Browse > Article
http://dx.doi.org/10.5338/KJEA.2012.31.4.368

Effect of Microorganism Mixture Application on the Microflora and the Chemical Properties of Soil and the Growth of Vegetables in Greenhouse  

Ryu, Il-Hwan (College of Life Science and Natural Resources, Wonkwang University)
Jeong, Su-Ji (College of Life Science and Natural Resources, Wonkwang University)
Han, Seong-Soo (College of Life Science and Natural Resources, Wonkwang University)
Publication Information
Korean Journal of Environmental Agriculture / v.31, no.4, 2012 , pp. 368-374 More about this Journal
Abstract
BACKGROUND: The urgency of feeding the world's growing population while combating soil pollution, salinization and desertification requires suitable biotechnology not only to improve crop productivity but also to improve soil health through interactions of soil nutrient and soil microorganism. Interest in the utilization of microbial fertilizer has increased. A principle of nature farming is to produce abundant and healthy crops without using chemical fertilizer and pesticides, and without interrupting the natural ecosystem. Beneficial microorganisms may provide supplemental nutrients in the soil, promote crop growth, and enhance plant resistance against pathogenic microorganisms. We mixed beneficial microorganisms such as Bacillus sp. Han-5 with anti-fungal activities, Trichoderma harziaum, Trichoderma longibrachiatum with organic material degrading activity, Actinomycetes bovis with antibiotic production and Pseudomonas sp. with nitrogen fixation. This study was carried out to investigate the mixtures on the soil microflora and soil chemical properties and the effect on the growth of lettuce and cucumber under greenhouse conditions. METHODS AND RESULTS: The microbial mixtures were used with each of organic fertilizer, swine manure and organic+swine manure and compared in regard to changes in soil chemical properties, soil microflora properties and crop growth. At 50 days after the treatment of microorganism mixtures, the pH improved from 5.8 to 6.3, and the EC, $NO_3$-Na and K decreased by 52.4%, 60.5% and 29.3%, respectively. The available $P_2O_5$ and $SiO_2$ increased by 25.9% and 21.2%, respectively. Otherwise, the population density of fluorescent Pseudomonas sp. was accelerated and the growth of vegetables increased. Moreover, the population density of E. coli and Fusarium sp., decreased remarkably. The ratio of bacteria to fungi (B/F) and the ratio of Actinomycetes bovis to fungi (A/F) increased 2.3 (from 272.2 to 624.4) and 1.7 times (from 38.3 to 64), respectively. Furthermore, the growth and yield of cucumber and lettuce significantly increased by the treatment of microorganism mixtures. CONCLUSION(S): These results suggest that the treatment of microorganism mixtures improved the chemical properties and the microflora of soil and the crop growth. Therefore, it is concluded that the microorganism mixtures could be good alternative soil amendments to restore soil nutrients and soil microflora.
Keywords
Growth of vegetables; Soil chemical properties; Soil microflora; Treatment of microorganism mixtures;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Federico, G.R., Maria, M.R., Marcela, F., Sofia, N.C., Adriana, M.T., 2007. Biological control by Trichoderma species of Fusarium solani causing peanut brown root rot under field conditions, Crop Protect, 26, 549-555.   DOI   ScienceOn
2 Glick, B.R., Karaturovic, D.M., Newell, P.C., 1995. A novel procedure for rapid isolation of plant growth promoting Pseudomonas, Can. J. Microbiol. 41, 533-536.   DOI   ScienceOn
3 Ha, H.S., Um, S.K., Kang, H., Park, J.C., 1979. Chemical properties of the soils under plastic film house in the southern forcing culture areas, Horticul Environ. Biotechnol. 20, 36-46.
4 Johnson, D.L., Anderson, D.R., McGrath, S. 2005. Soil microbial response during the phytoremediation of a PAH contaminated soil, Soil Biol. Biochem. 37, 1334-1336.
5 Kennedy, I.R., Pereg-Gerk, L.L., Wood, C., Deaker, R., Gilchrist, K., Katupitiya, S., 1997. Biological nitrogen fixation in non-leguminous field crop: facilitating the evolution of an effective association between Azospirillium and wheat, Plant Soil 194, 65-79.   DOI   ScienceOn
6 Kim, J.Y., Cho, K.S., 2006. Bioremediation of oilcontaminated soil using rhizobacteria and plant, Kor. J. Microbiol. Biotechnol. 34, 185-195.
7 Li, J., Kremer, R.J. 2006. Growth response of weed and crop seeding to deleterious rhizobacteria, Biol. Control, 39, 58-65.   DOI   ScienceOn
8 Thomas, F.C., Chin, A.W., Priester, W., Bij, A.J., Lugtenberg, J.J., 1997. Description of the colonization of a gnotobiotic tomato rhizosphere by Pseudomonas fluorescens biocontrol strain WCS365 using scanning electron microscopy, Mol. Plant Microbe. 10, 79-86.   DOI   ScienceOn
9 Kloepper, J.W., Gutierrwz-estrada, A., Melnroy, J.A., 2007. Photoperiod regulates elicitation of growth promotion but not induced resistance by plant growth-promoting rhizobacteria, Can. J. Microbiol. 53, 159-167.   DOI   ScienceOn
10 Kwon, J.S., Suh, J.S., Weon, H.Y., Shin, J.S., 1998. Evalution of soil microflora in salt accumulated soils of plastic film house, Korean J. Soil and Fertilizer, 31, 204-210.
11 Ahn, T.S., Ka, J.O., Lee, G.H., Song, H.G., 2007. Revegetation of a lakeside barren area by the application of plant growth-promoting rhizobacteria, J. Microbiol. 45, 171-174.
12 Liste, H., Felgentreu, D., 2006. Crop growth, culturable bacteria, and degradation of petrol dehydrocarbons (PHCs) in a long-term contaminated field soil, Appl. Soil Ecol. 31, 43-52.   DOI   ScienceOn
13 Macek, T.M. and Kas, J. 2000. Exploitation of plants for the removal of organics in environmental remediation, Biotechnol. Adv. 18, 23-34.   DOI   ScienceOn
14 Meers, E., Vandecasteele, B., Ryttens, A., Vangronsveld, J., Track, F.M.G., 2007. Potential of five willow species(Salix spp.) for phytoextraction of heavy metal, Environ. Experim. Bota. 60, 57-68.   DOI   ScienceOn
15 NIAST. 1988. Methods of soil chemical analysis. National Institute of Agricultural Science and Technology, RDA, Suwon, Korea.
16 Nehl, D.B., Allen, S.J., Brown, J.F., 1996. Deleterious rhizosphere bacteria, an intergrating perspective, Appl. Soil Ecol. 5, 1-20.
17 Riddle, J., Ford, J., 1995. Organic Inspection Manual Independent Organic Inspectors Association, p. 121, Minnesota, USA.
18 Schnoor, J. L. 1997. Phytoremediation technology evaluation report, Ground-Water Remediation Technologies Analysis Center, pp.8-18, Iowa, U.S.A.
19 Shaharoona, B., Arshad, M., Khilid, A., 2007. Differential response of etiolated pea seedlings to inoculation with rhizobacteria capable of utilizing 1-aminocyclopropane-1-carboxylate or L-methionine, J. Microbiol. 45, 15-20.
20 Shimp, J.F., Tracy, J.C., Davis, L.C., Lee, E., Huang, W., Erickson, L.E., Schnoor, J.L. 1993. Beneficial effect of plants in the remediation of soil and groundwater contaminated with organic meterials, Environ. Sci. Technol. 23, 41-77.   DOI   ScienceOn
21 Estiken, A. Pirlak, L., Turan, M., Sahin, F., 2006. Effects of floral and foliar application of plant growth promoting rhizobacteria(PGPR) on yield, growth and nutrition of sweet cherry, Sci. Horticamsterdam 110, 324-327.   DOI   ScienceOn
22 Aslantas, R., Ramazan, C. and Sahin, F., 2007. Effect of plant growth promoting rhizobacteria on young apple tree growth and fruit yield under orchard condition, Sci. Horticamsterdam 111, 371-377.   DOI   ScienceOn
23 Bray, R.H. and Kurtz, L.T., 1945. Determination of total, organic and available forms of phosphorus in soil, Soil Sci. 59, 39-45.   DOI
24 Cunningham, S.D. and Berti, W.R. 1993. Remediation of contaminated soils with green plants: an overview, InVitro Cell Dev. Biol. 29, 207-220.   DOI