• Journal of the Korean Society of Industry Convergence
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• v.26 no.5
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• pp.845-853
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• 2023

The Ministry of Employment and Labor announced a roadmap for reducing serious accidents to create a "safe and healthy workplace, happy Korea" and ordered a risk assessment for all work processes in all workplaces on November 30, 2022. Accordingly, even workplaces that handle new chemicals must conduct risk assessment when introducing new chemicals, but the risk assessment techniques currently proposed by the government focus on preventing conventional accidents such as falls, getting caught, and bumping. Currently, there is a Chemical Hazard Risk Management (CHARM) technique that is most commonly used for chemical risk assessment, but in small and medium-sized businesses that lack professional manpower related to chemical substances, there are many technical terms in the technique and it is difficult to interpret, so its utilization is very low. Therefore, the purpose of this study is to present a method that can make risk assessment easier and simpler before handling new chemicals so that it can be used well in small and medium-sized businesses that lack professional manpower related to chemical substances. Through actual cases, the method of using CHARM was confirmed step by step, and based on this, risk reduction establishment and execution methods were derived. Based on the results of this study, it is expected that it will be used as the best tool for preventing accidents such as acute poisoning when handling chemicals in small and medium-sized workplaces that lack professional manpower related to chemical substances and make every effort to protect workers' health.

• Korean Journal of Medicinal Crop Science
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• v.2 no.3
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• pp.211-218
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• 1994

This experiment was conducted to obtain basic information for the establishment of in vitro initial culture system in Fritillaria thuubergii Miq. Methods of surface sterilization of scale segments as explant and effect of antibiotics added into the culture medium on contamination of explant and chilling treatment of mother bulb on bulblet formation were investigated. Portent of contamination of cultured scale segments was significantly higher in the outer scale segments which were unsuitable as initial culture explant than inner scale segments. Contamination of explants taken from inner scale of bulb was reduced by surface sterilizing explants in the solution of $4{\sim}5%$ sodium hypoclorite for $10{\sim}15$ mimutes. Addition of antibiotics such as kanamycin, vancomycia cefotaxim, agrirnycin and agreptomycin and dithane as fungicide and$lncyte^{tm}$ into MS medium was effective to reduce bateriological contamination, but did not work to control fungi. It had effective to delay the degree of contamination caused by fungi and bacteria haboring in cultured explants. Bulblet formation from cultured scale segments was promoted by dry storage for $2{\sim}4$ weeks or moisture storage of mother bulbs for $4{\sim}6$ weeks at $10^{\circ}C$ before excision of explants. Addition of kinetin into medium could not exerted for the bulblet formation from the scale segment of dry storaged bulb compared to control. But explant taken from 6 week moisture storaged bulb formed more than 10 bulblets per explant on the medium containing $3{\sim}5mg/L$ kinetin.

• Environmental Analysis Health and Toxicology
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• v.30 no.sup
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• pp.1.1-1.5
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• 2015

Objectives Despite the great contribution made by chemical substances to the development of modern civilization, their indiscriminate use has caused various kinds of damage to the global environment and human beings. Accordingly, the major developed countries and international society have tried to ensure the safe use of chemicals and a reduction in the use of hazardous chemicals through the establishment of the United Nations Environment Programme and various international agreements. In this reason, we tried to introduce about Green Chemistry progress at the present in worldwide and Korea. Methods We checked and analyzed relative journals, reports using keyword as like Green Chemistry, alternative chemicals, eco-friendly etc. and major country's government homepage search. Results Green Chemistry theory, which argues for the reduction or removal of harmfulness in chemicals throughout their entire life-cycle, has been spreading, and major developed countries, such as the US and Denmark, have developed and operate programs to provide reliable chemical information to help replace hazardous chemicals. Korea has also been conducting studies as like eco-innovation project. Through this project the "Alternative Chemical Search program," has been developed, distributed, and operated since 2011 to provide reliable information to small and medium-sized businesses that have difficulties collecting information to ensure conformity to international regulations. The program provides information that includes the regulations of major countries and Korea, information on 340 alternative chemicals, 70 application cases, and 1:1 consulting. Conclusions The Alternative Chemical Search program is expected to contribute to the establishment of response systems for regulation of Korean small and medium-sized businesses, and it also will be used to provide basic data for Korean hazardous chemical regulation, together with the Act on the Registration and Evaluation, etc. of Chemical Substances and the Chemical Control act, making it possible to establish an infrastructure for Green Chemistry in Korea and to increase national competitiveness.

• Proceedings of the Korean Society of Environment and Ecology Conference
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• 2009.04a
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• pp.7-20
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• 2009

On the basis of introducing the development process of Chinese nature reserve since first nature reserve was set up in 1959 in China, and comparing the definition of nature reserve between IUCN and China, combining with the objectives and significance of the establishment of Chinese Nature Reserve, the present state and achievements of nature reserve in China were introduced detailedly. And the effect of the laws and rules on the protection in nature and biodiversity was assessed after analyzing the characteristics of the interrelated laws and rules enacted in course of development of Chinese nature reserve over 50 years. Also the qualifications and procedures of nature reserve establishment and relevance aspects of content in China were narrated, and the classification system and management system of Chinese nature reserve and their characteristics were introduced. Finally, the Medium and Long Term Program for Nature Reserve Development in China was introduced, and present some proposals to solve the problems on the basics that discuss the distribution status and its characteristics and problems on managing nature reserve in China.

• Journal of the East Asian Society of Dietary Life
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• v.3 no.2
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• pp.73-81
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• 1993

The purpose of this study is to examine the level of customers' cognition, the patterns of shopping and the level of satisfaction for foods sold in "24-hour market stor", and thus to provide a guideling for establishment of 24-hour market store", and thus to provide a guideline for establishment of 24-hour market store, development of Korean-style food, and enhancement of desirable habit of eating. The study conducted a survey for the young customers (13-19 years old) and the old customers (20-38 years old), the residents in Tague City, for the period Sept. 1-Sept.30. The survey administered to a total of 700 respondents, and finally received 646 ansers. Data analysis incorporates Chi-square test, univariate analysis, Scheffe's test, and correlation analysis, running SPSS Pc+(Statistical Package for Social Science). The results were drawn as follows: In terms of the level of cognition, results show a high level in the aspects of availability, convenience, time-saving in cooking, a variety of goods, easy custody: a medium level in the aspects of taste and sanitation: a low level in the aspects of cheapness and import of foreign food, In terms of customers' shopping patterns, the results show that the respondents answered the most convenience in 24-hour open and nearby location. Shopping was mostly done during the evening time between 18:00 and 22:00, and frequently during the mid-night time. Most of the residents needed a high level in the price. in terms of the level of customers' satisfaction, the results show a high level in the aspects of convenience, packing, and variety ; a medium level in the aspects of taste and sanitation ; a low level in the aspects of nutrition, price, import of foreign goods.

• Korean Journal of Medicinal Crop Science
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• v.20 no.6
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• pp.461-465
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• 2012

This study was carried out to select the appropriate medium (especially, carbon and nitrogen source, potassium phosphate and pH) for somatic embryogenesis in order to develop the rapid mass production system in suspension culture of Oplopanax elatus Nakai. Direct somatic embryos were obtained from root explants in the hormone free suspension culture (MS). Combination of $NH_4NO_3$ and $KNO_3$ at the ratio of 1650 (mg/${\ell}$) : 1900 (mg/${\ell}$) obtained the better result to produce somatic embryo in suspension culture. MS medium supplemented with $170mg/{\ell}$ $KH_2PO_4$. The addition of 1 and 3% sucrose was effective for formation of embryogenic callus. Therefore, this report will be helped to improve the establishment for suspension culture in Oplopanax elatus Nakai.

• Fisheries and Aquatic Sciences
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• v.16 no.3
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• pp.177-185
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• 2013

The development of species-specific fish cell lines has become a valuable tool for biological research. In recent years, marine medaka Oryzias dancena has been recognized as a good experimental model fish but there are no reports of establishment of cell lines from this fish. In this study, two cell lines from O. dancena blastula embryos were established from 41 total trials (4.9%). The two cell lines displayed typical in vitro morphology and have been cultured for >121 passages, which corresponds to 293 days. The doubling times of the cell lines were 29.84 and 28.59 h, respectively, and both possessed the potential to expand in a clonal manner, albeit with significant differences between the two cell lines. The absence of any of the four main medium supplements; i.e., fish serum, fetal bovine serum, basic fibroblast growth factor, and medaka embryo extract, significantly inhibited growth. The proportion of cells possessing normal chromosome number was 45% and 46.7% of the cell lines, respectively. Taken together, two cell lines that proliferate continuously were established from marine medaka and these cell lines may provide a basic tool for characterizing the unique features of this fish species.

• East Asian Journal of Business Economics (EAJBE)
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• v.10 no.3
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• pp.51-61
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• 2022

Purpose - The purpose of this study is to establish the government's logistics policy by calculating the logistics cost of the company and grasping the management status, to reduce the logistics cost of the related companies and to provide basic statistical data necessary for the management strategy. This work examines some associations between reasons for reducing corporate logistics costs (RCLC) and corporate classification such as industry and sales size. Research design, data, and methodology - The survey was conducted in 2018 for 2,000 companies based on the business of mining, manufacturing and wholesale and retail industries since 2010. The survey population is 94,976, of which 92,708 are small and medium enterprises and 2,268 are large corporations. The association among factors may be statistically and visually explored by using chi-squared test and correspondence analysis. Result - This study reveals the association between reasons for RCLC and corporate classification and properties and closeness that exist between the categories of each factor can be mined. Conclusion - As a task to reduce logistics costs of industrial products, expansion and operation of joint logistics business, establishment of cooperative logistics network, and establishment of ordinance on support for smart distribution logistics can be proposed.

• Journal of Microbiology and Biotechnology
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• v.10 no.3
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• pp.367-374
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• 2000

The composition of dissolved gases and nutrients in a liquid medium were determined for establishment of the optimum conditions for in vitro culture of Helicobacter pylori. A microaerobic condition facored by the organism was prepared by adjusting the partial pressure of the gas, agitation speed, and viscosity of the medium. The gaseous concentrations were controlled by utilizing CampyPak Plus that reduced oxygen while augmenting carbon dioxide. Agitation of the broth facilitated the oxygen transfer to the cells, yet inhibited the growth at high rates. An increase of viscosity in the medium repressed the culture although this variable was relatively insignificant. The chemical constituents of the liquid broth were examined to establish an economic model for H. pylori cultivation. The microbe required a neutral pH for optimum growth, and yet was also able to proliferate in an acidic condition, presumably by releasing the acidity-modulating enzyme, urease. Cyclodextrin and casamino acid were investigated as growth enhancers in place of serum, while yeast extract unexpectedly inhibited the cells. A low concentration of glucose, the unique carbon source for the organism, increased the cell density, yet high concentrations resulted in an adverse effect. Under optimally dissolved gas conditions, the cell concentration in brucella broth supplemented with serum substitutes and glucose reached $1.6{\times}10^8$ viable cells/ml which was approximately 50% higher than that obtained in the liquid medium added with only cyclodextrin or serum.

• Journal of Microbiology and Biotechnology
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• v.23 no.8
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• pp.1176-1184
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• 2013

Anti-hepatocyte growth factor (anti-HGF) monoclonal antibodies (mAbs) are potential therapeutics against various cancers. Screening for high-producer clones is a time-consuming and complex process and is a major hurdle in the development of therapeutic mAbs. Here, we describe an efficient approach that allows the selection of high-producer Chinese hamster ovary (CHO) cell lines producing the novel anti-HGF mAb SFN68, which was generated previously by immunizing HGF bound to its receptor c-Met. We selected an SFN68-producing parental cell line via transfection of the dihydrofolate reductase-deficient CHO cell line DG44, which was preadapted to serum-free suspension culture, with an SFN68-expression vector. Subsequent gene amplification via multiple passages of the parental cell line in a methotrexate-containing medium over 4 weeks, followed by clonal isolation, enabled us to isolate two cell lines, 2F7 and 2H4, with 3-fold higher specific productivity. We also screened 72 different media formulated with diverse feed and basal media to develop a suboptimized medium. In the established suboptimized medium, the highest anti-HGF mAb yields of the 2F7 and 2H4 clones were 842 and 861 mg/l, respectively, which were about 10.5-fold higher than that of the parental cell line in a non-optimized basal medium. The selected CHO cell lines secreting high titers of SFN68 would be useful for the production of sufficient amounts of antibodies for efficacy evaluation in preclinical and early clinical studies.