• 복식문화연구
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• 제10권6호
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• pp.781-792
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• 2002

Microorganisms living in clothing cause damage to fabric as well as unhygienic conditions with unpleasant odor fur wearers. Removal or growth of microorganisms are affected by the conditions during washing and storage. The purpose of this research was to study the consumer's knowledge and habits in laundering with respect to microorganisms in clothing. For survey method, questionnaires were administered to 580 housewives, age of 20∼60s living in Seoul. Employing 479 respondents, the data were analyzed by using descriptive statistics. The results are as follows: The level of knowledge about microorganisms of clothing was high in general, but wasn't expert level. Many people had experienced damages of textiles, clothing and unpleasant odor due to microorganisms. Fabric softeners and bleaches were rarely used for disinfection but usually used for antistatic, whitening or removal of stains. There was no relationship between laundering habits, the knowledge of microorganism, and experience of clothing damage by microorganism.

• 한국식품영양과학회지
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• 제35권10호
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• pp.1456-1460
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• 2006

변질된 팥앙금에서 분리한 균주는 광학 및 전자현미경을 통하여 관찰한 결과 그람양성 간균으로 포자를 형성하고 형성된 집락은 원형으로 점질성의 물질을 생산하는 mucoid type이었으며, 표면은 smooth 하였고 광택을 나타내었다. 미생물 동정 시스템($MicroLog^{TM}$ System, Release 4)인 VITEK 2 COMPACT을 이용하여 분리된 균주의 46개의 생화학적 분석을 비교한 결과 팥앙금의 부패 원인균은 Bacillus subtilus로 동정 (97.0%)되었으며, 보다 더 정확한 동정을 위하여 부패균의 세포벽 지방산 조성을 분석하여 Midi sherlock시스템의 databank와 비교 분석시 그 유사성이 0.724로 분석되었다. Bacillus subtilus의 포자의 D-value는 $115^{\circ}C$에서의 4.85분이 었고, $121^{\circ}C$에서는 0.69분, $125^{\circ}C$에서는 0.48분이었으며, Z-value는 $9.71^{\circ}C$이었다. $45^{\circ}C$에서 수분활성도에 따른 분리균의 생육 상태를 관찰한 결과 수분활성도 0.92이하에서는 균이 증식되지 않았고, 0.94에서는 증식되는 것으로 관찰되었다.

• 한국유기농업학회지
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• 제12권3호
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• pp.345-352
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• 2004

This study was carried out to investigate the effect of soil-born microorganism, vitabio on growth of leaf lettuce in the vinyl house. Total weights of leaf lettuce treated with vitabio showed difference compared with untreated leaf lettuce. Sugar content was also increased. Mineral contents of leaf lettuce showed no difference between treated with vitabio and untreated with vitabio. Exchange Capacity (EC) and Organic Matter (OM) in chemical properties of soil treated with vitabio showed higher than soil in untreated vitabio. Vitabio treated soil contained much more microorganisms such as Bacteria, Actinomycetes, Hyphomycetes, Bacillus sp. Pseudomonas sp. after harvest.

• Mycobiology
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• 제49권5호
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• pp.521-526
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• 2021

Three isolates belonging to the ascomycetous genus Zygotorulaspora were obtained from the fruits of Cornus officinalis and Smilax china, and flowers of Dendranthema zawadskii var. latilobum in Gongju-si, Korea. Phylogenetic Analyses of the LSU D1/D2 domain and ITS region sequences supported the recognition of two new species: Zygotorulaspora cornina sp. nov. (type strain NIBRFGC000500475 = KACC93346P^PP) and Zygotorulaspora smilacis sp. nov. (type strain NIBRFGC000500476 = KACC93347P^PP). The two novel species revealed no growth on D-Galactose, unlike the other six species in the genus Zygotorulaspora. They are distinguished from each other by their phylogenetic differences and phenotypic characteristics such as assimilation of xylitol, 5-keto-D-gluconate, and ethanol. All species in the genus Zygotorulaspora including the two novel species have phenotypic traits of genus Zygotorulaspora: asci are persistent, sucrose and raffinose are assimilated, and m-inositol is not required for growth, and they are mainly associated with plants.

• 한국유기농업학회지
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• 제23권1호
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• pp.133-148
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• 2015

Although several adverse effects have been increased in recent years, synthetic agro-chemicals have been widely used to control diseases on paprika. This research was conducted to isolate and to characterize the antagonistic microorganism to control major paprika diseases, gray mold rot, fruit and stem rot, phytophthora blight, sclerotium rot, and wilt disease. Analysis of the fatty acid and analysis of the 16S rDNA gene sequence revealed that YKJ2 isolated in this research belongs to a group of Burkholderia cepacia. Specially, 16S rDNA gene sequence of YKJ2 showed 99% of sequence similarity with B. cepacia. Observation through the optical microscope revealed that YKJ2 was effective on suppression of the spore germination and the hyphal growth of pathogens. YKJ2 treatment on pathogens induced marked morphological changes like hyphal swelling and degradation of cell wall. In the case of phytophthora blight, the zoosporangium formation was restrained. On the basis of the results of this study, we propose that an antagonistic microorganism, B. cepacia, found in this study naming as "B. cepacia strain YKJ2" and has great potential as one of biological control agents against major diseases of paprika.

• KSBB Journal
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• 제13권6호
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• pp.730-734
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• 1998

An이ew식과 Aiba삭을 조합하여 에탄올 생산단주인 Saccharomyces cerevisiae ATCC 24858의 비성장속도를 당농도와 에탄올 농도의 함수로 표현하였다. 기침의 저해영향을 받지 않는 최대 당농도 $S_m$은 150 g/L이며 기질의 저해영향은 기질농도 S와 $S-S_{max}$항의 함수로 표현되었다. 최대 비성장 속도 ${\mu}max 는 0.49 hr^{-1}, Monod상수 K_s$는 19 g/L, Andrew식의 기질저해상수 $K_1$는 139 g/L이였다. 또한 비성장속도에 영향을 마치지 앓는 최대알콜농도 Pm이 존재하였으며 그 값은 2 g/L 이였다. 따라서 Aiba식에서 비성장속도에 영향을 미치는 에탄올 농도항은 P-Pm으보 표현되었다. 본 연구의 알코올생산균주에 대한 비성장속도의 완성된 수식은 디음과 같으며 이 수식에 위한 계산값은 평균오차 6% 내외의 범위에서 실험값과 일치하였다.

• 한국자원식물학회지
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• 제23권5호
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• pp.430-435
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• 2010

본 연구는 천연 식품보존료 개발의 일환으로 한약 재료로 이용되고 있는 개머루덩굴을 에탄올로 추출하여 식품부패 및 병원성 미생물에 대한 항균활성을 조사하였다. 개머루덩굴 에탄올 추출물은 식품부패 및 병원성 미생물에 대해 농도 의존적으로 항균효과를 보였으며, 그 중에서 Escherichia coli O157:H7 균에 대해 가장 높은 항균효과를 보였다. 또 개머루덩굴 에탄올 추출물이 식중독 유발세균의 성장에 미치는 효과를 검정하기 위해 Salmonella typhimurium, Yersinia enterocolitica, Staphylococcus aureus, Escherichia coli O157:H7 균들의 배양액에 개머루덩굴 에탄올 추출물의 농도를 250, 500, 1,000, 2,000 mg/L 각 농도별로 첨가하여 생육을 조사한 결과 250 mg/L까지는 대조군과 별 차이를 보이지 않았으나 500 mg/L 이상에서는 유의적인(p<0.05) 감소를 관찰할 수 있었다. 본 연구결과는 개머루덩굴 에탄올 추출물이 식중독을 유발시키는 세균에 대하여 우수한 항균작용을 나타내고 있으며, 따라서 개머루덩굴이 효과적인 천연보존료로서 이용될 수 있음을 시사하고 있다.

• 한국식품위생안전성학회지
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• 제36권5호
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• pp.382-391
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• 2021

2020년 경기도내 유통 중인 고춧가루의 미생물 오염도를 구매 장소, 원산지, HACCP 및 살균 여부에 따라 조사하고, 보관방법에 따른 품질 변화를 연구하였다. 유통 중인 고춧가루 100건을 수거하여 검사한 결과 3건(대형마트 2건, 재래시장 1건)에서 Bacillus cereus가 검출되었으며, 27건(대형마트 9건, 재래시장 18건)에서 Clostridium perfringens가 검출되었다. 대형마트와 재래시장에서 수거한 고춧가루의 일반세균수 검출량은 통계적으로 유의적인 차이는 없었으나 7 log CFU/g을 초과하는 고춧가루의 수는 대형마트보다 재래시장이 더 많았다. 보관온도(30℃, 4℃, -20℃)와 보관용기(지퍼백, 비닐봉투)에 따라 7개월까지 저장 실험한 결과, 저장 기간에 따라 미생물학적 품질에는 큰 차이가 나타나지 않았다. 그러나 30℃에 저장한 고춧가루의 수분함량 및 ASTA color value는 저장 3개월 이후 크게 감소하였다. 따라서 고춧가루의 장기 보관 시 품질을 유지하기 위해서는 냉장고 또는 냉동고에 보관하는 것이 바람직하다고 생각된다.

• 환경생물
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• 제22권4호
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• pp.494-500
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• 2004

In order to investigate the effect on morphology of Rhizopus oryzae and production of lactic acid, various interface materials were used. Morphology of fungal showed sheet and flock when resin was added. The production of lactic acid was increased dramatically when interface materials were added. Furthermore, the effect of resin was more significant than that of others. It was assumed that interface materials could absorb substrate and microorganism together, so microorganism was not inhibited by substrate. The effect of static electric field on the interface culture was studied. When the exerting potential was 6.78 voltage, the biomass y was obviously higher than that of zero voltage. A simulated gas-solid interface system was developed to study the growth and two phases model for the growth of Rhizopus oryzae was build up that depended on the symmetric branching theory. An important parameter F was researched. The results indicated that the value of F had obvious difference at exponential and deceleration period, respectively.

• KSBB Journal
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• 제6권1호
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• pp.85-90
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• 1991

Experiments of alcohol fermentation of the yeast,K. fragi1is CBS 1555 were performed to obtain the following results. In these experiments, the initial concentrations of sugar which was composed of inulin and fructose as weight ratio of one to one were 30, 50, 75, 100 and 150g/l and the initial densities of the microorganism were less than 0.5g/l, 10g/1 to 15g/1, and 50g/l. The functional relationship among specific growthrates, sugar concentrations, and alcohol concentrations could be expressed by Aiba-shoda equation and the specific growth rate represented the trend that decreased with increase in the initial concentration of the microorganism. Also, $\mu$max and Ks of Monod's equation could be expressed as the function of initial cell concentration like the following equations. $\mu$max=0.8-0.008X Ks=0.54X+8 In the region that sugar, alcohol and cell concentrations were 10g/1 to 120g/l, 0g/l to 60g/l and 0.5g/l to 50g/l respectively, the differences between the experimental values and the calculated ones for specific growth rate approached to 40% with respect to experimental values at the worst cases, but in most cases, those were distributed in the range of less than 20%.