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ISOLATION AND IDENTIFICATION OF LACTOBACILLUS SALIVARIUS INHIBITING THE FORMATION OF ARTIFICIAL PLAQUE AND THE PRODUCTION OF VOLATILE SULFUR COMPOUNDS (치태 형성과 휘발성 유황화합물 생성을 억제하는 Lactobacillus salivarius의 분리 및 동정)

  • Kim, Mi-Hyung;Choi, Nam-Ki;Kim, Seon-Mi;Oh, Jung-Suk;Yang, Kyu-Ho
    • Journal of the korean academy of Pediatric Dentistry
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    • v.32 no.2
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    • pp.344-356
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    • 2005
  • There are normal inhabitants doing medically useful functions in the body. There are many kinds of bacteria performing specific functions in the oral cavity. Two strains of lactic acid bacteria were isolated from inhabitants of caries-free children's oral cavity, which inhibited the formation of artificial plaque by Streptococcus mutans and the production of volatile sulfur compounds by anaerobic bacteria. The isolates were identified by the test using API 50 CHL medium kit and 16S rDNA partial sequencing. 1. Two isolates were Gram-positive bacilli and produced hydrogen peroxide. 2. When Streptococcus mutans was cultured in the media, the mean weight of formed artificial plaque on the orthodontic wires was $124.4{\pm}30.4\;mg$, whereas being reduced to $5.2{\pm}2.0mg$ and $10.6{\pm}6.6mg$ in the media cultured with Streptococcus mutans and each isolate, respectively (p<0.05) 3. The number of viable cells of Streptococcus mutans was $3.4{\times}10^9$ per ml in the cultured solution, whereas those of Streptococcus mutans in the combined culture with each of isolates were $4.6{\times}10^8\;and\;2.4{\times}10^8$ per ml. 4. The optical density was 1.286 in the supernatant of Fusobacterium nucleatum after vortexing for 30minutes, whereas in the supernatant of combined Fusobacterium nucleatum and each isolate, they were reduced to 0.628 and 0.497, which the percentages of coaggregation between them were 29.4% and 57.8%, respectively 5. The optical density of Fusobacterium nucleatum precipitate was 1.794 in the culture media containing cysteine and $FeSO_4$, being reduced to 1.144 and 0.915 in the coaggregated precipitates of Fusobacterium nucleatum and each isolate. The optical density of Porphyromonas gingivalis precipitate was 1.932 in the culture media, being reduced to 1.170 and 1.266 in the coaggregated precipitates of Porphyromonas gingivalis and each isolate. 6. When two isolates were tested with API 50 CHL medium kit, those were identified Lactobaciallius salivarius and Lactobaciallius delbrueckii subsp. lactis. 7. The similarity values of 16S rDNA sequence between each of isolates and Lactobaciallius salivarius subsp. salicinius were 99.60% and 99.73%, respectively, meaning that isolates were Lactobaciallius salivarius subsp. salicinius. These results indicated that two strains isolated from caries-free children's saliva, which inhibited the formation of artificial plaque and the production of volatile sulfur compounds, were identified as Lactobaciallius salivarius subsp. salicinius.

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Effect of Myers-Briggs Type Indicator(MBTI) Personality Type, Emotional Intelligence, Sress Coping Strategies on Nursing student's Adjustment to College Life (간호대학생의 MBTI성격유형, 감성지능, 스트레스 대처방식이 대학생활적응에 미치는 영향)

  • Han, Young-Mi;Kim, Sun-Hee
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.18 no.8
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    • pp.124-134
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    • 2017
  • The purpose of this study was to investigate the MBTI personality type, emotional intelligence, and stress coping strategies of nursing students and to examine the influence of these factors on their level of college adjustment.The subjects were 267 nursing students who are located in Chonnam city. The survey was conducted from March 6th to March 31st, 2017. The level of college adjustment showed a close correlation with the emotional intelligence(r=.29, p<.001) and stress coping strategies(r=.27, p<.001), and the level of MBTI personality type was not statistically insignificant in the nursing students. It was found that the effect of the emotional intelligence and stress coping strategies on the college life adjustment was 17.5%(F=8.03, p<.001). According to the results of this study, it is necessary to develop a systematic management program to integrate the educational curriculum and extra-curricular activities(in order to enhance the) emotional intelligence and stress coping strategies in nursing students. In addition, the type of psychological function and psychological temperament of the MBTI personality type were found not to have influenced the level of college adaptation of the nursing students and this should be confirmedin a large scale sample.

Influence of Emotional Intelligence, Communication Competence, and Interpersonal Competence on Adaptation to College Life in Nursing Students (간호대학생의 감성지능, 의사소통능력, 대인관계유능성이 대학생활적응에 미치는 영향)

  • Young-Hee Cho;Eun-Mi Lee;Yun-Jeong Oh
    • Journal of Industrial Convergence
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    • v.21 no.11
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    • pp.117-124
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    • 2023
  • This study is a descriptive research study to determine the relationship between nursing students' emotional intelligence, communication competence, interpersonal competence, and adaptation to college life, and to identify factors that adaptation to college life. The subjects of the study were 173 nursing students attending a university in G City, and data collection was conducted from September 10 to October 10, 2022 Descriptive statistics, t-test, ANOVA, Pearson correlation coefficient, and multiple regression analysis were performed on the collected data using SPSS 25.0 program. The results showed a significant positive correlation between emotional intelligence(r=.760, p<.001), communication competence(r=.600, p<.001), and interpersonal competence(r=.451, p<.001). Factors that have a significant impact on adaptation to college life include emotional intelligence(𝛽=.543, p<.001), communication competence(𝛽=.433, p=.001), and interpersonal competence(𝛽=.283, p<.031). The total explanatory power of these variables on adaptation to college life was 59.9%, and emotional intelligence was confirmed to be the main influencing factor.

Relationship between Smartphone Addiction and Sensory Processing Ability of Preschool Children (학령전기 아동의 스마트폰 중독과 감각처리능력과의 관계)

  • Kim, Chae-Hyeon;Kim, Kyeong-Mi;Chang, Moon-Young;Jung, Hyerim
    • The Journal of Korean Academy of Sensory Integration
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    • v.19 no.2
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    • pp.1-11
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    • 2021
  • Objective : The purpose of this study was to compare sensory processing ability by smartphone addiction levels in preschool children, and to investigate the correlation between smartphone addiction level and sensory processing ability within smartphone addiction group. Method : The subjects of this study were 324 persons, with 124 persons in the addiction group and 200 in the normal user group. Measurements in this study were a questionnaire about general characteristics of subject, smartphone addiction scale, and short sensory profile. Methods for the data analysis included descriptive statistics, independent t-test, Pearson correlation analysis of SPSS 22.0. Results : There was a significant difference in the total Short Sensory Profile (SSP) score and in all sub-domains between the addiction and normal use groups (p<0.05). In the smartphone addiction group, there was a negative correlation between the SSP total score (r=-.278), auditory filtering (r=-.293), visual/auditory sensitivity (r=-.393) and smartphone addiction level. Conclusion : This study confirmed that there was a difference in smartphone addiction and sensory processing ability between the preschool children addiction and normal use groups. It has been proven that there is an interrelationship between sensory processing ability and smartphone addiction in the addiction group. It is significant in that it provides basic data to prevent smartphone addiction.

Source Tracking of Particular Matters using Stable Isotope Analysis and Water Quality Characteristics in Gulpo and Anyang Stream, Han-River (한강수계 굴포천과 안양천에서 안정동위원소와 수질 특성을 이용한 입자성 물질의 기원 추적)

  • Hong, Jung-Ki;Im, Jong Kwon;Son, Ju Yeon;Noh, Hye-Ran;Yu, Soon-Ju;Lee, Bo-Mi
    • Journal of Korean Society on Water Environment
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    • v.36 no.2
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    • pp.116-124
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    • 2020
  • This study aims to identify the characteristics of organic matter and pollutant sources using water quality and stable isotope ratios (δ15N, δ13C) of the two inflow tributaries (Gulpo (GP) and Anyang (AY) streams). Water samples were analyzed in June and September 2018, and the results showed that the concentrations of nutrients, such as TN and NO3-N, were increased at GP4, which is located at the downstream of sewage treatment facilities(STFs). TOC and TN ratios showed a strong positive correlation (R2 = 0.77, p<0.01) at all points except for GP4. The results of GP's stable isotope ratio analysis do not appear to be a constant cluster compared to AY because GPs with large amounts of pollutants from the industry (metal processing companies, etc.) have less tributary, shorter waterway and significantly different external sources. This could be attributed to different sources of external inflow despite its smaller number of tributaries and shorter waterways than AY. In the first half of the year, the δ155N of GP4 was affected by discharge of STFs, while AY3 seemed to have an influence of tributary than the discharge of STFs. Consequently, using water quality, stable isotope ratio and C/N, the sources of contamination in two streams with different contaminants were identified and origin was estimated.

Identification and inhibiting effect of Lactobacillus salivarius the formation of plaque and the production of volatile sulfur compounds by anaerobic bacteria (치태형성과 혐기성 세균의 황화합물 생성에 대한 Lactobacillus salivarius의 억제효과 및 동정)

  • Kim, Mi-Hyung;Sun, Gem-Ju;Ahn, Yeon-Jun
    • Journal of Korean society of Dental Hygiene
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    • v.5 no.2
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    • pp.131-145
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    • 2005
  • There are normal inhabitants doing medically useful functions in the body. There are many kinds of bacteria performing specific functions in the oral cavity. Two strains of lactic add bacteria were isolated from normal inhabitants of children's oral cavity, which inhibited the production of volatile sulfur compounds by anaerobic bacteria. The authors identified the isolates by 16S rDNA partial sequencing. 1. Two isolates were Gram-positive bacilli and produced hydrogen peroxide. 2. When Streptococcus mutans was cultured in the media, the mean weight of formed artificial plaque on the orthodontic wires was $124.4{\pm}30.4$ mg, whereas being reduced to $5.2{\pm}2.0$ mg and $10.6{\pm}6.6$ mg in the media cultured with Streptococcus mutans and each isolate, respectively(p<0.05). 3. The number of viable cells of Streptococcus mutans was $3.4{\times}10^9$ per ml in the cultured solution, whereas those of Streptococcus mutans in the combined culture with each of isolates were $4.6{\times}10^8$ and $2.4{\times}10^8$ per ml. 4. The optical density was 1.286 in the supernatant of Fusoacterium nucleatum after vortexing for 30 minutes, whereas in the supernatant of combined Fusoacterium nucleatum and each isolate, they were reduced to 0.628 and 00497, which the percentages of coaggregation between them were 2904% and 57.8%, respectively. 5. The optical density of Fusoacterium nucleatum precipitate was 1.794 in the culture media containing cysteine and $FeSO_4$ being reduced to 1.144 and 0.915 in the coaggregated precipitates of Fusoacterium nucleatum and each isolate. 6. The similarity values of 16S rDNA sequence between each of isolates and Lactobacillus salivarius subsp. salicinius were 99.60% and 99.73%, respectively, meaning that isolates were Lactobacillus salivarius subsp. salicinius. These results indicated that two strains isolated from children's saliva, which inhibited the formation of plaque and the production of volatile sulfur compounds, were identified as Lactobacillus salivarius subsp. salicinius.

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Development of Near-Infrared Reflectance Spectroscopy (NIRS) Model for Amylose and Crude Protein Contents Analysis in Rice Germplasm (근적외선 분광광도계를 이용한 벼 유전자원 아밀로스 및 단백질 함량분석을 위한 모델개발)

  • Oh, Sejong;Lee, Myung Chul;Choi, Yu Mi;Lee, Sukyeung;Oh, Myeongwon;Ali, Asjad;Chae, Byungsoo;Hyun, Do Yoon
    • Korean Journal of Plant Resources
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    • v.30 no.1
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    • pp.38-49
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    • 2017
  • The objective of this research was to develop Near-Infrared Reflectance Spectroscopy (NIRS) model for amylose and protein contents analysis of large accessions of rice germplasm. A total of 511 accessions of rice germplasm were obtained from National Agrobiodiversity Center to make calibration equation. The accessions were measured by NIRS for both brown and milled brown rice which was additionally assayed by iodine and Kjeldahl method for amylose and crude protein contents. The range of amylose and protein content in milled brown rice were 6.15-32.25% and 4.72-14.81%, respectively. The correlation coefficient ($R^2$), standard error of calibration (SEC) and slope of brown rice were 0.906, 1.741, 0.995 in amylose and 0.941, 0.276, 1.011 in protein, respectively, whereas $R^2$, SEC and slope of milled brown rice values were 0.956, 1.159, 1.001 in amylose and 0.982, 0.164, 1.003 in protein, respectively. Validation results of this NIRS equation showed a high coefficient determination in prediction for amylose (0.962) and protein (0.986), and also low standard error in prediction (SEP) for amylose (2.349) and protein (0.415). These results suggest that NIRS equation model should be practically applied for determination of amylose and crude protein contents in large accessions of rice germplasm.

A Study on Migration of Ethylenediamine and Hexamethylenediamine from Polyamide Utensils (Polyamide 조리기구로부터 Ethylenediamine 및 Hexamethylenediamine의 이행에 관한 연구)

  • Eom, Mi-Ok;Kwak, In-Shin;Yoon, Hae-Jung;Jeon, Dae-Hoon;Choi, Hyun-Chul;Kim, Hyung-Il;Sung, Jun-Hyun;Park, Na-Young;Kim, So-Hee;Lee, Young-Ja
    • Journal of Food Hygiene and Safety
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    • v.25 no.1
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    • pp.36-42
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    • 2010
  • The method for the determination of ethylenediamine (EDA) and hexamethylenediamine (HMDA) in food simulants was developed, and migration amounts of these compounds was monitored for 124 polyamide (PA) utensils. The diurethane derivatives of EDA and HMDA, which produced by reaction with ethyl chloroformate, were analyzed by using gas chromatograph (GC)/flame ionization detector (FID) and GC/mass spectrometer (MS). The developed method was validated with $0.3\;{\mu}g/mL$ of limit of detection (LOD) for EDA and $0.1\;{\mu}g/mL$ of LOD for HMDA, > 0.999 of linearity($r^2$) and > 88% of recovery. The EDA was detected 1.31 and $02.06\;{\mu}g/mL$ for 2 samples in water. The HMDA was detected $0.29\;-\;0.93\;{\mu}g/mL$ for 3 samples in 20% ethanol and $0.26\;-\;0.44\;{\mu}g/mL$ for 10 samples in n-heptane. These migration levels were below the specific migration limits (SML) of $12\;{\mu}g/mL$ and $2.4\;{\mu}g/mL$ for EDA and HMDA established in EU.

Construction of Database System on Amylose and Protein Contents Distribution in Rice Germplasm Based on NIRS Data (벼 유전자원의 아밀로스 및 단백질 성분 함량 분포에 관한 자원정보 구축)

  • Oh, Sejong;Choi, Yu Mi;Lee, Myung Chul;Lee, Sukyeung;Yoon, Hyemyeong;Rauf, Muhammad;Chae, Byungsoo
    • Korean Journal of Plant Resources
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    • v.32 no.2
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    • pp.124-143
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    • 2019
  • This study was carried out to build a database system for amylose and protein contents of rice germplasm based on NIRS (Near-Infrared Reflectance Spectroscopy) analysis data. The average waxy type amylose contents was 8.7% in landrace, variety and weed type, whereas 10.3% in breeding line. In common rice, the average amylose contents was 22.3% for landrace, 22.7% for variety, 23.6% for weed type and 24.2% for breeding line. Waxy type resources comprised of 5% of the total germplasm collections, whereas low, intermediate and high amylose content resources share 5.5%, 20.5% and 69.0% of total germplasm collections, respectively. The average percent of protein contents was 8.2 for landrace, 8.0 for variety, and 7.9 for weed type and breeding line. The average Variability Index Value was 0.62 in waxy rice, 0.80 in common rice, and 0.51 in protein contents. The accession ratio in arbitrary ranges of landrace was 0.45 in amylose contents ranging from 6.4 to 8.7%, and 0.26 in protein ranging from 7.3 to 8.2%. In the variety, it was 0.32 in amylose ranging from 20.1 to 22.7%, and 0.51 in protein ranging from 6.1 to 8.3%. And also, weed type was 0.67 in amylose ranging from 6.6 to 9.7%, and 0.33 in protein ranging from 7.0 to 7.9%, whereas, in breeding line it was 0.47 in amylose ranging from 10.0 to 12.0%, and 0.26 in protein ranging from 7.0 to 7.9%. These results could be helpful to build database programming system for germplasm management.

Physicochemical Properties and Microbial Analysis of Korean Solar Salt and Flower of Salt (한국산 꽃소금과 천일염의 이화학적 특성 및 미생물 분석)

  • Lee, Hye Mi;Lee, Woo Kyoung;Jin, Jung Hyun;Kim, In Cheol
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.42 no.7
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    • pp.1115-1124
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    • 2013
  • The present study was conducted to ensure the diversity of domestic solar salt by analyzing the composition and microbiological characteristics of solar salt (from Docho island: DS) and the flower of salt produced in different Korean salt flats (Sinui island: SF, Bigum island: BF, and Docho island: DF). The analyses showed that the moisture content of the three types of flower of salt and solar salt ranged from 10.54~13.82% and NaCl content ranged from 78.81~84.61%. The mineral content of those salts ranged from 3.57~5.51%. The content of insoluble matter in these salts was $0.01{\pm}0.00{\sim}0.05{\pm}0.00%$. The sand content of these salts was $0.01{\pm}0.01{\sim}0.03{\pm}0.01%$. By Hunter's color value analysis, the color of the flower of salt was brighter and whiter than solar salt. The salinity of the flower of salt was a little higher than solar salt as well. The magnesium and potassium ion content of DF was $9,886.72{\pm}104.78mg/kg$ and $2,975.23{\pm}79.73mg/kg$, respectively, which was lower than the content in SF, BF, and DS. The heavy metal content of all salts was acceptable under the Korean Food Sanitation Law. The flower of salt was confirmed to be sweeter and preferable to solar salt. More than 80% of the solar salt crystals were 2~3 mm in size, whereas crystals from the flower of salt were 0.5~2 mm in size. The bacterial diversity of DF and DS were investigated by culture and denaturing gradient gel electrophoresis (DGGE) methods. The number of cultured bacteria in flower of salt was approximately three times more than solar salt. By DGGE analysis, major microbes of DF were Maritimibacter sp., Cupriavidus sp., and unculturable bacteria, and those of DS were Cupriavidus sp., Dunalidella salina and unculturable bacteria. The results of DGGE analysis showed that major microorganisms in solar salts were composed of unidentified and unculturable bacteria and only a few microorganisms were culturable.