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http://dx.doi.org/10.7783/KJMCS.2020.28.5.339

Gamma-ray Irradiation on Radio Sensitivity in Cnidium officinale Makino  

Jeong, Jin Tae (Department of Herbal Crop Research, NIHHS, RDA)
Ha, Bo Keun (Division of Plant Biotechnology, Chonnam National University)
Han, Jong Won (Department of Herbal Crop Research, NIHHS, RDA)
Lee, Jeong Hoon (Department of Herbal Crop Research, NIHHS, RDA)
Lee, Sang Hoon (Department of Herbal Crop Research, NIHHS, RDA)
Oh, Myeong Won (Department of Herbal Crop Research, NIHHS, RDA)
Park, Chun Geon (Department of Herbal Crop Research, NIHHS, RDA)
Ma, Kyung Ho (Department of Herbal Crop Research, NIHHS, RDA)
Chang, Jae Ki (Department of Herbal Crop Research, NIHHS, RDA)
Kim, Sang Hoon (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute)
Kim, Jin Baek (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute)
Kang, Si Yong (Department of Horticulture, College of Industrial Sciences, Kongju National University)
Ryu, Jai Hyunk (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute)
Publication Information
Korean Journal of Medicinal Crop Science / v.28, no.5, 2020 , pp. 339-346 More about this Journal
Abstract
Background: Cnidium officinale Makino have been used in traditional medicine in Northeast Asia. Although gamma-ray mutagenesis has been used to develop breeding resources with novel characteristics, research on the radiation sensitivity of C. officinale Makino is limited. Hence, the optimal gamma-ray dosage for mutation breeding in C. officinale Makino was investigated. Methods and Results: Seedstocks were exposed to doses of gamma rays (5 Gy - 50 Gy), and subsequently planted in a greenhouse. After 30 days of sowing, the survival rates and growth decreased rapidly at doses above 20 Gy, while all individuals died at 50 Gy. The median lethal dose (LD50) was 25.65 Gy, and the median reduction doses (RD50) for plant height, number of stems, and fresh weight were 12.81, 9.32, and 23.26 Gy, respectively. Post-irradiation levels of malondialdehyde (MDA), peroxidase (POD), and chlorophyll in the aerial parts of the plant were quantified using spectrophotometry. Relative to the controls, the levels of MDA and POD increased, while the level of chlorophyll decreased at doses ≥ 10 Gy, indicating cellular damage. Conclusions: A dose of 20 Gy was found to be optimal for mutation breeding in C. officinale Makino.
Keywords
Cnidium officinale Makino; Gamma Irradiation; Radiation Damage; Optimal Dose; Mutation Breeding;
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Times Cited By KSCI : 4  (Citation Analysis)
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