• 대한약학회:학술대회논문집
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• 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.1
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• pp.285.2-286
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• 2003

Analysis of illicit methamphetamine samples seized in Korea is discussed. The samples are extracted with the small portion of ethyl acetate under neutral conditions and the extracts are analyzed by GC-MS. Several impuritiy peaks are found in each chromatrogram. Eight compunds (1,2-Dimethyl-3-phenylaziridine, amphetamine oxime, ephedrine, N-formylmethamphetamine, N-acetylmethamphetamine, acetylephedrine, 3,4-dimethyl-5-phenyl-2-oxazolidone, methamphetamine dimer) are identified impurities in illicit methamphetamine and the identity of the impurity is conformed synthesis. (omitted)

• Journal of Acupuncture Research
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• 제26권1호
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• pp.39-47
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• 2009

Objectives : The mesolimbic dopamine system is believed to play a major role in the reinforcing effect and behavioral hyperactivity of abused drugs including methamphetamine. In the present study, the effect of acupuncture on methamphetamine-induced locomotor activity and c-Fos expression in the striatum and nucleus accumbens of rats were examined. Methods : Male Sprague-Dawley rats received acupuncture at bilateral Yanggu($SI_5$) point for 30seconds immediately before the subcutaneous injection of saline or methamphetamine(0.5mg/kg). The total amount of locomotor activity for 90min were measured just before brain samples were taken for immunohistochemistry. Results : Results showed that acupuncture at the specific point $SI_5$, but not control point(Kunlun, $BL_{60}$) significantly reduced locomotor activity and c-Fos expression in the striatum and nucleus accumbens induced by acute administration of methamphetamine. Conclusions : These results suggest that acupuncture may be effective in suppressing the reinforcing effect of methamphetamine by regulating neuronal activity.

• Journal of Preventive Medicine and Public Health
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• 제24권4호
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• pp.465-472
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• 1991

약물남용자들의 C형간염 감염 상태를 파악하고자 methamphetamine 남용에 의한 중독 증상으로 입원하고 있는 141명을 대상으로 하여 약물남용의 빈도와 기간, 약물 투여경로 등에 관한 조사와 함께 혈청내 C형간염 항체, B형간염 표식자 및 간기능 검사를 실시하고 이들 사이의 관련성을 검토하여 다음과 같은 결과를 얻었다. 1. C형간염 항체 양성률은 60.3%(85/141)이었으며 성별로는 차이가 없었으나 연령군별로는 40세 이상군에서 증가되는 경향이 있었다. 2. C형간염 항체의 양성률은 약물남용의 빈도가 높았던 군과 약물남용의 기간이 길었던 군에서 유의하게 높았으나 B형간염 표면항원의 양성률은 약물남용의 빈도 및 기간과 유의한 차이가 없었다. 이상의 결과로 보아 methamphetamine 남용자에서 C형간염 유병률은 매우 높을 것이며 감염 전파경로에 있어서 C형간염은 반복적인 경주적 폭로가 중요 전파 요인일 것으로 추측된다.

• 대한임상독성학회지
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• 제19권1호
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• pp.24-30
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• 2021

Purpose: In Korea, it is predicted that the proportion of drug abusers among patients visiting the emergency room will soon increase. Several emergency medical institutions in Korea are conducting field urine screening tests for poisoning. In this study, we investigated the characteristics and usefulness of urine toxicology screening tests. Methods: The medical records of patients with positive results for tetrahydrocannabinol and methamphetamine from urine toxicology screening tests at a tertiary university hospital from August 2016 to August 2019 were reviewed retrospectively. The subjects were classified into positive and false-positive groups, and their clinical characteristics were compared and analyzed. Results: Of the 2,026 patients surveyed, 823 patients (40.6%) tested positive for one or more drugs. Among them, 12 cases (0.6%) were positive for methamphetamine and 40 cases (2.0%) were positive for tetrahydrocannabinol. The positive and the false-positive rates for methamphetamine were 66.7% and 33.3%, respectively. The positive and the false-positive rates for tetrahydrocannabinol were 2.5% and 97.5%, respectively. Conclusion: Methamphetamine showed a relatively low false-positive rate in our study. Therefore, this test seemed to assist in diagnosing methamphetamine poisoning when considered together with the present illness and physical examination results. On the other hand, the high false-positive rate for tetrahydrocannabinol tests indicates that this test was unlikely to assist in diagnosing tetrahydrocannabinol poisoning. However, considering the growing trend of illegal drug abusers in Korea, it may still be useful as a diagnostic tool for identifying drug users.

• Bulletin of the Korean Chemical Society
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• 제27권3호
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• pp.407-412
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• 2006

Bioluminescence single-site immunometric assay for methamphetamine (MA) using the native aequorin, a photoprotein, as a signal generator was developed for the first time. MA is a potent sympathomimetic amine with stimulant effects on the central nervous system. MA abuse induces hallucinations and, thus, may cause a serious social problem. The single-site immunometric MA assay was optimized and its dose-response behavior was examined. The dose-response curve shows that the detection limit is 1.1 ${\times}$ $10^{-10}$ M and a dynamic range is four orders of magnitude with 15 $\mu$g/mL BSA-MA conjugate and 1.0 ${\times}$ $10^{-8}$ M anti-MA antibody-biotin conjugate. In order to evaluate this assay, the structurally similar compounds, amphetamine, ephedrine, norephedrine, benzphetamine and N-4-(aminobutyl)methamphetamine were examined for their crossreactivity. None of these five compounds showed any cross-reactivity. Additionally, an artificial urine solution spiked with MA was analyzed by the MA assay, and the result of the analysis demonstrated the usefulness of the present assay for the determination of MA in urine.

• Mass Spectrometry Letters
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• 제10권1호
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• pp.1-10
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• 2019

Amphetamine-type stimulants (ATS) are a group of ${\beta}$-phenethylamine derivatives that produce central nervous system stimulants effects. The representative ATS are methamphetamine and 3, 4-methylenedioxymethamphetamine (MDMA), and abuse of ATS has become a global problem. Methamphetamine is abused in North America and Asia, while amphetamine and 3, 4-methyle nedioxym ethamphetamine (Ecstasy) are abused in Europe and Australia. Methamphetamine is also the most abused drug in Korea. In addition to the conventional ATS, new psychoactive substances (NPS) including phenethylamines and synthetic cathinones, which have similar effects and chemical structure to ATS, continue to spread to the global market since 2009, and more than 739 NPS have been identified. For the analysis of ATS, two tests that have different theoretical principles have to be conducted, and screening tests by immunoassay and confirmatory tests using GC/MS or LC/MS are the global standard methods. As most ATS have a chiral center, enantiomer separation is an important point in forensic analysis, and it can be conducted using chiral derivatization reagents or chiral columns. In order to respond to the growing drug crime, it is necessary to develop a fast and efficient analytical method.

• 대한한의학방제학회지
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• 제19권2호
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• pp.39-46
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• 2011

Objectives : The BangPungTongSungSan(BPTSS) has been used as a therapeutic agent for cerebrovascular disease, cerebral hemorrhage, hypertension, diabetes and obesity in oriental medicine. The present study designed to investigate the effect of BPTSS on behavioral change and neuronal activation induced by acute methamphetamine(METH, 2 mg/kg, i.p.) in C57BL/6 mice. Methods : Mice received the oral administration of BPTTS(25, 50 and 100 mg/kg) 1 h prior to saline or METH administration. Locomotor activity was measured for 90 min using videotractking method and c-Fos expression, as marker of neuronal activation, was identified in a separate groups of mice by immunohistochemistry. Results and conclusions : Methamphetamine injection significantly increased locomotor activity and c-Fos expression in the nucleus accumbens and striatum. Interestingly, BPTTS(100 mg/kg) significantly suppressed locomotor activity and c-Fos expression in the nucleus accumbens and striatum by acute exposure to METH. These results suggest that BangPungTongSungSan may be effective in suppressing the reinforcing effect of methamphetamine by modulation neuronal activity.

• 한국학교ㆍ지역보건교육학회지
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• 제10권2호
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• pp.55-68
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• 2009

연구목적: 본 연구는 미국 인디애나 주에 소재하는 중학교와 고등학교 학생들 사이의 methamphetamine, MDMA, GHB, and Rohypnol과 같은 약물사용 특성을 파악하고 청소년 약물사용과 관련된 성과 학교 환경에 대한 인지된 안전도 특성을 분석하였다. 연구방법: 본 분석에 사용된 데이터는 Indiana Prevention Resource Center (IPRC)에서 2007 년도에 수행한 음주, 흡연, 약물사용 설문조사(ATOD) 응답자료로서 인디애나 주에 거주하고 있는 6-12 학년 사이의 학생들을 편의추출하여 조사가 가능했던 158,632명의 응답을 얻었다. 연구결과: 로지스틱 회귀분석에서 조사한 4개 약물을 사용하는 학생들의 관심도는 교실에 있을 때, 복도에서 혼자 있을 때, 방과후 활동을 위해 학교에 남아있을 때 불안전하다고 느끼는 약물 미사용자보다 더 높은 경향을 보였다. GHB, MDMA, Rohypnol을 사용한다고 응답한 학생이 여자보다 남자에서 더 많았고 상관관계분석 결과, 메탐페타민을 비롯한 네 가지 약물 사용의 상호간 상관도가 모두 유의미하게 높았다.

• Toxicological Research
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• 제6권2호
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• pp.121-130
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• 1990

Pregnant Wister rats were given daily subcutaneous administrations of methamphetamine (MAPT; varying doses ranging from 1.0 to 4.5mg/kg) from days 7 to20 of gestation and teratogenic effects have been determined. The teratogenic effects inducible with orally administered caffeine (90mg/kg/day)for the same durations were used as the positive controls. MAPT doses greater than 2.0 mg/kg have suppressed the rate of maternal weight gain. Some of the offsprings (F1) of the prenatal MAPT treated groups had decreased growth rate and delayed development of physical characters and functional reflexes. The male offsprings of the MAPT treated groups had significant decreases in their spontaneous motor activity but had enhanced conditioned avoidance responses. However, the mating performances of these offsprings were not affected. These results indicated that prenatal exposure of MAPT may induce some behavioral teratogenicity in rats.

• Archives of Pharmacal Research
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• 제16권3호
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• pp.175-179
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• 1993

Gas chromoatography with flame ionization detector (FID) along with mass spectrometry (GC/MS) were used for the screening and quantification of methamphetamine (MA) and its major metabolite, amphetamine (AM0, in blood and urine in eleven fatal cases in which MA abuse was suspected. Postmortem blood MA varied from $0.5-30.2\;\mu{g/ml}$, while Am levels ranged from none detected (6 of 11 cases) to 4.8 .mu.g/ml. Additionally, distribution studies were performed in three of these cases in which tissue smaples were available for evaluation. Liver contained the highest ocncentration of MA among the tissu samples. In eight of the eleven cases, when no other direct cause of death was evident (i.e. 3 cases of traumatic dath0, either no blood AM was found or the ratio of MA/AM was 3.4 or greater. These data are consistent with acute MA use followed by death due to acute drug intoxication or by the occurrence of hypersensitivity and reverse seen in cases of chronic drug abuse.