• Journal of Pharmaceutical Investigation
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• v.28 no.1
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• pp.1-5
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• 1998

The effect of synthetic polymer membranes on the permeation rate of dideoxynucleoside-type anti-HIV drugs through hairless rat skin was studied using ethylene/vinyl acetate copolymer (EVA) and ethylene/methyl acrylate copolymer (EMA) membranes fabricated by solvent casting method. In vitro skin permeation kinetics study of DDC (2',3'-dideoxythymidine), DDI (2',3'-dideoxyinosine) and AZT (3'-azido-3'-deoxythymidine) across the (membrane/skin) composite was conducted for 24 hours at $37^{\circ}C$ using the Valia-Chien skin permeation system. The results showed that skin permeation rate of each drug across the (skin/membrane) composite was mainly dependent on the property of the membrane. Proper selection of the polymeric membrane which resembles hydrophilicity/lipophilicity of the delivering drug was important in controlling the skin permeation rate.

• KOREAN JOURNAL OF PACKAGING SCIENCE & TECHNOLOGY
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• v.9 no.1
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• pp.1-6
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• 2003

The partitioning behavior of five printing ink solvents was studied in various cookie ingredients before and after baking which had different water content and different structure. Solvents were ethyl acetate, hexane, isopropanol, methyl ethyl ketone, and toluene which represent different characteristic functional groups. Gas chromatography (G.C.) was used to measure partitioning values at $25^{\circ}C$ on each raw and baked cookie ingredients. Baking condition of cookie ingredients was $260^{\circ}C$ for 10 min. In cookie ingredients, decreases in water content generally affected Kp of polar solvents, but did not affect that of the non-polar solvents. However, as water content decreased in the cookie ingredient, the Kp of the non-polar and polar solvents showed mixed results.

• Preventive Nutrition and Food Science
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• v.3 no.2
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• pp.119-122
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• 1998

Pumpkin venegar was produced using autoclaved pumpkin juice by fermenting with cetobacter starter and ethanol at the ratio of 4 % and 10% to the volume of pumpkin juice, respectivley. Fermentation was carried out at 2$0^{\circ}C$ for 14 days followed by aging at 1$0^{\circ}C$ for 14 days. Flavor components of pumpkin vinegar was determined using GC/MS. Identified components, were 2 aldehydes (4.74%), 5 acohols (30.06%), 4 ketones (8.99%), 4 acids (16.39%), 5 alkanes (11.10%), 11 miscellaneous compounds (27.01%) and 9 unknown compounds (1.71%). Pumpkin vinegar showed very similar flavor characteristics to those of conventional wien vinegar and sherry wine vinegar in particular , acetioin, methyl acetate, and butanoic acid were typical volatile components of these three kinds of vinegar. Pumpkin vinegar showed possiblity to compete with European wine vinegar.

• Journal of the Korean Society of Food Science and Nutrition
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• v.42 no.12
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• pp.2042-2048
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• 2013

This research was carried out to compare the physicochemical characteristics of Korean traditional spirits brewed with Phellinus linteus by different Nuruks. The spirits were produced by atmospheric distillation of rice mash added with 1~5% P. linteus and fermented by traditional and improved Nuruks. Traditional Nuruk facilitated more alcohol production than improved Nuruk during the initial fermentation time. The alcohol contents of rice mash with P. linteus ranged from 15.8 to 18.9%. Predominant volatile compounds were i-amyl alcohol of fusel oils and ethyl acetate of ester compounds in P. linteus added spirits. The spirits prepared by traditional Nuruk showed higher contents of i-butanol, n-butanol, and i-amyl alcohol than those by improved Nuruk. More ethyl acetate was produced in the spirits prepared by improved Nuruk than those by traditional Nuruk. The contents of methyl alcohol were less than 50 mg/L in P. linteus added spirits.

• Applied Biological Chemistry
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• v.51 no.3
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• pp.212-218
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• 2008

This study was performed to determine the antimutagenic and anticancer effects of ethanol extract of buckwheat sprout using Ames test and SRB assay, respectively. An ethyl acetate fraction (200 ${\mu}/plate$) from the ethanol extract of buckwheat sprout showed inhibition rate of 80.6% against the mutagenesis induced by N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) in Salmonella typhimurium TA100 strain. Also the ethyl acetate fraction (200 ${\mu}/plate$) showed higher antimutagenic activity than other fractions against the mutagenesis induced by 4-nitroquinoline-1-oxide (4NQO) in Salmonella typhimurium TA98 and TA100. In addition, the ethyl acetate fraction (200 ${\mu}/plate$) showed high antimutagenic effect of 80.9% and 85.9% against the mutation of TA98 and TA100 strains induced by 3-amino-1,4-dimethyl-5H-pyrido-(4,3-b)indol (Trp-P-1), respectively. The cytotoxic effects of each solvent fraction from the ethanol extract of buckwheat sprout against human cancer cell lines including lung carcinoma (A549), gastric carcinoma (AGS), breast adenocarcinoma (MCF-7), hepatocellular carcinoma (Hep3B), and colon adenocarcinoma (Colo 205) were investigated. The ethyl acetate fraction of buckwheat sprout ethanol extract at the concentration of 1.0 mg/ml showed strong cytotoxic activities of 70.3, 94.8, 79.6, 82.3, and 73.2% against A549, AGS, MCF-7, Hep3B and Colo 205 cancer cell lines, respectively.

• Journal of the Korean Wood Science and Technology
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• v.44 no.3
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• pp.337-349
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• 2016

In this study, an analytical method to evaluate the quality of isoflavonoid compounds purified and isolated from the fruits of Cudrania tricuspidata was developed and validated using Ultra Performance Liquid Chromatography (UPLC). The fruits of C. tricuspidata were extracted with methanol and further fractioned with n-hexane, ethyl acetate and water. The resulting ethyl acetate extract separated into four isoflavonoid compounds by a combination of silica gel and sephadex LH-20 column chromatography. The structures of the compounds were elucidated as alpinumisoflavone, 6,8-diprenyl genistein, 6,8-diprenyl orobol, 4'-O-methylalpinumisoflavone by various techniques such as UV-Vis, ESI-MS, $^1H\;NMR$ and $^{13}C\;NMR$ spectroscopy. Finally, a method to characterize the compounds was developed by using the UPLC equipped with a $C_{18}$ column and a gradient mobile phase system consisting of 2% acetic acid in water (solvent A) and 2% acetic acid in methanol (solvent B). The developed method was validated with the parameters such as selectivity, linearity, limit of detection, limit of quantitation, accuracy, and precision, which are defined by the ICH (International Conference on Harmonization). Using the validated method, the compounds in the fruits harvested in different months were also quantitatively analyzed. We propose this approach this approach can readily be utilized as an efficient evaluation method to quantify the extracts of C. tricuspidata.

• Archives of Pharmacal Research
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• v.18 no.2
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• pp.90-99
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• 1995

The effect of protein kinase C inhibitors, sturosporine and 1-(5-isoquinolinyl sulfonyl)-2-methyl piperazine(H7) on in vitro differentiation of erythroid progenitor cells which were isolated from spleens of mice infected with the anemia-inducing strain of Friend virus were examined. Erythropoietin-mediated differentitation of erythroid progenitor cells, as determined by the incorporation of $^{59}Fe$ into protoporphyrin, was inhibited by staurosporine and H7 in a concentration -dependent manner. Scatchard analysis of the $^3H-phorbol-12$, 13-dibutyrate binding to erythroid progenitor cells revealed that at the high affinity sites the dissociation constant was 22nM and the maximum number of $^3H-phorbol-12$, 13-dibutyrate binding to erythroid progenitor cells revealed that at the high affinity sites the dissociation constant was 22nM and the maximum number of $^3H-phorbol-12$, 13-dibutyrate binding sites per cell was approximately $3.7\times10^5$. Cytosonic protein kinase C was isolated from erthroid progenitor cells and then purified by sequential column chromatogrphy. Two isoforms of protein kinase C were found. Photoaffinity labeling of the purified protein kinase C samples with $^3H-phorbol-12$12-myristate 13-acetate followed by analysis of SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and autofluorography showed radiolabeled 82-KDa pepticles. Rediolabeling of the 82-KDa peptides with $^3H-phorbol-12$myristate 13-acete was almost completely blocked by excess unlabeled phorbol 12-myristate 13-acetate was almost 12-muristate 13-acetate-promoted phosphorylation with the puyrified protein kinase C samples showed that the phosphorylation of 82-KDa peptides was increased as the concentration of phorbol 12-myristate 13-acetate was increased from $10^{-8}M{\;}to{\;}10^{-4}$M. In light of the findings that erythroid progenitor cells possessed an abundance of protein kinase C and that stauroporine and H7 inhibited erythroid differentiation, it seemed likely that protein kinase C would play a role in the erythroid progenitor cell development.

• Journal of Soil and Groundwater Environment
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• v.14 no.2
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• pp.26-32
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• 2009

In this study, the degradation of BTEX (benzene, toluene, ethylbenzene, xylene) was tested in both (Fe$^{3+}$+chelating agent)/H$_2$O$_2$system [Fe(III) 1 mM, oxalate 6 mM, H$_2$O$_2$ 3%, and pH 6] and UV/(Fe3++ chelating agent)lHzOz system [UV dose 17.4 kWhlL, Fe(III) 1mM, oxalate 6 mM,H$_2$O$_2$ 1%, and pH 6]. The types of chelating agents used in experiments were catechol, NTA, gallic, acetyl acetone, succinic, acetate, EDTA, citrate, malonate, and oxalate and the optimum chelating agent for BTEX degradation was determined. The results showed that acetate was the optimum chelating agent for BTEX degradation in both (Fe$^{3+}$+chelating agent)/H$_2$O$_2$ and UV/(Fe$^{3+}$+chelating agent)/H$_2$O$_2$ system, and UV radiation enhanced the degradation of BTEX with any types of chelating agents. Moreover, UV/(Fe$^{3+}$+chelating agent)/H$_2$O$_2$ system, which chelating agent was acetate, removed effectively mixtures of BTEX and MTBE (methyl tert-butyl ether) when the concentration of both BTEX and MTBE was 200 mg/L, respectively. In this system, BTEX was degraded completely and 85% of MTBE was degraded at the reaction time of 180 min. Therefore, UV/((Fe$^{3+}$+chelating agent)/H$_2$O$_2$ system with acetate as a chelating agent removed not only BTEX but also BTEX and MTBE, effectively.

• Analytical Science and Technology
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• v.15 no.1
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• pp.80-86
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• 2002

The simultaneous analysis of the odorous compounds designated by law in Korea and Japan was examined with the thermal desorber gas chromatography-mass spectrometry using one column. The approximate concentrations of trimethyl amine, acetaldehyde, methyl mercaptan and dimethyl sulfide were estimated. Styrene, dimethyl disulfide, propionaldehyde, n-butyl aldehyde, i-butyl aldehyde, n-valeraldehyde, i-valeraldehyde, ethyl acetate, toluene, xylene, methyl i-butyl ketone and i-butanol were detected at concentrations of the detection limits of their threshold values. As a typical example of simultaneous analysis of the odorous compounds, the volatile organic compounds emitted from compost procedure of food waste were concentrated and analyzed with thermal desorber/GC/MSD, and major malodorous compounds were estimated from the concentrations and threshold values of the detected components. From the result of analysis, 34 compounds were confirmed and among them, trimethyl amine, i-valeraldehyde, methyl mercaptan, methyl allyl sulfide, dimethyl sulfide, acetaldehyde, ethanol, n-butyaldehyde were expected to attribute to the odor in order of strength.

• The Korean Journal of Pesticide Science
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• v.18 no.2
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• pp.104-114
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• 2014

Scale insects heavily damaged many agricultural crops and trees in urban and forest areas. This study was conducted to carry out alternative control methods for environmentally friendly control of scale insects. Hot water extract of Quisquali indica produced high mortality against the black pine bast scale, Matsucoccus thunbergianae in previously experiment. Methanol extract obtained from fruit of Q. indica was successively fractionated using hexane, chloroform, ethyl acetate, buthanol and water which were examined by exposing for their insecticidal activity against four Cocoidea species (Eriococcus lagerstroemiae, Ceroplastes japonicus, Crisicoccus pini and Planococcus citri) in laboratory. Hexane fraction gave the highest insecticidal activity against scale insect pest. GC-mass analysis confirmed that the main compounds of Q. indica were alpha-pinene, methyl palmitate, eugenol, methyl myristate, phthalic acid mono(2-ethylhexyl)ester and palmitic acid. Among those compounds, ${\alpha}$-pinene was included 10 hexane subfractions. $LC_{50}$ of ${\alpha}$-pinene was 0.0114 ppm against 2nd instar of E. lagerstroemiae. These results suggest that Q. indica extracts might be used as an alternative control agent of scale insects.