• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2021년도 춘계학술대회
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• pp.55-55
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• 2021

Hibiscus hamabo is a deciduous shrub that grows around salt marshes and is considered a semi-mangrove plant found in Asia. There are no studies on the biological activity of H. hamabo except for studies on the anthocyanin content. We investigated the anti-inflammatory effects of H. hamabo extract (HHE) on lipopolysaccharide (LPS)-induced RAW 264.7 cells. As nuclear factor-kappa B (NF-kB) induced by LPS moves into the nucleus, inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2), and inflammatory cytokines are promoted and the inflammatory reaction begins. The nitric oxide (NO) production decreased by the treatment of HHE. Moreover, mRNA levels of inflammation-related cytokines, such as tumor necrosis factor-α (TNF-α), interleukin (IL)-6, and IL-1β, were significantly suppressed by HHE. Similarly, the expressions of iNOS and COX-2 were also decreased. The phosphorylation of p65, a subunit of NF-κB, was suppressed by HHE. As a result, HHE can be used as an effective natural material for the anti-inflammatory agent.

• 생약학회지
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• 제26권1호
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• pp.62-65
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• 1995

Dopamine ${\beta}-hydroxylase$ (DBH) catalyses the enzymatic reaction of dopamine to norepinephrine. For the purpose of isolating DBH inhibitors from natural resources, thirty one kinds of medicinal plants were screened by tracing the inhibitory activities against bovine adrenal DBH, utilizing tyramine as a substrate. Among the crude drugs tested, leaves of Lactuca sativa L., Gardeniae Fructus, Magnoliae Flos and Scutellariae Radix showed potent enzyme inhibitory activities against DBH.

• Mycobiology
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• 제30권2호
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• pp.88-92
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• 2002

Rehmannia glutinosa is a perennial medicinal plant belonging to the family Scrophulariaceae with more than 300 species known in the world, especially in temperate regions. Its roots have been used widely in Korea for medicinal purposes. However, it is commonly infected by various pathogens during storage, causing great damage to the roots, and impedes the intensive farming of the crop. Therefore, an attempt has been made to isolate and screen a resistance gene against the pathogen Fusarium oxysporum using differential display. We treated salicylic acid(SA), and isolated a resistance gene that responds to SA. As a result, we found that SA was involved in plant defense mechanism in pathogenicity tests with SA treated and non-treted plants, and we isolated a partial PR-la gene through differential display polymerase chain reaction(DD-PCR) method.

• 한국약용작물학회지
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• 제18권6호
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• pp.398-404
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• 2010

Complementary and alternative medicines are considered as a promising research field to develop new therapies for various allergic diseases. In this study, we investigated the anti-allergic effect of Agrimonia pilosa Ledeb (AP) by using passive cutaneous anaphylaxis in mice and its mechanism of action in mast cells. The extract of AP reversibly inhibited degranulation in RBL-2H3 cells and bone marrow-derived mast cells (BMMCs). AP also suppressed the passive cutaneous anaphylaxis inducing by IgE and antigen (Ag) in a dose-dependent manner. In the study to find its mechanism of action, AP inhibited the phosphorylation of Syk kinase, a pivotal protein which is regulated by Src-family kinase for activation of mast cells. In addition, AP also suppressed activation of Akt and Erk1/2 that are critical for the production of cytokines in mast cells. The results strongly suggest that AP exerts anti-allergic activity in vitro and in vivo through the inhibition of activation of Syk in mast cells.

• 대한본초학회지
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• 제27권5호
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• pp.37-44
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• 2012

Objectives : The present study was undertaken to determine whether the ethanol extact of Citri Pericarpium (CP, Pericarp of Citrus unshiu Markovich, Rutaceae) is effective against atopic dermatitis according to it's storage period. Methods : To evaluate antiallergic effect of CP and OCP (Old Citri Pericarpium) evaluated in vivo their inhibitory effects against passive cutaenous anaphylaxis (PCA) reaction induced by IgE-antigen complex and scratching behaviors by compound 48/80. The Anti-atopic effects were measured by contact dermatitis, prurient animal model and PCA reaction. Contact dermatitis in mice as a model of the Type IV reaction caused by Oxazolone. Results : The results showed that anti-pruritus effects, analgesic effects of CP was depends on its hesperetin contents. And It also showed that keep longer in storage appeared to be higher in hesperetin contents. Both CP and OCP(Old CP) have a dose-dependent analgesic action in acetic acid induced writhing syndrome. OCP Potently inhibited PCA reaction in mice, although OCP weakly inhibited in long term contact dermatitis model in mice. Conclusions : These results suggest that the Proportional to the storage period, Citri Pericarpium possesses analgesic effects and anti-allergic effects.

• Biotechnology and Bioprocess Engineering:BBE
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• 제9권1호
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• pp.17-22
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• 2004

A bacterial strain WJ-98 found to produce active extracellular keratinase was isolated from the soil of a poultry factory. It was identified as Paracoccus sp. based on its 16S rRNA sequence analysis, morphological and physiological characteristics. The optimal culture conditions for the production of keratinase by Paracoccus sp. WJ-98 were investigated. The optimal medium composition for keratinase production was determined to be 1.0% keratin, 0.05% urea and NaCl, 0.03% K$_2$HPO$_4$, 0.04% KH$_2$PO$_4$, and 0.01% MgCl$_2$$.$6H$_2$O. Optimal initial pH and temperature for the production of keratinase were 7.5 and 37$^{\circ}C$, respectively. The maximum keratinase production of 90 U/mL was reached after 84 h of cultivation under the optimal culturing conditions. The keratinase from Paracoccus sp. WJ-98 was partially purified from a culture broth by using ammonium sulfate precipitation, ion-exchange chromatography on DEAE-cellulose, followed by gel filtration chromatography on Sephadex G-75. Optimum pH and temperature for the enzyme reaction were pH 6.8 and 50$^{\circ}C$, respectively and the enzymes were stable in the pH range from 6.0 to 8.0 and below 50$^{\circ}C$. The enzyme activity was significantly inhibited by EDTA, Zn$\^$2+/ and Hg$\^$2+/. Inquiry into the characteristics of keratinase production from these bacteria may yield useful agricultural feed processing applications.

• 한국식품저장유통학회지
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• 제19권5호
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• pp.744-750
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• 2012

안전한 천연물을 이용한 천연항산화제 개발 연구에 대한 관심이 고조되는 가운데, 지유, 진피, 천련자, 천문동, 청피, 편축, 하고초 7종의 천연 약용식물 추출물을 통해 항산화 활성을 측정하였다. 추출물의 총 페놀 함량은 지유가 열수추출물과 40% ethanol 추출물에서 각각 31.5 mg/g, 32.7 mg/g으로 가장 높게 나타났다. 추출물의 수용성 물질에 대한 항산화 효과는 DPPH radical 소거능이 지유, 진피, 천련자, 천문동, 청피, 편축, 하고초 7종의 천연 약용식물의 열수 추출물과 ethanol 추출물에서 80% 이상의 높은 소거활성을 나타내었다. ABTS radical cation decolorization을 측정한 결과, 7종의 천연 약용식물의 열수 추출물과 ethanol 추출물에서 85% 이상의 높은 저해율을 나타내어 매우 우수한 항산화 효과를 가지고 있음을 입증하였다. 지용성 물질에 대한 항산화 효과를 측정하기 위해 Antioxidant Protection Factor(PF)를 측정한 결과, 천련자 열수 추출물에서 1.65 PF로 대조구인 vitamin C 보다 높은 수준을 나타내어 지용성 물질에 대한 항산화 활성이 높음을 확인하였다. TBARs 생성 저해율을 측정한 결과 지유, 천련자, 청피, 편축, 하고초의 열수 추출물과 ethanol 추출물에서 99%에 가까운 높은 저해율을 나타내어 대조구인 vitamin C보다 지용성 물질에 대한 항산화 활성이 매우 높은 것으로 확인되었다.

• Journal of Plant Biotechnology
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• 제49권3호
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• pp.240-249
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• 2022

본 연구는 인삼 습해 피해 설정을 확립하고 유전자원을 대상으로 엽록소형광 기법을 이용하여 습해 스트레스 수준을 평가하기 위해 수행되었다. 습해 스트레스 평가는 2년생 묘삼 57개 유전자원 대상으로 25일간 지제부까지 담수처리를 하였다. 피해 정도에 따라 5개 등급으로 분류하여 저항성 유전자원 3개, 감수성 유전자원 3개를 선발하였다. 인삼 유전자원 57개의 지하부 생체중 및 엽록소 함량은 대부분 감소하였고 저항성 유전자원보다 감수성 유전자원의 감소율이 크게 나타났다. 5개의 엽록소형광 매개변수는 습해 스트레스에 노출되는 기간이 길어질수록 증감하는 경향을 보였으며, 특히, Fv/Fm값이 0.8보다 낮으면 광계II의 반응중심이 손상을 입은 상태로 감수성 유전자원의 경우 습해 스트레스에 노출될수록 감소하였다. 본 연구결과에서 유전자원 57개의 최대양자수율(Fv/Fm)은 습해 스트레스 하에서 차이를 보였으며, 습해 스트레스에 저항성을 가진 습해 저항성 유전자원과 감수성 유전자원 간의 차이를 확인할 수 있는 매개변수로 판단되어 인삼 유전자원에 대해 습해 스트레스 수준을 평가할 수 있는 도구로 활용 가능하다고 사료된다.

• 한국약용작물학회:학술대회논문집
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• 한국약용작물학회 2008년도 추계학술발표회
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• pp.487-488
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• 2008

• Toxicological Research
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• 제14권4호
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• pp.489-494
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• 1998

In the present study, we have evaluated cytotoxic effects of Taraxaci Herba extract on human skin melanoma cells. The light microscopic study showed morphological changes AG-NOR (argyrophylic nucleolar organizer region) by silver chloride stain, and glycoprotein by PAS reaction of the treated cells. Disruptions in cell organelles were determined by SRB assay.