• Title/Summary/Keyword: microorganism ecosystem

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Analysis of Microbial Community Structure in River Ecosystem Using Quinone Profiles (Quinone profile를 이용한 하천생태계의 미생물군집구조 해석)

  • Lim, Byung-Ran;Lee, Kisay;Ahn, Kyu-Hong
    • Journal of Korean Society of Water and Wastewater
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    • v.20 no.5
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    • pp.685-690
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    • 2006
  • The differences in microbial community structures between planktonic microorganism and biofilm in rivers were investigated using respiratory quinone profiles. The compositions of microbial quinone for 4 tributaries of the Kyongan Stream located in/flowing through Yongin City, Gyeonggi-Do were analyzed. Ubiquinone(UQ)-8, UQ-9, menaquinone(MK)-6 and Plastoquinone(PQ)-9 were observed in all samples of planktonic microorganism and biofilm for the sites investigated, Most planktonic microorganism and biofilm had UQ-8(15 to 30%) and PQ-9(over 30%) as the dominant quinone type. These results indicated that oxygenic phototrophic microbes(cyanobacteria and/or eukaryotic phytoplankton) and UQ-8 containing proteobacteria constituted major microbial populations in the river. The quinone concentration in the river waters tested, which reflects the concentration of planktonic microorganisms, increases with increasing DOC. Further research into this is required. The microbial diversities of planktonic microorganism and biofilm calculated based on the composition of all quinones were in the range from 4.2 to 7.5, which was lower than those for activated sludge(ranging from 11 to 14.8) and soils(ranging from 13.4 to 16.8). The use of quinone profile appears to be a useful tool for the analysis of microbial community structure in river.

Influences of New Azo Dyes to the Aquatic Ecosystem

  • Bae Jin-Seok;Freeman Harold S.;Kim Sung-Dong
    • Fibers and Polymers
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    • v.7 no.1
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    • pp.30-35
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    • 2006
  • The influences of a series of new azo direct dyes including copper-complexes based on benzidine congeners, 2,2'-dimethyl-5,5'-dipropoxybenzidine and 5,5'-dipropoxybenzidine, were examined using microorganism, Daphnia magna. The purpose of the research described in this paper was to use bioassays with daphnids to determine the aquatic toxicity of new azo dyes in which copper was incorporated. The results clearly show that copper has negative effects to aquatic ecosystem as expected. The study also suggested that the assay with Daphnia magna was an excellent method to evaluate the influences of dyes to the aquatic environment.

Temporal and Spatial Change in Microbial Diversity in New-developed Wetland Soil Covered by Tamarix chinesis Community in Chinese Yellow River Delta

  • Chen Weifeng;Ann Seoung-Won;Kim Hong-Nam;Shi Yanxi;Mi Qinghua
    • Journal of Environmental Science International
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    • v.14 no.4
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    • pp.367-371
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    • 2005
  • Soil samples were collected from new-developed wetland soil ecosystem of Tamarix chinesis plantation in Chinese Yellow River Delta in different months of 2003. Soil characteristics, temporal change and spatial distribution of microbial community composition and their relationship with nitrogen turnover and circling were investigated in order to analyze and characterize the role of microbial diversity and functioning in the specific soil ecosystem. The result showed that the total population of microbial community in the studied soil was considerably low, compared with common natural ecosystem. The amount of microorganism followed as the order: bacteria> actinomycetes>fungi. Amount of actinomycetes were higher by far than that of fungi. Microbial population remarkably varied in different months. Microbial population of three species in top horizon was corrected to that in deep horizon. Obvious rhizosphere effect was observed and microbial population was significantly higher in rhizosphere than other soils due to vegetation growth, root exudation, and cumulative dead fine roots. Our results demonstrate that microbial diversity is low, while is dominated by specific community in the wetland ecosystem of Tamarix chinesi.

Environmental toxicity and biodegradation of Pseudomonas sp. EL-G527 producing biosurfactant

  • Mi Seon, Cha;Hong Ju, Son;Sang Jun, Lee
    • Proceedings of the Korean Environmental Sciences Society Conference
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    • 2002.05b
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    • pp.452-454
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    • 2002
  • A biosurfactant-producing microorganism, .Pseudomouos sp. EL-G527 was isolated from activated sludge by enrichment culture when grown on mineral salt medium containing n-hexadecane as a carbon source. The biosurfactant from .Pseudomonar sp. EL-G527 exhibited lesser toxicity to bacterial population than synthetic surfactants and in the biodegradation test, biosurfactant was rapidly degraded and lost its activity as surface active material after 1 day incubation. In this study, the biosurfactant from Pseudomonas sp. EL-G527 was effective surface-active compound, more biodegradable and less toxic to microbial ecosystem than various synthetic surfactants.

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Prospects of Activated Sludge Process in Japan - Its Past, Present, and Future -

  • Fujita, Masanori
    • Journal of Wetlands Research
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    • v.9 no.1
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    • pp.61-67
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    • 2007
  • Our life totally depends on activated sludgeprocess for treatment of wastewater: sewage and industrial wastewater. Activated sludge process was the epoch-making technology in Environmental field. One century has been almost passed since the process was developed in England, and the process is still on the development of improvement. Here, history of activated sludge process, its mechanismsof treating the wastewater, expectations that we had on the process in the past, and future image and possibility on the process were presented. By reviewing the events related to the process, we can foresee potentials for new possibility of activated sludge process.

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Fate and Activity of Microorganism introduced into the Soil (토양에 투입된 미생물의 거동 및 활성)

  • Chung, Jae-Chun;Ju, Seul;Lee, Jae-Woong;Lee, Jung-Jae
    • Journal of the Korea Organic Resources Recycling Association
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    • v.10 no.2
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    • pp.100-116
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    • 2002
  • There are several purpose to introduce microorganism into the Soil. The major purpose is to promote plant growth and inhibit plant pathogens. The model example is to put in nitrogen fixing symbiotic bacteria, Pythium and Rhizobium. In order to achieve the intended goal, the introduced microorganism should survive and colonize with sufficient density. The survival of introduced microorganism depend upon biotic and abiotic factors. Predation and competition are important among biotic factor. Water tension, organic carbon, inorganic nutrients(N, P), pH are important factor among abiootic factor. Soil texture and distribution of soil pore are also important in the survival and colonization of introduced microorganism. Selection by soil ecosystem for inoculant is a crucial factor for colonization. Good example are control of autochtonous microorganism and the introduction of surfactant biodegrading Pseudomonas. Sometimes, carriers such as peat and montmorillonite can be added to help colonization. Carriers can protect introduced microorganism by supplying protective microhabitat. Organic polymer is also used as a carrier to immobilize bacteria or industrial enzymes. Examples of these carrier are calcium alginate, agarose and k-carrageenan. The function of these carrier is to provide microhabitat and help colonization for introduced microorganism.

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Effect of Microorganism Mixture Application on the Microflora and the Chemical Properties of Soil and the Growth of Vegetables in Greenhouse (미생물혼합제제 처리가 토양의 미생물상과 화학적 특성 및 시설 채소 생육에 미치는 영향)

  • Ryu, Il-Hwan;Jeong, Su-Ji;Han, Seong-Soo
    • Korean Journal of Environmental Agriculture
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    • v.31 no.4
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    • pp.368-374
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    • 2012
  • BACKGROUND: The urgency of feeding the world's growing population while combating soil pollution, salinization and desertification requires suitable biotechnology not only to improve crop productivity but also to improve soil health through interactions of soil nutrient and soil microorganism. Interest in the utilization of microbial fertilizer has increased. A principle of nature farming is to produce abundant and healthy crops without using chemical fertilizer and pesticides, and without interrupting the natural ecosystem. Beneficial microorganisms may provide supplemental nutrients in the soil, promote crop growth, and enhance plant resistance against pathogenic microorganisms. We mixed beneficial microorganisms such as Bacillus sp. Han-5 with anti-fungal activities, Trichoderma harziaum, Trichoderma longibrachiatum with organic material degrading activity, Actinomycetes bovis with antibiotic production and Pseudomonas sp. with nitrogen fixation. This study was carried out to investigate the mixtures on the soil microflora and soil chemical properties and the effect on the growth of lettuce and cucumber under greenhouse conditions. METHODS AND RESULTS: The microbial mixtures were used with each of organic fertilizer, swine manure and organic+swine manure and compared in regard to changes in soil chemical properties, soil microflora properties and crop growth. At 50 days after the treatment of microorganism mixtures, the pH improved from 5.8 to 6.3, and the EC, $NO_3$-Na and K decreased by 52.4%, 60.5% and 29.3%, respectively. The available $P_2O_5$ and $SiO_2$ increased by 25.9% and 21.2%, respectively. Otherwise, the population density of fluorescent Pseudomonas sp. was accelerated and the growth of vegetables increased. Moreover, the population density of E. coli and Fusarium sp., decreased remarkably. The ratio of bacteria to fungi (B/F) and the ratio of Actinomycetes bovis to fungi (A/F) increased 2.3 (from 272.2 to 624.4) and 1.7 times (from 38.3 to 64), respectively. Furthermore, the growth and yield of cucumber and lettuce significantly increased by the treatment of microorganism mixtures. CONCLUSION(S): These results suggest that the treatment of microorganism mixtures improved the chemical properties and the microflora of soil and the crop growth. Therefore, it is concluded that the microorganism mixtures could be good alternative soil amendments to restore soil nutrients and soil microflora.

Energy Flow in a Coastal Salt Marsh Ecosystem (海岸 鹽濕地 生態系의 에너지 流轉)

  • Kim, Joon-Ho;Beung Tae Ryu
    • The Korean Journal of Ecology
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    • v.8 no.3
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    • pp.153-161
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    • 1985
  • Energy flow through the trophic levels was studied at a salt marsh ecosystem distinguished into low and high marsh. Gross primary productions of Suaedeto-Salicornietum and Artemisieto-Limonietum at low marsh were 8, 299 and 13, 154kca/$m^2$/yr, and those of Calama-grostetum and Sonchuso-Setaetum at high marsh were 17, 899 and 15, 177kca/$m^2$/yr, respectively. Efficiencies of solar energy utilization of plants were 1.7 and 2.6% at the former, and were 3.6 and 3.2% at the latter. Of gross productions, net primary productions were 3, 977 and 5, 280kca/m2/yr at low marsh and were 6, 354 and 5, 329kca/$m^2$/yr at high marsh, and the remainder, 52~67%, was consumed by respiration of plants. A small amount (0.03~0.04%) of the net primary production was flowed through grazing food chain and most amout was transferred into dead parts. Of dead parts, 40% was accuulated as litter and the rest was decomposed into detritus. In the detritus food chain, a little energy was utilized by detritus feeder, and a major by microorganism. The amounts of energy flowed through grazing and detritus feeders at high marsh were much more than those at low marsh, but tertary production as spider was Vice versa.

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Sutdy of Appropriate Media Selection and Early Life Cycle of Marine Free-Living Nematodes, Enoplolaimus sp. (Enoplida: Thoracostomopsidae) and Bathylaimus sp. (Enoplida: Tripyloididae) (해양 자유생활형 선충류 Enoplolaimus sp. (Enoplida: Thoracostomopsidae)와 Bathylaimus sp. (Enoplida: Tripyloididae)의 배양용 적합배지 선정 및 초기 생활사 연구)

  • SHIN, AYOUNG;KIM, DONGSUNG;KANG, TEAWOOK;OH, JE HYEOK;LEE, JIMIN;HONG, JAE-SANG
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.23 no.3
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    • pp.109-124
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    • 2018
  • In order to find the optimum culture condition for marine free-living nematoda in the laboratory, various agar media were developed and experiments were carried on nematodes. Nematodes have collected from the bottom of the sandy sediments' surface layer (about 5cm sediment) of Taean Mallipo beach's intertidal zone. Especially, with regard to agar medium, Killian medium was transformed slightly, density of agar had made a difference and this agar medium(height 2.0 mm on diameter 60 mm Petri dish) was divided. It was mixed with 5 different species of microorganism as nematodes' live food and added each culture medium. Five individuals of Enoplolaimus sp. on each culture medium were grown in a culture medium which was set on $20^{\circ}C$ and light blocked. Moreover, as a result of the optimum culture condition, 1.0% density of agar showed the highest survival rates (the average time of survival is 246.8 hours). On the other hand, the 0.4% density of agar adding Killian medium, bacto peptone and beef extract showed the lowest survival rates, which indicates the average time of survival is 27.6 hours. About Bathylaimus sp., on Killian medium's 1.0% density of agar(no feeding amount) showed the highest survival rates, which connects that the hatching rate 94.7% after 99.5 hours and it laid 7 eggs averagely on the spawning amount and the hatching rate experiment.

The Restoration of Forest Fire Area in Kagawa Prefecture, Japan (일본 가가와현 산불피해지의 복구대책)

  • Chun, Kun-Woo;Lee, Si-Young;Lim, Young-Hyup;Kakihara, Toshiko;Ezaki, Tsugio
    • 한국방재학회:학술대회논문집
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    • 2007.02a
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    • pp.238-241
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    • 2007
  • The forest seemed apparently to die on the forest fire area in Honjima, Kagawa Prefecture, Japan. However, the soil that became growing basic of vegetation hardly suffered damage, and the forest recovery was started by the sprout, etc. in the next year. For restoration of forest fire area, the fascine mulching works and log barrier works using the damaged trees were used for the upper-stream, and chack dam and erosion control dam were set up in the downstream. Also, the forest restoration was tried with the plants and the microorganism that inhabit in Honjima to preserve a peculiar forest ecosystem.

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