• Molecules and Cells
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• 제43권10호
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• pp.856-869
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• 2020

To elucidate the mechanism of action of HOXA11-AS in modulating the cisplatin resistance of nasopharyngeal carcinoma (NPC) cells. HOXA11-AS and miR-454-3p expression in NPC tissue and cisplatin-resistant NPC cells were measured via quantitative reverse transcriptase polymerase chain reaction. NPC parental cells (C666-1 and HNE1) and cisplatin-resistant cells (C666-1/DDP and HNE1/DDP) were transfected and divided into different groups, after which the MTT method was used to determine the inhibitory concentration 50 (IC₅₀) of cells treated with different concentrations of cisplatin. Additionally, a clone formation assay, flow cytometry and Western blotting were used to detect DDP-induced changes. Thereafter, xenograft mouse models were constructed to verify the in vitro results. Obviously elevated HOXA11-AS and reduced miR-454-3p were found in NPC tissue and cisplatin-resistant NPC cells. Compared to the control cells, cells in the si-HOXA11-AS group showed sharp decreases in cell viability and IC₅₀, and these results were reversed in the miR-454-3p inhibitor group. Furthermore, HOXA11-AS targeted miR-454-3p, which further targeted c-Met. In comparison with cells in the control group, HNE1/DDP and C666-1/DDP cells in the si-HOXA11-AS group demonstrated fewer colonies, with an increase in the apoptotic rate, while the expression levels of c-Met, p-Akt/Akt and p-mTOR/mTOR decreased. Moreover, the si-HOXA11-AS-induced enhancement in sensitivity to cisplatin was abolished by miR-454-3p inhibitor transfection. The in vivo experiment showed that DDP in combination with si-HOXA11-AS treatment could inhibit the growth of xenograft tumors. Silencing HOXA11-AS can inhibit the c-Met/AKT/mTOR pathway by specifically upregulating miR-454-3p, thus promoting cell apoptosis and enhancing the sensitivity of cisplatin-resistant NPC cells to cisplatin.

• 한국산학기술학회논문지
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• 제19권4호
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• pp.454-462
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• 2018

본 연구의 목적은 대학생의 SNS중독경향성과 대인관계 만족도를 파악하고, 두 변수간의 상간관계를 파악하여, 대학생의 대인관계 향상 및 SNS 중독 예방을 위한 중재방안의 기초자료를 제공하기 위해 수행되었다. 본 연구의 대상은 D시와 K 지역 대학에 재학 중인 대학생 316명이며, 자료수집 기간은 2017년 6월 30일부터 7월 30일까지였다. 자료수집 방법은 온라인 설문을 이용하여 자가 보고식으로 수집하였고, 자료분석은 SPSS를 이용하여 기술통계, t-test, Pearson correlation coefficients로 분석하였다. 본 연구의 결과 SNS중독경향성과 대인관계만족도는 유의한 음의 상관관계(r=-.57, p<.01)를 보여주어 SNS중독경향성이 높을수록 대인관계 만족도가 낮아짐을 알 수 있었다. 성별(t=.05, p=.963), SNS인맥수(t=.66, p=.513), SNS에서만 유지하는 인맥정도(t=-1.24, p=.216)에 따른 SNS중독경향성의 유의한 차이는 없었다. SNS사용목적 중 정보수집을 위해 SNS이용하는 경우 대인관계 만족도가 더 높았지만(t=3.02, p=.030), SNS사용목적에 따른 SNS중독경향성의 유이한 차이는 없었다(t=0.39, p=.759). 따라서 대학생의 대인관계 만족도를 향상시키기 위한 중재 프로그램 개발 시 본 연구에서 규명된 자료를 기초로 하여 대학생의 SNS중독을 예방하고 대인관계를 향상시키는 프로그램에 활용될 수 있기를 기대한다.

• 한국치위생학회지
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• 제12권2호
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• pp.345-353
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• 2012

Objectives : The author has studied about correlation of gingival exposure upon smiling and oral facial status that reduce facial aesthetic. Methods : The subjects in this study are 91 female vulunteers who were in aged $21.4{\pm}1.89$ in Suwon. Objectives should be normal oral and facial status without the prosthodontic, orthodontic appliance or conqenital missing tooth, and agree to be examined the oral status and impression taking. 1.Measure the length of gingival exposure upon smiling. 2.Measure of the size on central incisor. 3.Measure of Facial. SPSS(SPSS 10.0 for windows, SPSS Inc, Chicago, USA) was utilized for calculating the correlation coefficient between gingival exposure upon smiling and facial status. Regression analysis was calculated in order to predict the R square for gingival exposure upon smiling. Results : 1.Correlation coefficient between the gingival exposure and length of maxillary central incisor was calculated as reversed correlation(r=-.302, p<0.01), and between the gingival exposure and the ratio of the length of central incisor/width of central incisor was revealed as reversed correlation(r=-.250, p<0.05) on smiling. 2.There was correlation between the gingival exposure and the facial height(r=.351, p<0.01), the lower facial height(r=.454, p<0.01) and the upper lip height(r=.274, p<0.01) upon smiling. 3.There was correlation between the gingival exposure and the ratio of the facial height/facial width(r=.358, p<0.05), the ratio of the upper facial height/facial width(r=.214, p<0.05), and the ratio of the lower facial height/facial height(r=.383, p<0.01) upon smiling. 4.The equation of the regression analysis for gingival exposure upon smiling could be estimated as gingival exposure upon smiling=-5.139+.279${\times}$lower facial height-.615${\times}$maxillary central incisal length-.05${\times}$nasolabial angle. Conclusions : Considering these results, it recommended that treatment planning should be designed in consideration of such factors as the length of maxillary central incisor, facial height, upper lip height and lower facial height, in order to promote the easthetic problems of face on smiling.

• 한국방재학회 논문집
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• 제9권3호
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• pp.59-65
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• 2009

본 연구에서는 소방공무원의 직무형태별 외상 후 스트레스와 사회적지지 및 업무부담감을 알아보고 직무별 고위험군을 확인 하고 외상 후 스트레스에 영향을 주는 변수들을 알아보고자 하였다. 자료는 C도에 근무하는 소방대원 중 구급 96명, 구조 및 화재진압 85명, 기관 83명인 총 264 명이 수집되었다. SPSS WIN 14.0 program을 이용하여 총 22문항으로 구성된 외상 후 스트레스 검사 도구 (IES-R), 20문항으로 최은숙(2000)이 수정하여 개발한 업무 부담감 측정도구와 오진환(2006)의 연구에서 수정보완 한 사회적지지도구로 분석하였다. 직무별 일반적 특성에 따른 외상 후 스트레스증상의 결과는 나이, 결혼, 학력, 직급, 재직기간이 세 직무집단 간에서 유의한 차이를 보였다. 직무별 외상 후 스트레스는 구조 및 화재 진압에서 가장 높았으며 세집단 간에 유의한 차이를 보였다. 직무별 세 변수와의 상관관계에서 구조 직무군에서 업무부담감(r=.317, p<0.01)이 많을수록 외상 후 스트레스는 높았고, 구급 직무군에서는 사회적 지지(r=-.331, p<0.01)가 낮을수록, 업무부담감(r=.522 p<0.001)이 많을 수록 외상 후 스트레스가 높았고, 기관 직무군에서는 업무부담감(r=.454, p<0.01)이 많을수록 외상 후 스트레스가 높게 나타났다. 고위험군에 속한 군은 구조 및 화재 진압 군에서는 51명(60%) 구급은 53명(55.2%), 기관은 38명(45.8%)으로 전체 264명 중 142명(54%)이었고 직무별 외상 후 스트레스 고위험군에 영향 주는 변수로는 구조 및 화재 진압군은 요구조자 위험을 경험시 느끼는 충격이었으며(odds ratio=1.216, 95% 신뢰구간: 1.068-1.383) 구급직무군은 업무부담감과(odds ratio=1.100, 95% 신뢰구간: 1.043-1.159) 재직기간(odds ratio= 1.010, 95% 신뢰구간 1.001-1.018)으로 나타났고 기관 직무군에서는 요구조자의 위험을 경험시 느끼는 충격(odds ratio=1.178, 95% 신뢰구간: 1.010-1.373), 나이(odds ratio=1.129, 95% 신뢰구간: 1.020-1.249), 업무부담감(odds ratio= 1.103, 95% 신뢰구간: 1.034-1.177)이었다.

• Journal of Ecology and Environment
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• 제40권1호
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• pp.75-83
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• 2016

Background: In order to evaluate the effect of pH, known as a critical factor for shaping the biogeographical microbial patterns in the studies by others, on the bacterial diversity, we selected two sites in a similar geographical location (site 1; north latitude 35.3, longitude 127.8, site 2; north latitude 35.2, longitude 129.2) and compared their soil bacterial diversity between them. The mountain soil at site 1 (Jiri National Park) represented naturally acidic but almost pollution free (pH 5.2) and that at site 2 was neutral but exposed to the pollutants due to the suburban location of a big city (pH 7.7). Methods: Metagenomic DNAs from soil bacteria were extracted and amplified by PCR with 27F/518R primers and pyrosequenced using Roche 454 GS FLX Titanium. Results: Bacterial phyla retrieved from the soil at site 1 were more diverse than those at site 2, and their bacterial compositions were quite different: Almost half of the phyla at site 1 were Proteobacteria (49 %), and the remaining phyla were attributed to 10 other phyla. By contrast, in the soil at site 2, four main phyla (Actinobacteria, Bacteroidetes, Proteobacteria, and Cyanobacteria) composed 94 %; the remainder was attributed to two other phyla. Furthermore, when bacterial composition was examined on the order level, only two Burkholderiales and Rhizobiales were found at both sites. So depending on pH, the bacterial community in soil at site 1 differed from that at site 2, and although the acidic soil of site 1 represented a non-optimal pH for bacterial growth, the bacterial diversity, evenness, and richness at this site were higher than those found in the neutral pH soil at site 2. Conclusions: These results and the indices regarding diversity, richness, and evenness examined in this study indicate that pH alone might not play a main role for bacterial diversity in soil.