• Natural Product Sciences
• /
• 제13권3호
• /
• pp.207-213
• /
• 2007

Scutellaria baicalensis is known as a herbal medicine with anti-inflammatory and anti-oxidative activities. However, effect of Scutellaria baicalensis on lupus pathogenesis that is characterized by overproduction of proinflammatory cytokines and abnormalities in regulation, function, and interaction of immune cells remains unclear. We investigated effects of Scutellariae radix methanol extract (SBMeOH) on the production of proinflammatory cytokines and abnormal activation of T cells in vitro in pristane-induced lupus BALB/c mice. These results demonstrated that SBMeOH significantly decreased the LPS-stimulated production of $TNF-{\alpha}$, IL-6, and IL-10 by splenic and peritoneal macrophages and IL-6 and IL-10 by splenocytes from pristane-induced lupus mice. SBMeOH significantly downregulated the Con A-stimulated overproduction of IL-6, IL-10, and $IFN-{\gamma}$ by splenocytes from pristane-induced lupus mice. Also, SBMeOH significantly attenuated the Con A-induced expression of CD4+ T cells and CD69+CD4+ T cells but not CD8+ T cells in pristane-induced lupus mice. Our findings indicate that SBMeOH may ameliorate lupus pathogenic inflammation and autoimmunity via downregulation of proinflammatory cytokine production and abnormal activation of T cells.

• Bulletin of the Korean Chemical Society
• /
• 제34권5호
• /
• pp.1407-1413
• /
• 2013

A new compound, perforaphenonoside A (1), along with 11 known compounds (2-12) were isolated from a methanol extract of adventitious roots of Hypericum perforatum. Their chemical structures were elucidated using chemical and physical methods as well as comparison of NMR and mass spectral data with previously reported data. Their inhibition of NF-${\kappa}B$ and activation of PPAR was measured in HepG2 cells using a luciferase reporter system. Among the compounds 3, 6, 7 and 12 inhibited NF-${\kappa}B$ activation stimulated by TNF${\alpha}$ in a dose-dependent manner, with $IC_{50}$ values ranging from 0.85 to $8.10{\mu}M$. Moreover, compounds 1-3, 7, 11 and 12 activated the transcriptional activity of PPARs in a dose-dependent manner, with $EC_{50}$ values ranging from 7.3 to $58.7{\mu}M$. The transactivational effects of compounds 1-3, 7, 11 and 12 were evaluated on three individual PPAR subtypes. Among them, compound 2 activated $PPAR{\alpha}$ transcriptional activity, with 153.97% stimulation at $10{\mu}M$, while compounds 1, 2 and 11 exhibited transcriptional activity of $PPAR{\gamma}$, with stimulation from 124.76% to 126.91% at $10{\mu}M$.

• 동의생리병리학회지
• /
• 제30권3호
• /
• pp.150-156
• /
• 2016

Extract of Broussometia kazinoki Rhizodermatis has been traditionally used for geopoong, diuresis, hwalhyeol. In the present study, the apoptotic effect of methanol extract of Broussometia kazinoki (MBK) were investigated. Cell viability of A549 cells was measured by MTT assay. Apoptosis-related protein and MAPK protein levels were measured by Western blot. Chromatin condensation of A549 cells was stained with DAPI. MBK inhibited cell proliferation of A549 cell. Based on DAPI staining, MBK-treated cells manifested nuclear shrinkage, condensation and fragmentation. Treatment of A549 cells with MBK resulted in activation of the caspase-3, -8, -9 and cleavage of poly ADP-ribose polymerase (PARP). In the upstream, MBK increased the expressions Bax and Bak, decreased the expression of Bcl-2, and augmented the Bax/Bcl-2 ratio. MBK-induced apoptosis was accompanied by sustained phosphorylation of JNK, p38 MAPK and apoptosis signal-regulating kinase (ASK)-1. These results suggest that MBK induced apoptosis in A549 cells through Bcl-2 family protein-mediated mitochondria/caspase-3 dependent pathway. In addition, MBK increased the activation of ASK-1, which are critical upsteam signals for JNK/p38 MAPK activation in A549 cancer cells.

• 생명과학회지
• /
• 제25권6호
• /
• pp.638-645
• /
• 2015

본 연구는 ice plant (Mesembryanthemum crystallinum)의 생리활성을 분석하여 기능성 식품으로서의 가능성을 조사하였다. Ice plant는 아프리카와 서유럽, 북미, 남미 그리고 호주에서 서식하는 다육식물로 항당뇨, 항산화, 지질 대사 활성에 효능이 있다고 알려져 있다. Ice plant를 methanol (MCM), ethanol (MCE), 열수(MCHW), 열수 추출 후 methanol (MCHM)로 추출하여, mushroom tyrosinase, pancreatic lipase, DPPH free radical 소거 활성, NO 생성, α-glucosidase에 대한 저해 활성을 측정 하였다. MCM, MCE, MCHW, MCHM 추출물의 추출 수율은 0.37%, 0.33%, 0.5%, 0.07%로 나타났다. MCHW 추출물은 DPPH free radical 소거능에 높은 효과를 보였고, MCM, MCE 추출물은 lipase 저해 활성, α-glucosidase 저해 활성, NO 생성 저해활성을 보였다. 그러나 mushroom tyrosinase 저해 활성은 모든 추출물에서 매우 낮았다. 이러한 결과를 통해 ice plant는 항산화 물질로서 비만, 당뇨, 염증 치료용 기능성 식품으로서의 가능성이 매우 높음을 확인할 수 있었다.

• 한국식품영양과학회지
• /
• 제34권8호
• /
• pp.1136-1142
• /
• 2005

본 연구에서는 비교적 가격이 저렴하고 부작용이 없으면서 효능이 우수한 노화 억제 관련 생리 기능성 제품을 개발하고자 먼저 다양한 종류의 약용식물을 대상으로 물과 메탄올 추출물을 제조한 후 이들의 항산화 활성, SOD 활성과 elas-tase저해 활성을 측정하여 우수 약용식물을 선발한 후 이들로부터 노화 억제 관련 생리 활성 물질들의 추출 최적 조건을 검토하였다. 124종의 약용식물에 대한 물과 메탄올 추출물 가운데 항산화 활성은 도인(Prunus persica)의 메탄올 추출물이 98.4$\%$로 가장 높았고, SOD 활성은 구기자(Lycium chinense)의 물 추출물이 197$\%$로, elastase 저해활성은 상엽(Morus alba) 메탄올 추출물이 41$\%$로 가장 높았다 또한 항산화물질은 도인(Prunus persica)을 메탄올로 1 :20 현탁시킨 후 40$^{\circ}C$, 12시간 추출하였을 때 가장 많이 추출되었고 SOD 활성 물질은 구기자(Lycium chinense)를 1:30의 물로 30$^{\circ}C$, 12시간, elastase 저해 물질은 상엽 (morus alba)을 메탄올로 50$^{\circ}C$에서 12시간 진탕시켰을 때 가장 많이 추출되었다.

• 한국식품영양과학회지
• /
• 제39권3호
• /
• pp.356-362
• /
• 2010

동결건조된 솔잎(Pinus densiflora needles)으로부터 냉수(PD-CW), 열수(PD-HW)와 메탄올추출물(PD-M)을 분획하여 in vitro에서 Peyer's patch를 경유한 장관면역 활성을 측정한 결과, PD-M 획분에서 유력한 골수세포 증식활성을 나타내었다. 메탄올 추출방법을 확립하기 위하여 MeOH 추출물을 균질화, 교반 또는 환류 등의 방법으로 조제하였을 때, 환류방법으로 조제된 MeOH 추출물에서 유의적(p<0.05)으로 가장 높은 장관면역 활성을 in vitro에서 확인할 수 있었다. 솔잎으로부터 분획된 PD-M을 마우스에 경구 투여하고 Peyer's patch를 분리하여 ex vivo에서의 장관면역 활성도 관찰하였다. 다양한 농도로 1주일 동안 PD-M을 경구투여 한 결과, 1.0 g/kg of BW/day의 용량으로 경구투여 한 C3H/He 마우스의 Peyer's patch로부터 얻은 세포배양 상등액에서 saline 대조군보다 2.5배의 높은 장관면역 활성을 보여주었다(p<0.05). 또한 다양한 농도로 경구투여 된 Peyer's patch 세포의 배양액을 이용하여 측정한 IL-6 생산능은 1.0 g/kg of BW/day의 용량에서 1.13배로 증가하였으나 GM-CSF는 처리 용량에 따라 유의적인 값을 보이지는 않았다. 이러한 결과는 PD-M의 경구투여가 Peyer's patch 세포로부터 GM-CSF와 IL-6 등의 조혈세포 증식인자의 분비를 촉진함을 나타내는 것이며, 이러한 cytokine들이 골수세포 증식의 조절인자로서 작용함을 보여주는 것으로 생각한다.

• 한국패류학회지
• /
• 제29권3호
• /
• pp.251-258
• /
• 2013

본 연구에서는 굴, Crassostrea gigas의 계획적인 인공종묘생산 및 우량형질의 정자를 보존하기 위하여 냉동보존 시 적정 결빙억제제 (cryprotective agent, CPA) 및 농도를 알아보고자, CPA 종류 및 농도별 독성 및 냉동보존 효과를 파악하고 해동 후 정자의 세포 손상을 조사하였다. CPA 종류 및 농도별 침지시간에 따른 굴 정자의 독성평가를 실시한 결과, 생존율 및 운동성은 DMSO가 가장 좋았으며, 다음으로 EG, glycerol, Methanol 순이었다. 희석액으로 여과해수를 사용하여 CPA 종류 및 농도별 냉동보존 결과, 15% DMSO로 냉동보존 한 정자의 생존율 및 운동성이 가장 높았다. 굴의 냉동/해동 정자를 주사전자현미경으로 관찰한 결과, DMSO, EG, methanol, glycerol 순으로 세포 손상이 적었으며, 농도는 15, 20, 10, 5% 순이었다. 이상의 결과를 종합한 결과, 굴 정자의 냉동보존 시 여과해수를 희석액으로 사용할 경우 적정 CPA는 DMSO이며, 농도는 15%였다.

• 한국식품영양학회지
• /
• 제9권3호
• /
• pp.294-298
• /
• 1996

By the 505 chromotest which utilized Escherichia bolt PQ 37, Korean medicinal plants had been screened to Investigate the antimutagenic effect to aflatoxin B1(AFBl). Ikmocho(IMC, Leonurus sibiricus L.) was extracted with hot water. The extract was not found to be mutagenic in the Salmonella mutation test with or without metabolic activation, and the extract was showed to possess the antimutagenic properties towards AFB1-induced metation. The mutagenicity of AFB1 was inhibited by methanol soluble fracstion (IMC-MS) in dose-dependent. However, water-soluble fraction exhibited comutagenic activity. The greatest inhibitory effect of IMC-MS on AFB1 mutagenicity occurred when IMC-MS was first incubated, AFB1 followed by a second incubation with the cells and 59 mixture. Also lower inhibition was occurred when S9 mixtures were first incubated, with IMC-MS followed by a second incubation with AFBI. The results of the sequential incubation study support the probability that one mechanism of inhibition could involve the formation of chemical complex between IMC-MS and AFB1 rather than deactivation of S9 enzyme.

• Journal of Microbiology and Biotechnology
• /
• 제14권6호
• /
• pp.1157-1162
• /
• 2004

A well-holder type piezoelectric chloramphenicol (CAP) immunosensor which was prepared by binding an anti­CAP antibody to the chemisorbed monolayers of various thiol or sulfide compounds over the gold electrode surface of quartz crystals through a carboxyl-amine coupling procedure, using the activation with l-ethyl- 3-(3-dimethylarninopropyl)carbodiimide­HCl and N-hydroxysulfosuccinimide, was determined for its responses to CAP, CAP succinate, and water-soluble CAP. The reaction phase used in the well holder was 0.01 M phosphate buffer (pH 7.4), and the solvent for analyte dissolution varied according to the solubility of the individual analyte. The analyte detection which was indicated by a steady-state frequency shift was finished within 10 min, except for CAP dissolved in methanol. The responses of CAP succinate and water-soluble CAP in the reaction phase were very stable, while a minute fluctuation was found with CAP.

• 한국응용과학기술학회지
• /
• 제10권1호
• /
• pp.23-29
• /
• 1993

Transesterification reaction between dimethyl phthalate and ethylene glycol was kinetically investigated in the presense of various metal nitrate catalysts at $170^{\circ}C$. The reaction rates measured by the amount of distilled methanol from the reaction vessel. The transesterification reaction was carried out under the first order conditions respect to the concentration of dimethyl phthalate and catalyst, respectively. The over all order was 2nd. By Arrhenius plot, the activation energy was calculated as 17.4kcal/mole and 17.2kcal/mole on the transesterification reaction with zinc nitrate and lead nitrate, respectively. Apparent rate constant, k' was appeared linear about concentration of catalyst.