• Korean Journal of Plant Tissue Culture
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• v.22 no.6
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• pp.323-327
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• 1995

This experiment was carried out to observe the origin and developmental pattern of somatic embryos of Oenanthe javanica ($B^{L}.$) DC. The experiment included observation of embryogenic cells and their development stages by light microscope, transmission electron microscope and scanning electron microscope. The embryogenic cells, which were smaller than non-embryogenic cells in size with expanded nucleus and dense cytoplasm. When stained with hematoxylin, the embryogenic cells were readily distinguished from the non-embryogenic cells of which cell walls were stained with safranin. It was observed at somatic embryos developed from single cells on the epidermis of developing embryos or in the surface or inside of embryogenic clumps by segmentation pattern. Observation with a transmission electron microscope revealed that the embryogenic cells had dense cytoplasm expanded nucleus, small vacuoles, large amyloplasts containing starch grains, and abundant organelles including lipid bodies. Under a scanning electron microscope, embryogenic callus was shown to consist of very smaller cells than non-embryogenic cells in an orderly arrangement and covered with a net-like structure, while the non-embryogenic callus consisted of large cells, irregular in size and arrangement, and covered with a gelatin-like material.

• Proceedings of the Korean Society of Tribologists and Lubrication Engineers Conference
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• 2002.10b
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• pp.61-62
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• 2002

In order to clarify the contact mechanism between specimen surface and probe tip in the surface observation by the AFM (atomic force microscope) or the FFM (friction force microscope), several molecular dynamics simulations have been performed. In the simulation, a 3-dimensional simulation model is proposed where the specimen and the probe are assumed to consist of mono-crystal line copper and a carbon atom respectively and the effect of cantilever stiffness is also taken into considered. The surface observation process on a well-defined Cu{100} is simulated. The influences of cantilever stiffness on the reactive force images and the behavior of probe tip were evaluated. As a resuIt, several phenomena similar to those observed by the actual surface observation experiment, such as double-slip behavior and dispersion in the stick-slip wave period were observed.

• Journal of Information Processing Systems
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• v.15 no.1
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• pp.47-54
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• 2019

This study is concerned with the mechanism and structure of an optical microscope and an automatic multi-focus algorithm for automatically selecting sharp images from multiple foci of a cell. To obtain precise cell images quickly, a z-axis actuator with a resolution of $0.1{\mu}m$ was designed to control an optical microscope Moreover, a lighting control system was constructed to select the color and brightness of light that best suit the object being viewed. Cell images are captured by the instrument and the sharpness of each image is determined using Gaussian and Laplacian filters. Next, cubic spline interpolation and peak detection algorithms are applied to automatically find the most vivid points among multiple images of a single object. A cancer cell imaging experiment using propidium iodide staining confirmed that a sharp multipoint image can be obtained using this microscope. The proposed system is expected to save time and effort required to extract suitable cell images and increase the convenience of cell analysis.

• 한국전자현미경학회:학술대회논문집
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• 2005.11a
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• pp.150-152
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• 2005

• 한국전자현미경학회:학술대회논문집
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• 1998.05a
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• pp.68-68
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• 1998

• The Korean Journal of Mycology
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• v.17 no.1
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• pp.48-50
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• 1989

Observations of basidia in Korean Commercial Agaricus bisporus were made with Electron Scanning Microscope. One and two spored basidia were found, but this is not consistent with the other observation. Based on this observation, some sepeculation was made for breeding systems for Korean Commercial A. bisporus.

• Journal of Veterinary Clinics
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• v.7 no.2
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• pp.497-500
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• 1990

This study was carried out determine whether Unopette can be used for the observation of sperm morphology and sperm Concentration. Rabbit sperm and frozen-thawed bovine sperm were observed with phase contrast microscope after dilution with Unopette acooriding to duration of preservation at 3～5$^{\circ}C$. Sperm using Unopette showed high normal sperm(％) than sperm using hematoxylin-eosin until 48 hours. Sperm using Unopette revealed no difference in sperm concentration until 24 hours, as compared with control sperm. As a result, Unopette was assessed as appropriate solution for preservation in terms of morphological observation and sperm concentration.

• Proceedings of the Korean Society For Composite Materials Conference
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• 2003.04a
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• pp.211-214
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• 2003

This paper presents the microscopic observation results from the picture frame test using five-harness satin weave fabric composite. Aligned and misaligned specimens are observed to verify the exact tow deformation pattern such as tow interval and change in tow amplitude. To observe the micro-deformation of the fabric structure, appropriate specimens from picture frame test are sectioned and observed under the microscope. From the observation results, it is found that a picture frame test with a misaligned fibre orientation angle shows large differences in deformation between tensile and compressive tow directions.

• Journal of the Korean Society for Precision Engineering
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• v.21 no.2
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• pp.92-99
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• 2004

The errors can cause the serious loss of the performance of a precision machine system. In this paper, we proposed the method of allocating the alignment tolerances of the parts and applied this method to get the optimal tolerances of a Confocal Scanning Microscope. In general, tight tolerances are required to maintain the performance of a system, but a high cost of manufacturing and assembling is required to preserve the tight tolerances. The purpose of allocating the optimal tolerances is minimizing the cost while keeping the high performance of the system. In the optimal problem, we maximized the tolerances while maintaining the performance requirements. The Monte Carlo Method, a statistical simulation method, is used in tolerance analysis. Alignment tolerances of optical components of the confocal scanning microscope are optimized to minimize the cost and to maintain the observation performance of the microscope. We can also apply this method to the other precision machine system.

• Journal of Environmental Health Sciences
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• v.30 no.2
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• pp.108-114
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• 2004

Observation of microorganisms collected from contaminated soils has been mainly conducted by using microscopy. Microscopic measurement is occupied an important part of the microorganism experiment, and is used as an important tool to count microorganisms as well as to observe cellular form and mode of life in the field of soil microbe observation. In general, observation equipments for soil microbes consist of electron microscope, camera, frame grabber (image acquisition baud), and image analysis software. Because image analysis software should be linked with frame grabber most equipments have to be imported as the package form. Therefore, the observation system is very expensive and difficult to be operated. In this study, soil microbes＇ observation equipment with the vision system which is easy operated and cheaper than imported one was developed and tested. The efficiency of image capturing and data aquisition with developed frame grabber and software in this experiment was good enough to analyze the image of soil microorganism.