• Journal of Microbiology and Biotechnology
• /
• 제14권3호
• /
• pp.607-611
• /
• 2004

Glycinin, one of the predominant storage proteins in soybeans, was examined as to whether it could be used as a calcium-binding mediator after chemical phosphorylation and enzymatic hydrolysis. Glycinin is composed of six subunits. One of the proglycinin subunits $(A_{la}B_{lb})$ was overexpressed in E. coli to obtain nonphosphorylated proteins with homogeneity. To investigate the enhanced calcium-binding properties of the phosphopeptides, the proglycinin was purified, phosphorylated, and hydrolyzed with trypsin. The proglycinin expressed in E. coli was purified by ammonium sulfate precipitation, ion-exchange chromatography, and cryoprecipitation. Chemical phosphorylation by sodium trimetaphosphate was performed to obtain phosphorylated proglycinin. After the phosphorylation, one-dimensional isoelectric focusing gel electroanalysis confirmed the phosphorylation of the proglycinin. The phosphorylated peptides were then hydrolyzed with trypsin, followed by a binding reaction with calcium chloride. The calcium-bound phosphopeptides were finally separated using immobilized metal $(Ca^{2+})$ chromatography. Consequently, a limited tryptic hydrolysate of the isolated phosphopeptides exhibited an enhanced calcium-binding ability, suggesting the potential of glycinin phosphopeptides as a calcium-binding mediator with greater availability.

• The Korean Journal of Physiology and Pharmacology
• /
• 제15권4호
• /
• pp.245-249
• /
• 2011

Amphetamine, a synthetic psychostimulant, is transported by the dopamine transporter (DAT) to the cytosol and increases the exchange of extracellular amphetamine by intracellular dopamine. Recently, we reported that the phosphorylation levels of ezrin-radixin-moesin (ERM) proteins are regulated by psychostimulant drugs in the nucleus accumbens, a brain area important for drug addiction. However, the significance of ERM proteins phosphorylation in response to drugs of abuse has not been fully investigated. In this study, using PC12 cells as an in vitro cell model, we showed that amphetamine increases ERM proteins phosphorylation and protein kinase C (PKC) ${\beta}$ inhibitor, but not extracellular signal-regulated kinase (ERK) or phosphatidylinositol 3-kinases (PI3K) inhibitors, abolished this effect. Further, we observed that DAT inhibitor suppressed amphetamine-induced ERM proteins phosphorylation in PC12 cells. These results suggest that $PKC{\beta}$-induced DAT regulation may be involved in amphetmaine-induced ERM proteins phosphorylation.

• Proceedings of the Korea Contents Association Conference
• /
• 한국콘텐츠학회 2006년도 춘계 종합학술대회 논문집
• /
• pp.479-482
• /
• 2006

Mobile Telemedicine uses common technologies that provide a conduit for information exchange between physicians nurses and patients. In addition to patient records, medical professionals will obtain vital signs and other reference data through Ubiquitous Sensor networks(USN). In this paper about a design and implementation of RFID reader platform that received RFID tag information from patients tags, transmit the data to SBC(Single board computer) based on Intel PXA255 ARM CPU and SBC transmit the data to MySQL server in hospital using by Wireless Internet. his system that based on Embedded Linux is composed of RFID module, ARM processor, RS-232 interface, and Wireless LAN. This paper also provides a brief overview and concept of RFID reader, and proposes the design and implementations of the RFID reader platform used QT and MySQL based on Embedded Linux.

• Journal of Chest Surgery
• /
• 제23권6호
• /
• pp.1049-1056
• /
• 1990

The OXYREX hollow fiber membrane oxygenator developed by joint work of KIST and Green Cross Medical company has been evaluated by experimental investigation and clinical application, In this oxygenator gas exchanges occur through small pores of 0.1pm size which are distributed on 70% of surface of polypropylene hollow fiber. The Oxyrex membrane oxygenator consists of 36 thousand hollow fibers and it has 3.3m2 of gas exchange surface. The Oxyrex membrane oxygenator has unique blood flow path: blood enters the oxygenator passes between the hollow fibers and exits through outlet ports, that provides low transmembrane pressure drop. In the animal experiment and in vitro investigations of Oxyrex oxygenator, it showed low transmembrane pressure difference, effective heat exchanger performance, stable gas transfer function and less blood trauma. The Oxyrex oxygenator been used from March, 1990, to October, 1990, in 40 patients undergoing open heart operations. In the clinical applications of Oxyrex, adequate oxygenation[PaO2, 283$\pm$70mmHg] and carbon dioxide removal[PaCO2, 27\ulcorner6mmHg]were maintained under the condition of FiO2: below 0.6, Hct; 25%, perfusion flow; 2.4 L/min, gas flow: 2.1 L/min. During maximum 365 minutes of cardiopulmonary bypass[CPB] time period, the Oxyrex oxygenator maintained stable condition of PaO2, PaCO2 respectively and it also kept low plasma hemoglobin level. The complement proteins C3 and CH50 were not significantly changed pre to post CPB. There were no complications related to the oxygenator during and after the CPB.

• Journal of Acupuncture Research
• /
• 제20권4호
• /
• pp.85-92
• /
• 2003

목적 : 허혈시 발생되는 저산소중 상태에서는 세포독성을 유발한다고 알려져 있으나 정확한 기전은 아직 규명되지 않았다. 본 연구에서는 뇌허혈로 인한 세포독성의 기전을 유전자 발현을 통하여 살펴보고자 하였다. 방법 : 본 실험에서는 BV-2 microglia 세포주에 12시간 동안의 저산소 상태에서의 유전자 발현을 분석하기 위하여 마이크로에레이를 시행하였다. 결과 : 저산소 상태에서는 정상에 비하여 cathepsin F, growth factor independent 1, calcitonin/calcitonin-related poly, leucine-rich repeat LGI family membrane, dublecortin, cyclohydrolase 1, Ia-associated invariant chain, carbohydrate kinase-like과 erythrocyte protein band 4.1-like 3 등의 유전자 발현이 3배 이상 증가하였다. 한편 neuronal guanine nucleotide exchange factor, Bcl-2-related ovarian killer protein, chemokine (C-X-C motif) ligand 5, RNA binding motif protein 3, interleukin 2 receptor, alpha chain, crystallin zeta, cytochrome P450 subfamily IV B, asparagine synthetase과 moesin 등의 유전자 발현은 0.2배 이하로 감소하였다. 결론 : 이상의 결과는 저산소중에 관여하는 유전자 및 저산소중과 관련된 뇌경색 등의 질환의 기전을 밝히는데 기초적 자료로 이용될 수 있을 것이다.

• Journal of Korean Academy of Nursing Administration
• /
• 제3권1호
• /
• pp.83-92
• /
• 1997

This study was designed to evaluate the current orientation and further to develop an effective orientation curriculum of new nurses by analyzing questionraire taken from 45 numbers of all nurses who have worked for 18 months below at one hospital located in C city on July, 1995. The results obtained are summarized as follows. 1. The subjects showed the high necessity of "emergency nursing", "cardiopulmunary resuscitation", "disinfection and infection" and "interpersonal relationship" in order, but relatively low necessity of "doctor supports" and "medical insurance affair" for the practical orientation. Therefore, the orientation should provide prepondently an education Which they really need, on the basis of their education experiences and/or requirements. 2. Practical training, individual teaching and performance will be an effective orientation more than theorical education. The educator should be selected from unit based persons being capable of providing technical education to trainee. Also, in order to develop the teaching method and to improve the corresponding ability, a special program is required for educators. 3. It will be desirable that task training is given at least one month before working at their unit. In addition, orientation schedule should be made to concentrate trainee on their task training fully. 4. The subjects showed that half of them had spent four to six months for work adaptation. A meeting of new nurses may be helpful not only to acclimate themselves to new circumstances. but also to provide an exchange of views and an emotional relationship. Furthermore, unit based staffs should exerts efforts to maintain the educational circumstances and warm concern for new nurse's adaptation.

• Journal of Korean Academy of Nursing Administration
• /
• 제9권1호
• /
• pp.5-18
• /
• 2003

Purpose : For improvement of nursing services in ambulatory unit, ambulatory nursing activity was identified and the required time by nursing activity was measured. Method : The data were collected from 4 tertiary hospitals and 2 secondary hospitals. The method of data collection was used the self-reporting method. The 123 nurse-work days and 137 nurse-aid-work days in 6 hospitals were analyzed. Results : As a result, 11 ambulatory nursing domains and 79 ambulatory nursing activities were confirmed. Above 50% of subjects performed the general outpatient affair domain. The percentage of total time spent in each domain were as follows : general outpatient affair domain, 58.3%, patient education ${\cdot}$ counseling domain, 10.8%, therapeutic care domain, 8.6% and so on. And the average hours were measured by activity and domain. Conclusion : For the improvement of ambulatory nursing care, the strategies that keep the legal standard of number of ambulatory nurse and exchange the nursing-aid for the nurse is needed. And it is also needed to standardize the ambulatory nursing services.

• The Journal of the Korea institute of electronic communication sciences
• /
• 제2권4호
• /
• pp.244-249
• /
• 2007

HL7 is well-Known standard protocol for text data generated in hospital information systems. In this paper, we have to design to obtain useful vital sign information, which is generated at data receiver modulor of HIS, that is offered by the central monitor. Vital sign informations of central monitor is composed of the row data of several bedsite patient monitors. We are willing to maintain vital sign information of real time and continuity that is generated from the bedsite patient monitor. It is able to apply to remote medical examination and treatment. we proposed integration method between vital sign database systems and hospital information systems. Through the proper exchange and management of patient vital sign information, real time vital sign information management will offer better workflow to all hospital employee.

• International journal of advanced smart convergence
• /
• 제5권1호
• /
• pp.1-7
• /
• 2016

Recently, introduced a cloud computing technology to the IT industry, smart phones, it has become possible connection between mobility terminal such as a tablet PC. For dissemination and popularization of movable wireless terminal, the same operation have focused on a viable mobile cloud in various terminal. Also, it evolved Wireless Sensor Network(WSN) technology, utilizing a Body Sensor Network(BSN), which research is underway to build large Ubiquitous Sensor Network(USN). BSN is based on large-scale sensor networks, it integrates the state information of the patient's body, it has been the need to build a managed system. Also, by transferring the acquired sensor information to HIS(Hospital Information System), there is a need to frequently monitor the condition of the patient. Therefore, In this paper, possible sensor information exchange between terminals in a mobile cloud environment, by integrating the data obtained by the body sensor HIS and interoperable data DBaaS (DataBase as a Service) it will provide a base of mBodyCloud System. Therefore, to provide an integrated protocol to include the sensor data to a standard HL7(Health Level7) medical information data.

• Journal of Korean Clinical Nursing Research
• /
• 제18권1호
• /
• pp.99-110
• /
• 2012

Purpose: The purpose of this study was to examine the puncture incidence of surgical gloves in an operational setting. Methods: The 277 surgical gloves were used collecting at one general hospital located in Seoul from February 1, 2011 to April 3. The data were analyzed by $x^2$ test, Fisher's exact test, and multiple logistic regression. Results: In the dental and orthopedic department, the puncture incidence were significantly higher than others. The time of wearing gloves demonstrated significant difference: The group 181-240 minutes had a significantly higher than those with under 90, 91-180, or over 240 minutes. The influencing factors were as follows. Compared to that of the 2nd assistants, the puncture incidence rates of the surgeons were 9.91 times, scrub nurses were 8.39 times higher respectively. The participants in work experience under 1 year showed a 4.58 times higher than those with over 7 years. In addition, compared to the puncture incidence rate of neurosurgery department, the 17.41 times in cardio-thoracic surgery, 13.89 times in dental surgery, 4.93 times in gynecology, and 4.97 times in orthopedics higher respectively. Conclusion: There is a need for training operational room personnels to occasionally exchange the gloves even during the procedure and to use double surgical gloves.