• Animal Bioscience
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• v.36 no.9
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• pp.1336-1349
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• 2023

Objective: The study was conducted to screen differentially expressed miRNAs in sows at early pregnancy by high-throughput sequencing and explore its mechanism of action on embryo implantation. Methods: The blood serum of pregnant and non-pregnant Landrace×Yorkshire sows were collected 14 days after artificial insemination, and exosomal miRNAs were purified for high throughput miRNA sequencing. The expression patterns of 10 differentially expressed (DE) miRNAs were validated by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). The qRT-PCR quantified the abundance of serum exosomal miR-192 in pregnant and control sows, and the diagnostic power was assessed by receiver operating characteristic (ROC) analysis. The target genes of DE miRNAs were predicted with bioinformatics software, and the functional and pathway enrichment analysis was performed on gene ontology and the Kyoto encyclopedia of genes and genomes terms. Furthermore, a luciferase reporter system was used to identify the target relation between miR-192 and integrin alpha 4 (ITGA4), a gene influencing embryo implantation in pigs. Finally, the expression levels of miRNAs and the target gene ITGA4 were analyzed by qRT-PCR, and western blot, with the proliferation of BeWo cells detected by cell counting kit-8 (CCK-8). Results: A total of 221 known miRNAs were detected in the libraries of the pregnant and non-pregnant sows, of which 55 were up-regulated and 67 were down-regulated in the pregnant individuals compared with the non-pregnant controls. From these, the expression patterns of 10 DE miRNAs were validated. The qRT-PCR analysis further confirmed a significantly higher expression of miR-192 in the serum exosomes extracted from pregnant sows, when compared to controls. The ROC analysis revealed that miR-192 provided excellent diagnostic accuracy for pregnancy (area under the ROC curve [AUC]=0.843; p>0.001). The dual-luciferase reporter assay indicated that miR-192 directly targeted ITGA4. The protein expression of ITGA4 was reduced in cells that overexpressed miR-192. Overexpression of miR-192 resulted in the decreased proliferation of BeWo cells and regulated the expression of cell cycle-related genes. Conclusion: Serum exosomal miR-192 could serve as a potential biomarker for early pregnancy in pigs. miR-192 targeted ITGA4 gene directly, and miR-192 can regulate cellular proliferation.

• Nutrition Research and Practice
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• v.14 no.4
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• pp.412-422
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• 2020

BACKGROUND/OBJECTIVES: This study investigates correlations between circulating microRNAs (miRNAs) and obesity-related parameters among young women (aged 20-30 years old) in Korea. SUBJECTS/METHODS: We analyzed TaqMan low density arrays (TLDAs) of circulating miRNAs in 9 lean (body mass index [BMI] < 25 kg/㎡) and 15 obese (BMI > 25 kg/㎡) women. We also performed gene ontology (GO) analyses of the biological functions of predicted miRNA target genes, and clustered the results using the database for annotation, visualization and integrated discovery. RESULTS: The TLDA cards contain 754 human miRNAs; of these, the levels of 8 circulating miRNAs significantly declined (> 2-fold) in obese subjects compared with those in lean subjects, including miR-1227, miR-144-5p, miR-192, miR-320, miR-320b, miR-484, miR-324-3p, and miR-378. Among them, miR-484 and miR-378 displayed the most significant inverse correlations with BMI (miR-484, r = -0.5484, P = 0.0056; miR-378, r = -0.5538, P = 0.0050) and visceral fat content (miR-484, r = -0.6141, P = 0.0014; miR-378, r = -0.6090, P = 0.0017). GO analysis indicated that genes targeted by miR-484 and miR-378 had major roles in carbohydrate and lipid metabolism. CONCLUSION: Our result showed the differentially expressed circulating miRNAs in obese subjects compared to lean subjects. Although the mechanistic study to reveal the causal role of miRNAs remains, these miRNAs may be novel biomarkers for obesity.

• Biomolecules & Therapeutics
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• v.25 no.4
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• pp.367-373
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• 2017

This study was performed to evaluate whether microRNAs (miRNAs) in circulating exosomes may serve as biomarkers of drug-induced liver, kidney, or muscle-injury. Quantitative PCR analyses were performed to measure the amounts of liver-specific miRNAs (miR-122, miR-192, and miR-155), kidney-specific miR-146a, or muscle-specific miR-206 in plasma and exosomes from mice treated with liver, kidney or muscle toxicants. The levels of liver-specific miRNAs in circulating plasma and exosomes were elevated in acetaminophen-induced liver injury and returned to basal levels by treatment with antioxidant N-acetyl-cysteine. Circulating miR-146a and miR-206 were increased in cisplatin-induced nephrotoxicity and bupivacaine-induced myotoxicity, respectively. Taken together, these results indicate that circulating plasma and exosomal miRNAs can be used as potential biomarkers specific for drug-induced liver, kidney or muscle injury.

• Journal of Veterinary Science
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• v.21 no.6
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• pp.81.1-81.10
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• 2020

Background: Although previous in vivo studies explored urinary microRNA (miRNA), there is no agreement on nephrotoxicity-specific miRNA biomarkers. Objectives: In this study, we assessed whether urinary miRNAs could be employed as biomarkers for nephrotoxicity. Methods: For this, literature-based candidate miRNAs were identified by reviewing the previous studies. Female Sprague-Dawley rats received subcutaneous injections of a single dose or repeated doses (3 consecutive days) of gentamicin (GEN; 137 or 412 mg/kg). The expression of miRNAs was analyzed by real-time reverse transcription-polymerase chain reaction in 16 h pooled urine from GEN-treated rats. Results: GEN-induced acute kidney injury was confirmed by the presence of tubular necrosis. We identified let-7g-5p, miR-21-3p, 26b-3p, 192-5p, and 378a-3p significantly upregulated in the urine of GEN-treated rats with the appearance of the necrosis in proximal tubules. Specifically, miR-26-3p, 192-5p, and 378a-3p with highly expressed levels in urine of rats with GEN-induced acute tubular injury were considered to have sensitivities comparable to clinical biomarkers, such as blood urea nitrogen, serum creatinine, and urinary kidney injury molecule protein. Conclusions: These results indicated the potential involvement of urinary miRNAs in chemical-induced nephrotoxicity, suggesting that certain miRNAs could serve as biomarkers for acute nephrotoxicity.

• 한국가스학회:학술대회논문집
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• 1998.09a
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• pp.188-192
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• 1998

• Nuclear Engineering and Technology
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• v.39 no.3
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• pp.187-192
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• 2007

The basic research on fast reactor technology was started in the mid-1960's in China. The emphasis was put on fast reactor neutronics, thermohydraulics, sodium technology, materials, fuels, safety, sodium devices and instrumentation. In 1987, the research turned to applied basic research with the conceptual design of a 60 MW experimental fast reactor as a target. The Project of the China Experimental Fast Reactor(CEFR) with a thermal power 65 MW was launched in 1993. The R&D of fast reactor technology then carried out to serve a design demonstration connected with the different phases of the conceptual, preliminary and detailed design of the CEFR. Recently, three directions of fast rector technology R&D activities have been considered, and some research programs have been developed. They are: (1) R&D related to the CEFR, i.e. experiments to be conducted on the CEFR for its safe operation, (2) R&D related to the projects of a prototype and the demonstration of fast reactors, and(3) advanced SFR technology within the framework of the international cooperation of INPRO and GIF.

• Proceedings of the KAIS Fall Conference
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• 2009.05a
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• pp.616-619
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• 2009

국내 육성 품종 JW07-190, JW07-192과 일본산 도입 품종 Meiryoku (MR), Ryoufuu (RF), Yamanoibuki (YI) 대해서 80% 메탄올 추출물을 공시 재료로 ESR을 이용하여 항산화 효능을 평가하였다. 국내 육성 품종인 JW07-192에서 DPPH radical, alkyl radical, hydroxyl radical에 대해 비교적 높은 radical 소거능을 보였고, 모든 녹차 품종에서 강력한 DPPH radical 소거능을 보였다. 전체적으로, 국내 육성 품종인 JW07-192가 일본 도입 품종과 비교하였을 때 더 좋은 항산화 효능을 나타내었다. 총 폴리페놀 함량은 국내 육성 계통인 JW07-192가 $431.57{\pm}28.45\;mg/g$로 가장 높은 함량을 나타냈고, 플라보노이드 함량은 도입 품종인 YI가 $313.44{\pm}6.31$로 가장 높은 함량을 보였다.

• Journal of Digital Convergence
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• v.20 no.4
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• pp.127-132
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• 2022

This study is a descriptive research to understand the leadership, communication ability, peer care behavior and relationship of nursing students. Data were collected from 192 nursing students in November 2021. The data were analyzed by t-test, ANOVA, Pearson's correlation ciefficients, using SPSS 28.0. As a result of the study, revealed positive correlations between leaderships and communication competence(r=.634, p<.001), communication competence and peer group caring interaction(r=.390, p<.001), leaderships and peer group caring interaction(r=.285, p<.001). Therefore, it is necessary to develop educational environment and programs to improve leadership, communication ability, and peer care behavior of nursing college students in the future.

• The Journal of the Convergence on Culture Technology
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• v.9 no.6
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• pp.435-442
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• 2023

This study is a descriptive survey study to confirm the degree of depression, self-efficacy, life stress and smartphone addiction of nursing college students, and to identify their correlations and influencing factors. The study subjects had 15.85 points of depression, 3.19 points of self-efficacy, 2.47 points of life stress, and 39.23 points of smartphone addiction. As a result of analyzing smartphone addiction according to the general characteristics of the subjects, there was no statistically significant difference in grade and major, and there were significant differences in gender(t=-2.540, p=.014), grade(F=3.080, p=.029), smartphone importance(F=16.192, P<0.001), smartphone usage time (F=6.938, P<0.001). The subject's smartphone addiction showed a positive correlation with depression (r=.351, p<0.01), life stress (r=.321, p<.001), and self-efficacy (r=-.383, p<.001) showed a negative correlation. A negative correlation was found between depression and self-efficacy (r=-.529, p<.001), and a positive correlation was found between life stress (r=.568, p<.001). A negative correlation was found between self-efficacy and college life stress (r=-.434, p<.001). Self-efficacy appeared to be an influential factor in the subject's smartphone addiction. The goal is to provide basic data for programs and education to improve college students' self-efficacy.

• Korean Journal of Occupational Health Nursing
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• v.19 no.1
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• pp.50-59
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• 2010

This study was done to examine ER nurses' burn-out, as an understanding in the relationship of nursing performance and professional identity. The subjects of the study were nurses working in the emergency room of the general hospital with over 300 beds, located in D metropolitan city and C city. The self-report questionnaires were administered and 120 were collected. For the data analysis, SPSS WIN 12.0 was used. ER nurses' burn-out was 2.99, the mean of nursing performance was 3.69, and professional identity was 3.51. The extent of ER nurses' burn-out showed significantly negative correlation with the extent of nursing performance(r=-.257), along with professional identity(r=-.192). While ER nurses' nursing performance showed significantly positive correlation with professional identity(r=.696). In conclusion, the higher professional identity, the fewer ER nurses experienced burn-out, and the higher extent of nursing performance was obtained. It is necessary to create activities and programs to reduce and prevent burn-out.