• 대한암한의학회지
• /
• 제20권2호
• /
• pp.5-11
• /
• 2015

Objective : The objective of this study was to investigate the anti-tumor activity of the complexed herbal formula, Haeamdan (HAD). Methods : Seven Cancer cell lines, LoVo, MCF-7, AGS, Sarcoma 180, HL-60, NCI-H69, LL/2, were prepared and the cytotoxicity was assessed by 3-(4,5-dimethylthiazol-2yl)-2,5-dephenyl tetrazolium bromide (MTT) assay. HAD was applied with various concentrations from 0.1 to 1.0 mg/ml to figure out the appropriate dosage. ICR male mice were intraperitoneally implanted with Sarcoma 180 and divided into 8 species for each group. Control group was treated with normal saline, positive control group was treated with cyclophosphamide 8mg/kg, and experimental group was treated with HAD 1g/kg. Results : Among seven cancer cell lines, HAD exhibited strong cytotoxic activities to followed four cancer cell lines, that is, Sarcoma 180, HL-60, NCI-H69, and LL/2. These cytotoxic activity was expressed under 0.50 mg/ml of IC50 under 0.1~1mg/ml of OBW. When Sarcoma 180 cancer cell was implanted in ICR male mice and treated with the HAD, HAD prolonged the median overall survival for 3.6 days, from 17.5 to 21.1 days. Conclusion : HAD showed strong cytotoxicity to the cancer cells, Sarcoma 180, HL-60, NCI-H69, on in vitro study and it showed anti-tumor activity in vivo with the peritoneal cancer mice by prolonging the median survival for 3.6 days. Further researches would be expected to support the anti-tumor efficacy of HAD.

• Radiation Oncology Journal
• /
• 제32권1호
• /
• pp.7-13
• /
• 2014

Purpose: To evaluate the results of postoperative radiotherapy in patients with extra-hepatic bile duct cancer (EHBDC) and identify the prognostic factors for local control and survival. Materials and Methods: Between January 2001 and December 2010, we retrospectively reviewed the cases of 70 patients with EHBDC who had undergone curative resection and received postoperative radiotherapy. The median radiation dose was 50.4 Gy (range, 41.4 to 54 Gy). The resection margin status was R0 in 30 patients (42.9%), R1 in 25 patients (35.7%), and R2 in 15 patients (21.4%). Results: The 5-year rates of overall survival (OS), event-free survival (EFS), and locoregional control (LRC) for all patients were 42.9%, 38.3%, and 61.2%, respectively. The major pattern of failure was distant relapses (33 patients, 47.1%). A multivariate analysis showed that the postradiotherapy CA19-9 level, radiation dose (${\geq}50$ Gy), R2 resection margins, perineural invasion, and T stage were the significant prognostic factors for OS, EFS, and LRC. OS was not significantly different between the patients receiving R0 and R1 resections, but was significantly lower among those receiving R2 resection (54.6%, 56.1%, and 7.1% for R0, R1, and R2 resections, respectively). Conclusion: In patients with EHBDC who had undergone curative resection, a postoperative radiotherapy dose less than 50 Gy was suboptimal for OS and LRC. Higher radiation doses may be needed to obtain better LRC. Further investigation of novel therapy or palliative treatment should be considered for patients receiving R2 resection.

• Asian Pacific Journal of Cancer Prevention
• /
• 제13권12호
• /
• pp.6129-6132
• /
• 2012

Objectives: To evaluate the feasibility and efficacy of simultaneous accelerated radiation therapy (SMART) and concurrent weekly paclitaxel in the treatment of locally advanced nasopharyngeal carcinoma. Methods: Forty-one patients with pathologically confirmed nasopharyngeal carcinoma were treated by SMART with concurrent weekly paclitaxel. Daily fraction doses of 2.5 Gy and 2.0 Gy were prescribed to the gross tumor volume (GTV) and clinical target volume (CTV) to a total dose of 70 Gy and 56 Gy, respectively. Paclitaxel of $45mg/m^2$ was administered concurrently with radiation therapy every week. Adjuvant chemotherapy was given four weeks after the completion of the radiotherapy (RT) if the tumor demonstrated only a partial response (PR). Results: All patients completed the radiotherapy (RT) course. Adjuvant chemotherapy was administered to 12 patients due to PR. The CR (complete remission) rate was 82.9% three months after RT. Thirty-nine (95.1%) patients completed the concurrent weekly chemotherapy with paclitaxel, and two patients skipped their sixth course. Seven patients had a 15% dosage reduction at the fifth and sixth course due to grade 3 mucositis. The median follow-up was 30 (range, 14-42) months. The three-year overall survival (OS), metastases-free survival (MFS), and local control rates were 77.0%, 64.4%, and 97.6%, respectively. No correlation between survival rate and T or N stage was observed. Grade 3 acute mucositis and xerostomia were present in 17.1% and 7.1%, respectively. Conclusion: SMART with concurrent weekly paclitaxel is a potentially effective and toxicity tolerable approach in the treatment of locally advanced NPC.

• Radiation Oncology Journal
• /
• 제16권3호
• /
• pp.259-264
• /
• 1998

목적 :상악동암에서 수술 전 방사선 치료를 시행함으로써 안면 보존의 가능성이 어느 정도 되는지를 알아보고자 하였다. 대상 및 방법 : 1986년 6월부터 1990년 9월까지 상악동암으로 방사선치료를 받은 42례의 환자를 대상으로 후향적 분석을 시행하였다. 환자의 연령은 24세에서 75세로 중간값은 56세였으며, 남자 27예, 여자 15예였다. 병기별 분포는 T2 2예, T3 19예, T4 21예로 진단 당시 대부분 진행된 상태였다. 조직학적으로는 편평 상피암 38예, 미분화 세포암 1예, 이행 상피암 1예, 선양 낭종암 2예였다. 모든 환자에 대해 동측 부비동을 포함하는 쌍쐐기빔 방법의 방사선치료를 시행하였다. 방사선 치료선량은 50.4-70.2 Gy로 중간값은 70.2 Gy였다. 11례의 환자에 대해 방사선치료와 유도 화학요법을 병행하였다. 화학요법은 5-fluorouracil과 cisplatin으로 2회 시행하였다. 대상 환자의 추적기간은 3개월에서 92개월로 중간값은 16개월이었다. 방사선치료 후 6 내지 8개월 경과 후 전산화단층촬영을 실시하여 병소의 반응을 평가하였다. 완전관해가 된 경우는 계속 추적검진만 하였으며 병변이 의심되는 경우는 콜드웰-뤼크 수술을 시행하여 확인하였다. 병변이 확인된 경우는 상악동전절제술을 시행하였다. 결과 : 방사선 및 화학요법 시행 후 9예 (21.4$\%$)에서 완전관해를 보였고 33예 (88.6$\%$)에서 잔여병변이 확인되었다. 완전관해를 보인 9예의 환자 중 7예가 국소제어되었으며 2예에서 국소재발하였다. 잔여병변이 있는 33예의 환자 중 10예에서 근치적 수술이 가능하였고 그 중 2예에서 국소제어가 되었다. 전체 환자의 5년 생존율은 23.1$\%$였고 5년 무병생존율은 16.7$\%$였다. 방사선치료에 대한 반응 (P<0.05) 만이 생존율에 영향을 미치는 유의한 인자였다. 결론 : 방사선치료에 대한 반응만이 생존율에 영향을 미치는 유의한 인자였다. 전체적으로 7예(16.7$\%$) 에서 수술 전 방사선치료로 안면보존이 가능하였다.

• 한국약용작물학회지
• /
• 제28권5호
• /
• pp.339-346
• /
• 2020

Background: Cnidium officinale Makino have been used in traditional medicine in Northeast Asia. Although gamma-ray mutagenesis has been used to develop breeding resources with novel characteristics, research on the radiation sensitivity of C. officinale Makino is limited. Hence, the optimal gamma-ray dosage for mutation breeding in C. officinale Makino was investigated. Methods and Results: Seedstocks were exposed to doses of gamma rays (5 Gy - 50 Gy), and subsequently planted in a greenhouse. After 30 days of sowing, the survival rates and growth decreased rapidly at doses above 20 Gy, while all individuals died at 50 Gy. The median lethal dose (LD₅₀) was 25.65 Gy, and the median reduction doses (RD₅₀) for plant height, number of stems, and fresh weight were 12.81, 9.32, and 23.26 Gy, respectively. Post-irradiation levels of malondialdehyde (MDA), peroxidase (POD), and chlorophyll in the aerial parts of the plant were quantified using spectrophotometry. Relative to the controls, the levels of MDA and POD increased, while the level of chlorophyll decreased at doses ≥ 10 Gy, indicating cellular damage. Conclusions: A dose of 20 Gy was found to be optimal for mutation breeding in C. officinale Makino.

• 한국자원식물학회:학술대회논문집
• /
• 한국자원식물학회 2021년도 춘계학술대회
• /
• pp.27-27
• /
• 2021

Yacon [Samallanthus sonchifolius (Poepp. & Endl.) H. Robinson], a member of Compositae plants, has sweet taste and crisp texture. Unlike other Andean root crops such as potato and sweet potato, the cultivation area of yacon has increased recently, since it is known to have large content of fructooligosaccharides (FOS). Since there are no yacon varieties bred in Korea, we have been trying to create new genetic resources using gamma-ray. The optimal gamma-ray dosage for mutation breeding in yacon was investigated. Crown bud and green bud of yacon were exposed to doses of gamma rays from 20 Gy to 80 Gy, and subsequently planted in a greenhouse. After 50 days of sowing, the survival rates and growth decreased rapidly at doses above 40 Gy, while all of crown bud individuals died above 60 Gy. The median lethal dose (LD50) of crown bud and green bud was 22.4 and 36.6 Gy, and the median reduction doses (RD50) for plant height, fresh weights, and tuberous root weight were 20-40 Gy, respectively. A dose of 20-40 Gy was found to be optimal for mutation breeding in yacon. Considering the growth factors, the optimum doses were determined to be within the range of 20-40 Gy for the selection of useful mutant lines. M2-M3 mutant lines were obtained from 20-60 Gy gamma-ray-irradiated M1 plants through clonal propagation. These mutant lines will be used for the development of a new variety of yacon plant with high FOS and no crack tuberous root.

• Radiation Oncology Journal
• /
• 제13권1호
• /
• pp.9-18
• /
• 1995

목적 :상악동암의 적절한 치료 방법을 알기 위하여 후향적 분석을 시행하였다. 대상 및 방법 : 1986년 1월 부터 1992년 12월 까지 전남대학교 병원에서 치료받은 상악동에서 발생한 편평상피세포암 33명을 대상으로 하였다. 남자가 24명 여자가 9명이었고 연령의 중앙간은 55세였다. 1988년에 제시된 AJCC 병기 분류법에 따른 종양의 병기는 T2 1명, T3 10명, T4 22명이었으며 경부임파절 전이는 5명에서 관찰되었다(Nl:4명, N2b;1명). 치료방법에 따라 3군으로 분류하여 분석하였으며 첫 군(FAR 군으로 명명, 16명)은 수술전 경동맥 항암화학요법(5-FU, 평균 3078mg)과 방사선치료(평균 3433cGy) 및 비타민 A(50,000 IU. daily)를 병용하고 이어서 상악전적출술 과 술 후 방사선치료(평균 2351cGy)를 시행하였다. 방사선총량은 5255cGy였다. 둘째군(SR 군으로 명명, 7명)은 상악전적출술과 술후 방사선 치료(평균 5920cGy)를 시행하였다. 셋째군(R 군으로 명명, 6명)은 근치적 목적의 방사선 치료(평균 7164cGy)만 시행하였다. 생존율 분석에는 Kaplan-Meier법을 이용하였고 두 군간의 차이에 대한 검정은 Mantel-Cox법으로 하였다. 결과 : 국소종양 제어율은 2년에 FAR, SR, R군에서 각각 $100\%$, $50\%$, $27.7\%$로 나타났다. 무병생존율은 2년에 FAR, SR, R군에서 각각 $88.9\%$, $33.3\%$, $0\%$였다. 전체적인 생존율은 2년에 FAR, SR, R군에서 각각 $88.9\%$, $40\%$, $50\%$였다. FAR군과 SR군간에, 그리고 FAR군과 R군간에 국소종양 제어율, 2년 무병생존율, 2년 생존율에 있어서의 차이는 통계학적으로 유의하였다. 그러나 SR군과 R군간의 차이는 통계학적 유의성이 없었다. 결론 :수술전 경동맥 5-FU와 비타민 A 그리고 방사선 치료를 병용 치료하여 종양의 부분적 관해를 유도한 후 상악전적출술과 추가적인 방사선 치료를 시행한 FAR군이 상악전적출술후 방사선치료를 시행한 군이나 방사선 단독치료군보다 더 좋은 성적을 보였다. 앞으로 이에 대한 전향적 연구가 필요할 것으로 사료된다.

• Radiation Oncology Journal
• /
• 제33권2호
• /
• pp.109-116
• /
• 2015

Purpose: To analyze prognostic factors for locoregional recurrence (LRR), distant metastasis (DM), and overall survival (OS) in cervical cancer patients who underwent radical hysterectomy followed by postoperative radiotherapy (PORT) in a single institute. Materials and Methods: Clinicopathologic data of 135 patients with clinical stage IA2 to IIA2 cervical cancer treated with PORT from 2001 to 2012 were reviewed, retrospectively. Postoperative parametrial resection margin (PRM) and vaginal resection margin (VRM) were investigated separately. The median treatment dosage of external beam radiotherapy (EBRT) to the whole pelvis was 50.4 Gy in 1.8 Gy/fraction. High-dose-rate vaginal brachytherapy after EBRT was given to patients with positive or close VRMs. Concurrent platinum-based chemoradiotherapy (CCRT) was administered to 73 patients with positive resection margin, lymph node (LN) metastasis, or direct extension of parametrium. Kaplan-Meier method and log-rank test were used for analyzing LRR, DM, and OS; Cox regression was applied to analyze prognostic factors. Results: The 5-year disease-free survival was 79% and 5-year OS was 91%. In univariate analysis, positive or close PRM, LN metastasis, direct extension of parametrium, lymphovascular invasion, histology of adenocarcinoma, and chemotherapy were related with more DM and poor OS. In multivariate analysis, PRM and LN metastasis remained independent prognostic factors for OS. Conclusion: PORT after radical hysterectomy in uterine cervical cancer showed excellent OS in this study. Positive or close PRM after radical hysterectomy in uterine cervical cancer correlates with poor prognosis even with CCRT. Therefore, additional treatments to improve local control such as radiation boosting need to be considered.