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http://dx.doi.org/10.12750/JET.2016.31.3.221

Developmental and survivability according to cryopreservation of in vitro produced bovine embryos cultured by addition of Antioxident cysteine  

Cho, Sang-Rae (Hanwoo Research Institute)
Kang, Sung-Sik (Hanwoo Research Institute)
Kim, Ui-Hyung (Hanwoo Research Institute)
Kim, Si-dong (Hanwoo Research Institute)
Lee, Seok-Dong (Hanwoo Research Institute)
Jeon, Gi-jun (Hanwoo Research Institute)
Park, Chang-Seok (Yecheon Agriculture Technology Center)
Yang, Byoung-Chul (Hanwoo Research Institute)
Publication Information
Journal of Embryo Transfer / v.31, no.3, 2016 , pp. 221-226 More about this Journal
Abstract
The aim of the present study was to assess the embryo development and survivability of post-thawed bovine embryos produced in vitro by addition of cysteine. The rates of metaphase II formation were not differed significantly among three groups(TCM199 73.8%, TCM199 with 0.3% cysteine 76.9%, TCM199 with 0.5% cysteine 83.8%, respectively). No difference of cleavage rate(70.6~74.6%) was seen among three culture medium(TCM199 70.6%, CR1aa 71.3%, SOF 74.6%) with 0.5M cysteine. however, Significantly(P<0.05) higher development rate into blastocyst stage by 0.5M cysteine addition was obtained in SOF medium(35.6%) than in TCM199(27.6%) or CR1aa(26.6%), however no significant differences in the cleavage rates were among three culture medium. After frozen the blastocysts cultured with 0.5M cysteine, The re-expansion rates were 61.3%~86.4% among groups, and hatching rates were 26.3%~46.9% among groups, the rates of re-expansion and hatching were significantly(P<0.05) higher in SOF medium(86.4% and 46.9%) than those in TCM199(61.3% and 26.3%) and CR1aa medium(87.1 and 44.4%). After thawing, the blastocyst re-expansion rate was significantly(P<0.05) higher in in vivo (87.1%) and in vitro (70.3%) embryos. In conclusion, our results demonstrate that supplementation of IVM and IVC medium with 0.5M cysteine improved the quality of in vitro production embryo and post- thawed embryo. Future studies comparing these media systems in well-designed trials should be performed.
Keywords
oocytes; embryo; development; culture; freezing;
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