• Proceedings of the KSME Conference
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• 2001.06e
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• pp.100-106
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• 2001

The size distributions of electrospray droplets from the Taylor cone in cone-jet mode are directly measured by using a freezing method and a transmission electron microscope (TEM) image processing technique. These results are compared with the data obtained by an aerodynamic size spectrometer (TSI Aerosizer DSP). The use of glycerol seeded with NaI and a freezing method make it possible to sample droplets with their original sizes preserved. Since pictures of droplets are taken with TEM with very low vapor pressure of the solution, evaporation is suppressed by freezing. For liquid flow rates below 1 nl/sec, the measured droplet diameters by the TEM image processing technique and the aerosizer are in the range of 0.25 to $0.32{\mu}m$ and 0.30 to $0.40{\mu}m$, respectively. Comparing the TEM data with the aerosizer measurements, it has been revealed that the TEM image processing technique can afford more accurate values of droplet size distributions in the submicron range of 0.1 to $0.4{\mu}m$.

• Journal of the Korean Society for Nondestructive Testing
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• v.22 no.1
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• pp.14-21
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• 2002

The development of a new experimental method is required to easily observe the growth behavior of fatigue cracks. To satisfy the requirement, an image processing technique was introduced to fatigue testing. The length of surface fatigue crack could be successfully measured by the image processing system. At first, the image data of cracks were stored into the computer while the cyclic loading was interrupted. After testing, crack length was determined using an image processing software which was developed by authors. Various image processing techniques like a block matching method was applied tc the detection of surface fatigue cracks. By comparing the data measured by the image processing system with those by the manual measurement with a microscope, the effectiveness of the image processing system was established. If the proposed method is used to monitor and observe the crack growth behavior automatically, the time and efforts for fatigue test could be dramatically reduced.

• 제어로봇시스템학회:학술대회논문집
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• 2002.10a
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• pp.41.5-41
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• 2002

This paper presents a feature extraction algorithm for vision-based micromanipulation. In order to guarantee of the accurate micromanipulation, most of micromanipulation systems use vision sensor. Vision data from an optical microscope or high magnification lens have vast information, however, characteristics of micro image such as emphasized contour, texture, and noise are make it difficult to apply macro image processing algorithms to micro image. Grasping points extraction is very important task in micromanipulation because inaccurate grasping points can cause breakdown of micro gripper or miss of micro objects. To solve those problems and extract grasping points for micromanipulation...

• KIPS Transactions on Software and Data Engineering
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• v.5 no.6
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• pp.267-272
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• 2016

Microscope cell image is an important indicator for obtaining the biological information in a bio-informatics fields. Since human observers have been examining the cell image with microscope, a lot of time and high concentration are required to analyze cell images. Furthermore, It is difficult for the human eye to quantify objectively features in cell images. In this study, we developed HCS algorithm for automatic analysis of cell image using an OpenCV library. HCS algorithm contains the cell image preprocessing, cell counting, cell cycle and mitotic index analysis algorithm. We used human cancer cell (MKN-28) obtained by the confocal laser microscope for image analysis. We compare the value of cell counting to imageJ and to a professional observer to evaluate our algorithm performance. The experimental results showed that the average accuracy of our algorithm is 99.7%.

• Computers and Concrete
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• v.29 no.2
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• pp.107-115
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• 2022

With the improvements in computer technologies, utilization of image processing techniques has increased in many areas (such as medicine, defence industry, other industries etc.) Many different image processing techniques are used for surface analysis, detection of manufacturing defects, and determination of physical and mechanical characteristics of composite materials. In this study, cementitious composites were obtained by addition of Grounded Granulated Blast-Furnace Slag (GGBFS), Styrene Butadiene polymer (SBR), and Grounded Granulated Blast-Furnace Slag and Styrene Butadiene polymer together (GGBFS+SBR). Expanded Polystyrene (EPS) beads were added to these cementitious composites in different ratios (20%, 40% and 60%). The mechanical and physical characteristics of the composites were determined, and homogeneity indexes of the composites were determined by image processing techniques to determine EPS distribution forms in them. Physical and mechanical characteristics of the produced samples were obtained by applying consistency, density, water absorption, compressive strength (7 and 28 days), flexural strength (7 and 28 days) and tensile splitting strength (7 and 28 days) tests on them. Also, visual examination by using digital microscope, and image analysis by using image processing techniques with open source coded ImageJ program were performed. As a result of the study, it is determined that GGBFS and SBR addition strengthens the adhesion sites formed as it increases the adhesion power of the mixture and helps to get rid of the segregation problem caused by EPS. As a result of the image processing analysis it is demonstrated that GGBFS and SBR addition has positive contribution on homogeneity index.

• Journal of Biomedical Engineering Research
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• v.30 no.3
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• pp.191-198
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• 2009

Phlegm smear testing for acid-fast bacilli (AFB) requires careful examination of tubercle bacilli under a microscope to distinguish between positive and negative findings. The biggest weakness of this method is the visual limitations of the examiners. It is also time-consuming, and mistakes may easily occur. This paper proposes a method of identifying tubercle bacilli that uses a computer instead of a human. To address the challenges of AFB testing, this study designs and investigates image systems that can be used to identify tubercle bacilli. The proposed system uses an electronic microscope to capture digital images that are then processed through feature extraction, image segmentation, image recognition, and neural networks to analyze tubercle bacilli. The proposed system can detect the amount of tubercle bacilli and find their locations. This paper analyzes 184 tubercle bacilli images. Fifty images are used to train the artificial neural network, and the rest are used for testing. The proposed system has a 95.6% successful identification rate, and only takes 0.8 seconds to identify an image.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2009.06a
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• pp.333-334
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• 2009

• The Transactions of The Korean Institute of Electrical Engineers
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• v.57 no.10
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• pp.1876-1882
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• 2008

Nano manipulator is used to manufacture Carbon NanoTube(CNT) tips. Using nano manipulator, operator attaches a CNT at the apex of Atomic Force Microscope(AFM) tip, which requires a mastery of mechanics and long manufacture time. Nano manipulator is installed inside a Scanning Electron Microscope(SEM) chamber to observe the operation. This paper presents a control scheme for horizontal axes of nano manipulator via processing SEM image. Edges of AFM tip and CNT are first detected, and the position information so obtained is fed to control horizontal axes of nano manipulator. That is, a visual servoing loop is realized to control the axes more precisely in nano scale.

• Journal of the Korean Institute of Telematics and Electronics
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• v.27 no.12
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• pp.1889-1893
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• 1990

In this paper, ultrasonic microscope system has been constructed with the small aperturre acoustic lens and the angle controller, and the new type of B-scanultransonic imaging has been analyzed. The system with small aperture lens was used to detect flaw existing within the thick specimen and its resolution was in the range of one wavelength at interior plane of sample. The anle controller was used to excited surface acoustic wave or shear wave. In order to obtain B-scan image of the flaw existig at interior of solids, shear wave has been excited and backscattering signals from the flaw have been processed. In experimental results, B-scan ultransonic images have been obtain from the flaws of varable shape and measured flaw size from the images has been in good agreement with practical size in the range of 10% error.

• 제어로봇시스템학회:학술대회논문집
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• 2000.10a
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• pp.287-287
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• 2000

As a method to control the bacteria number in adequate level, a real time control system based on microscope image processing measurement for the bacteria is adopted. For the experiment, Ammonia-oxidizing bacteria such as Acinetobacter sp. are used. This paper proposed hybrid method combined watershed algorithm with adaptive automatic thresholding method to enhance segmentation efficiency of overlapped image. Experiments was done to show the effectiveness of the proposed method compared to traditional Otsu's method, Otsu's method with adaptive automatic thresholding method and human visual method.