• Title/Summary/Keyword: miR-20a

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The Effects of Mean Grain Size and Organic Matter Contents in Sediments on the Nutrients and Heavy Metals Concentrations (퇴적물 내 입도와 유기물 함량이 영양염류 및 중금속 농도에 미치는 영향)

  • Kim, Seog-Ku;Lee, Mi-Kyung;Ahn, Jae-Hwan;Kang, Sung-Won;Jeon, Sang-Ho
    • Journal of Korean Society of Environmental Engineers
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    • v.27 no.9
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    • pp.923-931
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    • 2005
  • A study was carried out to identify the major causes of sediments pollution in the Paldang Lake in the vicinity of Gyeongan river. Samples from 40 sites were collected and analyzed to characterize the regional distributions of grain size, organic matter contents, and concentrations of T-N, T-P and heavy metals. contaminations. The mean grain size(Mz) ranged from sand type(Mz, $1{\sim}3\;{\phi}$) where Bukhan River and Namhan River converges at a high flow rate to silt type(Mz, $5{\sim}10\;{\phi}$) at the downstream of Gyeongancheon and Paldang lake, reflecting the water circulation in the area. Except sampling point St. 36 near the wetland, the determination coefficient($r^2$) of Mz and organic matter(LOI) was 0.88, showing that more organic matters are concentrated inside finer sediments. The concentrations of T-N and T-P in sediments were higher in the area at which Mz and organic matters are also higher. High concentrations of T-P analyzed in the sediments, ranging from $216{\sim}1,623\;{\mu}g/g$ (Avg. $769\;{\mu}g/g$) could be considered as a critical level. Adsorbed-P and NAI-P, which are easily released to the surrounding environments when physico-chemical characteristics of sediments are changed, was found to be around 20%, which was showed by the result of fractionated-P. Moreover, Principle Component Analysis(PCA), showed that high concentrations of T-N, T-P, organophilic metals (Cd, Cu, Pb, Zn) are distributed in the areas where high organic matter contents and fine grain-sized sediments are found. However, results of $I'_{geo}$ (Geoaccumulation Index) that considers the grain size of sediments showed that heavy metal concentrations in the lake was low enough to be considered as Class 1 indicating the relative degree of pollution was less than zero.

Comparison Study of Water Tension and Content Characteristics in Differently Textured Soils under Automatic Drip Irrigation (자동점적관수에 의한 토성별 수분함량 및 장력 변화특성 비교 연구)

  • Kim, Hak-Jin;Ahn, Sung-Wuk;Han, Kyung-Hwa;Choi, Jin-Yong;Chung, Sun-Ok;Roh, Mi-Young;Hur, Seung-Oh
    • Journal of Bio-Environment Control
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    • v.22 no.4
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    • pp.341-348
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    • 2013
  • Maintenance of adequate soil tension or content during the period of crop growth is necessary to support optimum plant growth and yields. A better understanding of soil tension and content for precision irrigation would allow optimal soil water condition to crops and minimize the adverse effects of water stress on crop growth and development. This research reports on a comparison of soil water tension and content variations in differently textured soils over time under drip irrigation using two different water management methods, i.e. pulse time and required water irrigation methods. The pulse time-based irrigation was performed by turning the solenoid valve on and off for preset times to allow the wetting front to disperse in root zone before additional water was applied. The required water estimation method was a new water control logic designed by Rural Development Administration that applies the amount of water required based on a conversion of the measured water tension into water content. The use of the pulse time irrigation method under drip irrigation at a high tension of -20 kPa and high temperatures over $30^{\circ}C$ was not successful at maintaining moisture tensions within an appropriate range of 5 kPa because the preset irrigation times used for water control could not compensate for the change in evapotranspiration during day and night. The response time and pattern of water contents for all of the tested soils measured with capacitance-based sensor probes were faster and more direct than those of water tensions measured with porous and ceramic cup-based tensiometers when water was applied, indicating water content would be a better control variable for automatic irrigation. The required water estimation-based irrigation method provided relatively stable control of moisture tension, even though somewhat lower tension values were obtained as compared to the target tension of -20 kPa, indicating that growers could expect to be effective in controlling low tensions ranging from -10 to -20 kPa with the required water estimation system.

Properties of the Positive Temperature Coefficient Resistor Behavior on the Na and K Doped BaTiO3 (Na 및 K 치환에 따른 BaTiO3의 Positive Temperature Coefficient Resistor 특성)

  • Lee, Mi-Jai;Lim, Tae-Young;Kim, Sei-Ki;Hwang, Jong-Hee;Kim, Jin-Ho;Seo, Won-Seon
    • Korean Journal of Materials Research
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    • v.20 no.12
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    • pp.654-660
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    • 2010
  • The influences of Na and K content on the crystal phase, the microstructure and the electrical property of $BaTiO_3$-based thermistors was found to show typical PTC effects. The crystal phase of powder calcined at $1000^{\circ}C$ for 4hrs showed a single phase with $BaTiO_3$, and the crystal structure was transformed from tetragonal to cubic phase according to added amounts of Na and K. In XRD results at $43^{\circ}\sim47^{\circ}$, the $(Ba_{0.858}Na_{0.071}K_{0.071})(Ti_{0.9985}Nb_{0.0015})O_{3-\delta}$ showed (002) and (200) peaks but the $(Ba_{0.762}Na_{0.119}K_{0.119})(Ti_{0.9975}Nb_{0.0025})O_{3-\delta}$ showed (002), (020) and (200) peaks. In sintered bodies, those calcined at $600^{\circ}C$ rather than at $1000^{\circ}C$ were dense, and for certain amounts of Na and K showed rapid decreases in grain size. In relative permittivity, the curie temperature due to the transformation of ferroelectric phase rose with added Na and K but decreased in terms of relative permittivity. In the result of the R-T curve, the sintered bodies have curie temperatures of about $140^{\circ}C$ and the resistivity of sintered bodies have scores of $\Omega{\cdot}cm$; the jump order of sintered bodies was shown to be more than $10^4$ in powder calcined at $1000^{\circ}C$.

A study of analytical method for Benzo[a]pyrene in edible oils (식용유지 중 벤조피렌 분석법 비교 연구)

  • Min-Jeong Kim;jun-Young Park;Min-Ju Kim;Eun-Young Jo;Mi-Young Park;Nan-Sook Han;Sook-Nam Hwang
    • Analytical Science and Technology
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    • v.36 no.6
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    • pp.291-299
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    • 2023
  • The benzo[a]pyrene in edible oils is extracted using methods such as Liquid-liquid, soxhlet and ultrasound-assisted extraction. However these extraction methods have significant drawbacks, such as long extraction time and large amount of solvent usage. To overcome these drawbacks, this study attempted to improve the current complex benzo[a]pyrene analysis method by applying the QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) method that can be analyzed in a simple and short time. The QuEChERS method applied in this study includes extraction of benzo[a]pyrene into n-hexane saturated acetonitrile and n-hexane. After extraction and distribution using magnesium sulfate and sodium chloride, benzo[a]pyrene is analyzed by liquid chromatography with fluorescence detector (LC/FLR). As a result of method validation of the new method, the limit of detection (LOD) and quantification (LOQ) were 0.02 ㎍/kg and 0.05 ㎍/kg, respectively. The calibration curves were constructed using five levels (0.1~10 ㎍/kg) and coefficient (R2) was above 0.99. Mean recovery ratio was ranged from 74.5 to 79.3 % with a relative standard deviation (RSD) between 0.52 to 1.58 %. The accuracy and precision were 72.6~79.4 % and 0.14~7.20 %, respectively. All results satisfied the criteria ranges requested in the Food Safety Evaluation Department guidelines (2016) and AOAC official method of analysis (2023). Therefore, the analysis method presented in this study was a relatively simple pretreatment method compared to the existing analysis method, which reduced the analysis time and solvent use to 92 % and 96 %, respectively.

Carbon Reduction Effects of Urban Landscape Trees and Development of Quantitative Models - For Five Native Species - (도시 조경수의 탄소저감 효과와 계량모델 개발 - 5개 향토수종을 대상으로 -)

  • Jo, Hyun-Kil;Kim, Jin-Young;Park, Hye-Mi
    • Journal of the Korean Institute of Landscape Architecture
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    • v.42 no.5
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    • pp.13-21
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    • 2014
  • This study generated regression models to quantify storage and annual uptake of carbon from five native landscape tree species through a direct harvesting method, and established essential information to estimate carbon reduction effects from urban greenspaces. Tree species for the study included the Chionanthus retusus, Prunus armeniaca, Abies holophylla, Cornus officinalis, and Taxus cuspidata, which are usually planted in cities of middle Korea, but for which no information on carbon reduction is available. Ten tree individuals for each species were sampled reflecting various stem diameter sizes at a given interval. The study measured biomass for each part including the roots of sample trees to compute total carbon storage per tree. The annual carbon uptake per tree was quantified by analyzing the radial growth rates of stem samples at breast height or ground level. Regression models were developed using diameter at breast height (dbh) or ground level (dg) as an independent variable to easily estimate storage and annual uptake of carbon per tree for each species. All the regression models showed high fitness with $r^2$ values of 0.92~0.99. Storage and annual uptake of carbon from a tree with dbh of 10 cm were greatest with C. retusus (20.0 kg and 5.9 kg/yr, respectively), followed by P. armeniaca (17.5 kg and 4.5 kg/yr) and A. holophylla (13.2kg and 1.8 kg/yr) in order. A C. officinalis tree and T. cuspidata tree with dg of 10 cm stored 9.3 and 6.3 kg of carbon and annually sequestered 3.2 and 0.6 kg, respectively. The above-mentioned carbon storage equaled the amount of carbon emitted from gasoline consumption of about 23~35 L for C. retusus, P. armeniaca, and A. holophylla, and 11~16 L for C. officinalis and T. cuspidata. A tree with the diameter size of 10 cm annually offset carbon emissions from gasoline use of about 6~10 L for C. retusus, P. armeniaca, and C. officinalis, and 1~3 L for A. holophylla and T. cuspidata. The study breaks new ground to easily quantify biomass and carbon reduction for the tree species by overcoming difficulties in direct cutting and root digging of urban landscape trees.

Assessment of Acquisition Protocols for Post-Radioembolization Yttrium-90 PET/CT: Initial Study (90Y SIR-Spheres를 사용한 암의 간 전이치료에서 PET 영상획득에 관한 초기연구)

  • Bahn, Young Kag;Oh, Shin Hyun;Kim, Jung Yul;Lee, Seung Jae;Park, Joon Young;Shin, Heui Soon;Lim, Han Sang;Kim, Jae Sam
    • The Korean Journal of Nuclear Medicine Technology
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    • v.18 no.1
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    • pp.89-93
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    • 2014
  • Purpose: We investigated the quantitative evaluation of PET/CT images for evaluation after treatment in the treatment of liver metastasis of cancer with $^{90}YSIR$-Sphere. Materials and Methods: Confirmed the correlation between the measured counts was expressed by setting a region of interest from the image and a measure of the dose calibrator to see a correlation diagram of an image in a PET of $^{90}Y$. A portion uptake coefficients between PET images were acquired for 20 minutes LIST mode in 15 patients treated for liver metastasis of cancer using $^{90}Y$ SIR-Spheres high, intermediate portion, a lower portion, three measuring the coefficient of the region of interest is divided into parts, we studied the conditions for proper image acquisition. Results: Coefficient of sites that set the region of interest of the PET image and the measured counts of $^{90}Yappears$ that there is a correlation diagram statistically, ($R^2=0.956$), correlation diagram from the PET image of $^{90}Yis$, PET coefficient the coefficients of all regions of interest were increased in proportion to the image acquisition time, partial ROI coefficients of the PET image is higher about 10 minutes on average, about 14 minutes on average, the central portion is lower part 19 minute average in, confirmed the equilibrium of the standard deviation. Conclusion: Using the isotope $^{90}Y$, it is suitable to obtain a PET image, to obtain the time of proper image, the evaluation of PET/CT images, using the $^{90}Y$ SIR-Spheres and that in the treatment of liver metastases of cancer, it is useful for assessing treatment.

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GnRH Agonist Stimulation Test (GAST) for Prediction of Ovarian Response in Controlled Ovarian Stimulation (COH) (난소기능평가를 위한 Gonadotropin Releasing Hormone Agonist Stimulation Test (GAST)의 효용성에 관한 연구)

  • Kim, Mee-Ran;Song, In-Ok;Yeon, Hye-Jeong;Choi, Bum-Chae;Paik, Eun-Chan;Koong, Mi-Kyoung;Song, Il-Pyo;Lee, Jin-Woo;Kang, Inn-Soo
    • Clinical and Experimental Reproductive Medicine
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    • v.26 no.2
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    • pp.163-170
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    • 1999
  • Objectives: The aims of this study are 1) to determine if GAST is a better indicator in predicting ovarian response to COH compared with patient's age or basal FSH level and 2) to evaluate its role in detecting abnormal ovarian response. Design: Prospective study in 118 patients undergoing IVF-ET using GnRH-a short protocol during May-September 1995. Materials and Methods: After blood sampling for basal FSH and estradiol $(E_2)$ on cycle day two, 0.5ml (0.525mg) GnRH agonist ($Suprefact^{(r)}$, Hoechst) was injected subcutaneously. Serum $E_2$ was measured 24 hours later. Initial $E_2$ difference $({\Delta}E_2)$ was defined as the change in $E_2$ on day 3 over the baseline day 2 value. Sixteen patients with ovarian cyst or single ovary or incorrect blood collection time were excluded from the analysis. The patients were divided into three groups by ${\Delta}E_2$; group A (n=30):${\Delta}E_2$<40 pg/ml, group B (n=52): 40 pg/ml${\leq}{\Delta}E_2$<100 pg/ml, group C (n=20): ${\Delta}E_2{\leq}100$ pg/ml. COH was done by GnRH agonist/HMG/hCG and IVF-ET was followed. Ratio of $E_2$ on day of hCG injection over the number of ampules of gonadotropins used ($E_2hCGday$/Amp) was regarded as ovarian responsiveness. Poor ovarian response and overstimulation were defined as $E_2$ hCGday less than 600 pg/ml and greater than 5000 pg/ml, respectively. Results: Mean age $({\pm}SEM)$ in group A, B and C were $33.7{\pm}0.8^*,\;31.5{\pm}0.6\;and\;30.6{\pm}0.5^*$, respectively ($^*$: p<0.05). Mean basal FSH level of group $A(11.1{\pm}1.1mlU/ml)$ was significantly higher than those of $B(7.4{\pm}0.2mIU/ml)$ and C $(6.8{\pm}0.4mIU/ml)$ (p<0.001). Mean $E_2hCGday$ of group A was significantly lower than those of group B or C, i.e., $1402.1{\pm}187.7pg/ml,\;3153.2{\pm}240.0pg/ml,\;4078.8{\pm}306.4pg/ml$ respectively (p<0.0001). The number of ampules of gonadotropins used in group A was significantly greater than those in group B or C: $38.6{\pm}2.3,\;24.2{\pm}1.1\;and\;18.5{\pm}1.0$ (p<0.0001). The number of oocytes retrieved in group A was significantly smaller than those in group B or C: $6.4{\pm}1.1,\;15.5{\pm}1.1\;and\;18.6{\pm}1.6$, respectively (p<0.0001). By stepwise multiple regression, only ${\Delta}E_2$ showed a significant correlation (r=0.68, p<0.0001) with $E_2HCGday$/Amp, while age or basal FSH level were not significant. Likewise, only ${\Delta}E_2$ correlated significantly with the number of oocytes retrieved (r=0.57, p<0.001). All four patients whose COH was canceled due to poor ovarian response belonged to group A only (Fisher's exact test, p<0.01). Whereas none of 30 patients in group A (0%) had overstimulation, 14 patients among 72 patients (19.4%) in group B and C had overstimulation (Fisher's exact test, p<0.01). Conclusions: These data suggest that initial $E_2$ difference after GAST may be a better prognostic indicator of ovarian response to COH than age or basal FSH level. Since initial $E_2$ difference demonstrates significant association with abnormal ovarian response such as poor ovarian response necessitating cycle cancellation or overstimulation, GAST may be helpful in monitoring and consultation of patients during COH in IVF-ET cycle.

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A Study on Hospital Staff's Perception of Death and Hospice (병원직원들의 죽음 및 호스피스 인식에 관한 연구)

  • Kim, Mi-Jeong
    • Korean Journal of Hospice Care
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    • v.7 no.2
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    • pp.15-25
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    • 2007
  • The purpose of this study was to survey the hospital staff's perception of hospice and death and thereby, suggest the ways to help them have a mature attitude towards and a better understanding of death through an effective education on hospice. For this purpose, this study was designed to provide some data useful for the hospital staff not experienced in facing the dying patients to handle the desperate situation skillfully and engage themselves more effectively in their hospice services. For this study, the researcher conducted a questionnaire survey for the staff of 'C' hospital in Seoul about their hospice philosophy, attitudes towards hospice and perception of death for the period from February, 2006 to March, 2006. A total of 751 subjects responded effectively to the survey. The data collected were analyzed using the SF55 11.0 for ANOVA and T-test in order to test the relationships among subjects' perception of death, their demographic variables, their health condition, their hospice philosophy formed from their experiences of patients' death and hospice services and their attitudes towards hospice. Besides, the correlations among their hospice philosophy, perception of death and attitudes towards hospice were tested. The results of this study can be summarized as follows; First, as a result of testing the relationships among subjects' experiences of health consulting. their experiences of patients' death and hospice and their hospice philosophy, it was confirmed that their experiences of health and death consulting and their experiences of having been educated were relevant. Second, it was found that such variables as health condition, death, experience of hospice and attitude towards hospice were not significantly correlated with each other. Third, as a consequence of testing the relationships among health condition, death, experience of hospice and perception of death, it was disclosed that only the physical health condition was significant. Fourth, it was confirmed that subjects' hospice philosophy, perception of death and attitudes towards hospice were at the usual level on average. Fifth, hospice philosophy, perception of death and attitudes towards hospice were found correlated significantly with each other. Sixth, as a result of the stepwise variable adjustment for such variables as hospice philosophy, perception of death, attitudes towards hospice, it was found that the adjusted r-square value was 0.347 when departments, experience of having been requested by dying patient for consulting, experience of having been educated on hospice, religion and marital status were set as independent variables. And the estimated value of each variable was found significant. Seventh, as a consequence of conducting the multiple regression analysis by setting 'religion' as significant independent variable, it was found that the estimated value of physical health condition was not significant statistically. This, as a result of the simple regression analysis for 'religion' only, its explanatory power was found .197, while its adjusted r-square value was 0.20. Eight, it was found that subjects' attitude towards hospice was significantly correlated with such variables of experience of patient's or relative's death, experience of having been requested for consulting about death, gender, marital status and departments. As a result of the multiple regression analysis and the subsequent stepwise adjustment for this variable, it was found that only 'experience of having been requested for consulting' had some explanatory power: its adjusted r-square value was 0.089. As discussed above, this study tested the correlations among various variables including hospital staff's attitude towards hospice and perception of death and thereby, provided for the data useful for their education on hospice. This study may be significant in that it proved that it would be essential to educate hospital staff on hospice for more effective care of hospice patients and their family members at hospital.

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Rainfall Interception by and Quantitative Models for Urban Landscape Trees - For Seven Native Species - (도시조경수의 우수차집 효과와 계량모델 - 7개 향토수종을 대상으로 -)

  • Park, Hye-Mi;Jo, Hyun-Kil;Kim, Jin-Young
    • Journal of the Korean Institute of Landscape Architecture
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    • v.49 no.4
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    • pp.30-40
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    • 2021
  • This study developed quantitative models to estimate the rainfall interception by seven native landscape tree species based on throughfall measurements. The tree species considered in this study were Abies holophylla, Acer palmatum, Ginkgo biloba, Pinus densiflora, Pinus koraiensis, Prunus yedoensis, and Zelkova serrata, which are frequently planted in the Korea. Among these species, 35.8% of the annual precipitation was intercepted by P. koraiensis, 34.1% by A. holophylla, 31.0% by Z. serrata, 27.6% by P. densiflora, 26.9% by G. biloba, 18.6% by A. palmatum, and 18.4% by P. yedoensis. All the quantitative models showed high fitness with r2 values of 0.90-0.99. The annual rainfall interception from a tree with DBH of 20 cm were greatest with Z. serrata (5.1 m3/yr), followed by P. koraiensis (4.1 m3/yr), A. holophylla (3.1 m3/yr), G. biloba (2.8 m3/yr), P. densiflora (2.1 m3/yr), P. yedoensis (1.9 m3/yr), and A. palmatum (1.8 m3/yr) in order. Thus, evergreen tree species or those with a relatively high crown density were more effective in intercepting rainfall. In particular, the annual rainfall interception by Z. serrata was the greatest because its crown area, volume, and density were higher than those of the other species. This study pioneers in quantifying annual rainfall interception for landscape tree species in Korea. The study results can be useful for evaluating rainfall interception by landscape trees in urban greenspace design for governments and corporations.

Analysis of Nitrosation Inhibition and Antioxidant Effect by Phyto-Extract Mixture (식물추출 혼합제재인 phyto-extract mixture의 니트로세이션 억제능과 항산화능 분석)

  • Kim, Ji-Hoon;Shin, Mi-Jung;Cho, Hee-Jae;Lee, Sang-Won;Jeong, Jong-Moon
    • Korean Journal of Food Science and Technology
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    • v.33 no.6
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    • pp.656-663
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    • 2001
  • The most representative nitrosamine derived from nicotine, nitrosamine-4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone(NNK), has been reported to cause lung cancer in A/J mice. It has been also demonstrated that NNK-induced lung tumorigenesis involves $O^6-methylguanine(O^6MeG)$ formation, leading to $GC\;{\rightarrow}\;AT$ transitional mispairing during DNA replication. Our in vitro experiment, modified from the method of DBA assay, examined the ability of phyto-extract mixture to inhibit the metabolism of nicotine to nitrosamines. The production of nitromorpholine from morpholine was inhibited about 75% at the concentration of 20 mg/mL of phyto-extract mixture, which was lower than vitamine C and green tea powder. NNK, which is a pro-carcinogen in laboratory animals, is hydroxylated primarily in liver and lung by CYP 1A2, 2A6 and 3A4. A critical phase. of NNK activation is its change to an unstable metabolite methyl-diazohydroxide via CYP-mediated ${\alpha}-hydroxylation$; and then it provides a methyl group to the DNA to form DNA adducts which can easily induce mutations. $Aroclor^R$ 1254 was used to induce CYPs in the liver of a Sprague-Dawley rat. The ability of various test samples to inhibit CYPs that participate in NNK activation was evaluated, following the removal of the liver from the rat. Microsomal CYPlA2 catalyzing the conversion of NNK into strong carcinogenic chemicals was inhibited more efficiently by phyto-extract mixture than green tea powder. These results indicate that phyto-extract mixture can be used to reduce $O^6MeG$ DNA adducts for chemoprevention.

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