• Journal of Microbiology and Biotechnology
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• 제14권5호
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• pp.919-923
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• 2004

The sk10 isolated from kimchi was identified as W. kimchii on the basis of l6s-rDNA sequencing. Studies were made to analyze the metabolic flux shift of the sk10 on glucose under aerobic growth conditions. The sk10 produced 38.2 mM acetate, 16.3 mM ethanol, and 33.2 mM lactate under aerobic conditions, but 2.4 mM acetate, 48.0 mM ethanol, and 44.1 mM lactate under anaerobic conditions. The NADH peroxidase (NADH-dependent hydrogen peroxidase) activity of sk10 grown under aerobic conditions was 11 times higher than that under anaerobic conditions. Under the low ratio of $NADH/NAD^+$, the metabolic flux toward lactate and ethanol was shifted to the flux through acetate kinase without NADH oxidation. The kinds of enzymes and metabolites of sk10 were close to those in the pathway of Leuconostoc sp., but the metabolites produced under aerobic growth conditions were different from those of Leuconostoc sp. The stoichiometric balance calculated using the concentrations of metabolites and substrate was about 97%, coincident with the theoretical values under both aerobic and anaerobic conditions. From these results, it was concluded that the metabolic flux of W. kimchii sk10 was partially shifted from lactate and ethanol to acetate under aerobic conditions only.

• Journal of Microbiology and Biotechnology
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• 제27권6호
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• pp.1157-1162
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• 2017

The goal of this study was to identify and characterize selected Trichoderma isolates by metabolic profiling and enzyme assay for evaluation of their potential as biocontrol agents against plant pathogens. Trichoderma isolates were obtained from the Rural Development Administration Genebank Information Center (Wanju, Republic of Korea). Eleven Trichoderma isolates were re-identified using ribosomal DNA internal transcribed spacer (ITS) regions. ITS sequence results showed new identification of Trichoderma isolates. In addition, metabolic profiling of the ethyl acetate extracts of the liquid cultures of five Trichoderma isolates that showed the best anti-Phytophthora activities was conducted using gas chromatography-mass spectrometry. Metabolic profiling revealed that Trichoderma isolates shared common metabolites with well-known antifungal activities. Enzyme assays indicated strong cell wall-degrading enzyme activities of Trichoderma isolates. Overall, our results indicated that the selected Trichoderma isolates have great potential for use as biocontrol agents against plant pathogens.

• 약학회지
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• 제49권4호
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• pp.284-290
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• 2005

The ginseng root has been used as a tonic remedy, and its antidiabetic activity has been demonstrated as early as 1920s. Although wild ginseng was anecdotally thought to be superior to cultivated ginseng in terms of pharmacological properties, there have been no prior reports on its improvement of metabolic syndrome. In this study, we figured out whether wild ginseng ethanol extract (WGEE) exerted the preventive effects on high fat diet-induced metabolic syndrome as well as treatment effect in ICR mice. In the preventive mode experiment, WGEE at 500 mg/kg significantly inhibited body weight gain $(16\%)$, fasting blood glucose $(37\%)$ and insulin $(37\%)$, triglyceride $(15\%)$, and free fatty acid levels $(32\%)$ when compared to those in high fat diet (HFD) fed control group. WGEE-treated mice at doses of 250 and 500mg/kg improved the insulin resistance index by $55\%\;and\;61\%$ compared to the HFD control group, respectively. In the treatment mode experiment, WGEE also markedly reduced the blood glucose levels (210 mg/dl in control group was lowered to 167 mg/dl).Taken together, WGEE has potential as a preventive and treatment agent for metabolic syndrome and deserves clinical trial in the near future.

• The Korean Journal of Physiology and Pharmacology
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• 제26권3호
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• pp.157-164
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• 2022

Disulfiram (DSF) is an aldehyde dehydrogenase inhibitor. DSF has potent anti-cancer activity for solid and hematological malignancies. Although the effects on cancer cells have been proven, there have been few studies on DSF toxicity in bone marrow cells (BMs). DSF reduces the metabolic activity and the mitochondrial membrane potential of BMs. In subset analyses, we confirmed that DSF does not affect the proportion of BMs. In addition, DSF significantly impaired the metabolic activity and differentiation of BMs treated with granulocyte macrophage-colony stimulating factor, an essential growth and differentiation factor for BMs. To measure DSF toxicity in BMs in vivo, mice were injected with 50 mg/kg, a dose used for anti-cancer effects. DSF did not significantly induce BM toxicity in mice and may be tolerated by antioxidant defense mechanisms. This is the first study on the effects of DSF on BMs in vitro and in vivo. DSF has been widely studied as an anti-cancer drug candidate, and many anti-cancer drugs lead to myelosuppression. In this regard, this study can provide useful information to basic science and clinical researchers.

• Journal of Preventive Medicine and Public Health
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• 제46권2호
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• pp.89-95
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• 2013

Objectives: Little is yet known about the determinants of bone mineral density (BMD) in young adults. Thus, in this study, we aimed to determine the factors that have an impact on BMD in young men. Methods: Questionnaires were sent out to 111 male medical students. Information on age, socio-economic status, medical history, lifestyle, physical activity during adolescence, school club participation, current physical activity, and dietary intake were collected by the survey. Height, weight, percent body fat and muscle mass were estimated by bioelectrical impedance, and BMD was obtained using calcaneal quantitative ultrasound. Using the Poisson regression model, prevalence ratios (PRs) were used to estimate the degree of association between risk factors and osteopenia. Results: The height and current physical activity showed a correlation to the Osteoporosis Index. Among the categorized variables, past physical activity during adolescence (p= 0.002) showed a positive effect on the bone mineral content. In the multivariate model, past physical activity (${\geq}1$ time/wk) had a protective effect on osteopenia (PR, 0.37; 95% confidence interval [CI], 0.18 to 0.75) and present physical activity (1000 metabolic equivalent of task-min/wk) decreased the risk of osteopenia (PR, 0.64; 95% CI, 0.44 to 0.91). Conclusions: Past physical activity during adolescence is as important as physical activity in the present for BMD in young men.

• 한국수산과학회지
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• 제28권5호
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• pp.549-556
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• 1995

일본 뢰호내해 빈산소수괴의 형성에 따른 황화수소의 발생이 꽃게, Portunus trituberulatus의 자원량이 미치는 영향을 간접적으로 파악하기 위하여 이들의 생존, 섭이활동 및 대사률에 미치는 빈산소와 황화수소의 복합적 영향에 관한 실험을 하였다. 빈산소조건에서 꽃게의 생존, 섭이활동 및 대사률은 용존산소가 각각 2.14, 1.41, 및 3.35mg/l에서 유의한 저하가 인정되었다. 또한 복합 조건하에서 꽃게의 생존율은 용존산소 1.86mg/l이하에서 황화수소농도 $(12.35 {\mu}g/l)$, 섭이활동은 용존산소 1.86mg/l이하와 황화수소농도 $(12.35 {\mu}g/l)$, 대사률은 용존산소 2.97mg/l이하와 $(13.12 {\mu}g/l)$의 황화수소농도에서 유의한 저하를 나타내었다. 아울러 용존산소 1.86mg/1이하와 황화수소농도 $(12.35 {\mu}g/l)$의 복합 조건하에서는 빈산소조건하에 비해 그들의 생존과 섭이활동율이 한층더 감소하였다. 꽃게의 서식지인 뢰호내해의 각 해역에서는 하기의 용존산소량과 황화수소가 각각 2.0mg/l이하, $15{\mu}g/l)$이 상이 보고되고 있어 이곳에 서식하는 꽃게의 자윈량의 감소가 예상된다.

• 한국초지조사료학회지
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• 제38권4호
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• pp.343-348
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• 2018

In this study, the effect of daily short-distance walking exercise on milk production and metabolic status of the lactating dairy cows was investigated. The experiment was conducted with 10 lactating dairy cows comprising 2 groups (5 dairy cows/group), which were walking activity and non-walking activity. The walking activity (WA) group lactating dairy cows walked outdoors for 1 km/d, and the non-walking activity (non-WA) lactating dairy cows were maintained within a cowshed from $18^{th}$ April to $30^{th}$ May. The activity volume of the neck was not significantly different between the groups, but the activity volume of the leg was higher in WA group compared with non-WA (p<0.05). The rectal temperature was higher in the WA group ($38.6^{\circ}C$) compared with non-WA ($38.2^{\circ}C$) (p<0.05). No significant differences in dietary NE intake, milk production and milk composition were found between the groups. The plasma cortisol concentration was higher in WA group ($2.14{\mu}g/dl$) compared to non-WA ($0.95{\mu}g/dl$) (p<0.05). However, plasma cortisol level of WA dairy cows was significantly decreased as compared with non-WA cows. On the contrary, the level of plasma melatonin was increased in WA cows than that of non-WA dairy cows. It may be interpreted as a change in physical fitness. From this study, we suggest that walking activity can be improved energy balance. Further investigation is needed to determine whether different combinations of distance, rate or doing in morning or afternoon will stimulate energy balance.

• Ocean and Polar Research
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• 제24권1호
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• pp.47-53
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• 2002

To evaluate the effect of reed population on the distribution and activities of microorganisms, vertical distribution of heterotrophic bacteria, degradation rate of cellulose, extracellular aminopeptidase activity (APA) and metabolic diversity based on GN2 Microlog plate were measured at two salt marsh stations in Hogok-ri, Namyang Bay, west coast of Korea. The number of heterotrophic bacteria at station 1 (reed population inhabited area) showed 2 to 6 times higher than that of station 2 (exposed area) with exception in the surface layer. Cellulose degradation rates in station 1 showed more than 50%. month-I and higher than that of station 2 (10.2 to 38.4%. $month^{-1}$). Yet the APA at two stations did not show difference except surface layer and suggested that APA might not be a significant factor in degrading marsh plant debris. Lipid class compounds, cell wall polymers and L-alanine were widely used by microorganisms. The number and activities of bacterial populations especially concerned in plant debris degradation seemed to be stimulated by the reed communities.

• 대한약침학회지
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• 제25권4호
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• pp.364-368
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• 2022

Objectives: Verbenalin is a compound found in herbs such as Cornus officinalis and Verbena officinalis. This study investigated whether verbenalin is safe by analyzing its mutagenicity. Methods: To examine the mutagenic potential of verbenalin, a bacterial reverse mutation test (Ames test) was conducted with Salmonella typhimurium and Escherichia coli strains. Experiments with and without metabolic activity were performed. Results: The mean colony number was less than double that of the control. Growth inhibition and precipitation of verbenalin were not apparent in all strains at different concentrations regardless of metabolic activity. Conclusion: Verbenalin did not show any signs of mutagenicity in this study. Additional toxicity studies including repeated oral toxicity, reproductive toxicity, and carcinogenicity tests are needed.

• 문화기술의 융합
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• 제4권2호
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• pp.139-147
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• 2018

본 연구의 목적은 대사증후군 노인의 운동유지 여부에 따라 운동유지에 대한 영향요인을 확인하기 위해 시행된 횡단적 조사연구이다. 본 연구의 대상자는 총 136명이며, 자료의 수집은 대사증후군 노인의 운동유지 영향요인을 알아보기 위해 운동유지, 운동기대감, 운동 관련 환경, 운동 사회적지지, 운동자기효능감에 대한 도구로 수집되었다. 수집된 자료는 SPSS Win 20.0 program을 사용하여 requency, X2, t-test, Logistic regression으로 분석하였다. 연구 결과 연령, 운동기대감, 운동자기효능감이 이들의 운동유지 영향요인임이 밝혀졌다. 이러한 결과는 대사증후군 노인의 운동유지를 위해서는 내적 동기에 해당하는 자기효능감을 함양시키고 운동기대감을 증진시키는 것이 중요하다고 할 수 있다. 또한 대사증후군 노인을 위한 건강증진프로그램을 계획할 때 운동에 대한 결과를 알려주고 이에 대한 이점을 부각시켜 스스로 운동을 잘 해낼 수 있도록 계획해야 하는 데에 본 연구결과가 기초자료로 활용할 수 있음이 기대된다.