the temperature control range in calcination process was $300{\sim}450^{\circ}C$, and the microstructure, the pore structure, and the density of $UO_2$ kernel could be controlled in this temperature range. Also, the concentration changes of the ageing solution in aging step were not effective factor in the gelation of the liquid droplets, but the temperature change of the ageing solution was very sensitive for the final ADU gel particles.