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Antioxidative and Cellular Protective Effects of Dolwoe (Gynostemma pentaphyllum Makino) Extracts against Oxidative Stress  

Kim, Kyoung Mi (Department of Fine Chemistry, Seoul National University of Science and Technology, Cosmetic R&D Center, Cosmetic Industry Coupled Collaboration Center)
Kim, A-Rang (Department of Fine Chemistry, Seoul National University of Science and Technology, Cosmetic R&D Center, Cosmetic Industry Coupled Collaboration Center)
Kim, A-Young (Department of Fine Chemistry, Seoul National University of Science and Technology, Cosmetic R&D Center, Cosmetic Industry Coupled Collaboration Center)
Ha, Ji Hun (Department of Fine Chemistry, Seoul National University of Science and Technology, Cosmetic R&D Center, Cosmetic Industry Coupled Collaboration Center)
Xuan, Song Hua (Department of Fine Chemistry, Seoul National University of Science and Technology, Cosmetic R&D Center, Cosmetic Industry Coupled Collaboration Center)
Jeong, Yoon Ju (Department of Fine Chemistry, Seoul National University of Science and Technology, Cosmetic R&D Center, Cosmetic Industry Coupled Collaboration Center)
Park, Young Min (Department of Fine Chemistry, Seoul National University of Science and Technology, Cosmetic R&D Center, Cosmetic Industry Coupled Collaboration Center)
Jeong, Hyo Jin (Department of Fine Chemistry, Seoul National University of Science and Technology, Cosmetic R&D Center, Cosmetic Industry Coupled Collaboration Center)
Hong, In Gi (Department of Fine Chemistry, Seoul National University of Science and Technology, Cosmetic R&D Center, Cosmetic Industry Coupled Collaboration Center)
Park, Soo Nam (Department of Fine Chemistry, Seoul National University of Science and Technology, Cosmetic R&D Center, Cosmetic Industry Coupled Collaboration Center)
Publication Information
Korean Journal of Pharmacognosy / v.48, no.2, 2017 , pp. 125-133 More about this Journal
Abstract
In this study, we investigated the total phenolic and flavonoid contents, component analysis, antioxidative activity and cellular protective effects against oxidative stress on human skin cells in 50% ethanol extract, ethyl acetate fraction and aglycone fraction obtained from Gynostemma pentaphyllum (G. pentaphyllum) Makino. The DPPH (1,1-diphenyl-2-picrylhydrazyl) scavenging activites ($FSC_{50}$) of the 50% ethanol extracts, ethyl acetate fraction and aglycone fraction were 246.8, 147.2, $128.9{\mu}g/mL$, respectively. Reactive oxygen species (ROS) scavenging activities ($OSC_{50}$) of the 50% ethanol extract, ethyl acetate fraction and aglycone fraction on ROS generated in $Fe^{3+}-EDTA/H_2O_2$ system using the luminol-dependent chemiluminescence assay were 37.15, 10.74, $7.19{\mu}g/mL$, respectively. We investigated the cellular protective activity and the results showed that treatment of aglycone fraction ($0.05-0.39{\mu}g/mL$) protect human skin cells in a concentration-dependent manner when the skin cell damages were induced by treating them with $H_2O_2$. These results suggest that extract/fractions of G. pentaphyllum Makino may be applicable as natural antioxidants in cosmetics.
Keywords
Gynostemma pentaphyllum Makino; Antioxidant; Cellular protective effect; Components analysis; Cosmetics;
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1 Yamakoshi, J., Otsuka, F., Sano, A., Tokutake, S., Saito, M., Kikuchi, M. and Kubota, Y. (2003) Lightening effect on ultraviolet-induced pigmentation of guinea pig skin by oral administration of a proanthocyanidin-rich extract from grape seeds. Pig. Cell Res. 16: 629-638.   DOI
2 Magdalena, L. C. and Tak, Y. A. (2010) Reactive oxygen species, cellular redox system, and apoptosis. Free Radic. Biol. Med. 48: 749-762.   DOI
3 Sohala, R. S. and Orrb, W. C. (2012) The redox stress hypothesis of aging. Free Radic. Biol. Med. 52: 539-555.   DOI
4 Kim, J. E., Kim, A. R., Kim, M. J. and Park, S. N. (2011) Antibacterial, antioxidative and antiaging effects of Alliumcepa peel extracts. Appl. Chem. Eng. 22: 178-184.
5 Won, D. H., Gu, H. A., Kim, H. J., Han, S. B., Park, J. and Park, S. N. (2013) Antibacterial and antioxidative activities of Epimedium koreanum Nakai extracts. J. Microbiol. Biotechnol. 41: 284-291.
6 Ha, J. H., Jeong, Y. J., Seong, J. S., Kim, K. M., Kim, A. Y., Fu, M. M., Suh, J. Y., Lee, N. H., Park, J. and Park, S. N. (2015) Antioxidant and antibacterial activities of Glycyrrhiza uralensis Fisher (Jecheon, Korea) extracts obtained by various extract condition. J. Soc. Cosmet. Sci. Korea 41: 361-373.
7 Lee, D. S., Lim, M. S., Kwan, S. S., Kim, S. Y. and Park, S. N. (2012) Antioxidative activity and componential analysis of Chamaecyparis obtusa leaf extract. Appl. Chem. Eng. 23: 93-99.
8 Yaar, M. and Gilchrest, B. A. (2007) Photoageing: mechanism, prevention and therapy. Br. J. Dermatol. 157: 874-887.   DOI
9 Afonso, V., Champy, R., Mitrovic, D., Collin, P. I. and Lomri, A. (2007) Reactive oxygen species and superoxide dismutases : Role in joint diseases. Joint Bone Spine. 74: 324-329.   DOI
10 Park, S. N. (1989) Effects of flavonoids and other phenolic compounds on reactive oxygen mediated biochemical reactions. Seoul National Univ., seoul, Korea.
11 Park, S. N. (2003) Protective effect of isoflavone, genistein from soybean on singlet oxygen induced photohemolysis of human erythrocytes. Korean J. Food Sci. Technol. 35: 510-518.
12 Berman, S. B. and Hastings, T. G. (1997) Inhibition of glutamate transport in synaptosomes by dopamine oxidation and reactive oxygen species. J. Neurochem. 69: 1185-1195.
13 Davies, M. J. (1998) Reactive oxygen species, metalloproteinases, and plaque stability. Am. Heart J. 23: 2382-2383.
14 Bagchi, D., Bagchi, M., Hassoun, E. A. and Stohs, S. J. (1995) In vitro and in vivo generation of reactive oxygen species, DNA damage and lactate dehydrogenase leakage by selected pesticides. Toxicology 104: 129-140.   DOI
15 Park, S. N. (1997) Skin aging and antioxidant. J. Soc. Cosmet. Sci. Korea 23: 75.
16 Park, S. N. (2003) Antioxidative properties of baicalein, component from Scutellaria baicalensis Georgi and its application to cosmetics (I). A. J. Korean Ind. Eng. Chem. 14: 657-665.
17 Hyun, S. H., Lee, J. S., Lee, K. B. and Lee, J. S. (2007) Antioxidative activity of Gynostemma pentaphyllum Makino extracts. Korean J. Food SCI. Technol. 39: 447-451.
18 Xuan, S. H., Kim, G. Y., Yu, J. Y., Kim, J. W., Yang, Y. R., Jeon, Y. H., Jeong, Y. J., Kim, A. R., Park, S. N. (2017) Antioxidant and cellular protective effects against oxidative stress of Calendula officinalis flowers extracts in human skin cells. Appl. Chem. Eng. 27: 620-626.
19 Xuan, S. H., Kim, A. R., Jeong, Y. J., Lee, N. H. (2016) Antioxidant and cellular protective effects of Moringa oleifera leaves extract. J. Soc. Cosmet. Sci. Korea 42: 217-226.
20 Razmovski-Naumovski, V., Huang, T. H. W., Tran, V. H., Li, G. Q., Duke, C. C. and Roufogalis, B. D. (2005) Chemistry and pharmacology of Gynostemma pentaphyllum. Phytochem. Rev. 4: 197-219.   DOI
21 Wang, Z. J. and Luo, D. H. (2007) Antioxidant activities of different fractions of polysaccharide purified from Gynostemma pentaphyllum Makino. Carbohyd. Polymers 68: 54-58.   DOI
22 Srichana, D., Taengtip, R. and Kondo, S. (2011) Antimicrobial activity of Gynostemma pentaphyllum extracts against fungi producing aflatoxin and fumonisin and bacteria causing diarrheal disease. Southeast Asian J. Trop. Med. Public Health 42: 704-710.
23 Huyen, V. T. T., Phan, D. V., Thang, P., Ky, P. T., Hoa , N. K. and Ostenson, C. G. (2012) Antidiabetic effects of add-on Gynostemma pentaphyllum extract therapy with sulfonylureas in type 2 diabetic patients. Evid. Based. Complement. Alternat. Med. 2012: 1-7.
24 Rujjanawate, C., Kanjanapothi, D., and Amornlerdpison, D. (2004) The anti-gastric ulcer effect of Gynostemma pentaphyllum Makino. Phytomedicine 11: 431-435.   DOI
25 Alves, R. C., Costa, A. S. G., Jerez, M., Casal, S., Sineiro, J., Nunez, M. J. and Oliveira, B. (2010) Antiradical activity, phenolics profile, and hydroxymethylfurfural in espresso coffee: influence of technological factors. J. Agric. Food Chem. 58: 12221-12229.   DOI
26 Liu, X., Ye, W., Mo, Z., Yu, B., Zhao, S., Wu, H., Che, C., Jiang, R., Mak, T. C. W. and Wendy Hsiao, W. L. (2004) Five new ocotillone-type saponins from Gynostemma pentaphyllum. J. Nat. Prod. 67: 1147-1151.   DOI
27 Fang, Z. P. and Zeng, X. Y. (1989) Isolation and identification of flavonoids and organic acid from Gynostemma pentaphyllum Makino. Zhongguo Zhongyao Zazhi 14: 676-678.
28 Yang, X., Zhao, Y., Yang, Y. and Ruan, Y. (2008) Isolation and characterization of immunostimulatory polysaccharide from an herb tea, Gynostemma pentaphyllum Makino. J. Agric. Food Chem. 56: 6905-6909.   DOI
29 Zhishen, J., Mengcheng, T. and Jianming, W. (1999) The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem. 64: 555-559.   DOI
30 Kammeyer, A. and Luiten, R. M. (2015) Oxidation events and skin aging. Ageing Res. Rev. 21: 16-29.   DOI
31 Iwashina, T. and Matsumoto, S. (2013) Flavonoid glycosides from the fern, Schizaea (Schizaeaceae) in south pacific region, and their distribution pattern. Bull. Natl. Mus. Nat. Sci. 39: 195-201.
32 Sakakimara, H., Honda, Y., Nakagawa, S., Ashida, H. and Kanazawa, K. (2003) Simultaneous determination of all polyphenols in vegetables, fruits, and teas. J. Agric. Food Chem. 51: 571-581.   DOI
33 Kim, D. S., Kang, Y. M., Jin, W. Y., Sung, Y. Y., Choi, G. and Kim, H. K. (2014) Antioxidant activities and polyphenol content of Morus alba leaf extracts collected from varying regions. Biomed Rep. 2: 675-680.   DOI
34 Sun, J., Liang, F., Bin, Y., Li, P. and Duan, C. (2007) Screening non-colored phenolics in red wines using liquid chromatography/ ultraviolet and mass spectrometry/mass spectrometry libraries. Molecules 12: 679-693.   DOI
35 Hossain, M., Rai, D. K,, Brunton, N. P., Martin-Diana, A. B. and Barry-Ryan, C. (2010) Characterization of phenolic composition in lamiaceae spices by LC-ESI-MS/MS. J. Agric. Food Chem. 58: 10576-10581.   DOI
36 Iwashina, T. and Kokubugata, G. (2012) Flavone and flavonol glycosides from the leaves of Triumfetta procumbens in Ryukyu islands Bull. Natl. Mus. Nat. Sci. 38: 63-67.