• Animal Bioscience
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• 제36권9호
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• pp.1336-1349
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• 2023

Objective: The study was conducted to screen differentially expressed miRNAs in sows at early pregnancy by high-throughput sequencing and explore its mechanism of action on embryo implantation. Methods: The blood serum of pregnant and non-pregnant Landrace×Yorkshire sows were collected 14 days after artificial insemination, and exosomal miRNAs were purified for high throughput miRNA sequencing. The expression patterns of 10 differentially expressed (DE) miRNAs were validated by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). The qRT-PCR quantified the abundance of serum exosomal miR-192 in pregnant and control sows, and the diagnostic power was assessed by receiver operating characteristic (ROC) analysis. The target genes of DE miRNAs were predicted with bioinformatics software, and the functional and pathway enrichment analysis was performed on gene ontology and the Kyoto encyclopedia of genes and genomes terms. Furthermore, a luciferase reporter system was used to identify the target relation between miR-192 and integrin alpha 4 (ITGA4), a gene influencing embryo implantation in pigs. Finally, the expression levels of miRNAs and the target gene ITGA4 were analyzed by qRT-PCR, and western blot, with the proliferation of BeWo cells detected by cell counting kit-8 (CCK-8). Results: A total of 221 known miRNAs were detected in the libraries of the pregnant and non-pregnant sows, of which 55 were up-regulated and 67 were down-regulated in the pregnant individuals compared with the non-pregnant controls. From these, the expression patterns of 10 DE miRNAs were validated. The qRT-PCR analysis further confirmed a significantly higher expression of miR-192 in the serum exosomes extracted from pregnant sows, when compared to controls. The ROC analysis revealed that miR-192 provided excellent diagnostic accuracy for pregnancy (area under the ROC curve [AUC]=0.843; p>0.001). The dual-luciferase reporter assay indicated that miR-192 directly targeted ITGA4. The protein expression of ITGA4 was reduced in cells that overexpressed miR-192. Overexpression of miR-192 resulted in the decreased proliferation of BeWo cells and regulated the expression of cell cycle-related genes. Conclusion: Serum exosomal miR-192 could serve as a potential biomarker for early pregnancy in pigs. miR-192 targeted ITGA4 gene directly, and miR-192 can regulate cellular proliferation.

• Nutrition Research and Practice
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• 제14권4호
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• pp.412-422
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• 2020

BACKGROUND/OBJECTIVES: This study investigates correlations between circulating microRNAs (miRNAs) and obesity-related parameters among young women (aged 20-30 years old) in Korea. SUBJECTS/METHODS: We analyzed TaqMan low density arrays (TLDAs) of circulating miRNAs in 9 lean (body mass index [BMI] < 25 kg/㎡) and 15 obese (BMI > 25 kg/㎡) women. We also performed gene ontology (GO) analyses of the biological functions of predicted miRNA target genes, and clustered the results using the database for annotation, visualization and integrated discovery. RESULTS: The TLDA cards contain 754 human miRNAs; of these, the levels of 8 circulating miRNAs significantly declined (> 2-fold) in obese subjects compared with those in lean subjects, including miR-1227, miR-144-5p, miR-192, miR-320, miR-320b, miR-484, miR-324-3p, and miR-378. Among them, miR-484 and miR-378 displayed the most significant inverse correlations with BMI (miR-484, r = -0.5484, P = 0.0056; miR-378, r = -0.5538, P = 0.0050) and visceral fat content (miR-484, r = -0.6141, P = 0.0014; miR-378, r = -0.6090, P = 0.0017). GO analysis indicated that genes targeted by miR-484 and miR-378 had major roles in carbohydrate and lipid metabolism. CONCLUSION: Our result showed the differentially expressed circulating miRNAs in obese subjects compared to lean subjects. Although the mechanistic study to reveal the causal role of miRNAs remains, these miRNAs may be novel biomarkers for obesity.

• Biomolecules & Therapeutics
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• 제25권4호
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• pp.367-373
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• 2017

This study was performed to evaluate whether microRNAs (miRNAs) in circulating exosomes may serve as biomarkers of drug-induced liver, kidney, or muscle-injury. Quantitative PCR analyses were performed to measure the amounts of liver-specific miRNAs (miR-122, miR-192, and miR-155), kidney-specific miR-146a, or muscle-specific miR-206 in plasma and exosomes from mice treated with liver, kidney or muscle toxicants. The levels of liver-specific miRNAs in circulating plasma and exosomes were elevated in acetaminophen-induced liver injury and returned to basal levels by treatment with antioxidant N-acetyl-cysteine. Circulating miR-146a and miR-206 were increased in cisplatin-induced nephrotoxicity and bupivacaine-induced myotoxicity, respectively. Taken together, these results indicate that circulating plasma and exosomal miRNAs can be used as potential biomarkers specific for drug-induced liver, kidney or muscle injury.

• Journal of Veterinary Science
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• 제21권6호
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• pp.81.1-81.10
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• 2020

Background: Although previous in vivo studies explored urinary microRNA (miRNA), there is no agreement on nephrotoxicity-specific miRNA biomarkers. Objectives: In this study, we assessed whether urinary miRNAs could be employed as biomarkers for nephrotoxicity. Methods: For this, literature-based candidate miRNAs were identified by reviewing the previous studies. Female Sprague-Dawley rats received subcutaneous injections of a single dose or repeated doses (3 consecutive days) of gentamicin (GEN; 137 or 412 mg/kg). The expression of miRNAs was analyzed by real-time reverse transcription-polymerase chain reaction in 16 h pooled urine from GEN-treated rats. Results: GEN-induced acute kidney injury was confirmed by the presence of tubular necrosis. We identified let-7g-5p, miR-21-3p, 26b-3p, 192-5p, and 378a-3p significantly upregulated in the urine of GEN-treated rats with the appearance of the necrosis in proximal tubules. Specifically, miR-26-3p, 192-5p, and 378a-3p with highly expressed levels in urine of rats with GEN-induced acute tubular injury were considered to have sensitivities comparable to clinical biomarkers, such as blood urea nitrogen, serum creatinine, and urinary kidney injury molecule protein. Conclusions: These results indicated the potential involvement of urinary miRNAs in chemical-induced nephrotoxicity, suggesting that certain miRNAs could serve as biomarkers for acute nephrotoxicity.

• 한국가스학회:학술대회논문집
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• 한국가스학회 1998년도 추계학술발표회 논문집
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• pp.188-192
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• 1998

• Nuclear Engineering and Technology
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• 제39권3호
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• pp.187-192
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• 2007

The basic research on fast reactor technology was started in the mid-1960's in China. The emphasis was put on fast reactor neutronics, thermohydraulics, sodium technology, materials, fuels, safety, sodium devices and instrumentation. In 1987, the research turned to applied basic research with the conceptual design of a 60 MW experimental fast reactor as a target. The Project of the China Experimental Fast Reactor(CEFR) with a thermal power 65 MW was launched in 1993. The R&D of fast reactor technology then carried out to serve a design demonstration connected with the different phases of the conceptual, preliminary and detailed design of the CEFR. Recently, three directions of fast rector technology R&D activities have been considered, and some research programs have been developed. They are: (1) R&D related to the CEFR, i.e. experiments to be conducted on the CEFR for its safe operation, (2) R&D related to the projects of a prototype and the demonstration of fast reactors, and(3) advanced SFR technology within the framework of the international cooperation of INPRO and GIF.

• 한국산학기술학회:학술대회논문집
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• 한국산학기술학회 2009년도 춘계학술발표논문집
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• pp.616-619
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• 2009

국내 육성 품종 JW07-190, JW07-192과 일본산 도입 품종 Meiryoku (MR), Ryoufuu (RF), Yamanoibuki (YI) 대해서 80% 메탄올 추출물을 공시 재료로 ESR을 이용하여 항산화 효능을 평가하였다. 국내 육성 품종인 JW07-192에서 DPPH radical, alkyl radical, hydroxyl radical에 대해 비교적 높은 radical 소거능을 보였고, 모든 녹차 품종에서 강력한 DPPH radical 소거능을 보였다. 전체적으로, 국내 육성 품종인 JW07-192가 일본 도입 품종과 비교하였을 때 더 좋은 항산화 효능을 나타내었다. 총 폴리페놀 함량은 국내 육성 계통인 JW07-192가 $431.57{\pm}28.45\;mg/g$로 가장 높은 함량을 나타냈고, 플라보노이드 함량은 도입 품종인 YI가 $313.44{\pm}6.31$로 가장 높은 함량을 보였다.

• 디지털융복합연구
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• 제20권4호
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• pp.127-132
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• 2022

본 연구는 간호대 학생의 리더십, 의사 소통 능력, 동료 돌봄 행위 정도 및 관계를 파악하기 위한 서술적 조사연구이다. 연구 대상자는 G광역시 소재 간호대 학생 192명으로 자료수집은 2021년 11월 10일부터 202년 11월 30일까지 이루어졌다, 수집된 자료는 SPSS 28.0 Program을 이용하여 평균과 표준편차, 백분율, ANOVA, t-test, Pearson's 상관관계를 분석하였다. 연구결과 간호대 학생의 리더쉽과 의사 소통 능력(r=.634, p<.001), 의사 소통 능력과 동료 돌봄 행위(r=.390, p<.001), 리더쉽과 동료 돌봄 행위(r=.285, p<.001) 간 유의한 상관관계를 보였다. 따라서 향후 간호대 학생들의 리더쉽과 의사 소통 능력, 동료 돌봄 행위를 향상시키기 위한 교육적 환경제공과 다양한 교육 프로그램 개발과 효과검증을 위한 연구가 필요하다.

• 문화기술의 융합
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• 제9권6호
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• pp.435-442
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• 2023

본 연구는 간호대학생의 우울, 자기효능감, 생활스트레스와 스마트폰 중독의 정도를 확인하고 이들의 상관관계 및 영향요인을 파악하기 위한 서술적 조사연구이다. 연구대상자의 우울 15.85점, 자기효능감 3.19점, 생활스트레스 2.47점, 스마트폰 중독 39.23점으로 나타났다. 일반적 특성에 따른 스마트폰 중독을 분석한 결과는 연령에서는 통계적으로 유의한 차이가 없는 것으로 나타났으며, 성별(t=-2.540, p=.014), 학년(F=3.080, p=.029), 스마트폰 중요도(F=16.192, p<.001), 스마트폰 사용시간(F=6.938, p<.001)에서는 유의한 차이가 있었다. 대상자의 스마트폰 중독은 우울(r=.351, p<0.01), 생활스트레스(r=.321, p<.001)와는 정적상관관계가 나타났으며, 자기효능감,(r=-.383, p<.001)은 부적 상관관계가 나타났다. 우울과 자기효능감(r=-.529, p<.001)은 부적 상관관계가 나타났으며, 생활스트레스(r=.568, p<.001)은 정적 상관관계가 나타났다. 자기효능감과 생활스트레스는(r=-.434, p<.001)부적 상관관계가 나타났다. 대상자의 스마트폰 중독의 영향요인으로는 자기효능감이 나타났다. 대학생의 자기효능감을 향상시키기 위한 프로그램 및 교육의 기초자료를 제공하고자 한다.

• 한국직업건강간호학회지
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• 제19권1호
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• pp.50-59
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• 2010

This study was done to examine ER nurses' burn-out, as an understanding in the relationship of nursing performance and professional identity. The subjects of the study were nurses working in the emergency room of the general hospital with over 300 beds, located in D metropolitan city and C city. The self-report questionnaires were administered and 120 were collected. For the data analysis, SPSS WIN 12.0 was used. ER nurses' burn-out was 2.99, the mean of nursing performance was 3.69, and professional identity was 3.51. The extent of ER nurses' burn-out showed significantly negative correlation with the extent of nursing performance(r=-.257), along with professional identity(r=-.192). While ER nurses' nursing performance showed significantly positive correlation with professional identity(r=.696). In conclusion, the higher professional identity, the fewer ER nurses experienced burn-out, and the higher extent of nursing performance was obtained. It is necessary to create activities and programs to reduce and prevent burn-out.