• Title/Summary/Keyword: medicinal fungi

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The Genus Chlorociboria, Blue-Green Micromycetes in South Korea

  • Liu, Dong;Wang, Huan;Park, Jung Shin;Hur, Jae-Seoun
    • Mycobiology
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    • v.45 no.2
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    • pp.57-63
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    • 2017
  • The species of the genus Chlorociboria Seaver are very common on the forest floor, and can be easily distinguished by small and numerous blue-green fruitbody, especially the blue substrate dyed with xylindein produced by this group. This genus has rather high species diversity in the Southern Hemisphere, while a little attention was paid to this group in East Asia area. During a field survey in South Korea, several Chlorociboria specimens were collected. Based on morphological and phylogenetic analyses, three species of Chlorociboria were reported, including one new record in South Korea and one new record in Jeju Island. The key to the species of Chlorociboria from South Korea is provided.

Antimicrobial Activity of the Extracts of Forsythia suspensa and Dendranthema indicum

  • Li, Xing-Quan;Zhang, Xin-Feng;Lee, Kyu-Seung
    • Journal of Applied Biological Chemistry
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    • v.48 no.1
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    • pp.29-31
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    • 2005
  • Antimicrobial activities of extracts of Forsythia suspensa fruits and Dendranthema indicum buds and flowers against bacteria; Escherichia coli, Staphyloccus aureus, and Bacillus subtilis, and fungi; Aspergillus flavusn, Rhizopus stolonifer, Penicillium citrinum, Aspergillus niger, and Saccharomyces carlsbergensis, were investigated. The plants were extracted with 70% ethanol and the extracts were used for antimicrobial activity assay. All extracts exhibited significant inhibition activity against microorganisms at concentrations ranged from 1.66 to $100\;{\mu}l/ml$. The inhibition activity by the extract of D. indicum buds was stronger than by the extract of F. suspensa fruits and D. indicum flowers. D. indicum buds showed antimicrobial activity against S. aureus which was comparable to other medicinal plants. F. suspensa fruits and D. indicum flowers was suggested to be valuable sources as antimicrobial ingredients in food industry.

Antibacterial effect of naturally occurring unsaturated fatty acids from Prunus japonica against Propionibacterium acnes

  • Sultan, Md Zakir;Lee, Ki-Moo;Moon, Surk-Sik
    • Advances in Traditional Medicine
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    • v.9 no.1
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    • pp.90-96
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    • 2009
  • The antibacterial activity-guided fractionation of the MeOH extract of seeds of traditional medicinal plant Prunus japonica resulted in the isolation of linoleic acid and cis-11-eicosenoic acids, and their methyl esters. Linoleic acid inhibited the growth of Propionibacterium acnes, the acne-causing anaerobic bacterium, but cis-11-eicosenoic acid, methyl linoleate, and cis-11-eicosenoate were found to be inactive. Together with isolated linoleic acid, authentic saturated and unsaturated fatty acids were also tested against P. acnes with other bacteria and fungi. Most of the unsaturated fatty acids possessed anti-acne (MIC $16-128{\mu}g/mL$) and antimicrobial properties.

Peroxidase-mediated Formation of the Fungal Polyphenol 3,14'-Bihispidinyl

  • Lee, In-Kyoung;Yun, Bong-Sik
    • Journal of Microbiology and Biotechnology
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    • v.18 no.1
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    • pp.107-109
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    • 2008
  • Medicinal fungi, Phellinus linteus and Inonotus xeranticus, produce a cluster of yellow pigment in their fermentation broth that acts as an important element of biological activity. The pigment is composed of diverse polyphenols with a styrylpyrone moiety, mainly hispidin and its dimers, 3,14'-bihispidinyl, hypholomine B, and 1,1-distyrylpyrylethan. Although dimeric hispidins were proposed to be biosynthesized from two molecules of monomer via oxidative coupling by ligninolytic enzymes, laccase and peroxidase, the details of this process remain unknown. In this preliminary study, we attempted to achieve enzymatic synthesis of the hispidin dimer from hispidin by using commercially available horseradish peroxidase (HRP). Consequently, a hispidin dimer, 3,14'-bihispidinyl, was synthesized, whereas the other dimers, hypholomine B and 1,1-distyrylpyrylethan, were not produced. This result suggested that the oxidative coupling at the C-3 and C-14' positions of hispidins was dominant in the process of dimerization by HRP, and indicated that additional catalysts or substrates would be needed to synthesize other hispidin dimers present in the fungal metabolite.

Production and Characterization of Chitosan from Ginseng-Steaming Effluents by Mucor miehei

  • Kim, Jae-Ho;Lee, Ki-Sung;Kim, Na-Mi;Lee, Jong-Soo
    • Journal of Microbiology and Biotechnology
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    • v.12 no.5
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    • pp.760-765
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    • 2002
  • Mucor miehei KCTC 6011, which grew successfully in ginseng-steaming effluents and produced a large amount of chitosan efficiently, was selected from various fungi. Approximately 120 mg of chitosan per g-dry mycelium was maximally produced in 84 h at $25^{\circ}C$ when grown in the ginseng-steaming effluent (pH 8.0) supplemented with 0.5% yeast extract and 0.002% CuSO$_4$. Chitosan produced by Mucor miehei KCTC 6011 was identified by the IR-spectra to have deacety lated approximately 56%. Viscosity and molecular weight of the chitosan were 80 cps and $1.07\times10^3$ kDa, respectively. The chitosan at 1.5 mg/ml inhibited 73.9% of the mycelium growth of Rhizoctonia solani in 60 h.

Taxonomical Studies on Korean Higher Fungi(I)

  • Park, Wan-Hee;Kim, Tae-Hee;Kim, Byong-Kak;Ro, Ihl-Hyeob
    • Korean Journal of Pharmacognosy
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    • v.16 no.2
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    • pp.61-64
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    • 1985
  • To find new species of Basidiomycetes in Korea, 15 species were collected in the medicinal plants garden of Sook Myung Women’s University during the period from May to November, 1983. Of them, 11 species were identified and classified into 10 genera and seven families. Among these species, Macrocystidia cucumis(Pers. ex Fr.) Heim and Melanoleuca verrucipes (Fr.) Singer were found to be unrecorded species in Korea. Also their two genera are newly found ones in Korea. They were named 'Bahm Saek Min $B\breve{u}s\breve{u}t$' and 'Hin Bol Lock $B\breve{u}s\breve{u}t$' respectively and their characteristics were here reported.

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Effect of Leaf and Stem Blight on Growth and Root Yield of Paeonia lactiflora Pallas (작약 지상부 고사가 뿌리의 생육과 수량에 미치는 영향)

  • Park, So-Deuk;Kim, Se-Jong;Kim, Jae-Chul;Kim, Ki-Jae;Shin, Jong-Hee;Choi, Boo-Sull
    • Korean Journal of Medicinal Crop Science
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    • v.5 no.3
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    • pp.206-210
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    • 1997
  • The pathogenic fungi associated with blight of leaf and stem in peony were leaf spot (Alternaria sp.), powdery mildew (Erysiphe aquilegiae) and rust (Cronartium flaccidum). The infection of leaf spot and powdery mildew begins from late April to midMay and rust was infected in early June. Blight time of aerial part in peony started from late May and the ratio of blight on leaf and stem was more than 50% in late Aug. Yields of root by the incidence time of blight of leaf and stem were 69.1% in late June, 65.4% in late July and $87.6{\sim}92.7$% in August and September. The number of root of more than 10mm in root diameter blighted in late June and July was much lower than in August, but the paeoniflorin content in the former was much higher than the latter.

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Effect of P Levels in Nutrient solution on the Propagation of Arvuscular Mycorrhizal Funfi in Aeroponics (분무경재배에 의한 arbuscular 균근균 증식에서 양액내 인산농도의 영향)

  • Kim, Young-Ju;Jin, Seo-Yeong;Cho, Ja-Yong;Kim, Kil-Yong;Cha, Gyu-Seok;Soh, Bo-Kyoon
    • Korean Journal of Soil Science and Fertilizer
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    • v.37 no.5
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    • pp.350-355
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    • 2004
  • This experiment was carried out to screen the aeroponically grown host plants suitable for the mass propagation of arbuscular mycorrhizal fungi (AMF) inoculum and clarify the effect of P levels in nutrient solution on the growth of aeroponically grown sweet potato (Ipomoea batatas L.), AMF infection, and mass propagation of mycorrhizal spores, etc. Amount of biomass of host plant became higher, as the P levels in nutrient solutions increased from 5 to 20 M. AMF infection rates in mycorrhizal roots increased in higher P levels in nutrient solution, and decreased in lower parts of mycorrhizal roots by about 18.6-26.0%. About 586 mycorrhizal spores per 1 g fresh root were formed at 16 weeks after inoculation of mycorrhizal inoculum. Total of 830,479 mycorrhizal spores were propagated in each plot.

Effect of various chromatographic terpenoid fractions of Luffa cylindrica seeds on in-vitro antimicrobial studies

  • Nagarajan, K.;Saxena, Pallavi;Mazumder, Avijit;Ghosh, L.K.;Devi, G. Uma
    • Advances in Traditional Medicine
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    • v.10 no.1
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    • pp.21-28
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    • 2010
  • The objective of the present investigation is to evaluate the antimicrobial potency of the terpenoid fractions isolated from Luffa cylindrica seeds against various pathogenic microbes. The seeds were powdered and extracted with methanol in soxhlet appratus based on phytochemical screening. Three terpenoid components were isolated by column chromatography and identified by thin layer chromatography and chemical analysis which were designated as ${LCSF_4}^*$, ${LCSF_6}^*$ & ${LCSF_8}^*$ respectively. Disc diffusion method was employed to determine the antimicrobial effectiveness of test compounds I, II and III $({LCSF_4}^*,\;{LCSF_6}^*\;&\;{LCSF_8}^*)$ against 6 microbial species viz., Staphylococcus (S.) aureus, Bacillus (B.) subtilis, Escherichia (E.) coli, Pseudomonas (P.) aeruginosa, Candida (C.) albicans and Aspergillus niger. The disc was saturated with $100{\mu}l$ of each compound, allowed to dry and introduced on the upper layer of seeded agar plate. The plates were incubated overnight at $37^{\circ}C$. Microbial growth was determined by measuring the zonal inhibition diameters. Compound I showed maximum potency against gram positive S. aureus (21 mm) in comparison with standard ciprofloxacin (38 mm), whereas the same compound was completely devoid of activity against both the fungi tested. Compound II was found to be highly sensitive against both the gram negative E. coli (20 mm) and P. aeruginosa (22 mm). Compound II was found to exhibit maximum potency against the fungi C. albicans (15 mm) and A. niger (20 mm). Compound III was found to be very effective against both the gram positive S. aureus (20 mm) and B. subtilis (15 mm) respectively.