• Journal of the Society of Cosmetic Scientists of Korea
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• v.50 no.2
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• pp.119-129
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• 2024

The cutaneous lymphatic system in humans plays a crucial role in draining interstitial fluid and activating the immune system. Environmental factors, such as ultraviolet light and natural aging, often affect structural changes of such lymphatic vessels, causing skin dysfunction. However, some limitations still exist because of no alternatives to animal testing. To better understand the skin lymphatic system, a biomimetic microfluidic platform, skin-lymph-on-a-chip, was fabricated to develop a novel in vitro skin lymphatic model of humans and to investigate the molecular and physiological changes involved in lymphangiogenesis, the formation of lymphatic vessels. Briefly, the platform involved co-culturing differentiated primary normal human epidermal keratinocytes (NHEKs) and dermal lymphatic endothelial cells (HDLECs) in vitro. Based on our system, Lymphanax^TM, which is a condensed Panax ginseng root extract obtained through thermal conversion for 21 days, was applied to evaluate the lymphangiogenic effect, and the changes in molecular factors were analyzed using a deep-learning-based algorithm. Lymphanax^TM promoted healthy lymphangiogenesis in skin-lymphon-a-chip and indirectly affected HDELCs as its components rarely penetrated differentiated NHEKs in the chip. Overall, this study provides a new perspective on Lymphanax^TM and its effects using an innovative in vitro system.

• Proceedings of the KSME Conference
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• 2007.05b
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• pp.2781-2784
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• 2007

Early detection of a disease is important to tackle treatment issues in a better manner. Several diagnostic techniques are in use, these days; for such purpose and tremendous research is going on to develop newer and newer methods. However, more work is required to be done to develop cheap and reliable early detection techniques. Micro-fluidic chips are also playing key role to deliver new devices for better health care. The present study focuses on a review of recent developments in the interrogation of different techniques and present state-of-the-art of microfluidic sensor for better, quick, easy, rapid, early, inexpensive and portable POCT (Point of Care testing device) device for a particular study, in this case, bone disease called osteoporosis. Some simulations of the microchip are also made to enable feasibility of the development of a blood-chip-based system. The proposed device will assist in early detection of diseases in an effective and successful manner.

• Proceedings of the KIEE Conference
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• 2004.07c
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• pp.2099-2101
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• 2004

본 연구에서 제안하는 마이크로 시스템은 열공압 방식으로 구동되고 제작비용이 저렴한 indium tin oxide (ITO) 및 polydimethylsiloxane (PDMS)로 제작되었다. 제안된 마이크로 밸브와 마이크로 펌프의 구조는 ITO 히터, SU-8 층, PDMS membrane, 그리고 PDMS 채널로 구성 되어 있다. 제안된 마이크로 펌프와 마이크로 밸브는 제작 공정 및 구조가 간단하고 값이 저렴하며, 마이크로 펌프와 마이크로 밸브를 같은 기판 위에 쉽게 직접화할 수 있는 장점을 가진다. 마이크로 밸브의 유량은 채널 폭에 비례하며 밸브가 closing 되는 전력은 채널의 폭과 상관없이 100 mW이다. 마이크로밸브의 ITO 히터의 온-오프에 따라 유량이 매우 잘 제어되었다. 제안된 마이크로 펌프의 경우, 히터의 인가 펄스 전압이 증가함에 따라 유량은 선형적으로 비례 증가함을 관찰할 수 있다. 마이크로 펌프의 최대 유량은 펄스 전압과 duty 비가 55V와 10%일 때 6 Hz에서 78 nl/min이 측정 되었다.

• Proceedings of the KIEE Conference
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• 2011.07a
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• pp.1686-1687
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• 2011

A microfabricated chip with in-channel electrochemical cell using interdigitated gold electrode was fabricated for sensitive electrochemical analysis. The gold electrodes were fabricated on glass wafer using thermal evaporator and were covered using PDMS mold containing microchannel for analyte and electrolyte. The active area of each electrode was $250\;{\mu}m{\times}200\;{\mu}m$ with a gap of 200 ${\mu}m$ between the electrodes. Microelectrodes results in maximum amplification of signal, since the signal enhancement effect due to cycling of the reduced and oxidized species strongly depends on the inter electrode distance. Analytes such as methylene blue and guanosine were characterized using the fabricated electrodes and their electrochemical characteristics were compared with conventional bulk electrodes. The device so developed shall find use as disposable electrochemical cell for rapid and sensitive analysis of electroactive species.

• Journal of the Korean Society of Visualization
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• v.1 no.2
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• pp.47-51
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• 2003

Microfluidic chips such as lab-on-a-chip (LOC) include micro-channels for sample delivery, mixing, reaction, and separation. Pressure driven flow or electro-osmotic flow (EOF) has been usually employed to deliver bio-samples. Having some advantages of easy control, the flow characteristics of EOF in microchannels should be fully understood to effectively control the electro-osmotic pump for bio-sam-pie delivery. In this study, a micro PIV system with an epifluorescence inverted microscope and a cooled CCD was used to measure velocity fields of EOF in a glass microchannel and a PDMS microchannel. The EOF velocity fields were changed with respect to electric charge of seeding particles and microchannel materials used. The EOF has nearly uniform velocity distribution inside the microchannel when pressure gradient effect is negligible. The mean streamwise velocity is nearly proportional to the applied electric field. Glass microchannels give better repeatability in PIV results, compared with PDMS microchannels which are easy to fabricate and more suitable for PIV experiments.

• Proceedings of the KIEE Conference
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• 2009.07a
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• pp.1521_1522
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• 2009

Chemotaxis is the directed movement of cells in gradients of signaling molecules, an essential biological process that underlies morhpogenesis during development, and the recruitment of immune cells to sites of infection. Especially, bacterial chemotaxis has utilized as an important prelude to study metabolism, prey-predator relationship, symbiosis, other ecological interactions in microbial communities. Recently, novel analytical formats integrated with microfluidics were introduced to investigate the chemotaxis of the cells with the precise control of chemical gradient and small volume of cells. In this study, we present a method to detect bacterial chemotaxis by direct fluidic contacting. The developed fluidic-handling method is driven by capillary force, hydrophobic barrier and a cohesion force between fluids. We have investigated the chemotactic response of E Coli. and Pseudomonas aeruginosa to three kinds of chemoeffectors such as HEPES buffer, peptone and chloroform.

• Current Optics and Photonics
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• v.4 no.1
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• pp.44-49
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• 2020

In this paper, a new method is presented to measure the refractive index of single plain glass or multilayered materials, based on a laser frequency-shifted confocal feedback microscope. Combining the laser frequency-shifted feedback technique and the confocal effect, the method can attain high axial-positioning accuracy, stability and sensitivity. Measurements of different samples are given, including N-BK7 glass, Silica plain glass, and a microfluidic chip with four layers. The results for N-BK7 glass and Silica plain glass show that the measurement uncertainty in the refractive index is better than 0.001. Meanwhile, the feasibility of this method for multilayered materials is tested. Compared to conventional methods, this system is more compact and has less difficulty in sample processing, and thus is promising for applications in the area of refractive-index measurement.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.31 no.4
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• pp.392-397
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• 2007

We developed a microfluidic platform able to control the trap and release of magnetic beads used for separation of a specific biomolecules. The magnetic beads can be trapped and released conditionally by controlling the difference between the Stokes force induced by the fluid flow and magnetic force resulting from a permanent magnet. The permanent magnet of CoNiP alloy is electroplated. It is characterized to have the 1369 Oe of coercivity, 1762 Gauss of remanence, and 0.603MGOe of (BH)max. Through the experimental and numerical investigation, the magnetic beads are trapped under the flow velocity of 17 ${\mu}m/s$ and are released perfectly above the velocity of 174 ${\mu}m/s$.

• Proceedings of the Korean Vacuum Society Conference
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• 2014.02a
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• pp.209.2-209.2
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• 2014

In recent years, researches about hydrophobic and hydrophilic surfaces have been executed however their other effects have not been researched enough. In this paper, the fabrication method of hierarchical structures of micro line array combined with fluorinated wax for anisotropic superomniphobic wettability is presented. We have achieved anisotropic and superomniphobic surface via simple two step methods, which are maskless photolithography and wax deposition. In order to prove how to provide those characteristics, SEM, contact angle measurement tool and X-ray diffraction are used. Fluorinated wax is crystalized self-assembly and it is subordinated on micro line array so that it is able to display anisotropic wettability. Understanding on anisotropic superomniphobic surface and simple fabrication method has been attracted to apply for lots of applications which range from self-cleaning surface, microfluidic chip, to directionally fluid control device, even in oily fluid.

• 유체기계공업학회:학술대회논문집
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• 2005.12a
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• pp.89-93
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• 2005

In this work, perfusive particles are used to form a micro column in a microfluidic chip and flow properties of the micro column are investigated. The packing flow velocity and the column/particle size ratio are shown to be important parameters affecting the packing density of the micro column. Experimental results show that the effect of the column/particle size ratio on the flow resistance of the micro column is negligible. This contrasts with previous works on the effect of the column/particle size ratio on the total pressure drop across the column.