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Diversity and Plant Growth Promoting Capacity of Endophytic Fungi Associated with Halophytic Plants from the West Coast of Korea

  • Khalmuratova, Irina;Kim, Hyun;Nam, Yoon-Jong;Oh, Yoosun;Jeong, Min-Ji;Choi, Hye-Rim;You, Young-Hyun;Choo, Yeon-Sik;Lee, In-Jung;Shin, Jae-Ho;Yoon, Hyeokjun;Kim, Jong-Guk
    • Mycobiology
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    • v.43 no.4
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    • pp.373-383
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    • 2015
  • Five halophytic plant species, Suaeda maritima, Limonium tetragonum, Suaeda australis, Phragmites australis, and Suaeda glauca Bunge, which are native to the Muan salt marsh of South Korea, were examined for fungal endophytes by sequencing the internal transcribed spacer (ITS) region containing ITS1, 5.8S rRNA, and ITS2. In total, 160 endophytic fungal strains were isolated and identified from the roots of the 5 plant species. Taxonomically, all 160 strains belonged to the phyla Ascomycota, Basidiomycota, and Zygomycota. The most dominant genus was Fusarium, followed by the genera Penicillium and Alternaria. Subsequently, using 5 statistical methods, the diversity indices of the endophytes were determined at genus level. Among these halophytic plants, P. australis was found to host the greatest diversity of endophytic fungi. Culture filtrates of endophytic fungi were treated to Waito-C rice seedlings for plant growth-promoting effects. The fungal strain Su-3-4-3 isolated from S. glauca Bunge provide the maximum plant length (20.1 cm) in comparison with wild-type Gibberella fujikuroi (19.6 cm). Consequently, chromatographic analysis of the culture filtrate of Su-3-4-3 showed the presence of physiologically active gibberellins, $GA_1$ (0.465 ng/mL), $GA_3$ (1.808 ng/mL) along with other physiologically inactive $GA_9$ (0.054 ng/mL) and $GA_{24}$ (0.044 ng/mL). The fungal isolate Su-3-4-3 was identified as Talaromyces pinophilus.

Corrosion of Calcareous Rocks and Ground Subsidence in the Muan Area, Jeonnam, Korea (전남 무안지역에 분포하는 석회질암의 용식작용과 지반침하)

  • Ahn, Kun-Sang
    • The Journal of the Petrological Society of Korea
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    • v.16 no.2 s.48
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    • pp.47-58
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    • 2007
  • This study examines the distribution of basement rocks in Gyochon-ri, Muan-eup, Muan-gun, Jeonnam where ground subsidence occurred in June 2005, and traces corrosion of limestone. Mica schist and rhyolite are distributed in the surface of the study area, but thick limestone layer with large and small caverns are distributed underground. A horizon of limestone with maximum width of 300 m and 4 km of length was found along the detour which is in the north of pound subsidence. Such identification of limestone presence would be very useful to predict potential ground subsidence. Limestone in this area was disturbed by fold and fault due to severe shearing deformation. Small caverns were frequently found in anticline part of folds formed in limestone layer. Schists with different thicknesses were intercalated in the limestone with shearing deformation and consist of sheet silicate minerals (chlorite and mica) and quartz. In sections of weathered specimen, it is shown that biotite of schist part was altered into chlorite and corrosion of calcite around the schist followed. This suggest that ground water permeated between intercalated sheet silicate minerals and corrosion of limestone began. And small caverns were generated where active corrosion occurred. This study suggests that because of many reasons (for instance, reclamation of the Bulmu reservior and excess pumping), cavern water level was lowered and cave sediments were removed, and it caused ground subsidence to occur.

Studies on the Natural Mortality of the Young Short Necked Clam, Tapes Japonica-II . Resistance to Temperature , Salinity and Exposure for the Young Short Necked Clam (바지락치패의 폐사에 관한 연구-II 바지락 치패의 온도, 염분, 노출에 대한 저항성)

  • CHOE, Sang
    • The Korean Journal of Zoology
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    • v.9 no.1
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    • pp.7-15
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    • 1966
  • In a previous paper, the dirunal and monthly changes of the tidal flat temperature and chlorinity were observed. In this paper the resistance of the young short necked clam in various growth stages to the temperature, to the chlorinity and to the exposure were studies. The young clam used were in early (E), medium (M) and late (L) young stages whose shell lengths were 2.0-3.5mm, 9.0-11.0mm. and 14.0-16.0mm., respectively. The results were : 1. At various sea water temperatures , the chlorinity resistance of the young clam was directly proportional to the shell length. 2. When both inadequate sea water temperature and abnormal chlorinity are simultaneously applied, the resistance of these young clams was more markedly reduced than the case of applying either one of these conditions. 3. In clams of M and L, no lethal effect were observed when daily immersion of four to eight hours for a week into the sea water of any concentration of chloriity at 26-34$^{\circ}C$, whereas in E, 37-90% of mortalities were obtained by immersion of eight hours daily into both the fresh water and the sea water of higher chloriniites (more than 23.5$\textperthousand$ Cl) at the same temperature above. 4. The lower critical thermal maximum for lethal to the young clam was 38$^{\circ}C$. With four hours immersion daily at the water temperature of 38$^{\circ}C$, the mortalities of E, M and L to the lower chlorinities (less than 6.7$\textperthousand$Cl) were 100, 70-100 and 27-37% respectively ; to the higher chlorinities (more than 23.5$\textperthousand$Cl) 10-70, 10-37 and 3 % respectively ; to the normal range of chlorinities (13.4-16.8$\textperthousand$Cl) 0-13, 3 and 0 % respectively. 5. No lethal effects were observed in E and M clams immersed continuously for seven days in sea water with chlornities of 7.2 -21.7$\textperthousand$Cl at 18-24$^{\circ}C$, while notable mortalities were observed in E which had been kept at lower (less than 4.8$\textperthousand$ Cl) and higher (more than 24.1$\textperthousand$ Cl) chlorinites. 6. Although the resistance of the young clam to the chlorinity may have to be related closely to the life history of the clam prior subject to the experiment, the adapted chlorinity range was 7.2-19.3$\textperthousand$ Cl and the optimum range was 13.4-16.8$\textperthousand$Cl. 7. Remarkable lethal effects were observed for the E and M clams to the exposure temperature of 38$^{\circ}C$ whereas the L and had no such fatal results.

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Needle Characteristics and Genetic Variation of Pinus pumila Natural Population in Mt. Seorak (설악산 눈잣나무 천연집단의 침엽특성과 유전변이)

  • Song, Jeong-Ho;Lim, Hyo-In;Jang, Kyung-Hwan
    • Korean Journal of Plant Resources
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    • v.25 no.5
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    • pp.517-522
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    • 2012
  • This study was conducted to investigate the morphological and anatomical characteristics of needle and the genetic diversity of Pinus pumila Regel which is a unique and the southern peripheral population in South Korea. ANOVA test showed that there were significant differences among individuals within population in all 8 needle characteristics. Average characteristics of 66 individuals were 53.59 mm in needle length, 0.78 mm in needle width, 68.98 in needle index, 0.65 mm in needle thickness, 4.56 ea. in maximum stomata row, 3.80 ea. in minimum stomata row, 8.36 ea. in total stomata row and 1.71 ea. in resin canals, respectively. Resin canal per needle of this species ranged from one to three, depending on external type. Especially, arrangement types were 69.47% in two resin canals and 30.45% in a single resin canal. A total of 78 bands was generated from 9 selected I-SSR primers. The estimates of genetic variation were 61.5% in proportion of polymorphic bands (P), 1.698 in effective number of alleles ($A_e$), 0.388 in expected heterozygosity ($H_e$) and 0.567 in Shannon's information index (S.I.), respectively.

Phylogenetic Study of Korean Chrysosplenium Based on nrDNA ITS Sequences (ITS 염기서열에 의한 한국산 괭이눈속(Chrysosplenium)의 계통학적 연구)

  • Han, Jong-Won;Yang, Sun-Gyu;Kim, Hyun-Jun;Jang, Chang-Gee;Park, Jeong-Mi;Kang, Shin-Ho
    • Korean Journal of Plant Resources
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    • v.24 no.4
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    • pp.358-369
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    • 2011
  • The internal transcribed spacer (ITS) regions of nuclear ribosomal DNA from genus Chrysosplenium were sequenced to address phylogenetic relationship. ITS including 5.8S sequence varied in length from 647 bp to 653 bp. Among them, 219 sites were variable sites with parsimony-informative. The aligned sequences were analyzed by maximum parsimony (MP) and neighbor-joining (NJ) methods. In the strict consensus trees of parsimony analysis, the monophyly of Chrysosplenium was supported by 100% bootstrap value. The first clade, C. pseudofauriei was at the basal position of the genus, and others formed two clades with high bootstrap support. The second clade included Ser. Pilosa and Ser. Oppositifolia and third clade included Ser. Alternifolia and Ser. Flagellifera. The NJ trees showed essentially the same topology. Finally, DNA sequences of ITS regions were useful phylogenetic marker in this genus. Based on the ITS and ridge seed morphological results, C. sphaerospermum Maxim. and C. valdepilosum (Ohwi) S.H. Kang & J.W. Han were discussed their scientific names and taxonomic positions.

Interaction of Brassinolide with Other Known Plant Growth Regulators (Brassinolide와 기존 식물생장조절제(植物生長調節劑)와의 상호작용(相互作用))

  • Choi, C.D.;Takematsu, T.;Takeuchi, Y.;Kim, K.U.
    • Korean Journal of Weed Science
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    • v.7 no.1
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    • pp.78-83
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    • 1987
  • This study was attempted to evaluate the combining effect of HBR (homobrassinolide) with the known growth regulators such as GA (gibberellic acid), BA(6-benzyl aminopurine), IAA (indole-3-acetic acid), B-9 (N-dimethylamino succinamic acid) and CCC (2-chloroethyl-trimethylammonium chloride) on the growth of radish hypocotyl. A single application of HBR increased hypocotyl growth as its rates increased from 0.1 to 1.0 ppm, showing a maximum increase at 1.0 ppm. GA and BA had no direct effects on hyopcotyl growth, but IAA showed some effect as its concentration increased. However, the mixed application of HBR with GA, BA and IAA increased the length of radish hypocotyl as the concentration of HBR became higher. The mixture of HBR with GA and BA showed antagonistic reaction on radish hypocotyl growth, but synergistic effect was shown in the higher rate mixture of HBR with IAA in the range of HBR at 0.03 to 0.30 ppm with IAA at 3.0 to 10.0 ppm, but antagonistic or additive response at the mixture of low rates. An increased growth of hypocotyl by HBR was ified by CCC, showing the strong antagonistic reaction, but B-9 was not able to ify HBR's effect on hypocotyl growth.

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Duration of Competition and the Competitive Ability of Red Rice with Rich by Replacement Diagram (적미(赤米)의 경합기간(競合期間)과 밀도(密度)가 수도생육(水稻生育) 및 수량(收量)에 미치는 영향(影響))

  • Ree, D.W.;Kim, J.C.;Kim, Y.H.;Hong, Y.K.;Son, S.Y.
    • Korean Journal of Weed Science
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    • v.7 no.1
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    • pp.45-51
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    • 1987
  • This experiment was conducted to find out the critical competitive period and competitive ability of rice against red rice. Plant height and number of tillers of rice decreased as red rice competed with rice during active tillering stage. The maximum tillering stage of rice competed with red rice became about 10days earlier than those non-competed. Significant yield reduction of rice was observed in the plots competed for 20 days after transplating or longer. Such a yield reduction can be mainly attributed to the decrease in number of panicles per hill and grain number per panicle. In a replacement diagram for rice and red rice, their competition is turned out being antagonized. The relative yield total was considered lower than 1. Grain number, culm and panicle length of rice was not affected significantly by inter-specific competition. Rice grain yield reduction was highly correlated with the number of panicles of red rice. Three hundred panicles/$m^2$ of the red rice were necessary to reduce rice grain yield by 50% of the check plot.

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Design and Implementation of Dual Wideband Dipole Type Antenna for the Reception of S-DMB and 2.4/5 GHz WLAN Signals (S-DMB와 2.4/5 GHz WLAN 신호 수신을 위한 이중 광대역 다이폴형 안테나의 설계 및 구현)

  • Kim, Sung-Min;Yang, Woon-Geun
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.17 no.11 s.114
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    • pp.1021-1029
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    • 2006
  • In this paper, we designed and implemented a dual wideband dipole type antenna for the reception of S-DMB (Satellite Digital Multimedia Broadcasting) and 2.4/5 GHz WLAN(Wireless Local Area Network) signals. The proposed antenna based on conventional monopole type dual band antenna was implemented as planar wideband dipole type antenna with the volume of $8{\times}33.8{\times}1.68mm^3$. The proposed antenna is printed type on FR4 substrate of 1.6 mm thick and composed of a dipole type antenna for low frequency band and two symmetric structured resonance elements for high frequency band. We confirmed antenna area with dense surface current for each frequency band with simulation. By varying the length of the antenna area with dense surface current, we could vary resonance frequency of each frequency band separately. Impedance bandwidths$(VSWR{\leq}2)$ are 362 MHz(14.23 %) for 2 GHz band and 1188 MHz(22.13, %) for 5 GHz band which show wideband characteristic. Measured maximum gains were 4.33 dBi for 2 GHz band and 5.48 dBi for 5 GHz band which showed improved performance. And the implemented antenna has a good omni-directional radiation pattern characteristic.

Tunneling in Severe Groundwater Inflow Condition (지하수 과다유입 조건하에서의 터널굴착)

  • Lee, Young-Nam;Kim, Dae-Young
    • Journal of the Korean GEO-environmental Society
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    • v.7 no.2
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    • pp.67-76
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    • 2006
  • For a hydro power plant project, the headrace tunnel having a finished diameter of 3.3 m was constructed in volcanic rocks with well-developed vertical joint and high groundwater table. The intake facility was located 20.3km upstream of the powerhouse and headrace tunnel of 20km in length and penstock of 440m in height connected the intake and the powerhouse. The typical caldera lake, Lake Toba set the geology at the site the caving of the ground caused tension cracks in the vertical direction to be developed and initial stresses at the ground to be released. High groundwater table(the maximum head of 20bar) in the area of well-connected vertical joints delayed the progress of tunnel excavation severely due to the excessive inflow of groundwater. The excavation of tunnel was made using open-shield type TBM and mucking cars on the rail. High volume of water inflowraised the water level inside tunnel to 70cm, 17% of tunnel diameter (3.9m) and hindered the mucking of spoil under water. To improve the productivity, several adjustments such as modification of TBM and mucking cars and increase in the number of submersible pumps were made forthe excavation of severe water inflow zone. Since the ground condition encountered during excavation turned out to be much worse, it was decided to adopt PC segment lining instead of RC lining. Besides, depending on the conditions of the water inflow, rock mass condition and internal water pressure, one of the invert PC segment lining with in-situ RC lining, RC lining and steel lining was applied to meet the site specific condition. With the adoption of PC segment lining, modification of TBM and other improvement, the excavation of the tunnel under severe groundwater condition was successfully completed.

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EFFECT OF SOLDERING CONDITION ON THE TENSILE STRENGTH OF TITANIUM SOLDER JOINT WITH 14K GOLD SOLDER (납착조건이 14K 금납을 이용한 티타늄 납착부의 인장강도에 미치는 영향)

  • Choi, Jeoung-Ho;Kim, Tae-Jo;Song, Kwang-Yeob;Park, Charn-Woon
    • The Journal of Korean Academy of Prosthodontics
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    • v.35 no.3
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    • pp.566-576
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    • 1997
  • This study was performed to evaluate the tensile strength of solder joint in titanium and the wettability of 14K gold solder on titanium. Two pieces of titanium rod 30 mm in length and 3mm in diameter were butt-soldered with a 14K gold solder using the electric resistance heating under flux-argon atmosphere, the infrared heating under argon atmosphere, and the infrared heating under vacuum-argon atmosphere. A tensile test was performed at a crosshead speed of 0.5 mm/min, and fracture surfaces were examined by SEM. To evaluate the wettability of 14K gold solder on titanium, titanum plates of a $17{\times}17{\times}1mm$ were polished with #80-#2000 emery papers, and the spreading areas of solder 10 mg were measured by heating at 840 * for 60 seconds. The solder-matrix interface regions were etched by the solution of 10% KCN-10% (NH4)2S2O8, and analyzed by EPMA. The results obtained were summarized as follows ; 1. The maximum tensile strength was obtained when the titanium surface was polished with #2000 emery paper and soldered using the electric resistance heating under flux-argon atmosphere. Soldering strengths showed the significant difference between the electric resistance heating and the infrared heating(p<0.05). 3. The fracture surfaces showed the aspect of brittle fracture, and the failure developed along the interfaces of solder-matrix reaction zone. 4. The EPMA data for the solder-matrix interface region revealed that the diffusion of Au and Cu occurred to the titanium matrix, and the reaction zone showed the higher contents of Au, Cu and Ti than others.

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