• Title/Summary/Keyword: microscopes

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Taxonomic Implication of the Stellate Trichomes to Genus Deutzia Subsection Coreanae in Korea (한국산 말발도리속, 매화말발도리아절 식물에 대한 성모형태의 분류학적 적용)

  • 정영호
    • Journal of Plant Biology
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    • v.30 no.1
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    • pp.31-41
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    • 1987
  • To reexamine the taxonomic characters of Deutzia coreana, D. tozawae, and D. triradiata, which were belonged to Subsection Coreanae of genus deutzia in Korea, the stellate trichome in the leaf, one of the major diagnostic characters, was investigated with the stereo and scanning electron microscopes. The number of trichome was nearly equal in both sides of the leaf, while the ray was mainly 4 and 5 in D. coreana. However, there was little trichomes in the upper surface of D. tozawae, and in the lower surface of D. triradiata. D. tozawae had 3, 4-rayed trichomes in leaf surfaces, and D. triradiata 3-rayed ones, respectively. As a result, these three species were also distinguishable by the stellate trichomes in the leaf.

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A Study on the Effected Factor for Vibration Criteria of Sensitive Equipment (정밀장비의 진동허용규제치에 미치는 인자에 관한 연구)

  • 이홍기;장강석;김두훈;김사수
    • Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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    • 1998.04a
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    • pp.302-307
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    • 1998
  • In the production of semiconductor wafer, optical and electron microscopes, ion-beam, laser device must maintain their alignments within a sub-micrometer. This equipment requires a vibration free environment to provide its proper function. Especially, lithography and inspection devices, which have sub-nanometer class high accuracy and resolution, have come to necessity for producing more improved giga and tera class semiconductor wafers. This high technology equipments require very strict environmental vibration standard, vibration criteria, in proportion to the accuracy of the manufacturing, inspecting devices. The vibration criteria of high sensitive equipment should be represented in the form of exactness and accuracy, because this is used as basic data for the design of building structure and structural dynamics of equipment. The study on the evaluation of the factors affecting the permissible vibration criteria is required to design the efficient isolation system of the semiconductor manufacturing of equipment. This paper deals with the properties of the effected factor for vibration criteria of high sensitive equipment.

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Morphological Variation of Lingulodinium polyedrum (Dinophyceae) in Culture Specimens and Reinterpretation of the Thecal Formula

  • Kim, Keun-Yong;Yoshida, Makoto;Kim, Chang-Hoon
    • ALGAE
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    • v.20 no.4
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    • pp.299-304
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    • 2005
  • Morphological observation of a unialgal culture of Lingulodinium polyedrum was conducted under light- and scanning electron microscopes. Lingulodinium polyedrum was redefined as having the thecal formula of APC, Q, 5', 6", 6c, 6s, 5'", 2"" using the reinterpreted tabulation system. In our tabulation system, the Q, which is equivalent to the 3' in the strict Kofoidean system, was treated as an auxiliary platelet because of its considerable morphological variations in culture specimens. Subsequently, apical plates were redefined as plates directly connected to the APC and/or the Q. The 1"' and the 1p in the conventional Kofoidean system were treated as a component of the sulcus (the Ssa) and as an antapical plate (the 1""), respectively. Our reinterpreted tabulation system clearly differentiated extant gonyaulacalean taxa.

Ultrastructure of epididymal spermatozoa in three Korean shrews (한국산 땃쥐류 3종에 대한 부정소 미부 정자의 미세구조)

  • 윤명희;정순정
    • Journal of Life Science
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    • v.11 no.1
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    • pp.54-61
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    • 2001
  • The comparative morphology of epididymal spermatozoa in the lesser white-toothed shrew, Crocidura suaveolens, the Japanese white-toothed shrew, C. dsinezumi and the big white-toothed shrew, C. lasiura, belonging to the subfamily Crocidurinae was studied with the light and electron microscopes. The spermatozoa of C. lasiura and C. dsinezumi were characterized by the large acrosome, serrated inner acrosomal membrane, common apical body and fistulous proximal centriole with slightly dense electron granular materials, which are the charateristics of the Crocidurinae. The C. suaveolens, however, is distinguished from the two species mentioned above in the sperm morphology. That is, the spermatozoa possess not only the charateristic of the Crocidurinae such aw the large acrosome, but also those of the Soricinae, i.e. the smooth inner acrosomal membrane, wavy, finger-like and electron-dense apical body, and the solid proximal centriole filled with electron-dense materials. The results suggest that C. suaveolens has conserved characteristics of the Soricinae.

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Use of Modern Microscopes in Analysing Fiber and Paper Properties (II)-New Aspect in Fibrillation of Pulp Fibers during Refining-

  • Kim, Chul-Hwan;Keigh R. Wadhams
    • Journal of Korea Technical Association of The Pulp and Paper Industry
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    • v.31 no.3
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    • pp.60-67
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    • 1999
  • The CLSM and the image analysis technique enhanced observation of fiber wall fibrillation occurred in both the outer and the fiber wall surfaces during refining by non-destructive techniques. In the early stages of refining, it was well observed that a partial separation between the S1 and S2 layer in the secondary wall was made generating a space in the wet fiber walls . With further refining, it was clearly shown that the shear forces imparted by the refiner bar surfaces caused the S1 layer to become totally separated from the S2 layer as well as creating microfibrils. Furthermore, the fibrillation in the inner fiber wall surfaces could be due to the normal force (Fn) by refiner bars, friction force between a fiber and refiner bars (Fs) and inner friction force between fiber walls(fs). It was confirmed that the concept of fibrillation should be extended to fibrillation in the inner fiber wall surfaces as well as internal and external fribrillation.

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Establishment of an Axenic Culture of Microcystin-Producing Microcystis aeruginosa Isolated from a Korean Reservoir

  • Han, Ah-Won;Oh, Kyoung-Hee;Jheong, Weon-Hwa;Cho, Young-Cheol
    • Journal of Microbiology and Biotechnology
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    • v.20 no.7
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    • pp.1152-1155
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    • 2010
  • In order to establish an axenic (bacteria-free) culture of Microcystis aeruginosa NIER 10039 isolated from a Korean reservoir, the culture was subjected to sequential treatment, including ultrasonication, washing, and addition of antibiotics. Three broad-spectrum antibiotics, namely, kanamycin, ampicillin, and imipenem, were applied separately in that order. Axenicity of the culture was confirmed by cultivation on bacterial media and observation under epifluorescence and scanning electron microscopes. We are the first to establish an axenic culture of a Microcystis strain isolated from Korean reservoirs and can be used in physiological and molecular studies to control toxic Microcystis blooms.

Modeling and Control of a Four Mount Active Micro-vibration Isolation System

  • Banik, Rahul;Gweon, Dae-Gab;Hong, Dong-Pyo
    • Proceedings of the Korean Society Of Semiconductor Equipment Technology
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    • 2006.10a
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    • pp.150-153
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    • 2006
  • Micro vibration isolation, typically originated from ground, is always a prime concern for the nano-measurement instruments such as Atomic Force Microscopes. A four mount active vibration isolation system is proposed in this paper. Modeling and control of such a four mount system as analyzed. Combined active-passive isolation principle is used for vibration isolation by mounting the instrument on a passively damped isolation system made of Elastomer along with the active stage in parallel that consists of very soft actuation system, the Voice Coil Motor. The active stage works in combination with the passive stage for working as a very low frequency vibration attenuator.

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Design and Fabrication of a Multi-modal Confocal Endo-Microscope for Biomedical Imaging

  • Kim, Young-Duk;Ahn, Myoung-Ki;Gweon, Dae-Gab
    • Journal of the Optical Society of Korea
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    • v.15 no.3
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    • pp.300-304
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    • 2011
  • Optical microscopes are widely used for medical imaging these days, but biopsy is a lengthy process that causes many problems during the ex-vivo imaging procedure. The endo-microscope has been studied to increase accessibility to the human body and to get in-vivo images to use for medical diagnosis. This research proposes a multi-modal confocal endo-microscope for bio-medical imaging. We introduce the design process for a small endoscopic probe and a coupling mechanism for the probe to make the multi-modal confocal endo-microscope. The endoscopic probe was designed to decrease chromatic and spherical aberrations, which deteriorate the images obtained with the conventional GRIN lens. Fluorescence and reflectance images of various samples were obtained with the proposed endo-microscope. We evaluated the performance of the proposed endo-microscope by analyzing the acquired images, and demonstrate the possibilities of in-vivo medical imaging for early diagnosis.

Ultrastructure of Secretory Duct Development in the Stem of Ginseng (Panax ginseng C.A.Meyer) Seedlings (인삼 유식물체 줄기의 분비관 형성에 관한 미세구조)

  • 류성철
    • Journal of Plant Biology
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    • v.32 no.3
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    • pp.151-162
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    • 1989
  • Secretory ducts in the stem of Panax ginseng seedlings are observed with light and electron microscopes to clarify development of the epithelial cells of secretory ducts. Secretory duct initial cell is developed from procambial cell which originated from initial cell is differentiated into ipithelial cell ofsecretory ducts. Intercellular space between the epithelial cells are gradually expanded and differentiated into duct lumen. Disintegrations of epithelial cells occur throughout all the stages of development. The cytoplasm of epithelial cells darken and the epithelial cell wall are lysed, preceding their disintegraton. In the epithelial cell organelles are scattered in the cytoplasm. Development of vcuoles are sparse at the early stage. Starch grains decreased gradually, while lipid droplets increased. Free ribosomes are distributed throughout the cytoplasm and secretory vesicles which originated from rough endoplasmic reticulum and Golgi complex are fused with the plasmalemma. These suggest that the cellular metabolism is active. Microtubules and plasmodesmata are typically observed in the thickened epithelial cell wall. Secretions are accumulated in duct lumen.

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A Development of Electron Optics System of Mini-Sized SEM (소형주사전자현미경용 전자공학계의 개발)

  • Park, Man-Jin;Kim, Il-Hae;Kim, Dong-Hwan;Jang, Dong-Young;Han, Dong-Chul
    • Transactions of the Korean Society of Machine Tool Engineers
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    • v.16 no.5
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    • pp.140-144
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    • 2007
  • As an electron scanning microscopes has traditionally required a considerably large room equipped with several service and pipe lines due to its inherent size. As an alternative, a small sized SEM, simply called a mini-SEM, is introduced even if the performance in terms of magnification and resolution is a little inferior to a classical thermal SEM. However, the size and fabrication cost is dramatically reduced, dedicating to opening a new market. The optical system in the mini-SEM is redesigned and specimen stage is quitely reduced and vertical axis is excluded. The design tools and calibration techniques to develope the mini-SEM are introduced and its performance is verified through numerical analysis experiments.