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http://dx.doi.org/10.7783/KJMCS.2012.20.6.461

Establishment of Suspension Culture System to Induce Somatic Embryo in Oplopanax elatus Nakai  

Kim, Hee Young (Department of Applied Plant Sciences, College of Agriculture and Life Science, Kangwon National University)
Seong, Eun Soo (Bioherb Research Institute, Kangwon National University)
Lee, Jae Geun (Department of Applied Plant Sciences, College of Agriculture and Life Science, Kangwon National University)
Yoo, Ji Hye (Department of Applied Plant Sciences, College of Agriculture and Life Science, Kangwon National University)
Hwang, In Seong (Department of Applied Plant Sciences, College of Agriculture and Life Science, Kangwon National University)
Kim, Myong Jo (Department of Applied Plant Sciences, College of Agriculture and Life Science, Kangwon National University)
Lim, Jung Dae (Department of Herbal Medicine Resource, Kangwon National University)
Kim, Na Young (Food Service Cuisine Songho College)
Yu, Chang Yeon (Department of Applied Plant Sciences, College of Agriculture and Life Science, Kangwon National University)
Publication Information
Korean Journal of Medicinal Crop Science / v.20, no.6, 2012 , pp. 461-465 More about this Journal
Abstract
This study was carried out to select the appropriate medium (especially, carbon and nitrogen source, potassium phosphate and pH) for somatic embryogenesis in order to develop the rapid mass production system in suspension culture of Oplopanax elatus Nakai. Direct somatic embryos were obtained from root explants in the hormone free suspension culture (MS). Combination of $NH_4NO_3$ and $KNO_3$ at the ratio of 1650 (mg/${\ell}$) : 1900 (mg/${\ell}$) obtained the better result to produce somatic embryo in suspension culture. MS medium supplemented with $170mg/{\ell}$ $KH_2PO_4$. The addition of 1 and 3% sucrose was effective for formation of embryogenic callus. Therefore, this report will be helped to improve the establishment for suspension culture in Oplopanax elatus Nakai.
Keywords
Oplopanax elatus Nakai; Somatic Embryogenesis; Suspension Culture;
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Times Cited By KSCI : 4  (Citation Analysis)
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