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http://dx.doi.org/10.3831/KPI.2022.25.4.364

Bacterial Reverse Mutation Test of Verbenalin  

Hye Jeong Shin (Department of Korean Medicine, College of Korean Medicine, Woosuk University)
Yi Gun Lim (Department of Korean Medicine, College of Korean Medicine, Woosuk University)
Ji Su Ha (Department of Acupuncture & Moxibustion Medicine, National Medical Center)
Gabsik Yang (Department of Korean Medicine, College of Korean Medicine, Woosuk University)
Tae Han Yook (Department of Korean Medicine, College of Korean Medicine, Woosuk University)
Publication Information
Journal of Pharmacopuncture / v.25, no.4, 2022 , pp. 364-368 More about this Journal
Abstract
Objectives: Verbenalin is a compound found in herbs such as Cornus officinalis and Verbena officinalis. This study investigated whether verbenalin is safe by analyzing its mutagenicity. Methods: To examine the mutagenic potential of verbenalin, a bacterial reverse mutation test (Ames test) was conducted with Salmonella typhimurium and Escherichia coli strains. Experiments with and without metabolic activity were performed. Results: The mean colony number was less than double that of the control. Growth inhibition and precipitation of verbenalin were not apparent in all strains at different concentrations regardless of metabolic activity. Conclusion: Verbenalin did not show any signs of mutagenicity in this study. Additional toxicity studies including repeated oral toxicity, reproductive toxicity, and carcinogenicity tests are needed.
Keywords
dementia; mutagenicity test; verbenalin;
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1 American Psychiatric Association. Diagnostic and statistical manual of mental disorders: DSM-5. 5th ed. Washington, D.C.: American Psychiatric Association; 2013.
2 World Health Organization. Fact Sheets of Dementia [Internet]. Geneva: WHO; 2021 [cited 2022 Jul 7]. Available from: https://www.who.int/health-topics/dementia#tab=tab_1.
3 Oriental Neuropsychiatry Editorial Committee of Korean Medicine schools. Oriental neuropsychiatry: revised ed. Gyeonggi: Jipmoondang; 2011. 332-41 p.
4 Loew D. [Value of Ginkgo biloba in treatment of Alzheimer dementia]. Wien Med Wochenschr. 2002;152(15-16):418-22. German.   DOI
5 Xu SS, Gao ZX, Weng Z, Du ZM, Xu WA, Yang JS, et al. Efficacy of tablet huperzine-A on memory, cognition, and behavior in Alzheimer's disease. Acta Pharmacol Sin. 1995;16(5):391-5.
6 Park SJ, Jung JM, Lee HE, Lee YW, Kim DH, Kim JM, et al. The memory ameliorating effects of INM-176, an ethanolic extract of Angelica gigas, against scopolamine- or Aβ(1-42)-induced cognitive dysfunction in mice. J Ethnopharmacol. 2012;143(2):611-20.   DOI
7 Choi SH, Park CH, Seo JH, Suh YH. Dehydroevodiamine.HCl protects neuron from cytotoxicity induced by Aβ or oxidative stress: its antioxidant. J Neurochem. 2002;81(s1):101-5.
8 Lee JH, Lee EH, Jung EM, Kim DH, Kim SK, Park MH, et al. Perilla Frutescens extract protects against Scopolamine-induced memory deficits in mice. J Physiol Pathol Korean Med. 2021;35(3):97-103.   DOI
9 Gao YM, Wang XL, Deng YX, Reng JM, Shen XC, Guan ZZ, et al. Effect of Gastrodiae Rhizoma powder on prevention of dementia and oxidation resistance in mice. Chin J Exp Tradit Med Formulae. 2020;24:52-8.
10 Bahramsoltani R, Rostamiasrabadi P, Shahpiri Z, Marques AM, Rahimi R, Farzaei MH. Aloysia citrodora Palau (Lemon verbena): a review of phytochemistry and pharmacology. J Ethnopharmacol. 2018;222:34-51.   DOI
11 Bilia AR, Giomi M, Innocenti M, Gallori S, Vincieri FF. HPLCDAD-ESI-MS analysis of the constituents of aqueous preparations of verbena and lemon verbena and evaluation of the antioxidant activity. J Pharm Biomed Anal. 2008;46(3):463-70.   DOI
12 Schonbichler SA, Bittner LK, Pallua JD, Popp M, Abel G, Bonn GK, et al. Simultaneous quantification of verbenalin and verbascoside in Verbena officinalis by ATR-IR and NIR spectroscopy. J Pharm Biomed Anal. 2013;84:97-102.   DOI
13 Cao L, Miao M, Qiao J, Bai M, Li R. The protective role of verbenalin in rat model of focal cerebral ischemia reperfusion. Saudi J Biol Sci. 2018;25(6):1170-7.   DOI
14 Makino Y, Kondo S, Nishimura Y, Tsukamoto Y, Huang ZL, Urade Y. Hastatoside and verbenalin are sleep-promoting components in Verbena officinalis. Sleep Biol Rhythms. 2009;7(3):211-7.   DOI
15 Ferdousi F, Kondo S, Sasaki K, Uchida Y, Ohkohchi N, Zheng YW, et al. Microarray analysis of verbenalin-treated human amniotic epithelial cells reveals therapeutic potential for Alzheimer's disease. Aging (Albany NY). 2020;12(6):5516-38.   DOI
16 Kumar A, Singh A, Ekavali. A review on Alzheimer's disease pathophysiology and its management: an update. Pharmacol Rep. 2015;67(2):195-203.
17 Diehl MS, Willaby SL, Snyder RD. Comparison of the results of a modified miniscreen and the standard bacterial reverse mutation assays. Environ Mol Mutagen. 2000;36(1):72-7.   DOI
18 Maron DM, Ames BN. Revised methods for the Salmonella mutagenicity test. Mutat Res. 1983;113(3-4):173-215.   DOI
19 Korean Neuropsychiatric Association. Textbook of neuropsychiatry. 3rd ed. Seoul: iMiS Company; 2019. 579-89 p.
20 Lane RF, Dacks PA, Shineman DW, Fillit HM. Diverse therapeutic targets and biomarkers for Alzheimer's disease and related dementias: report on the Alzheimer's Drug Discovery Foundation 2012 International Conference on Alzheimer's Drug Discovery. Alzheimers Res Ther. 2013;5(1):5.
21 Wang R, Yan H, Tang XC. Progress in studies of huperzine A, a natural cholinesterase inhibitor from Chinese herbal medicine. Acta Pharmacol Sin. 2006;27(1):1-26.   DOI
22 Takata K, Kitamura Y, Saeki M, Terada M, Kagitani S, Kitamura R, et al. Galantamine-induced amyloid-{beta} clearance mediated via stimulation of microglial nicotinic acetylcholine receptors. J Biol Chem. 2010;285(51):40180-91.   DOI
23 Sastre J, Millan A, Garcia de la Asuncion J, Pla R, Juan G, Pallardo FV, et al. A Ginkgo biloba extract (EGb 761) prevents mitochondrial aging by protecting against oxidative stress. Free Radic Biol Med. 1998;24(2):298-304.
24 Wang BS, Wang H, Wei ZH, Song YY, Zhang L, Chen HZ. Efficacy and safety of natural acetylcholinesterase inhibitor huperzine A in the treatment of Alzheimer's disease: an updated meta-analysis. J Neural Transm (Vienna). 2009;116(4):457-65.   DOI
25 OECD. Test No. 471: bacterial reverse mutation test. Paris: OECD; 1997.