Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
권호 표지

Antimicrobial Effect of Ampelopsis brevipedunculata Extracts On Food Spoilage or Foodborne Disease Microorganism

개머루덩굴 추출물의 식품부패 및 병원성 미생물에 대한 항균효과

  • Choi, Moo-Young (Dept. of Food and nutrition, Sangji University) ;
  • Rhim, Tae-Jin (Dept. of Biotechnology in Division of Animal and Life Resources, Sangji University)
  • 최무영 (상지대학교 식품영양학과) ;
  • 임태진 (상지대학교 동물생명자원과학부)
  • Received : 2010.06.28
  • Accepted : 2010.10.02
  • Published : 2010.10.30
Download PDF
⟨ Previous Next ⟩

Abstract

This study was performed to investigate the antimicrobial effects of Ampelopsis brevipedunculata ethanol-extract on food spoilage or foodborne disease microorganism. The antimicrobial activity of Ampelopsis brevipedunculata extract was determined by a paper disc method. The extract exhibited growth inhibiting activities in a concentration dependent manner on 7 species of microorganisms. The extract of Ampelopsis brevipedunculata showed the highest antimicrobial activity against Escherichia coli O157:H7. The growth inhibitory effects of the extract at the concentration of 250, 500, 1,000, 2,000 mg/L on food poisoning microorganism were determined against Salmonella typhimurium, Yersinia enterocolitica, Escherichia coli O157:H7 and Staphylococcus aureus. The growth of microorganisms was not affected by the extract at the concentration up to 250 mg/L, but was significantly (p<0.05) inhibited by the extract at the concentration higher than 500 mg/L. The results in the present study demonstrate antimicrobial effects of Ampelopsis brevipedunculata ethanol-extract against foodborne pathogens, suggesting that Ampelopsis brevipedunculata could be an effective natural antibacterial agent in food.

본 연구는 천연 식품보존료 개발의 일환으로 한약 재료로 이용되고 있는 개머루덩굴을 에탄올로 추출하여 식품부패 및 병원성 미생물에 대한 항균활성을 조사하였다. 개머루덩굴 에탄올 추출물은 식품부패 및 병원성 미생물에 대해 농도 의존적으로 항균효과를 보였으며, 그 중에서 Escherichia coli O157:H7 균에 대해 가장 높은 항균효과를 보였다. 또 개머루덩굴 에탄올 추출물이 식중독 유발세균의 성장에 미치는 효과를 검정하기 위해 Salmonella typhimurium, Yersinia enterocolitica, Staphylococcus aureus, Escherichia coli O157:H7 균들의 배양액에 개머루덩굴 에탄올 추출물의 농도를 250, 500, 1,000, 2,000 mg/L 각 농도별로 첨가하여 생육을 조사한 결과 250 mg/L까지는 대조군과 별 차이를 보이지 않았으나 500 mg/L 이상에서는 유의적인(p<0.05) 감소를 관찰할 수 있었다. 본 연구결과는 개머루덩굴 에탄올 추출물이 식중독을 유발시키는 세균에 대하여 우수한 항균작용을 나타내고 있으며, 따라서 개머루덩굴이 효과적인 천연보존료로서 이용될 수 있음을 시사하고 있다.

Keywords

References

  1. Bae, J. H. 2005. Antimicrobial Effect of Hedyotis diggusa Extracts on Food-Borne Pathogens. J. Korean Soc Food Sci Nutr. 34(1): 107-112(in Korean). https://doi.org/10.3746/jkfn.2005.34.1.107
  2. Bae, J. H., M.S. Kim and E. H. kang. 2005. Antimicrobial Effect of Lonicerae Flos Extracts on Food-borne Pathogens. Korean J. Food Sci. Technol. 37(4): 642-647(in Korean).
  3. Bae, J. H., H. J. Jang and J. I. Jung. 2005. Antimicrobial Effect of Rubia akane Nakai Extract on Food-Borne Pathogens. J. Korean Soc Food Sci. Nutr. 34(3): 389-394(in korean). https://doi.org/10.3746/jkfn.2005.34.3.389
  4. Choi, M. Y. 2010. Antibacterial Acivity of Euphorbia humifusa Extracts on Food-Borne Pathogenic Bacteria. Korean J. Community Living Science. 21(1): 13-18(in Korean).
  5. Choi, M. Y. and T. J. Rhim. 2008. Antimicrobial Effect of Oregano(Origanium majorana L.)Extract on Food-borne Pathogens. Korean J. Plant Res. 21(5): 352-356(in Korean).
  6. Choi, O.B., G.S. Yoo and K. H. Park. 1999. Antioxidative and antimicrobial effects of water extracts with Castanea crenata leaf tea. korean J. Food Sci. technol. 31(4): 1128-1131(in Korean).
  7. Chung, K. S., S. Y. Kang and J. Y. Kim. 2003. The Antibacterial Activity of Garlic Juice Against Pathogenic Bacterial and Lactic Acid Bacteria. Kor. J. Microbial. Biotechnol. 31(1). 32-35(in Korean).
  8. Chung, H. J. 2000. Antioxidative and antimicrobial activities of Opuntia ficus indica var. saboten. Korean J. Soc. Food Sci. 16(2):160-166(in Korean).
  9. Chung, H. J. and K. L. Noh. 2000. Screening of electron donating ability, antibacterial activity and nitrite scavenging effect of some herbal extracts. Korean J. Soc. Food Sci. 16(4), 372-377(in Korean).
  10. Jeong, M. R., J. D. Cha and Y. E. Lee. 2005. Antibacterial Activity of Korean Fig(Ficus carica L.)against Food Poisoning Bacteria. Korean J Food Cookery Sci. 21(1): 84-93(in Korean).
  11. Kim, M. H., S. Y. Kim, W. S. Shin and J. S. Lee. 2003. Antimicrobial Activity of Garlic Juice against Escherichia coli O157:H7. Korean J. Food Sci. Technol. 35(4): 752-755 (in Korean).
  12. Kim, Y. D., S. K. Kang, O. J. Choi, H. C. Lee, M. J. Jang and S. C. Shin. 2000. Screening of Antimicrobial Activity of Chopi (Zanthoxylum piperitum A.P.DC.) Extract. J. Korean Soc. Food Sci. Nutr. 29(6): 1116-1122(in Korean).
  13. Koh, M. S. 2004. Antimicrobial Activity of Saururus chinensis Baill Extract. J Korean Soc Food Sci Nutr. 33(7): 1098-1105 (in korean). https://doi.org/10.3746/jkfn.2004.33.7.1098
  14. Lee H. and J. Y. Lin 1988. Antimutagenic activity of extracts from anticancer drugs in Chinese medicine. Mutat. Res. 204:229-234. https://doi.org/10.1016/0165-1218(88)90093-6
  15. Oh, D. H., M. K. Lee and B. K. Park. 1999. Antimicrobial Activities of Commercially Available Tea on the Harmful Foodborne Organisms. J. Korean Soc. Food Sci. Nutr. 28(1): 100-106(in Korean).
  16. Park, C. S. 1997. Effect of Spices on the Growth of pathogenic Bacteria..Korean J. Soc. Food Sci. 13(3): 330-337(in Korean).
  17. Wu M. J., J. H. Yen, L. Wang and C. Y. Weng. 2004. Antioxidant activity of Porcelainberry (Ampelopsis brevipedunculata (Maxim.) Trautv.. Am. J. Chin. Med. 32:681-693. https://doi.org/10.1142/S0192415X04002387
  18. Yabe N. and H. Matsui. 2000. Ampelopsis brevipedunculata (Vitaceae) extract inhibits a progression of carbon tetrachlorideinduced hepatic injury in the mice. Phytomedicine 7:493-498. https://doi.org/10.1016/S0944-7113(00)80035-5
  19. Yabe N., K. Tanaka and H. Matsui. 1988. An ethanol-extract of Ampelopsis brevipedunculata (Vitaceae) berries decreases ferrous iron-stimulated hepatocyte injury in culture. J. Ethnopharmacol. 59:147-159.
  20. Yang, E. J. J. Han and I. S. Lee. 1999. Antimicrobial activity of medicinal herbs extracts on cooling preservation foods. Korean J. Postharvest Sci. Technol. 6(1):110-114(in Korean).