• Journal of the Korean Chemical Society
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• v.24 no.1
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• pp.25-33
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• 1980

Pyrocatechase as a phenolytic dioxygenase was extracted from the benzoate-induced cells of a soil bacterium, a member of Pseudomonadaceae, and purified partially by DEAE-cellulose ion-exchange chromatography and Sephadex G-75 gel filtration. Final preparation of the enzyme yielding 200 fold purification over the crude extracts showed a specific activity of about 40 ${\mu}moles$ per minute per mg protein based on catechol as the substrate. The enzyme showed a very limited substrate specificity towards catechol for its catalytic activity. Based on the inhibition study with the substrate analogues, it was assumed that ortho dihydroxy groups on the aromatic ring may participate in the enzyme-substrate binding. The $K_m$ value for catechol was obtained as $1.9{\times}10^{-6}M$, and the optimum activity of the enzyme was obtained at the pH range of 7∼10 and $35^{\circ}C$. With SH-group blocking agents the enzyme was inhibited seriously. The activity of enzyme was also inhibited by the addition of some heavy metals, $Ag^+$ and $Cu^{2+}$, but was not affected by EDTA. General property of the enzyme was characterized and the possible nature of the enzyme active center was also discussed.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.13 no.1
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• pp.87-95
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• 2000

The two eigenvalues (elastic critical load and natural frequency of lateral vibration) of sinusoidally tapered bats with simply supported ends were determined by the finite element method. For the convenience of structural engineers who are engaged in the structural design or vibration analysis of tapered beam-columns, eigenvalue coefficients were expressed by simple algebraic equations. The validity of each algebraic equation was confirmed by the value of unity for each correlation coefficient. The influence of axial thrust on the lateral vibration frequency was also investigated. For this purpose, the axial thrust was increased successively and the corresponding frequency was calculated. The approximate linear relationship between the axial thrust and the square of the frequency was confirmed lot each of the tapered members.

• Proceedings of the Korean Biophysical Society Conference
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• 2003.06a
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• pp.55-55
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• 2003

P2X$_3$ receptor, a member of P2 purine receptors, is a ligand-gated ion channel activated by extracellular ATP as an endogenous ligand, and highly localized in peripheral and central sensory neurons. The activation of P2X3 receptor by ATP as the pronociceptive effect has been known to initiate the pain signaling involved in chronic inflammatory nociception and neuropathic pain by nerve injury, implicating the possibility of new drug development to control pains. In this study, we have developed a two electrode voltage clamp (TEVC) assay system to evaluate the inhibitory activity of several newly synthesized PPADS and a novel non-ionic antagonist against ATP activation of human P2X3 receptor. PPADS derivatives include several pyridoxine and pyridoxic acid analogs to study the effects of phosphate and aldehyde functional groups in PPADS. All new PPADS analogs were less potent than PPADS at human P2X$_3$ receptors, however, LDD130, a non-ionic analog showed potent antagonistic property with $IC_{50}$/ of 8.34 pM. In order to uncover the structure activity relationships of LDD130, and design new structural analogs, we synthesized and investigated a few structural variants of LDD130, and the results will be discussed in this presentation.

• Proceedings of the Korea Concrete Institute Conference
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• 2008.04a
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• pp.925-928
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• 2008

Fiber is an important ingredient in strain-hardening cementitious composite (SHCC), which can control fracture of cementitious composite by bridging action. The properties of reinforcing fiber, as tensile strength, aspect ratio and elastic modulus, have great effect on the fracture behavior of SHCC. To apply SHCC to structural member, SHCC must have economical efficiency and workability as well as own excellent tensile performance. For these purposes, four-point bending and direct tensile tests on SHCC with only hybrid synthetic fibers, total fiber volume fraction, $V_f$, is 1.5%, are carried out. The research emphasis is on the mechanical properties of SHCC made in Polyvinyl alcohol (PVA) and Polyethylene (PE) fibers, and how this affects the composite property, and ultimately its strain-hardening performance. Also, effect of hybrid type and water-cement ratio on the behavior of SHCC was evaluated in this paper.

• Bulletin of the Korean Chemical Society
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• v.24 no.10
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• pp.1478-1484
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• 2003

CRAMP was identified from a cDNA clone derived from a mouse femoral marrow cells as a member of cathelicidin-derived antimicrobial peptide. Tertiary structure of CRAMP in TFE/$H_2O$ (1 : 1, v/v) solution has been determined by NMR spectroscopy previously and consists of two amphipathic $\alpha-helices$ from Leu4 to Lys10 and from Gly16 to Leu33. These two helices are connected by a flexible region from Gly11 to Gly16. Analysis of series of fragments composed of various portion of CRAMP revealed that an 18-residue fragment with the sequence from Gly16 to Leu33 (CRAMP-18) was found to retain antibacterial activity without cytotoxicity. The effects of two Phe residues at positions 14 and 15 of CRAMP-18 on structure, antibacterial activity, and interaction with lipid membranes were investigated by $Phe^{14,15}$ ${\rightarrow}$ Ala substitution (CRAMP-18-A) in the present study. Substitution of Phe with Ala in CRAMP-18 caused a significant reduction on antibacterial and membrane-disrupting activities. Tertiary structures of CRAMP-18 in 50% TFE/$H_2O$ (1 : 1, v : v) solution shows amphipathic ${\alpha}$-helix, from $Glu^2{\;}to{\;}Leu^{18}$, while CRAMP-18-A has relatively short amphipathic ${\alpha}$-helix from $Leu^4{\;}to{\;}Ala^{15}$. These results suggest that the hydrophobic property of $Phe^{14}{\;}and{\;}Phe^15$ in CRAMP-18 is essential for its antibacterial activity, ${\alpha}$-helical structure, and interactions with phospholipid membranes.

• Journal of Embryo Transfer
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• v.24 no.4
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• pp.259-263
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• 2009

Salivary lipocalin (SAL1) is a member of the lipocalin protein family that has a property to associate with many lipophilic molecules and was identified as pheromone-binding protein in pigs. Our previous study has shown that SAL1 is expressed in the uterine endometrium in a cell type- and implantation stage-specific manner and secreted into the uterine lumen in pigs. However, function of SAL1 in the uterus during pregnancy in pigs is still not known. To understand physiological function of SAL1 in the uterine endometrium during pregnancy in pigs, it needs to elucidate the ligand(s) for SAL1. Thus, to identify the ligand for SAL1 in the porcine uterus, we collected uterine luminal fluid from pigs on day 12 of pregnancy by flushing with PBS. Proteins from the uterine luminal fluid were separated by ion exchange chromatography and gel filtration. Fractions containing SAL1 protein were pooled and concentrated. Immunoblot analysis confirmed successful purification of SAL1. Then, we extracted lipids from the purified SAL1 protein and analyzed the lipids by liquid chromatography-mass spectrometry, and predicted to be steroid hormones and prostaglandins as SAL1 ligands. Results in this study showed that SAL1 protein in the uterine secretions has a small lipophilic molecule as a natural ligand. Further characterization of ligand extracted from purified SAL1 will be useful for understanding physiological function of SAL1 during pregnancy and its application to increase the pregnancy rate in pigs.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.39 no.3
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• pp.369-380
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• 2019

When a extreme loading such as blast is applied to prestressed concrete (PSC) structures and infrastructures for an instantaneous time, serious property damages and human casualties occur. However, a existing design procedure for PSC structures such as prestressed containment vessel (PCCV) and gas storage tank do not consider a protective design for extreme internal blast scenario. Particularly, an internal blast is much more dangerous than that of external blast. Therefore, verification of the internal blast loading is required. In this paper, the internal blast resistance capacity of PSC member is evaluated by performing internal blast tests on RC and bi-directional PSC scaled down specimens. The applied internal blast loads were 22.68, 27.22, and 31.75 kg (50, 60, and 70 lbs) ANFO explosive charge at 1,000 mm standoff distance. The data acquisitions include blast pressure, deflection, strain, crack patterns, and prestressing force. The test results showed that it is possible to predict the damage area to the structure when internal blast loading occurs in PCCV structures.

• Journal of the Korea Institute of Information Security & Cryptology
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• v.12 no.6
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• pp.81-92
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• 2002

In some practical circumstances, the ability of a signer should be restricted. In group signature schemes, a group member of a group may be allowed to generate signatures up to a certain number of times according to his/her position in the group. In proxy signature schemes, an original signer may want to allow a proxy signer to generate a certain number of signatures on behalf of the original signer. In the paper, we present signature schemes, called c-times signature schemes, that restrict the signing ability of a signer up to c times for pre-defined value c at set-up. The notion of c-times signature schemes are formally defined, and generic transformation from a signature scheme to a c-times signature scheme is suggested. The proposed scheme has a self-enforcement property such that if a signer generates c+1 or more signatures, his/her signature is forged. As a specific example, we present a secure c-times signature scheme $^c$DSA based on the DSA (Digital Signature Algorithm) by using a threshold scheme. Our transformation can be applied to other ElGamal-like signature schemes as well.

• Journal of Arbitration Studies
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• v.32 no.2
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• pp.85-114
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• 2022

When human rights disputes are related to the cross-border investments treaties, the investment arbitral tribunals are confronted with the question of how to adjudicate connected human rights violations. The traditional structure restricts arbitration proceedings to the parties named within an investment treaty, i.e., Investor-Claimant and State-Respondent. If human rights issues occur, States must act as proxies for citizens with human rights claims. This effectively excludes individuals or groups with human rights concerns and contradicts the premise of international human rights law that seeks to empower human rights-holders to pursue claims directly and on an international stage. The methods for intorducing human rights issues in the context of investment arbitration proceedings are suggested as follows: First, human rights arguments can be introduced into ISDS by the usual initiator of investment disputes: the investor as the complainant. Especially, if the jurisdictional and applicable law clauses of the respective international investment agreements are sufficiently broad to include human rights violations, adjudicating a pure human rights claim could be possible. Second, the host state may rely on human rights argumentation as a respondent of an investor claim. Human rights have played a role as a justification for state measures undertaken to comply with human rights laws. Third, third party interventions by NGOs and civil society groups as amici curiae may act as advocates for affected populations or communities in response to the reluctance of governments to introduce their own human rights duties into the investment dispute. Finally, arbitrators have also referred to human rights ex officio, i.e., without having a dispute party referring to the specific argument. This was mainly the case in the context of determining the scope of property rights and the existence of an expropriation. As all U.N. member states have human rights obligations, international investment laws must be presumed to be in conformity with the relevant human rights obligations.

• Conservation Science in Museum
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• v.27
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• pp.57-66
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• 2022

This study conducted conservation treatment of a briefcase used by a soldier who fought in the Korean War as a member of the United Nations Forces. The zooid characteristics of its main material, leather, were analyzed in collaboration with the researchers at the National Palace Museum of Korea who have conducted relevant research. A plan for conservation treatment was established based on the results of this analysis. The briefcase was made of leather with a wooden frame. The many densely arranged tiny pores in different patterns on the leather's surface suggests calf leather. The wooden frame and leather had suffered deformation over long years of deterioration and thus required conservation treatment to enhance their structural stability. The conservation treatment began with the removal of contaminants from the exterior and interior of the briefcase. The stiffened leather was softened through humidification, and torn or separated areas were restored using cowhide glue. It is expected that the results of this conservation treatment and analysis of the leather material will serve as useful sources for basic research on leather items as well as wartime and military items.