• 대한수의학회지
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• 제61권3호
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• pp.22.1-22.7
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• 2021

Fenbendazole (FBZ) is a commonly used anthelmintics in veterinary medicine that has recently been found to have anticancer effects in humans. On the other hand, few studies have examined the anti-inflammatory effects of FBZ, and its mechanism is unknown. In this study, mouse bone marrow cells (BMs) were treated with lipopolysaccharide (LPS), a representative inflammation-inducing substance, to generate a situation similar to osteomyelitis in vitro. The effect of FBZ on inflammatory BMs was examined by measuring the metabolic activity, surface marker expression, cell nuclear morphology, and mitochondrial membrane potential (MMP) of BMs. FBZ decreased the metabolic activity and MMP of LPS-treated BMs. Annexin V-fluorescein isothiocyanate/propidium iodide staining and Hoechst 33342 staining showed that FBZ reduced the number of viable cells and induced the cell death of inflammatory BMs. In addition, FBZ reduced the proportion of granulocytes more than B lymphocytes in LPS-treated BMs. Overall, FBZ induces cell death by destabilizing the MMP of LPS-induced inflammatory BMs. In addition to anthelmintic and anticancer agent, FBZ can play a role as an anti-inflammatory agent.

• Applied Biological Chemistry
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• 제13권1호
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• pp.93-96
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• 1970

대두(大豆)의 발아기간(發芽期間) 6일간(日間)에 있어서 자엽(子葉) 및 seedling 양조직(兩組織)의 lactic dehydrogenase 활성(活性)이 어떻게 변화하며 그 변화가 어떤 상관성(相關性)을 갖는가를 살펴 다음과 같은 결과(結果)를 얻었다, 1. 자엽(子葉)의 LDH활성(活性)은 발아후(發芽後) 3일까지의 전반기(前半期)에는 계속 증가(增加)하나 그 후반(後半)의 3일간(日間)은 격감(激減)한다. 2. Seedling의 LDH활성(活性) 역시 발아후(發芽後) 3일(日)의 전반기(前半期)에서는 자엽(子葉) LDH의 증가(增加)와 의의(意義)있는 상관하(相關下)에 증가(增加)하나 자엽(子葉)과는 달라서 후반(換半)에서도 그 상승치(上昇値)를 계속 유지(維持)한다. 3. 따라서 대두(大豆) 자엽(子葉)은 발아후(發芽後) 3일(日)이 되면 대사효율(代謝?率)이 현저(顯著)히 감퇴(減退)하나 seedling에서는 계속 대사효율(代謝?率)이 높이 유지(維持)됨을 알았다.

• Journal of Korean Biological Nursing Science
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• 제13권3호
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• pp.245-252
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• 2011

Purpose: This study was designed to find the correlations between physical activity and insulin resistance of the middle-aged adults. Methods: One hundred thirty one subjects participated in this study were age 40-60 from Y university's center for physical exercise in W city. The data were collected from August 5 to October 5, 2009. To measure physical activity, the contracted Korean version of the Self-Report of Physical Activity Questionnaires of IPAQ was used. Insulin resistance was measured using fasting glucose levels, serum insulin levels, and HOMA method (serum insulin${\times}$fasting glucose/22.5). Results: The continuous physical activity overall in this study was on average $1,792.30{\pm}2,216.81$ MET (min/week), and as a result of categorical classification: no activity was 66 subjects (50.4%); minimum activity, 41 (31.3%); and health-improving activity, 24 (18.3%), respectively. The overall degree of insulin resistance in these subjects was $2.20{\pm}2.62$(0.28-12.74). There was negative correlation between moderate intensity activity and insulin resistance (r= -.189, p<.05). Conclusion: These results revealed that promoting moderate-intensity physical activity is important in preventing and improving insulin resistance and possibly other metabolic risk factors in the middle-aged adults.

• 대한핵의학회지
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• 제33권1호
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• pp.28-39
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• 1999

목적: 내측성 외측성 측두엽 간질에서 F-18-FDG PET으로 각각 측두엽 내외측의 대사가 감소된 정도를 비교하였다. 대상 및 방법: 수술 후 관찰 결과와 병리 소견으로 확진한 내측성 측두엽 환자 19명과 구조병변이 없거나(14명) 있는(11명) 25명의 외측성측두엽 간질환자에서 F-18-FDG PET에 나타난 양쪽 측두엽 내측부와 외측부의 비대칭계수를 측정하여 비교하였다. 결과: 내측성 측두엽 간질 환자는 내측 측두엽과 외측 측두엽의 비대칭계수가 모두 높았다. 외측성 측두엽 간질은 구조병변이 있는 경우 외측 측두엽의 대사 감소가 심하지만 내측은 비대칭계수가 정상(7/11)이거나 감소(4/11)하였다. 구조병변이 없는 경우 내측 외측 모두 전체(14)의 반 수(내측 43%, 뇌측 56%)에서만 대사가 감소하여 비대칭계수가 정상범위를 벗어났다. 내측성 측두엽 간질과 병변 없는 외측성 측두엽 간질은 외측 측두엽의 패사가 감소했다는 점에서 차이를 찾을 수 없었다. 내측 측두엽의 대사가 정상범위일 때 외측성 측두엽간질일 가능성이 컸다. 결론: 준정량적 지표로 비대칭계수를 구하여 얻은 위의 결과가 F-18-FDG PET에서 산출한 내측 측두엽의 비대칭계수로 내측성 측두엽 간질인지 외측성 측두엽 간질인지 감별할 수 있을 것임을 시사하였다.

• 보건교육건강증진학회지
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• 제29권2호
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• pp.93-105
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• 2012

Objectives: The objective of the study was to examine the clustering patterns and correlates of multiple health behaviors (MHBs) in middle-aged Koreans with metabolic syndrome (MetS). Methods: Data on sociodemographics, clinical characteristics, health behaviors (vegetable intake, physical activity, cigarette smoking, and alcohol consumption), and psychological characteristics were collected by a self-reported survey and medical examination from 331 individuals with MetS. Clustering of MHBs was examined by measuring 1) the ratios of observed and expected prevalence of MHBs, and 2) the prevalence odds ratios. A binomial logistic regression were conducted. Results: Men were more likely than women to engage in multiple unhealthy behaviors. Clustering of smoking and heavy drinking was exhibited in the participants. Women with high vegetable intake were more likely to be physically inactive, and those with inadequate vegetable intake were more likely to be physically active. Those with lower self-regulation were more likely to engage in unhealthy behaviors. Conclusions: The findings support the multiple health behavior approach as opposed to the individual health behavior approach. Emphasis of self-regulation is necessary in developing multiple behavior intervention for individuals with MetS.

• 제어로봇시스템학회논문지
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• 제20권5호
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• pp.486-494
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• 2014

Intelligent homes consist of ubiquitous sensors, home networks, and a context-aware computing system. These homes are expected to offer many services such as intelligent air-conditioning, lighting control, health monitoring, and home security. In order to realize these services, many researchers have worked on various research topics including smart sensors with low power consumption, home network protocols, resident and location detection, context-awareness, and scenario and service control. This paper presents the real-time metabolic rate estimation method that is based on measured heart rate for human adaptive appliance (air-conditioner, lighting etc.). This estimation results can provide valuable information to control smart appliances so that they can adjust themselves according to the status of residents. The heart rate based method has been experimentally compared with the location-based method on a test bed.

• Food Science and Biotechnology
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• 제16권3호
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• pp.457-462
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• 2007

Histone acetyltransferase (HAT) is a family of enzymes that regulate histone acetylation. Dysfunction of HAT plays a critical role in the development of cancer. Here we have screened the various plant extracts to find out the potent HAT inhibitors. The bellflower (Platycodon grandiflorum) root have exhibited approximately 30% of the inhibitory effects on HAT activity, especially p300 and CBP (CREB-binding protein) at the concentration of $100\;{\mu}g/mL$. The cell viability was decreased approximately 52% in LNCaP cell for 48 hr incubation. Furthermore, mRNA level of 3 androgen receptor target genes, PSA, NKX3.1, and TSC22 were decreased with bellflower root extract treatment ($100\;{\mu}g/mL$) in the presence of androgen. In ChIP assay, the acetylation of histone H3 and H4 in PSA promoter region was dramatically repressed by bellflower root treatment, but not TR target gene, Dl. Therefore, the potent HAT inhibitory effect of bellflower root led to the decreased transcription of AR target genes and prostate cancer cell growth with the repression of histone hyperacetylation.

• Journal of Microbiology and Biotechnology
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• 제19권3호
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• pp.299-306
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• 2009

Dissolved oxygen (DO) has a significant effect on the molecular weight of hyaluronic acid (HA) during the fermentation of Streptococcus zooepidemicus. Therefore, to further investigate the effect of DO on the yield and molecular weight of HA, this study compared the metabolic flux distribution of S. zooepidemicus under aerobic conditions at various DO levels. The metabolic flux analysis demonstrated that the HA synthesis pathway, considered a dependent network, was little affected by the DO level. In contrast, the fluxes of lactate and acetate were greatly influenced, and more ATP was generated concomitant with acetate at a high DO level. Furthermore, the has gene expression and HA synthase activity were both repressed under anaerobic conditions, yet not obviously affected under aerobic conditions at various DO levels. Therefore, it was concluded that the HA molecular weight would seem to depend on the concomitant effect of the generation of ATP and reactive oxygen species. It is expected that this work will contribute to a better understanding of the effect of the DO level on the mechanism of the elongation of HA chains.

• Journal of Microbiology and Biotechnology
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• 제28권5호
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• pp.784-795
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• 2018

Bacillus amyloliquefaciens Pc3 was isolated from Antarctic seawater with antifungal activity. In order to investigate the metabolic regulation mechanism in the biosynthesis of lipopeptides in B. amyloliquefaciens Pc3, GC/MS-based metabolomics was used when exogenous indole was added. The intracellular metabolite profiles showed decreased asparagine, aspartic acid, glutamine, glutamic acid, threonine, valine, isoleucine, hexadecanoic acid, and octadecanoic acid in the indole-treated groups, which were involved in the biosynthesis of lipopeptides. B. amyloliquefaciens Pc3 exhibited a growth promotion, bacterial total protein increase, and lipopeptide biosynthesis inhibition upon the addition of indole. Besides this, real-time PCR analysis further revealed that the transcription of lipopeptide biosynthesis genes ituD, fenA, and srfA-A were downregulated by indole with 22.4-, 21.98-, and 26.0-fold, respectively. It therefore was speculated that as the metabolic flux of most of the amino acids and fatty acids were transferred to the synthesis of proteins and biomass, lipopeptide biosynthesis was weakened owing to the lack of precursor amino acids and fatty acids.

• The Korean Journal of Physiology
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• 제4권2호
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• pp.69-81
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• 1970

These studies were carried out on 176 persons ranging in age from 20 to 50 years to determine the basal metabolic rates, energy expenditure of various activities, and daily energy expenditure of service personnel in Korea. The measurements of basal metabolic rates were made on 42 subjects by indirect calorimetry using a Douglas' bag and Scholander's gas analyzer. The energy expenditures of various activities of daily life were also measured. The greatest increase in ratio of energy expenditure in the basis of resting metabolism was 277.3% in floor sopping and the least was 40.9% during hair cutting by beauticians. The assessment of the dailly energy expenditure for each subject was made by the factorial method, using a record of their activities throughout each of 24 hours of every survey day. Certain activities were recorded in minute units. The total daily energy expenditure is the sum of all energy expenditure. This was calculated by multiplying the caloric value of the metabolic rate by the time spent on each activity. The result of the total daily energy expenditure records for 17 occupations are summarized. In respect to the daily energy expenditure, most of the occupations are moderate or light work. But the janiter (F), laundress, cook (F), room maid and nurse's aid do heavy work.