• Biomedical Science Letters
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• v.17 no.1
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• pp.79-84
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• 2011

Photodynamic therapy consists of a photosensitizer, suitable light source and oxygen. The excitation of the photosensitizer at a cancer mass results in oxidation which would ultimately reduce the mass via apoptosis. Millimeter wave (MMW) therapy has also been known to be effective on cancer cell mass reduction, human cell regeneration and immunity enhancement among the Russian clinicians and scientists. In the present study, the two modalities were combined to achieve synergistic effects while reducing the administration dosage of the photosensitizer, photogem, thus minimizing the side effects. The CT-26 adenocarcinoma cell mass was implanted on mice and the tumors were exposed to a simple MMW irradiation or a combined treatment of MMW and PDT. The treatments continued for 4 weeks and the size of the tumor was measured continuously. The significant therapeutic result of MMW was not found during 4 weeks, preferably more cancer recurrence possibility after MMW irradiation was observed. The results of this study suggest that the combination of MMW irradiation and photodynamic treatment should not be recommended. The result of the MMW treatment alone, however, displayed suppressive effect on cancer cell proliferation for both in vitro and in vivo. The results of the present study suggest that the millimeter wave therapy deserves a further study.

• Clinical Endoscopy
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• v.56 no.6
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• pp.681-692
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• 2023

In general, gastroesophageal reflux disease (GERD) is diagnosed clinically based on typical symptoms and/or response to proton pump inhibitor treatment. Upper gastrointestinal endoscopy is reserved for patients presenting with alarm symptoms, such as dysphagia, odynophagia, significant weight loss, gastrointestinal bleeding, or anorexia; those who meet the criteria for Barrett's esophagus screening; those who report a lack or partial response to proton pump inhibitor treatment; and those with prior endoscopic or surgical anti-reflux interventions. Newer endoscopic techniques are primarily used to increase diagnostic yield and provide an alternative to medical or surgical treatment for GERD. The available endoscopic modalities for the diagnosis of GERD include conventional endoscopy with white-light imaging, high-resolution and high-magnification endoscopy, chromoendoscopy, image-enhanced endoscopy (narrow-band imaging, I- SCAN, flexible spectral imaging color enhancement, blue laser imaging, and linked color imaging), and confocal laser endomicroscopy. Endoscopic techniques for treating GERD include esophageal radiofrequency energy delivery/Stretta procedure, transoral incisionless fundoplication, and endoscopic full-thickness plication. Other novel techniques include anti-reflux mucosectomy, peroral endoscopic cardiac constriction, endoscopic submucosal dissection, and endoscopic band ligation. Currently, many of the new endoscopic techniques are not widely available, and their use is limited to centers of excellence.

• Journal of Korean Neurosurgical Society
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• v.50 no.3
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• pp.157-165
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• 2011

Objective : We have introduced a method of characterization of invading glioma cells by using molecular analysis of marginal invading tumor cells and molecular profiles of glioma tumor margin. Methods : Each of tumor core and marginal tissues was obtained in 22 glioma patients. Tumor core cells and marginal cells from each glial tumor were collected by laser capture microdissection or intraoperative microdissection under the operating microscope. Expression of MMP-2, MMP-9, CD44 and RHAMM mRNA by invading glioma cells compared with tumor core was confirmed by realtime-PCR of twenty-four glioma specimens. Clinical data also were reviewed for invasion and recurrence pattern of the gliomas radiologically and invasive rim pattern microscopically. Results : Overall results of the molecular analysis showed that relative overexpression of MMP-2, MMP-9 and RHAMM were noted at the invasive edge of human glioma specimens comparing to the tumor core but CD44 was highly expressed in the tumor core comparing to the margin. High marginal expression of MMP-2 and MMP-9 were noted in poorly ill-defined margin on the pathological finding. High marginal expression of CD44 and MMP-2 were demonstrated in the midline cross group on the radiological review, and that of RHAMM and MMP-2 were showed in the aggressive recurrence group. High expression of MMP-2 seems to be involved in the various invasion-related phenomenons. Conclusion : Up-regulation of MMP-2, MMP-9, CD44 and RHAMM was noted in invasive edge of gliomas according to the various clinical situations.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.4 no.3
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• pp.257-259
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• 2003

본 논문은 다이오드 레이저와 광 다이오드를 사용한 이중 빗살형 다이오드 레이저 비색 분광기를 개발하여 항균 성분의 양이온 계면 활성제로 널리 사용되고 있는 cetylpyridinium chloride(CPC)의 농도를 측정하였다 분광기의 안정도는 광원의 세기, 감도, 재현성을 측정한 예비 실험을 통하여 검증이 되었다 또한 상용화된 UV/VIS분광기와의 비교 결과를 나타내었다. 다이오드 비색 분광기는 3×10/sup -5/M에서 1.1×10/sup -4/M의 CPC 농도 범위에서 0.9635의 상관계수를 나타내었다. 이러한 결과는 CPC의 농도 분석을 위한 간편한 다이오드 레이저 비색 분광기 개발의 가능성을 나타내었다.

• Journal of International Society for Simulation Surgery
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• v.3 no.2
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• pp.49-59
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• 2016

3D printing is also known as additive manufacturing technique in which has been used in various commercial fields such as engineering, art, education, and medicine. The applications such as fabrication of tissues and organs, implants, drug delivery, creation surgical models using 3D printer in medical field are expanding. Recently, 3D printing has been developing for produce biomimetic 3D structure using biomaterials containing living cells and that is commonly called "3D bio-printing". The 3D bio-printing technologies are usually classified four upon printing methods: Laser-assisted printing, Inkjet, extrusion, and stereolithograpy. In the bio-printing, bio-inks (combined hydrogels and living cells) are as important components as bio-printing technologies. The presence of various types of bioinks, however, in this review, we focused on the bio-inks which enables bioprinting efficacy using hydrogels with living cells.

• Asian Pacific Journal of Cancer Prevention
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• v.16 no.14
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• pp.5589-5594
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• 2015

Cystitis and proctitis are defined as inflammation of bladder and rectum respectively. Haemorrhagic cystitis is the most severe clinical manifestation of radiation and chemical cystitis. Radiation proctitis and cystitis are major complications following radiotherapy. Prevention of radiation-induced haemorrhagic cystitis has been investigated using various oral agents with minimal benefit. Bladder irrigation remains the most frequently adopted modality followed by intra-vesical instillation of alum or formalin. In intractable cases, surgical intervention is required in the form of diversion ureterostomy or cystectomy. Proctitis is more common in even low dose ranges but is self-limiting and improves on treatment interruption. However, treatment of radiation proctitis is broadly non-invasive or invasive. Non-invasive treatment consists of non-steroid anti-inflammatory drugs (NSAIDs), anti-oxidants, sucralfate, short chain fatty acids and hyperbaric oxygen. Invasive treatment consists of ablative procedures like formalin application, endoscopic YAG laser coagulation or argon plasma coagulation and surgery as a last resort.

• Journal of the Korean Society of Laryngology, Phoniatrics and Logopedics
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• v.21 no.1
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• pp.54-56
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• 2010

Amyloidosis is a diverse group of disorders that share the deposition of amyloid protein in various extracellular tissues. Systemic amyloidosis may involve any body part and about 9-15% of it is localized. In the head and neck, the larynx is known as most common involved site. A histological diagnosis of amyloidosis is necessary to establish a definite diagnosis. Conservative surgery can be done for relieved airway obstruction or restored voice. We present three cases of patients with laryngeal amyloidosis that was treated with laryngeal microscopic surgery with laser.

• Current Optics and Photonics
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• v.3 no.6
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• pp.485-495
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• 2019

Photobiomodulation (PBM) using organic light emitting diodes (OLEDs) surface light sources have recently been claimed to be the next generation of PBM light sources. However, the differences between light emitting diodes (LEDs) and OLED mechanisms in vitro and in vivo have not been well studied. In vivo mouse models were used to investigate the effects of OLED irradiation on cellular function and cutaneous wound healing compared to LED irradiation. Mice in the LED- and OLED-irradiated groups were subjected to irradiation with 6 J/㎠ LED and OLED (630 nm), respectively, for 14 days after wounding, and some mice were sacrificed for the experiments on days 3, 7, 10, and 14. To evaluate wound healing, we performed hematoxylin-eosin and Masson's trichrome staining and quantified collagen density by computerized image analysis. The results showed that the size of the wound, collagen density, neo-epidermis thickness, number of new blood vessels, and number of fibroblasts and neutrophils was significantly influenced by LED and OLED irradiation. The tissue levels of interleukin (IL)-β, IL-6 and tumor necrosis factor (TNF)-α were investigated by immunohistochemical staining. LED and OLED irradiation resulted in a significant increase in the tissue IL-β and IL-6 levels at the early stage of wound healing (P < 0.01), and a decrease in the tissue TNF-α level at all stages of wound healing (P < 0.05), compared to the no-treatment group. The expression levels of the genes encoding vascular endothelial growth factor and transforming growth factor-beta 1 were significantly increased in LED and OLED-irradiated wound tissue at the early stage of wound healing (P < 0.01) compared to the no-treatment group. Thus, OLED as well as LED irradiation accelerated wound healing by modulating the synthesis of anti-inflammatory cytokines and the expression levels of genes encoding growth factors, promoting collagen regeneration and reducing scarring. In conclusion, this suggests the possibility of OLED as a new light source to overcome the limitations of existing PBMs.

• Journal of Oral Medicine and Pain
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• v.26 no.4
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• pp.395-406
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• 2001

지난 수십년동안, 다양한 레이저기기를 이용하여 치아 경조직에 대한 응용 실험을 수행되었다. 그러나, 발생한 주요한 문제는 열발생과 플라즈마 형성과 같은 부작용이 지속된다는 점이었다. 실망스러운 실험결과에 따라 치아 삭제에 대한 레이저의 응용에 대한 연구가 거의 진행되지 못하였다. 본 연구의 목적은 국내에서 처음으로 개발된 얼비움레이저기기를 사용하여 다양한 에너지 수준과 다양한 펄스빈도에서 법랑질의 삭제율을 비교하고자 하였다. 본 연구에 사용된 레이저기기는 국내에서 최초로 개발된 $2.94{\mu}m$ 펄스를 $250{\mu}s$ 방출하는 얼비움야그레이저 기기이다. 기기의 에너지는 20 mJ에서 350 mJ까지 다양하게 방출하며, 최대 출력은 3.5 W이다. 22개의 치아를 에폭시 레진에 포매하고 경화하였다. 치아의 법랑질을 회전 다이아몬드 칼을 흐르는 물과 함께 사용하여 협측 또는 설측으로 평탄하게 삭제하였다. 제작된 법랑질표면은 6개의 구획으로 구분하여 에너지 수준과 펄스빈도에 따라 조사하였다. 본 연구에서 사용된 표본 수는 132개이며, 삭제율을 조사하기 위한 모든 표본은 22개의 실험 군으로 분류하였다. 실험결과는 다음과 같다. 1. 에너지 수준간에는 유의한 삭제율의 차이가 있었다. 2. 40 및 80 mJ로 조사한 경우에는 펄스빈도 간에 차이가 없었지만, 120 mJ의 출력으로 동일한 에너지를 조사한 경우에는 20 Hz가 40 또는 80 mJ로 조사한 경우 보다 가장 삭제효과가 높았다. 3. 연속조사 한 경우 조사시간 경과에 따라 법랑질의 삭제율은 증가되지 않았다. 결론적으로 효과적으로 법랑질을 삭제하기 위해서는 적절한 에너지와 펄스의 레이저를 사용해야 하며, 또한 항상 정확한 초점거리를 유지해야 할 것으로 사료된다.

• Journal of the Korean Society of Laryngology, Phoniatrics and Logopedics
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• v.19 no.1
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• pp.11-15
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• 2008

Endoscopic laser cordectomy is known as an oncologically sound procedure for T1 and selected T2 glottic carcinoma ; it has comparable local control rate and better long-term laryngeal preservation rate when compared with those of radiotherapy. Even if results of the reported voice outcome studies after surgery or radiotherapy are diverse and controversial, resection deeper than the body layer of the vocal fold (type III, IV, V cordectomy) usually leads to aerodynamic insufficiency during phonation and results in poor voice quality. A keyhole defect or development of synechiae at the anterior commissure after type VI cordecomy may also result in unsatisfactory vocal outcome. However, many advances in phonosurgical techniques are reported to be successfully applied in the reconstruction of glottal defect that is subsequent to endoscopic laser cordectomy. In case of glottal insufficiency, voice restoration can be achieved by means of augmentation of the paraglottic space or medialization of the excavated vocal fold. Injection laryngoplasty with synthetic materials or autologous fat is gaining its popularity for restoring minor glottal volume defect because of its convenience. Laryngeal framework surgery, especially type I thyroplasty with premade implant systems or Gore-Tex, is most frequently used to correct larger glottic volume defect. In case of anterior commissural keyhole defect, additional procedure including laryngofissure may be required. For anterior commissural synechiae, laryngeal keel may be inserted for several weeks or mitomycin-C may be repeatedly applied after the division of adhesive scar to prevent restenosis. In this paper, current concepts and the authors' experiences of phonosurgical reconstruction of vocal function after endoscopic cordectomy will be introduced.