Browse > Article

Preparation and Release Behavior of Ipriflavone-Loaded PLGA Microsphere for Tissue Engineered Bone  

So, Jung-Won (Polymer BIN Fusion Research Center, Chonbuk National University)
Jang, Ji-Wook (Polymer BIN Fusion Research Center, Chonbuk National University)
Kim, Soon-Hee (Polymer BIN Fusion Research Center, Chonbuk National University)
Kim, Geun-Ah (Polymer BIN Fusion Research Center, Chonbuk National University)
Choi, Jin-Hee (Polymer BIN Fusion Research Center, Chonbuk National University)
Rhee, John-M. (Polymer BIN Fusion Research Center, Chonbuk National University)
Son, Young-Suk (Musculoskeletal Bioorgan Center, Kyunghee University)
Min, Byoung-Hyun (Cell Theraphy Center, School of Medicine, Ajou University)
Khang, Gil-Son (Polymer BIN Fusion Research Center, Chonbuk National University)
Publication Information
Polymer(Korea) / v.33, no.1, 2009 , pp. 26-32 More about this Journal
Abstract
The aim of this research was to prepare microparticulate systems based on poly (lactide-co-glycolide)(PLGA) for the local release of ipriflavone in order to reduce bone loss. We developed the IP loaded PLGA microspheres using relatively simple oil-in-water(O/W) solvent evaporation method. HPLC was used to perform the in vitro release test of IP and morphology of cell attached on the micro-spheres was investigated using SEM. Cytotoxicity was assayed by cell counting kit-8 (CCK-8) test. Osteogenic differential cells were analyzed by ALP activity. Through RT-PCR analysis, we observed osteocalcin, ALP, and Type I collagen mRNA expression. The release of IP in vitro was more prolonged over 42 days and IP/PLGA microspheres showed the improvement on the cell proliferation, ALP activity and RT-PCR comparing with control (only PLGA). This initial research will be used to direct future work involved in developing this composite injectable bone tissue engineering system.
Keywords
ipriflavone; PLGA; microsphere; osteogenesis; BMSCs;
Citations & Related Records
Times Cited By KSCI : 5  (Citation Analysis)
Times Cited By Web Of Science : 0  (Related Records In Web of Science)
Times Cited By SCOPUS : 0
연도 인용수 순위
1 P. Bianco, M. Riminucci, S. Gronthos, and P. G. Robey, Stem Cells, 19, 180 (2001)   DOI   ScienceOn
2 P. Biano and P. G. Robey, J. Clin. Invest., 105, 1663 (2000)   DOI   ScienceOn
3 Q. Shang, Z. Wang, W. Liu, Y. Shi, L. Cui, and Y. Cao, J. Craniofac. Surg., 12, 586 (2001)   DOI   ScienceOn
4 J. W. So, S. H. Kim, M. O. Baek, J. Y. Lim, B. H. Min, M. S. Kim, H. B. Lee, and G. Khang, Tissue Eng. Regen. Med., 4, 577 (2007)   DOI   ScienceOn
5 R. Q. Brown, A. Mount, and K. J. L. Burg, J. Biomed. Mater. Res., 4, 32 (2005)
6 C. Perka, U. Arnold, R. Spitzer, and K. Lindenhayn, Tissue Eng., 7, 359 (2001)   DOI   ScienceOn
7 E. K. Park, S. Y. Lee, T. H. Kim, P. H. Hoon, G. Khang, Y. S. Son, S. Y. Kim, and S. Y. Kim, Tissue Eng. Regen. Med., 2, 55 (2005)
8 P. Perugini, I. Genta, B. Conti, T. Modena, D. Cocchi, D. Zaffe, and F. Pavanetto, Int. J. Pharm., 256, 153 (2003)   DOI   PUBMED   ScienceOn
9 E. J. Kim, C. S. Park, M. S. Kim, S. H. Cho, J. M. Rhee, H. B. Lee, and G. Khang, Polymer(Korea), 28, 218 (2004)
10 K. S. Park, C. M. Jin, S. J. Yun, K. D. Hong, S. H. Kim, M. S. Kim, J. M. Rhee, and G. Khang, Polymer(Korea), 29, 501 (2005)   과학기술학회마을
11 J. W. Jang, B. Lee, C. W. Han, I. W. Lee, H. B. Lee, and G. Khang, Polymer(Korea), 27, 226 (2003)   과학기술학회마을
12 J. S. Park, J. C. Yang, S. H. Yuk, H. S. Shin, J. M. Rhee, M. S. Kim, H. B. Lee, and G. Khang, Polymer(Korea), 31, 189 (2007)
13 J. Y. Wang, A. E. Baer, V. B. Kraus, and L. A. Setton, Spine, 26, 1747 (2001)   DOI   ScienceOn
14 J. W. So, S. H. Kim, M. O. Baek, J. Y. Lim, H. W. Roh, N. R. Lee, G. H. Ryu, Y. H. Cho, S. J. Lee, and G. Khang, Tissue Eng. Regen. Med., 5, 84 (2008)
15 A. V. Schwartz, J. L. Kelsey, S. Maggi, M. Tuttleman, S. C. Ho, P. V. Jonsson, G. Poor, J. A. Sisson de castro, L. Xu, C. C. Matkin, L. M. Nelson, and S. P. Heyse, Osteoprosis Int., 9, 242 (1999)   DOI   ScienceOn
16 J. S. Lee, J. H. Shin, J. K. Jeong, J. M. Rhee, H. B. Lee, and G. Khang, Polymer(Korea), 27, 9 (2003)   과학기술학회마을
17 H. Shin, J. S. Temenoff, G. C. Browden, K. Zygourakis, M. C. Farach-carson, M. J. Yaszemski, and A. G. Mikos, Biomaterials, 26, 3645 (2005)   DOI   ScienceOn
18 Y. Hong, C. Gao, Y. Xie, Y. Gong, and J. Shen, Biomaterials, 26, 6305 (2005)   DOI   ScienceOn
19 R. G. Payne, J. S. McGonigle, M. J. Yaszemski, A. W. Yasko, and A. G. Mikos, Biomaterials, 23, 4359 (2001)   DOI   ScienceOn
20 G. Khang, M. S. Kim, S. H. Cho, I. W. Lee, J. M. Rhee, and H. B. Lee, Tissue Eng. Regen. Med., 1, 9 (2004)
21 Y. Choi, S. Y. Kim, S. H. Kim, K. S. Lee, C. Kim, and Y. Byun, Int. J. Pharm., 215, 67 (2001)   DOI   PUBMED   ScienceOn
22 S. H. Kim, M. O. Baek, J. W. So, H. W. Roh, N. R. Lee, M. S. Kim, G. H. Ryu, Y. H. Cho, S. J. Lee, G. Khang, and H. B. Lee, Tissue Eng. Regen. Med., 4, 595 (2007)
23 T. L. Arinzeh, S. J. Peter, M. P. Archambault, Chistian van den Bos, S. Gordon, K. Kraus, A. Smith, and S. Kadiyala, J. Bone. Joint. Sug., 85, 1927 (2003)   DOI   ScienceOn
24 S. K. Kim, K. D. Hong, S. H. Kim, M. S. Kim, G. Khang, I. W. Lee, C. W. Han, H. K. Lee, and H. B. Lee, Tissue Eng. Regen. Med., 2, 130 (2005)
25 L. Cui, B. Liu, G. Liu, W. Zhang, L. Cen, J. Sun, S. Yin, W. Liu, and Y. Cao, Biomaterial, 28, 5477 (2007)   DOI   ScienceOn
26 P. D. Ross, Arch. Intern. Med., 156, 1399 (1996)   DOI   PUBMED   ScienceOn
27 S. Michalet, P. Favreau, and R. Stocklin, Cli. Chem. Lab. Med., 41, 1589 (2003)   DOI   ScienceOn
28 L. M. Boyden, J. Mao, J. Belsky, L. Mitzner, A. Farhi, M. A. Mitnick, D. Wu, K. Insogna, and R. P. Lifton, N. Engl. J. Med., 346, 1513 (2002)   DOI   ScienceOn
29 J. C. Cho, G. Khang, H. S. Choi, J. M. Rhee, and H. B. Lee, Polymer(Korea), 24, 728 (2000)   과학기술학회마을