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Antimutagenic and Cytotoxic Effects of an Ethanol Extract of Buckwheat Sprout  

Cui, Cheng-Bi (Department of Agriculture, Yanbian University)
Lee, Eui-Yong (R&D Team, Bakery Plant, CJ Corp.)
Ham, Seung-Shi (School of Biotechnology and Bioengineering, Kangwon National University)
Lee, Deuk-Sik (Department of Foodservice Industry, Hanzhong University)
Publication Information
Applied Biological Chemistry / v.51, no.3, 2008 , pp. 212-218 More about this Journal
Abstract
This study was performed to determine the antimutagenic and anticancer effects of ethanol extract of buckwheat sprout using Ames test and SRB assay, respectively. An ethyl acetate fraction (200 ${\mu}/plate$) from the ethanol extract of buckwheat sprout showed inhibition rate of 80.6% against the mutagenesis induced by N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) in Salmonella typhimurium TA100 strain. Also the ethyl acetate fraction (200 ${\mu}/plate$) showed higher antimutagenic activity than other fractions against the mutagenesis induced by 4-nitroquinoline-1-oxide (4NQO) in Salmonella typhimurium TA98 and TA100. In addition, the ethyl acetate fraction (200 ${\mu}/plate$) showed high antimutagenic effect of 80.9% and 85.9% against the mutation of TA98 and TA100 strains induced by 3-amino-1,4-dimethyl-5H-pyrido-(4,3-b)indol (Trp-P-1), respectively. The cytotoxic effects of each solvent fraction from the ethanol extract of buckwheat sprout against human cancer cell lines including lung carcinoma (A549), gastric carcinoma (AGS), breast adenocarcinoma (MCF-7), hepatocellular carcinoma (Hep3B), and colon adenocarcinoma (Colo 205) were investigated. The ethyl acetate fraction of buckwheat sprout ethanol extract at the concentration of 1.0 mg/ml showed strong cytotoxic activities of 70.3, 94.8, 79.6, 82.3, and 73.2% against A549, AGS, MCF-7, Hep3B and Colo 205 cancer cell lines, respectively.
Keywords
anticancer; antimutagenicity; buckwheat sprout; cytotoxicity; inhibition;
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Times Cited By KSCI : 9  (Citation Analysis)
Times Cited By Web Of Science : 0  (Related Records In Web of Science)
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1 Choi, B. H., Kim, S. K., Park, K. Y. and Park, R. Y. (1995) Agronomic character and productivity of genetic resources in buckwheat (Fagopyrum esculentum Moench) (in Korean). RDA, J. Agric. Sci. 37, 221-227
2 Shibatr, S., Imal, T., Ghikubu, S. and Miyyahara, T. (1979) The composition of buckwheat flour of various varieties and cultivated at various periods. Rept. Nalt. Food Res.Inst. 34, 1-7
3 Lee, J.S., Lee, M.H., Chang, Y.K., Ju, J.S. and Son, H.S. (1995) Effects of buckwheat diet on serum glucose and lipid metabolism in NIDDM. Korean J. Nutr. 28, 809-816
4 Martin, A. and Martin, C. (1997) Comparision of 5 microplate colorimetric assay for in vitro cytotoxicity testing and cell proliferation assays. Cytotechnology 11, 49-54   DOI
5 Kim, S.H., Lee, E.Y. and Ham, S.S. (2007) Antioxidation and antigenotoxic effects of buckwheat sprout extracts. J. Korean Soc. Food Nutr. 36, 955-959   과학기술학회마을   DOI
6 Ham, S.S., Choi, K.P., Choi, Y.S. and Lee, S.Y. (1994) Studies on antimutagenic and lipotropic action of flavonoids of buckwheats. J. Korean Soc. Food Nutr. 23, 698-703   과학기술학회마을
7 Lee, J.S., Soo, S.S., Maeng, Y.S., Chang, Y.K. and Ju, J.S. (1994) Effects of buckwheat on organ on weight, glucose and lipid metabolism in streptozotocin-induced diabetic rats. Korean J. Nutr. 27, 819-827
8 Ham, S.S., Choi, G.P., Choi, Y.S. and Lee, S.Y. (1994) Antimutagenic study of buckwheat leaves ethanol extract. J. Korean Soc. Food Sci. 23, 698-703
9 Hertog, M.G., K romhout, D., Aracanis, C., Blackburn, H., Buzina, E., Fidanza, F., Giampaoli, S., Jansen, A., Menotti, A., Nedeljkovic, S., Pekkarinen, M., Simic, B.S., Toshima, H., Feskens, E.J.M., Hollman, P.C.H. and Katan, M.B. (1995) Flavnoid intake and longterm risk of coronary heart disease and cancer in the countries study. Arch. Intern. Med. 155, 381-386   DOI
10 Kwak, C.S., Lim, S.J., Kim, S.A., Park, S.C. and Lee, M.S. (2004) Antioxidative and antimutagenicity effects of Korean buckwheat, sorghum, millet and Job's tears. J. Korean Soc. Food Sci. Nutr. 33, 921-929   과학기술학회마을   DOI
11 Kwon, T.B. (1994) Changes in rutin and fatty acids of buckwheat during germination. J. Food Nutr. 7, 124-127   과학기술학회마을
12 Yoo, K.H., Kim, S.H., Ham, Y.A., Yoo, S.J., Oh, H.T. and Ham, S.S. (2006) Antimutagenic and cytotoxic effects of Fagopyrum esculentum Moenech noodles extracts. J. Korean Soc. Food Nutr. 35, 1291-1296   과학기술학회마을   DOI
13 He, J., Klag, M.J., Whelton, P.K., Mo, J.P., Chen, J.K., Qian, M.C., Mo, P.S. and He, G.Q. (1995) Oats and buckwheat intakes and cardiovascular disease risk factors in buckwheat intakes and cardiovascular disease risk factors in an ethnic minority of China. Am. J. Clin. Nutr. 61, 366-372   DOI
14 Ikeda, K., Arioka, K., Fujii, S., Kusano, T. and Oku, M. (1984) Effect on buckwheat protein quality of seed germination and changes in trypsin inhibitor content. Cereal Chem. 61, 236-240
15 Matsuhashi, T. (1984) Composition of buckwheat and buckwheat powder. New Food Industry 26, 54-58
16 Griffith, J.Q., Couch, J.F. and Lindauer, M.A. (1995) Effect of rutin on increased capillary fragility in man. Proc. Soc. Exp. Bio. Med. 55, 228-229
17 Ham, S.S., Lee, S.Y., Choi, M. and Hwang, H.J. (1998) Antimutagenicity and cytotoxicity effects of woorimil wheat flour extracts added with wila herb and seaweed powder. J. Korean Soc. Food Sci. Nutr. 27, 1177-1182   과학기술학회마을
18 Maron, D.M. and Ames, B.N. (1983) Revised methods for the Salmonella mutagenicity test. Mutation Res. 113, 173-215   DOI
19 Matsubara, Y., Kumamoto, H., Lizuka, Y., Murakami, T., Okamoto, K., Miyake, H. and Yokoi, K. (1995) Structure and hypotensive effect of flavonoid glycosides in Citrus ubshiu peelings. Agric. Biol. Chem. 49, 900-905
20 Lee, J.S., Park, S.J., Sung, K.S., Han, C.K., Lee, M.H., Jung, C.W. and Kwon, T.B. (2000) Effects of germinated-buckwheat on blood pressure, plasma glucose and lipid levels of spontaneously hypertensive rats. Korean J. Food Sci. Technol. 32, 206-211   과학기술학회마을
21 Keli, S.O., Hertog, M.G.K., Freskens, E.J.M. and Kromhout, D. (1996) Dietary flavonoids, antioxidant vitamins, and incidence of stroke: The Zutpen study. Arch. Intern. Med. 154, 637-642
22 Maeng, Y.S., Park, H.K. and Kwon, T.B. (1990) Analysis of rutin contents in buckwheat foods. Korean. J. Food Sci. Technol. 22, 732-737   과학기술학회마을
23 Lee, S. Y. (1997) History of buckwheat, Buckwheat 1, 3-6
24 Lee, M.H., Woo, S.J., Oh, S.K. and Kwon, T.B. (1994) Changes in contents and composition of dietary fiber during buckwheat germination. J. Food Nutr. 7, 274-283   과학기술학회마을