• Asian-Australasian Journal of Animal Sciences
• /
• v.24 no.2
• /
• pp.181-189
• /
• 2011

Cryopreservation protocols induce partially irreversible damage to mammalian sperm plasma membranes. Previous studies have indicated that adding cholesterol to the plasma membrane, as cholesterol-loaded-cyclodextrins, improves cryosurvival of sperm. Therefore, the purpose of this study was to determine if treating sperm of Markhoz bucks with cholesterol-loaded-cyclodextrins (CLC) (0, 0.75, 1.5, 2.25 and 3 mg/ml diluted $240{\times}10^6$ sperm/ml) in Tris-citric acid-glucose diluents with and without egg yolk (containing 5% glycerol) would improve the post-thaw sperm quality. The motion characteristics were evaluated with a Computer Assisted System Analyzer (CASA); acrosome integrity and vitality were measured with the triple-stain technique. Samples were recovered before and after freezing by means of putting straws into $37^{\circ}C$ water for 30 sec and then parameters were assessed. The results showed that the treatments significantly affected motility, progressive motility, recovery rate, curvilinear velocity, beat cross frequency, live sperm with reacted acrosome, live sperm with unreacted acrosome, dead sperm with reacted acrosorne, and dead sperm with unreacted acrosome during freezing (p<0.05). However; no significant differences were found for average path velocity, straight line velocity, amplitude of lateral head displacement, straightness and linearity (p>0.05). The best results were observed for extender containing 2.25 mg/ml ($240{\times}10^6$ sperm/ml) CLC supplemented with 2.6% egg yolk. In conclusion, the findings of this study indicate improved Markhoz sperm viability and motility following treatment in the presence of egg yolk.

• Composites Research
• /
• v.26 no.1
• /
• pp.21-28
• /
• 2013

The ballistic limit of Carbon/Epoxy composite laminates with the finite effective area are predicted by using the quasi-static perforation test and semi-empirical formula. The perforation energy were calculated from force-displacement curve in quasi-static perforation test. Also, the actual ballistic limit and penetration energy were obtained through the high-velocity impact test. The quasi-static perforation test and high-velocity impact test were conducted for the specimens with 3 different effective areas. In the high-velocity impact test, the air gun impact tester were used, and the ballistic and residual velocity was measured. The required inputs for the semi-empirical formula were determined by the quasi-static perforation tests and high-velocity impact tests. The comparison between semi-empirical formula and high-velocity impact test results were conducted and examined. The ballistic limits predicted by semi-empirical formula were agreed well with high-velocity impact test results.

• Journal of Mechanical Science and Technology
• /
• v.19 no.10
• /
• pp.1919-1931
• /
• 2005

Human joint motion can be kinematically described in three planes, typically the frontal, sagittal, and transverse, and related to experimentally measured data. The selection of reference systems is a prerequisite for accurate kinematic analysis and resulting development of the equations of motion. Moreover, the development of analysis techniques for the minimization of errors, due to skin movement or body deformation, during experiments involving human locomotion is a critically important step, without which accurate results in this type of experiment are an impossibility. The traditional kinematic analysis method is the Angular-based method (ABM), which utilizes the Euler angle or the Bryant angle. However, this analysis method tends to increase cumulative errors due to skin movement. Therefore, the objective of this study was to propose a new kinematic analysis method, Position-based method (PBM), which directly applies position displacement data to represent locomotion. The PBM presented here was designed to minimize cumulative errors via considerations of angle changes and translational motion between markers occurring due to skin movements. In order to verify the efficacy and accuracy of the developed PBM, the mean value of joint dislocation at the knee during one gait cycle and the pattern of three dimensional translation motion of the tibiofemoral joint at the knee, in both flexion and extension, were accessed via ABM and via new method, PBM, with a Local Reference system (LRS) and Segmental Reference system (SRS), and then the data were compared between the two techniques. Our results indicate that the proposed PBM resulted in improved accuracy in terms of motion analysis, as compared to ABM, with the LRS and SRS.

• Computers and Concrete
• /
• v.18 no.6
• /
• pp.1083-1096
• /
• 2016

Beam-column joints are recognized as the weak points of reinforcement concrete frames. The ductility of reinforced concrete (RC) frames during severe earthquakes can be measured through the dissipation of large energy in beam-column joint. Retrofitting and rehabilitating structures through proper methods, such as carbon fiber reinforced polymer (CFRP), are required to prevent casualties that result from the collapse of earthquake-damaged structures. The main challenge of this issue is identifying the effect of CFRP on the occurrence of failure in the joint of a cross section with normal ductility. The present study evaluates the retrofitting method for a normal ductile beam-column joint using CFRP under monotonic and cyclic loads. Thus, the finite element model of a cross section with normal ductility and made of RC is developed, and CFRP is used to retrofit the joints. This study considers three beam-column joints: one with partial CFRP wrapping, one with full CFRP wrapping, and one with normal ductility. The two cases with partial and full CFRP wrapping in the beam-column joints are used to determine the effect of retrofitting with CFRP wrapping sheets on the behavior of the beam-column joint confined by such sheets. All the models are subjected to monotonic and cyclic loading. The final capacity and hysteretic results of the dynamic analysis are investigated. A comparison of the dissipation energy graphs of the three connections shows significant enhancement in the models with partial and full CFRP wrapping. An analysis of the load-displacement curves indicates that the stiffness of the specimens is enhanced by CFRP sheets. However, the models with both partial and full CFRP wrapping exhibited no considerable improvement in terms of energy dissipation and stiffness.

• The Journal of Korean Medicine
• /
• v.17 no.2 s.32
• /
• pp.296-302
• /
• 1996

Pericarpium Citri Nobilis Viride, a traditional herb medicine, has been used in Korea and China for many centuries as a treatment for respiratory disease. The purpose of the present study was to determine the effect of Pericarpium Citri Nobilis Viride on histamine-induced tracheal smooth muscle contraction in rats. Guinea pigs(500g , female) were killed by $CO_2$ exposure and a segment (8-10mm) of the thoracic trachea from each guinea pig was cut into equal segments and mounted 'in pairs' in a tissue bath. Contractile force was measured with force displacement transducers under 0.5g loading tension. The dose of histamine which evoked 50% of maximal response $(ED_{50})$ was obtained from cumulative dose response curves for histamine $(10^{-7}-10^{-4}M)$. Contractions evoked by histamine ($ED_{50}$) were inhibited significantly by Pericarpium Citri Nobilis Viride. The mean percent inhibition was 53.7% (P<0.05) after 1.5mg／ml Pericarpium Citri Nobilis Viride, and 87.7% (P<0.01) after 5.0mg／ml Pericarpium Citri Nobilis Viride. Propranolol $(10^{-7}M)$ slightly but significantly attenuated the inhibitory effects of Pericarpium Citri Nobilis Viride. Following treatment with propranolol the mean present inhibition caused by 1.5 and 5.0mg／ml Pericarpium Citri Nobilis Viride. Indomethacin and methylene blue $(10^{-7}M)$ did not significantly alter the inhibitory effect of Pericarpium Citri Nobilis Viride These results indicate that Pericarpium Citri Nobilis Viride can relax histamine-induced contraction of guinea pig tracheal smooth muscle, and that this inhibition involves in part symphathetic nerve system.

• Journal of Advanced Navigation Technology
• /
• v.18 no.4
• /
• pp.381-386
• /
• 2014

In order to investigate the effect of contact load, which is one of the fretting corrosion factors affecting the electric connector, a coupled fretting corrosion specimens were prepared using a tin-plated brass coupon with a thickness of $3{\mu}m$. Electric resistance of the contact was measured during the fretting corrosion test period. There was increase in resistance with fretting cycles. The change in resistance can be classified by 3 stages. The first stage exhibited low and stable resistance. Second stage showed steady increment of the resistance and third stage showed very high and intermittent resistance. The relationship between the failure cycle (Nf) and contact force (P) can be drawn as; It is possible to draw the prediction equation for the failure cycle of the electric connector corresponding to the very high and intermittent resistance under various environment conditions through the fretting tests under various conditions such as load, displacement, temperature.

• Journal of Korean Neurosurgical Society
• /
• v.46 no.2
• /
• pp.144-151
• /
• 2009

Objective: To compare two testing protocols for evaluating range of motion (ROM) changes in the preloaded cadaveric spines implanted with a mobile core type Charite$^{TM}$ lumbar artificial disc. Methods: Using five human cadaveric lumbosacral spines (L2-S2), baseline ROMs were measured with a bending moment of 8 Nm for all motion modes (flexion/extension, lateral bending, and axial rotation) in intact spine. The ROM was tracked using a video-based motion-capturing system. After the Charite$^{TM}$ disc was implanted at the L4-L5 level, the measurement was repeated using two different methods: 1) loading up to 8 Nm with the compressive follower preload as in testing the intact spine (Load control protocol), 2) loading in displacement control until the total ROM of L2-S2 matches that when the intact spine was loaded under load control (Hybrid protocol). The comparison between the data of each protocol was performed. Results: The ROMs of the L4-L5 arthroplasty level were increased in all test modalities (p < 0.05 in bending and rotation) under both load and hybrid protocols. At the adjacent segments, the ROMs were increased in all modes except flexion under load control protocol. Under hybrid protocol, the adjacent segments demonstrated decreased ROMs in all modalities except extension at the inferior segment. Statistical significance between load and hybrid protocols was observed during bending and rotation at the operative and adjacent levels (p< 0.05). Conclusion: In hybrid protocol, the Charite$^{TM}$ disc provided a relatively better restoration of ROM, than in the load control protocol, reproducing clinical observations in terms of motion following surgery.

• Journal of Oral Medicine and Pain
• /
• v.17 no.2
• /
• pp.63-73
• /
• 1992

This study was performed for the purpose of investigating the relation between width of temporomandibular joint space and craniofacial morphology in patients with CMDs. The subjects utilized here were aged from 16 to 63 years old and 19 male and 46 female. For the study, each patient was taken radiographs by Transcranial and Lateral Cephalographic projection and the films were traced with routine method. The measured items were anterior, superior, posterior joint space and relative condylar position to the deepest part of glenoid fossa in Transcranial film and items related vertical and/or horizontal growth in Cephalogram. The data were processed with SPSS/PC+ package for statistical analysis. The obtained results were as follows : 1. Anterior joint space in affected side were wider than that of unaffected side, but for superior and posterior joint space, the value of unaffected side were more than those of affected side. 2. Superior or posterior joint space of affected side were significantly correlated with items related vertical growth, such as ramus height, Jarabak ratio, FMA. From this, the following cold be proposed, the wider the joint space of superior or posterior aspect of affected side was, the more the patient showed growth pattern of counter clockwise. 3. Superior or posterior joint space of affected side were significantly correlated with items related horizontal growth, such as SNPo, NAPo, APDI and ANB. From this the following could be proposed, the wider the joint space of superior or posterior aspect of affected side was, the more the patient showed anterior displacement of mandible. 4. It would be proposed that the diagnositic value of superior and posterior joint space of affected side in transcranial radiograph was excellent.

• Journal of the Korea institute for structural maintenance and inspection
• /
• v.17 no.1
• /
• pp.46-54
• /
• 2013

Two different UHPCCs having different fiber lengths and volume fractions are considered to be applied to bridge decks. The objective of this study is to estimate cracking resistance of the two UHPCCs. The notched beam tests were performed with the UHPCCs, and the relationships between load and CMOD(Crack Mouth Opening Displacement) were obtained from the tests. The tensile stress and crack opening relationships optimally fitting the measured load-CMOD curves were found through the inverse analyses. The UHPCC with 2% volume fraction of 13 mm long fiber has lower fracture energy than the UHPCC with 0.5% and 1.0% volume fractions of 16.3 mm and 19.5 mm long fibers, respectively. It indicates that the latter UHPCC is more effective in uniformly distributing crack formation and reducing crack width.

• Composites Research
• /
• v.16 no.3
• /
• pp.9-17
• /
• 2003

In order to simulate the crack connection between cords and the interply crack growth in the belt-layer of real tire, 2 ply rubber/cord laminate specimens with exposed edges were tested in 4~11mm displacement control. Measurement of the crack connection is evaluated when crack reaches the half of the length between 45$^{\circ}$ aligned cords, and the amount of the crack growth is measured by the steel probe method. 2 dimensional analytic modeling was performed to simulate the crack connection between cords at the exposed edges. Also, the theoretical life of the specimens was calculated from the crack connection life between cords(critical value) and from the critical value to the final failure by the use of Tearing energy(T); the strain energy release per unit area of one fracture surface of a crack. Then, theoretical life was compared with those of experiments. The life prediction up to the critical value has about 20% error compared to experimental life, and up to the final failure about 65% error. Therefore, total theoretical life has about 45% error compared to the experimental life, which is conceivable in the case of rubber.