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http://dx.doi.org/10.4014/jmb.1003.03028

Establishment of an Axenic Culture of Microcystin-Producing Microcystis aeruginosa Isolated from a Korean Reservoir  

Han, Ah-Won (Department of Environmental Engineering, Chungbuk National University)
Oh, Kyoung-Hee (Department of Environmental Engineering, Chungbuk National University)
Jheong, Weon-Hwa (National Institute of Environmental Research)
Cho, Young-Cheol (Department of Environmental Engineering, Chungbuk National University)
Publication Information
Journal of Microbiology and Biotechnology / v.20, no.7, 2010 , pp. 1152-1155 More about this Journal
Abstract
In order to establish an axenic (bacteria-free) culture of Microcystis aeruginosa NIER 10039 isolated from a Korean reservoir, the culture was subjected to sequential treatment, including ultrasonication, washing, and addition of antibiotics. Three broad-spectrum antibiotics, namely, kanamycin, ampicillin, and imipenem, were applied separately in that order. Axenicity of the culture was confirmed by cultivation on bacterial media and observation under epifluorescence and scanning electron microscopes. We are the first to establish an axenic culture of a Microcystis strain isolated from Korean reservoirs and can be used in physiological and molecular studies to control toxic Microcystis blooms.
Keywords
Axenic culture; Microcystis; Korean reservoir; sequential treatment; antibiotics;
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Times Cited By Web Of Science : 2  (Related Records In Web of Science)
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