• Proceedings of KOSOMES biannual meeting
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• 2005.11a
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• pp.125-132
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• 2005

Marine Technology is the last hope of mankind for in resolving predestined issues of food, resources, and environmental problems. Facing and realizing this great challenge, domestic industrial, academic and research institutional experts participated in formulating the Marine Technology Development Plan, and the national Science and Technology Committee chaired by the president deliberated and passed this important plan thus setting the foundation for activating and promoting MT development. A project. to develop Marine Technology Road Map(MTRM) is being carried out since June of 2005 for effective undertaking of such endeavor. This paper investigate current issues in MY, MTRM status, establishing and operating MT R&D Expert Institutes, funding resources, etc to provide recommendations for promoting and stimulating MT.

• Korean Journal of Crystallography
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• v.2 no.2
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• pp.28-31
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• 1991

4-크로로-4'-메톡시-2-니트로디페닐아민, (C12H11N2O3CL, FW=278.70)의 단것세포 상수는 a=8,169(3), b=8.883(1), c=9.150(1) h, α =82. 98(1), β=104.80(2), y=101.43(2)", V=627.3 A3, F(000)=288.0, Dc=1.48g/cm3, u=3.06cm-1, 7=295" K, 공간군 Pi, 번호 2, 삼사 정계 이며, Z=2이다. λ (Mo-Ka)=0.7107A을 사용 하여 수집한 독립적인 회절 반점 1541개로 구조분 석한 최종 신뢰도 값은 각각 R=0.032, Rw=0.033 이며, S=0.46이다. 본 화합물은 암모니아의 두개 의 수소 대신에 4-크로로-페닐기와 4-메톡시-페닐기로 치환된 물질로써, 질소와 두 페닐기 사이의 각과 결합거리 들은 각각 125.42", 1.362 및 1.428 A 인바 수소와 함께 SP2_혼성결합을 하고있다. 질소를 포함한 두 면간 각은 63.29"이다. 분자간 어 떠 한 수소결합을 하고 지지 않다.

• Korean Journal of Pharmacognosy
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• v.48 no.2
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• pp.125-133
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• 2017

In this study, we investigated the total phenolic and flavonoid contents, component analysis, antioxidative activity and cellular protective effects against oxidative stress on human skin cells in 50% ethanol extract, ethyl acetate fraction and aglycone fraction obtained from Gynostemma pentaphyllum (G. pentaphyllum) Makino. The DPPH (1,1-diphenyl-2-picrylhydrazyl) scavenging activites ($FSC_{50}$) of the 50% ethanol extracts, ethyl acetate fraction and aglycone fraction were 246.8, 147.2, $128.9{\mu}g/mL$, respectively. Reactive oxygen species (ROS) scavenging activities ($OSC_{50}$) of the 50% ethanol extract, ethyl acetate fraction and aglycone fraction on ROS generated in $Fe^{3+}-EDTA/H_2O_2$ system using the luminol-dependent chemiluminescence assay were 37.15, 10.74, $7.19{\mu}g/mL$, respectively. We investigated the cellular protective activity and the results showed that treatment of aglycone fraction ($0.05-0.39{\mu}g/mL$) protect human skin cells in a concentration-dependent manner when the skin cell damages were induced by treating them with $H_2O_2$. These results suggest that extract/fractions of G. pentaphyllum Makino may be applicable as natural antioxidants in cosmetics.

• Applied Biological Chemistry
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• v.49 no.2
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• pp.125-128
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• 2006

Schizonepeta tenuifolia Briq. are used in folk medicine as common cold, sore throat, headache and skin infection in Korea, China and Japan. To compare the contents of domestic and Chinese Schizonepeta tenuifolia quantitative HPLC analysis was performed using a Luna $C_{18}$ column $(4.6{\times}250\;mm,\;5{\mu}m)$, with 1 ml/min of flow rate of 0.01% formic acid in water : acetonitrile = 50 : 50 under UV 254 nm of detector. Pulegone was detected at retention time of about 17.14 min.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.33 no.2
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• pp.103-109
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• 2000

Complete senuences of the mitochondrial rRNA Benes were determined among six salmonines in Korean Waters (Brachpmystax lenok, Onoorhpchus keta, O. masou mason, O. mason ishikawae, O. mykiss, and albino mutant of O. mykiss). The purposes of this study were to provide the basic information on levels of mtDNA polymorphism among these species for genetic characterization; discuss phylogentic relationships among three Oncorhynchus sepecies; demonstrate the utility of rRNA gene sequence data as a genetic marker for disringuishinf among Korean salmonines. PCR/direct sequencing data indicated the following consistent results; 1) 12S rRNA genes was 945 bases long in Oncorhynchus species, and 946 bases in B. lenot including one insertion. 2) Of sequence variation in mitochondrial rRNA regions, transitional substitutions were superior to transversion. 3) The significant differences were not shown in the intraspecific variation values in these gene regions. The percentage sequence divergence values were ranged from $0.066 to 0.212{\%}$. 4) The interspecific divergences were greater than the intraspecific variation. Nevertheless, ribosomal RMh genes were more conserved among species than the other mitochondrial genes, and they showed potentiality as an intergenic marker for systematics. In addition, phylogenetic trees, constructed from this data, supported that cherry salmon was closer to chum salmon than to rainbow trout, and that lenok was most distantly related species in six salmonid species.

• Genomics & Informatics
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• v.17 no.4
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• pp.48.1-48.6
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• 2019

Over the last decade, genome-wide association studies (GWASs) have provided an unprecedented amount of genetic variations that are associated with various phenotypes. However, previous GWAS were mostly conducted in European populations, and these biased results for non-Europeans may result in a significant reduction in risk prediction for non-Europeans. An issue with the early GWAS was the winner's curse problem, which led to misleading results when constructing the polygenic risk scores (PRS). Therefore, more non-European population-based studies are needed to validate reported variants and improve genetic risk assessment across diverse populations. In this study, we validated 422 variants independently associated with glycemic indexes, liver enzymes, and type 2 diabetes in 125,872 samples from a Korean population, and further validated the results by assessing publicly available summary statistics from European GWAS (n = 898,130). Among the 422 independently associated variants, 284, 320, and 361 variants were replicated in Koreans, Europeans, and either one of the two populations. In addition, the effect sizes for Koreans and Europeans were moderately correlated (r = 0.33-0.68). However, 61 variants were not replicated in both Koreans and Europeans. Our findings provide valuable information on effect sizes and statistical significance, which is essential to improve the assessment of disease risk using PRS analysis.

• Journal of Korean Academic Society of Home Health Care Nursing
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• v.22 no.2
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• pp.246-255
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• 2015

Purpose: The study was conducted to develop the Nursing Practice Capacity scale for evaluating achievement of education objectives among students in nursing school. Methods: This was a methodological study. The scale development process included generation of initial items, verification of content validity, selections of secondary items, preliminary study, and extraction of final items. The participants were five graduates 1 to 4 years after graduation (for the preliminary study) and 125 graduates 1 to 4 years after graduation from the nursing school at A University, South Korea. Item analysis, criterion validity, and internal consistency were used to analyze the data. Results: Twenty-six items were selected for the final scale. The scores on the scale were significantly correlated with the Nursing Competency Scale(r=.62, p<.001) and Nursing Performance Scale(r=.63, p<.001). Chronbach's alpha coefficient for the 26 items was .87. Conclusion: The Nursing Practice Capacity scale for evaluating achievement of nursing education objectives has good validity and reliability. The measurement scale can be useful for evaluating the nursing practice capacity of nurses as well as measuring the extent to which graduates have achieved the established education objectives in the education fields.

• Journal of Korean Public Health Nursing
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• v.24 no.1
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• pp.115-125
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• 2010

Purpose: This study was conducted to investigate the knowledge of infectious diseases and the management of children with infectious diseases among daycare facility teachers. Methods: This study was based on survey questionnaires completed by 122 day care facility teachers from 36 daycare center sin one district in Seoul. Results: Seventy three (72.8%) of the participating daycare facility teachers had not received infectious disease prevention education. They recognized that they did not have enough knowledge of infectious diseases. The average knowledge level of infectious diseases was scored as 9.30 on a 0-30 scale, with 0 representing no knowledge and 30 expert knowledge, and compliance level of management of children with infectious diseases was scored as 4.29 on a 0-16 scale, with 0 present no compliance and 16 representing total compliance Confidence of management of children with infectious diseases (r=0.24, p=.031) and the compliance level of management (r=0.35, p=.001) were higher with increased knowledge of infectious diseases. Conclusions: Daycare facility teacher scan lack sufficient knowledge about b infectious diseases. The education about infectious diseases and management of children with infectious diseases is indispensable to prevent trans mission of infectious diseases in daycare facilities.

• Journal of Food Hygiene and Safety
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• v.34 no.4
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• pp.334-339
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• 2019

A procedure based on high-performance liquid chromatography (HPLC) is described to determine ${\beta}-carotene$ in infant formulas. The method for ${\beta}-carotene$ analysis was performed on a C18 reversed-phase column using acetonitrile/methanol/dichloromethane (6:1:3, v/v/v) as a mobile phase. ${\beta}-Carotene$ was determined in HPLC with photo diode array (PDA) detector. The parameters of validation were specificity, linearity, LOD, LOQ, accuracy, precision and repeatability. The specificity was confirmed by the retention time and the linearity ($R^2$), which was over 0.999 in the range of 0.125~2 mg/L. The detection and quantification limits were 0.064 and 0.193 mg/L, respectively. The accuracy and precision of this method using an STD spiked sample were 80~119% and 1.02~2.05% respectively. The method was applied to the analysis of various infant formula and follow-up formulas products containing ${\beta}-carotene$, and all the products contained acceptable levels of ${\beta}-carotene$ for nutrition labeling.

• Genomics & Informatics
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• v.19 no.4
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• pp.37.1-37.7
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• 2021

Genome-wide association studies (GWASs) facilitated the discovery of countless disease-associated variants. However, GWASs have mostly been conducted in European ancestry samples. Recent studies have reported that these European-based association results may reduce disease prediction accuracy when applied in non-Europeans. Therefore, previously reported variants should be validated in non-European populations to establish reliable scientific evidence for precision medicine. In this study, we validated known associations with type 2 diabetes (T2D) and related metabolic traits in 125,850 samples from a Korean population genotyped by the Korea Biobank Array (KBA). At the end of December 2020, there were 8,823 variants associated with glycemic traits, lipids, liver enzymes, and T2D in the GWAS catalog. Considering the availability of imputed datasets in the KBA genome data, publicly available East Asian T2D summary statistics, and the linkage disequilibrium among the variants (r² < 0.2), 2,900 independent variants were selected for further analysis. Among these, 1,837 variants (63.3%) were statistically significant (p ≤ 0.05). Most of the non-replicated variants (n = 1,063) showed insufficient statistical power and decreased minor allele frequencies compared with the replicated variants. Moreover, most of known variants showed <10% genetic heritability. These results could provide valuable scientific evidence for future study designs, the current power of GWASs, and future applications in precision medicine in the Korean population.