• Title/Summary/Keyword: microorganism growth

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Analysis of the Survey on the Consumer's Knowledge and Laundry Habits to Microorganisms Living in Clothing (의류 중의 미생물에 대한 소비자의 지식과 세탁습관 실태조사 분석)

  • 최해운;정찬진;박명자
    • The Research Journal of the Costume Culture
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    • v.10 no.6
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    • pp.781-792
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    • 2002
  • Microorganisms living in clothing cause damage to fabric as well as unhygienic conditions with unpleasant odor fur wearers. Removal or growth of microorganisms are affected by the conditions during washing and storage. The purpose of this research was to study the consumer's knowledge and habits in laundering with respect to microorganisms in clothing. For survey method, questionnaires were administered to 580 housewives, age of 20∼60s living in Seoul. Employing 479 respondents, the data were analyzed by using descriptive statistics. The results are as follows: The level of knowledge about microorganisms of clothing was high in general, but wasn't expert level. Many people had experienced damages of textiles, clothing and unpleasant odor due to microorganisms. Fabric softeners and bleaches were rarely used for disinfection but usually used for antistatic, whitening or removal of stains. There was no relationship between laundering habits, the knowledge of microorganism, and experience of clothing damage by microorganism.

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Identification and Physiological Characteristics of Microorganism Isolated from Spoiled Sweetened Adzuki Ann (부패된 팥앙금으로부터 분리된 미생물의 동정과 그 균의 생리적 특성)

  • Lee, Tae-Kyoo;Roh, Min-Hwan
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.35 no.10
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    • pp.1456-1460
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    • 2006
  • In order to determine the causes of sweetened adzuki ann spoilage, the characteristics of microorganism isolated from spoiled adzuki ann were investigated. The isolated microorganism was gram-positive, roil-shaped and shore-forming bacteria; its surface was smooth and glazed. From the results of the assimilation test of 46 different biochemicals by the Vitec 2 Compact test and comparison of the cellular wall composition of fatty acid by the data bank of Midi sherlock system, the microorganism was identified as Bacillus subtilis, D-value of the B. subtilis spore was 4.85 min at $115^{\circ}C$, 0.69 min at $121^{\circ}C$ and 0.48 min at $125^{\circ}C$; Z-value was 9.71. The Bacillus subtilis growth was not observed below water activity of 0.92 at $45^{\circ}C$. However, bacteria growth increased gradually as water activity increased above 0.93.

Effect of Microorganism, Vitabio on Growth and Quality of Leaf Lettuce (Vitabio 土壤微生物劑 處理가 葉상치 收量 및 品贊에 미치는 영향)

  • Kim, Kyung-Je;Lee, Byung-Moo
    • Korean Journal of Organic Agriculture
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    • v.12 no.3
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    • pp.345-352
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    • 2004
  • This study was carried out to investigate the effect of soil-born microorganism, vitabio on growth of leaf lettuce in the vinyl house. Total weights of leaf lettuce treated with vitabio showed difference compared with untreated leaf lettuce. Sugar content was also increased. Mineral contents of leaf lettuce showed no difference between treated with vitabio and untreated with vitabio. Exchange Capacity (EC) and Organic Matter (OM) in chemical properties of soil treated with vitabio showed higher than soil in untreated vitabio. Vitabio treated soil contained much more microorganisms such as Bacteria, Actinomycetes, Hyphomycetes, Bacillus sp. Pseudomonas sp. after harvest.

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Zygotorulaspora cornina sp. nov. and Zygotorulaspora smilacis sp. nov., Two Novel Ascomycetous Yeast Species Isolated from Plant Flowers and Fruits

  • Ahn, Chorong;Kim, Minkyeong;Kim, Changmu
    • Mycobiology
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    • v.49 no.5
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    • pp.521-526
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    • 2021
  • Three isolates belonging to the ascomycetous genus Zygotorulaspora were obtained from the fruits of Cornus officinalis and Smilax china, and flowers of Dendranthema zawadskii var. latilobum in Gongju-si, Korea. Phylogenetic Analyses of the LSU D1/D2 domain and ITS region sequences supported the recognition of two new species: Zygotorulaspora cornina sp. nov. (type strain NIBRFGC000500475 = KACC93346PPP) and Zygotorulaspora smilacis sp. nov. (type strain NIBRFGC000500476 = KACC93347PPP). The two novel species revealed no growth on D-Galactose, unlike the other six species in the genus Zygotorulaspora. They are distinguished from each other by their phylogenetic differences and phenotypic characteristics such as assimilation of xylitol, 5-keto-D-gluconate, and ethanol. All species in the genus Zygotorulaspora including the two novel species have phenotypic traits of genus Zygotorulaspora: asci are persistent, sucrose and raffinose are assimilated, and m-inositol is not required for growth, and they are mainly associated with plants.

Isolation and Characterization of Burkholderia cepacia strain YJK2, Antagonistic Microorganism of Paprika Pathogens (파프리카 병원균들에 대한 길항미생물, Burkholderia cepacia strain YJK2의 분리 및 특성)

  • Yang, Soo-Jeong;Kim, Hyung-Moo;Ju, Ho-Jong
    • Korean Journal of Organic Agriculture
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    • v.23 no.1
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    • pp.133-148
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    • 2015
  • Although several adverse effects have been increased in recent years, synthetic agro-chemicals have been widely used to control diseases on paprika. This research was conducted to isolate and to characterize the antagonistic microorganism to control major paprika diseases, gray mold rot, fruit and stem rot, phytophthora blight, sclerotium rot, and wilt disease. Analysis of the fatty acid and analysis of the 16S rDNA gene sequence revealed that YKJ2 isolated in this research belongs to a group of Burkholderia cepacia. Specially, 16S rDNA gene sequence of YKJ2 showed 99% of sequence similarity with B. cepacia. Observation through the optical microscope revealed that YKJ2 was effective on suppression of the spore germination and the hyphal growth of pathogens. YKJ2 treatment on pathogens induced marked morphological changes like hyphal swelling and degradation of cell wall. In the case of phytophthora blight, the zoosporangium formation was restrained. On the basis of the results of this study, we propose that an antagonistic microorganism, B. cepacia, found in this study naming as "B. cepacia strain YKJ2" and has great potential as one of biological control agents against major diseases of paprika.

Investigated of Mathematical Model for the Specific Growth Rate of Ethanol Producing Microorganism, Saccharomyces cerevisiae ATCC 24858 (에탄올 생산 균주 Saccharomyces cerevisiae ATCC 248858의 비성장속도에 관한 수학적 모형연구)

  • 김휘동;허병기
    • KSBB Journal
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    • v.13 no.6
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    • pp.730-734
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    • 1998
  • The mathematical model of specific growth rate of Saccharomyces cerevisiae ATCC 24858 is proposed as a function of sugar and ethanol concentrations by the combination of Andrew's equation and Aiba's equation. The maximum concentration of sugar Sm, which was the highest concentration of sugar not having any effect on the growth inhibition, was 150 g/L and the substrate inhibition was expressed as a function of (S-Sm). The maximum specific growth inhibition, was 150 g/L and the substrate inhibition was expressed as a function of (S-Sm). The maximum specific growth rate ${\mu}m$, Monod's constant Ks, and Andrew's inhibition constant KI were 0.49 hr-1, 19 g/L, and 139 g/L, respectively. The maximum ethanol concentration, Pm, which did not show any inhibition effect on the specific growth rate was found to be 2 g/L. Therefore, the ethanol inhibition was represented as a function of (P-Pm). The final mathematical model for the specific growth rate of the microorganism in this work is proposed as the following. And the average percent of errors between the calculated specific growth rate and the experimental values was 5.96%.

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Antimicrobial Effect of Ampelopsis brevipedunculata Extracts On Food Spoilage or Foodborne Disease Microorganism (개머루덩굴 추출물의 식품부패 및 병원성 미생물에 대한 항균효과)

  • Choi, Moo-Young;Rhim, Tae-Jin
    • Korean Journal of Plant Resources
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    • v.23 no.5
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    • pp.430-435
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    • 2010
  • This study was performed to investigate the antimicrobial effects of Ampelopsis brevipedunculata ethanol-extract on food spoilage or foodborne disease microorganism. The antimicrobial activity of Ampelopsis brevipedunculata extract was determined by a paper disc method. The extract exhibited growth inhibiting activities in a concentration dependent manner on 7 species of microorganisms. The extract of Ampelopsis brevipedunculata showed the highest antimicrobial activity against Escherichia coli O157:H7. The growth inhibitory effects of the extract at the concentration of 250, 500, 1,000, 2,000 mg/L on food poisoning microorganism were determined against Salmonella typhimurium, Yersinia enterocolitica, Escherichia coli O157:H7 and Staphylococcus aureus. The growth of microorganisms was not affected by the extract at the concentration up to 250 mg/L, but was significantly (p<0.05) inhibited by the extract at the concentration higher than 500 mg/L. The results in the present study demonstrate antimicrobial effects of Ampelopsis brevipedunculata ethanol-extract against foodborne pathogens, suggesting that Ampelopsis brevipedunculata could be an effective natural antibacterial agent in food.

Microbial Contamination Levels of Red Pepper Powder Purchased in Gyeonggi Province and Changes in Characteristics According to the Storage Method

  • Choi, Yu-Mi;Hwang, Sun-Il;Park, Geon-Yeong;Kim, Sang-Tae;Lee, Hyo-Kyung;Lim, Hye-Won;Kim, Hye-Young;Ham, Hyun-Kyung;Park, Yong-Bae
    • Journal of Food Hygiene and Safety
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    • v.36 no.5
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    • pp.382-391
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    • 2021
  • The purpose of this study was to investigate the microbial contamination of red pepper powder distributed within Gyeonggi province in 2020 according to the place of purchase, the country of origin, and whether the HACCP certification and sterilization were conducted, and to evaluate the change of quality according to the storage method. Upon collecting and analyzing 100 samples, Bacillus cereus was detected in 3 cases (2 cases in large supermarkets and 1 case in traditional markets) and Clostridium perfringens in 27 cases (9 cases in large supermarkets and 18 cases in traditional markets). The levels of the total aerobic bacteria were not significantly different between the red pepper powder purchased from large supermarkets and traditional markets. However, the frequency of red pepper powder exceeding 7 log CFU/g of total aerobic bacteria was higher in traditional markets than in large supermarkets. Microbial quality was not significantly different regardless of the storage temperature (30℃, 4℃, -20℃) and the packaging method (zipper bag and clean bag) after 7 months of purchase. However, the moisture contents and ASTA color value of red pepper powder stored at 30℃ decreased remarkably after 3 months of storage. It is desirable to store red pepper powder in a refrigerator or freezer in order to maintain its quality during long-term storage.

Characteristics and Model for Growth of Rhizopus oryzae on the Simulated Gas-solid Interface

  • Jia, Shiru;Kong, Rixiang;Dong, Huijun;Kwun, Kyu-Hyuk;Kim, Sun-Il;Cho, Ki-An;Choi, Du Bok
    • Korean Journal of Environmental Biology
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    • v.22 no.4
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    • pp.494-500
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    • 2004
  • In order to investigate the effect on morphology of Rhizopus oryzae and production of lactic acid, various interface materials were used. Morphology of fungal showed sheet and flock when resin was added. The production of lactic acid was increased dramatically when interface materials were added. Furthermore, the effect of resin was more significant than that of others. It was assumed that interface materials could absorb substrate and microorganism together, so microorganism was not inhibited by substrate. The effect of static electric field on the interface culture was studied. When the exerting potential was 6.78 voltage, the biomass y was obviously higher than that of zero voltage. A simulated gas-solid interface system was developed to study the growth and two phases model for the growth of Rhizopus oryzae was build up that depended on the symmetric branching theory. An important parameter F was researched. The results indicated that the value of F had obvious difference at exponential and deceleration period, respectively.

Characteristic of Alcohol Fermentation by the Culture of High Cell Density (Functional Relationship among Specific Growth Rate, Sugar Concentration, Cell Concentration and Alcohol Concentration) (고농도 균체배양에 의한 알콜발효특성 규명 (비성장속도와 당농도, 균체농도, 알콜농도의 함수관계))

  • 허병기;김형철양지원목영일
    • KSBB Journal
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    • v.6 no.1
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    • pp.85-90
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    • 1991
  • Experiments of alcohol fermentation of the yeast,K. fragi1is CBS 1555 were performed to obtain the following results. In these experiments, the initial concentrations of sugar which was composed of inulin and fructose as weight ratio of one to one were 30, 50, 75, 100 and 150g/l and the initial densities of the microorganism were less than 0.5g/l, 10g/1 to 15g/1, and 50g/l. The functional relationship among specific growthrates, sugar concentrations, and alcohol concentrations could be expressed by Aiba-shoda equation and the specific growth rate represented the trend that decreased with increase in the initial concentration of the microorganism. Also, $\mu$max and Ks of Monod's equation could be expressed as the function of initial cell concentration like the following equations. $\mu$max=0.8-0.008X Ks=0.54X+8 In the region that sugar, alcohol and cell concentrations were 10g/1 to 120g/l, 0g/l to 60g/l and 0.5g/l to 50g/l respectively, the differences between the experimental values and the calculated ones for specific growth rate approached to 40% with respect to experimental values at the worst cases, but in most cases, those were distributed in the range of less than 20%.

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