• Title/Summary/Keyword: microscope image processing

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Precision measurement of a laser micro-processing surface using a hybrid type of AFM/SCM (하이브리드형 AFM/SCM을 이용한 레이저 미세 가공 표면 측정)

  • Kim, Jong-Bae;Kim, Kyeong-Ho;Bae, Han-Sung;Nam, Gi-Jung;Lee, Dae-Chul;Seo, Woon-Hak
    • Proceedings of the Korean Society of Laser Processing Conference
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    • 2006.11a
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    • pp.123-127
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    • 2006
  • Hybrid type microscope with a Scanning Confocal Microscope (SCM) and a shear-force Atomic Force Microscope (AFM) is suggested and preliminarily studied. A image of $120{\times}120{\mu}m^2$ is obtained within 1 second by SCM because scan speed of a X-axis and Y-axis are 1kHz and 1Hz, respectively. Shear-force AFM is able to correctly measure the hight and width of sample with a resolution 8nm. However, the scan speed is slow and it is difficult to distinguish a surface composed of different kinds of materials. We have carried out the measurement of total image of a sample by SCM and an exact analysis of each image by shear-force AFM.

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ELECTRO-MICROSCOPE BASED 3D PLANT CELL IMAGE PROCESSING METHOD

  • Lee, Choong-Ho;Umeda Mikio;Takesi Sugimoto
    • Proceedings of the Korean Society for Agricultural Machinery Conference
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    • 2000.11b
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    • pp.227-235
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    • 2000
  • Agricultural products are easily deformable its shape because of some external forces. However, these force behavior is difficult to measure quantitatively. Until now, many researches on the mechanical property was performed with various methods such as material testing, chemical analysis and non-destructive methods. In order to investigate force behavior on the cellular unit of agricultural products, electro-microscope based 3D image processing method will contribute to analysis of plant cells behavior. Before image measurement of plant cells, plant sample was cut off cross-sectioned area in a size of almost 300-400 ${\mu}$ m units using the micron thickness device, and some of preprocessing procedure was performed with fixing and dyeing. However, the wall structure of plant cell is closely neighbor each other, it is necessary to separate its boundary pixel. Therefore, image merging and shrinking algorithm was adopted to avoid disconnection. After then, boundary pixel was traced through thinning algorithm. Each image from the electro-microscope has a information of x,y position and its height along the z axis cross sectioned image plane. 3D image was constructed using the continuous image combination. Major feature was acquired from a fault image and measured area, thickness of cell wall, shape and unit cell volume. The shape of plant cell was consist of multiple facet shape. Through this measured information, it is possible to construct for structure shape of unit plant cell. This micro unit image processing techniques will contribute to the filed of agricultural mechanical property and will use to construct unit cell model of each agricultural products and information of boundary will use for finite element analysis on unit cell image.

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Application of Image Processing to Determine Size Distribution of Magnetic Nanoparticles

  • Phromsuwan, U.;Sirisathitkul, C.;Sirisathitkul, Y.;Uyyanonvara, B.;Muneesawang, P.
    • Journal of Magnetics
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    • v.18 no.3
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    • pp.311-316
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    • 2013
  • Digital image processing has increasingly been implemented in nanostructural analysis and would be an ideal tool to characterize the morphology and position of self-assembled magnetic nanoparticles for high density recording. In this work, magnetic nanoparticles were synthesized by the modified polyol process using $Fe(acac)_3$ and $Pt(acac)_2$ as starting materials. Transmission electron microscope (TEM) images of as-synthesized products were inspected using an image processing procedure. Grayscale images ($800{\times}800$ pixels, 72 dot per inch) were converted to binary images by using Otsu's thresholding. Each particle was then detected by using the closing algorithm with disk structuring elements of 2 pixels, the Canny edge detection, and edge linking algorithm. Their centroid, diameter and area were subsequently evaluated. The degree of polydispersity of magnetic nanoparticles can then be compared using the size distribution from this image processing procedure.

Measurement of Surface Crack Length Using Image Processing Technology (영상처리기법을 이용한 표면균열길이 측정)

  • Nahm, Seung-Hoon;Kim, Yong-Il;Kim, Si-Cheon;Ryu, Dae-Hyun
    • Proceedings of the KSME Conference
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    • 2001.06a
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    • pp.96-101
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    • 2001
  • The development of a new experimental method is required to easily observe the growth behavior of fatigue cracks. To satisfy the requirement, an image processing technique was introduced to fatigue testing. The length of surface fatigue crack could be successfully measured by the image processing system. At first, the image data of cracks were stored into the computer while the cyclic loading was interrupted. After testing, crack length was determined using image processing software which was developed by ourselves. Block matching method was applied to the detection of surface fatigue cracks. By comparing the data measured by image processing system with the data measured by manual measurement with a microscope, the effectiveness of the image processing system was established. If the proposed method is used to monitor and observe the crack growth behavior automatically, the time and efforts for fatigue test could be dramatically reduced.

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Tree aging observation of XLPE by image processing (화상처리에 의한 XLPE의 트리열화관측)

  • 임장섭;김태성;길촌승
    • Electrical & Electronic Materials
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    • v.8 no.5
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    • pp.551-557
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    • 1995
  • For the observation of treeing, a visual measurement with an optical microscope has been used to explain breakdown mechanism in high-voltage systems. The conventional directed visual method of tree aging observation is difficult to measure in short time processing, and it is impossible to analyze on tree degradation area, progressed direction, tree pattern, etc. By using an image processing technique, the tree features which appear immediately after the tree initiation as well as changes in the configuration of the tree can be easily measured and observed than using the conventional visual methods. In this paper, we have developed a tree observating system by using image processing for tree growth, degradation area and other treeing progress. As an experimental result, it can be concluded that the image processing method is a more effective alternative than directed visual observation method. As a matter of fact, it is possible to record the image of tree propagation immediately after its first appearance and explain the characteristics of tree growth froth the computer processing image.

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A Study on an Automatic Multi-Focus System for Cell Observation

  • Park, Jaeyoung;Lee, Sangjoon
    • Journal of Information Processing Systems
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    • v.15 no.1
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    • pp.47-54
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    • 2019
  • This study is concerned with the mechanism and structure of an optical microscope and an automatic multi-focus algorithm for automatically selecting sharp images from multiple foci of a cell. To obtain precise cell images quickly, a z-axis actuator with a resolution of $0.1{\mu}m$ was designed to control an optical microscope Moreover, a lighting control system was constructed to select the color and brightness of light that best suit the object being viewed. Cell images are captured by the instrument and the sharpness of each image is determined using Gaussian and Laplacian filters. Next, cubic spline interpolation and peak detection algorithms are applied to automatically find the most vivid points among multiple images of a single object. A cancer cell imaging experiment using propidium iodide staining confirmed that a sharp multipoint image can be obtained using this microscope. The proposed system is expected to save time and effort required to extract suitable cell images and increase the convenience of cell analysis.

Measurement of Porosity by EPMA-EDS Image Processing

  • Hung, Minhui;Li, Xiangting;Xia, Jiyu;Ding, Chuanxian
    • Journal of the Korean Vacuum Society
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    • v.6 no.S1
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    • pp.66-69
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    • 1997
  • Porosity is one important characteristic feature and structural index of sprayed coatings. A method of measurement of porosity, EPMA-EDS image processing is developed in the paper. The characteristics of pores can be determined by processing of the image obtained from an electron microscope via VISTA, Not only the porosity can be presented but also the statistical result of pore size distribution. Finally it can be drawn from this paper that EPMA-EDS is a quite effective method to completely characterize the pores in plasma sprayed coatings.

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