• Journal of Embryo Transfer
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• v.31 no.3
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• pp.221-226
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• 2016

The aim of the present study was to assess the embryo development and survivability of post-thawed bovine embryos produced in vitro by addition of cysteine. The rates of metaphase II formation were not differed significantly among three groups(TCM199 73.8%, TCM199 with 0.3% cysteine 76.9%, TCM199 with 0.5% cysteine 83.8%, respectively). No difference of cleavage rate(70.6~74.6%) was seen among three culture medium(TCM199 70.6%, CR1aa 71.3%, SOF 74.6%) with 0.5M cysteine. however, Significantly(P<0.05) higher development rate into blastocyst stage by 0.5M cysteine addition was obtained in SOF medium(35.6%) than in TCM199(27.6%) or CR1aa(26.6%), however no significant differences in the cleavage rates were among three culture medium. After frozen the blastocysts cultured with 0.5M cysteine, The re-expansion rates were 61.3%~86.4% among groups, and hatching rates were 26.3%~46.9% among groups, the rates of re-expansion and hatching were significantly(P<0.05) higher in SOF medium(86.4% and 46.9%) than those in TCM199(61.3% and 26.3%) and CR1aa medium(87.1 and 44.4%). After thawing, the blastocyst re-expansion rate was significantly(P<0.05) higher in in vivo (87.1%) and in vitro (70.3%) embryos. In conclusion, our results demonstrate that supplementation of IVM and IVC medium with 0.5M cysteine improved the quality of in vitro production embryo and post- thawed embryo. Future studies comparing these media systems in well-designed trials should be performed.

• Applied Chemistry for Engineering
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• v.7 no.2
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• pp.299-307
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• 1996

The surface tension of PO added sodium poly(oxyethylene(EO), oxyisopropylene(PO)) dodecyl ether sulfate firstly were slightly lower than EO added sulfate in the concentration range of $10^{-6}{\sim}10^{-3}mol/{\ell}$. And they had lower critical micelle concentration ($10^{-4}{\sim}9{\times}10^{-5}mol/{\ell}$) than general anionic surfactants. The adsorptivity ($2.2{\times}10^{-10}mol/cm^2$) of sodium $(PO)_{10}(EO)_5$ dodecyl ether(compound of PO addition firstly) calculated by Gibbs' adsorption isotherm were higher than that of sodium $(EO)_{10}(PO)_5$, dodecyl ether(compound of EO addition firstly), but were lower than that of sodium dodecyl sulfate (${\Gamma}=3.2{\times}10^{-10}mol/cm^2$). These could be understood that the adsorption areas of compounds were very large because of their high molecular weight. Moreover, PO compounds showed better properties than EO compounds in foamability, emulsifying power for organics (n-hexane, benzene), detergency for the lard, tallow oil mixture and dispersability for iron oxide. It was interpreted in terms of surface properties of the PO compounds. These showed that the interfacial activity become higher when hydrophilic and hydrophobic portion existed in aggoromerated state respectively. The test results of emulsifying power for organics (n-hexane, benzene) showed better for benzene than n-hexane. Eight kinds of sodium (EO, PO) dodecyl ether derivatives showed irregular dispersibilities for polar iron oxide in water dispersed media.

• Clinical and Experimental Reproductive Medicine
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• v.31 no.4
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• pp.209-215
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• 2004

Objective: Embryonic stem (ES) cells could be differentiated into the specific cell types by alternation of culture condition and modification of gene expression. This study was performed to evaluate the differentiation protocol for mouse and human ES cells to insulin secreting cells. Methods: Undifferentiated mouse (JH-I) and human (Miz-hESI) ES cells were cultured on STO feeder layer, and embryoid bodies (EBs) were formed by suspension culture. For the differentiation, EBs were cultured by sequential system with three stage protocol. The differentiating ES cells were collected and marker gene expressions were analyzed by seIni-quantitative RT-PCR in each stage. Amount of secreted insulin levels in culture media of human ES cells were measured by human insulin specific RIA kit. Results: During the differentiation process of human ES cells, GATA-4, a-fetoprotein, glucose transporter-2 and Ngn-3 expression were increased whereas OctA was decreased progressively. Insulin and albuInin mRNAs were expressed from stage IT in mouse ES cells and from stage III in human ES cells. We detected 3.0~7.9 IlU/rnl secretion of insulin from differentiated human ES cells by in vitro culture for 36 days. Conclusion: The sequential culture system could induce the differentiation of mouse and human ES cells into insulin secreting cells. This is the fIrst report of differentiation of human ES cells into insulin secreting cells by in vitro culture with serum and insulin free medium.

• Journal of Environmental Science International
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• v.24 no.2
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• pp.197-205
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• 2015

This study was performed to identify the effect of fertilizer application and planting method on growth and yield of 'Seolhyang' strawberry during seedling raising. According to the concentration of fertilizer applied, the height of daughter plants was the highest at an EC of $0.8dS{\cdot}m^{-1}$. Leaf number and crown diameter were greatest at an EC of $0.6-0.8dS{\cdot}m^{-1}$. In the first measurement, root number was highest in non-fertilizer application, while root weight was heaviest in non-fertilizer application and EC $0.4dS{\cdot}m^{-1}$. The higher the concentration of fertilizer applied as culture media, the lower the growth rate. Thirty days after planting on the main field, plant height and number of new leaf were highest at an EC of $0.8dS{\cdot}m^{-1}$. However, no significant difference was found in leaf length and width and chlorophyll content according to fertilizer application. Marketable yield of 25 g or higher was greatest in EC $0.6dS{\cdot}m^{-1}$. In contrast, no significance was found in total marketable yield at an EC of $0.4-0.8dS{\cdot}m^{-1}$. Aconsistent pattern was exhibited in the growth of 1-5 harvesting flower clusters according to planting method. The length of leaf and flower cluster was short and chlorophyll content was low, when bed soil was removed 100% in harvesting of the first flower cluster. In all treatment, leaf length was shortened until harvesting of the second and third flower cluster, but rapidly lengthened in harvesting of the third and fourth flower clusters. Moreover, the length of flower cluster had a increasing tendency from harvesting of the third flower cluster. However, chlorophyll content was reduced continuously until harvesting of the fifth flower cluster, and was lowest in harvesting of the fourth flower cluster without removal of bed soil. Total yield was greatest in treatment of crown removal in bed soil between November and May. Late marketable yield between March and May was highest in treatment of 100% bed soil removal, followed by treatment of crown removal. Marketable yield of 25 g or higher was greatest in treatment of crown removal between December and February, while greatest in treatment of 100% bed soil removal between March and May.

• Tuberculosis and Respiratory Diseases
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• v.79 no.4
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• pp.282-288
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• 2016

Background: Tuberculosis (TB) is a major health problem, and accurate and rapid diagnosis of multidrug-resistant (MDR) and extended drug-resistant (XDR) TB is important for appropriate treatment. In this study, performances of solid and liquid culture methods were compared with respect to MDR- and XDR-TB isolate recovery and drug susceptibility testing. Methods: Sputum specimens from 304 patients were stained with Ziehl-Neelsen method. Mycobacterium tuberculosis (Mtb) isolates were tested for recovery on $L{\ddot{o}wenstein$-Jensen (LJ) medium and the BacT Alert 3D system. For drug susceptibility testing of Mtb, isolates were evaluated on M-KIT plates and the BacT Alert 3D system. Results: The recovery rates were 94.9% (206/217) and 98.2% (213/217) for LJ medium and the BacT Alert 3D system, respectively (kappa coefficient, 0.884). The rate of drug resistance was 13.4% for at least one or more drugs, 6.0% for MDR-TB and 2.3% for XDR-TB. M-KIT plate and BacT 3D Alert 3D system were comparable in drug susceptibility testing for isoniazid (97.7%; kappa coefficient, 0.905) and rifampin (98.6%; kappa coefficient, 0.907). Antibiotic resistance was observed using M-KIT plates for 24 of the total 29 Mtb isolates (82.8%). Conclusion: The liquid culture system showed greater reduction in the culture period, as compared with LJ medium; however, drug susceptibility testing using M-KIT plates was advantageous for simultaneous testing against multiple drug targets.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.20 no.10
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• pp.1031-1041
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• 2009

A small microstrip monopole antenna for macro-micro frequency tuning over multiple bands is presented. The meander-shape antenna is fabricated on a conventional printed circuit board(FR-4, $\varepsilon_r=4.4$ and tan $\delta=0.02$). The antenna operates over WiBro(2.3~2.4 GHz) and WLAN a/b(2.4~2.5 GHz/5.15~5.35 GHz) service bands with an essentially constant antenna gain within each service band. Two diodes, a PIN diode and a varactor, are embedded into the antenna for frequency reconfiguration. The PIN diode is used for frequency switching(macro-tuning) between 2 GHz and 5 GHz bands while the varactor is used for frequency tuning(micro-tuning) within the service bands, 2.3~2.5 GHz and 5.15~5.35 GHz. Unwanted resonances between the two frequency bands(2 GHz and 5 GHz) are suppressed by filling up the gaps between the meander lines. The antenna gain is essentially constant and higher than 2 dBi within each service band. The measured performance of the proposed antenna system suggests the macro-micro frequency tuning techniques be useful in reconfigurable wireless communication systems.

• Biotechnology and Bioprocess Engineering:BBE
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• v.10 no.4
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• pp.378-384
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• 2005

Vanillin is one of the world's principal flavoring compounds, and is used extensively in the food industry. The potential vanillin production of the bacteria was compared to select and clone genes which were appropriate for highly productive vanillin production by E. coli. The fcs (feruloyl-CoA synthetase) and ech (enoyl-CoA hydratase/aldolase) genes cloned from Amycolatopsis sp. strain HR104 and Delftia acidovorans were introduced to pBAD24 vector with $P_{BAD}$ promoter and were named pDAHEF and pDDAEF, respectively. We observed 160 mg/L vanillin production with E. coli harboring pDAHEF, whereas 10 mg/L of vanillin was observed with pDAHEF. Vanillin production was optimized with E. coli harboring pDAHEF. Induction of the fcs and ech genes from pDAHEF was optimized with the addition of 13.3 mM arabinose at 18 h of culture, from which 450 mg/L of vanillin was produced. The feeding time and concentration of ferulic acid were also optimized by the supplementation of $0.2\%$ ferulic acid at 18 h of culture, from which 500 mg/L of vanillin was obtained. Under the above optimized condition of arabinose induction and ferulic acid supplementation, vanillin production was carried out with four different types of media, M9, LB, 2YT, and TB. The highest vanillin production, 580 mg/L, was obtained with LB medium, a 3.6 fold increase in comparison to the 160 mg/L obtained before the optimization of vanillin production.

• Journal of the Korean Society of Food Science and Nutrition
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• v.39 no.9
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• pp.1257-1262
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• 2010

This study investigated the anti-diabetic effects of the mixed water extract (JDG 100) composed of Lycii Cortex, Acanthopanax senticosus and Cordyceps militaris on glucose-regulating key enzymes such as glucokinase (GK), acetyl-CoA carboxylase (ACC). In the current study, HepG2 cells were exposed to pathological condition such as hyperglycemic condition (4.5 g glucose/L) with JDG 100 and then experiments such as RT-PCR and Western blotting were carried out. JDG 100 treated cells increased to $168{\pm}0.04%$ and $182.4{\pm}0.03%$ in GK mRNA and protein expressions, respectively, compared to control. Treatment of the JDG 100 up-regulated ACC mRNA ($127.3{\pm}0.02%$) and protein ($126.7{\pm}0.24%$) of HepG2 cells in the high glucose media. These observations suggest that JDG 100 mixed water extract may have a potential as an anti-diabetic agent in type 2 diabetes mellitus.

• Journal of Korean Clinical Health Science
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• v.5 no.1
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• pp.790-802
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• 2017

Purpose. This study was designed to analyze recent trends in Children Art Therapy Program Intervention research in Korea and suggest future research directions in this area. Methods. Studies(29) selected from http://www.riss4u.net for last 15 year were used. They were analyzed by publication type, field and design of the study, study participants and outcome variables used in intervention studies. Results. 1. As for academic field, there are 15 art therapy academic journals(51.72%), which made up the largest proportion. 2. As for subjects of the study, there are 17 articles of Elementary (58.6%) education, which made up the largest proportion. As for the subjects of sex, 8 articles (27.59%) are for male students, 7 (24.14%) articles are for female students and 14 articles (48.28.%) are for both male and female students. 3. As for 7 articles of Art therapy(24.14%), 7 articles of Group Art therapy (24.14%) made up the largest proporton 4. As for the intervention study method according to the subjects of suicide intervention program, there are 15 articles of monoclonal Pre and post design (51.72%),which made up the largest proportion. 5. As for the sample size, there were 16.79 persons in the treatment group on average, 13.28 persons in the control group have average and the total persons were 10 on average. The treatment period was 12 weeks on average and the average number of treatment times was 18. The places of treatment were 3 schools (10.38%), which made up the largest proportion. 19. The results of the experimental study support the research hypothesis of all 29 programs. Conclusions. As the above, the studies on the art therapy intervention program for children are increasing but the subjects are overly weighted toward elementary school students. Although the content of the art therapy intervention program was varied, it was found that the development of the program using various art media which can induce the motivation of the child was lacking. In addition, the place of experimental mediation was concentrated on psychology center as 12(41.38%), indicating that there are not enough places to connect with community organizations such as schools and hospitals. The variables of experimental study were focused on psychological variables and it was found that there were insufficient application of various variables including coping method, social support, and physiological variables.

• Journal of the Korean Society of Groundwater Environment
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• v.1 no.2
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• pp.105-112
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• 1994

To investigate the migration behavior of contaminants in rocks. adsorption and diffusion experiments for Sr as a sorbing contaminant and for Cr-EDTA as a non-sorbing contaminant were carried out on granitic rocks. The Sr adsorption on separated minerals and crushed rocks tends to slightly increase with increasing pH. It also greatly decreases with the increase of ionic strength in NaCl solution. Among separated minerals, biotite and sericite have adsorbed much more amount of Sr than other rock-forming minerals, such as quartz, plagioclase, and potassic feldspar, because the specific surfaces and cation exchange capacities of phyllosilicates are generally much greater than those of the other rock-forming minerals. The intrinsic diffusion coefficients of Cr-EBTA for granitic rocks differ little from those of Sr. This indicates that they are independent of water-rock interactions. Experimental data show that the intrinsic diffusion coefficients are positively correlated with the porosities of the rocks. They are close to the theoretically predicted values, especially in pre-steady state diffusion region, with the increase of rock sample thickness.