• IMMUNE NETWORK
• /
• v.5 no.3
• /
• pp.144-149
• /
• 2005

Background: Fc receptor-mediated phagocytosis is a complex process involving the activation of kinases and phosphatases. FcgammaRIIB has been known to transduces inhibitory signals through an immunoreceptor tyrosine-based inhibitory motif (ITIM) in cytoplasmic domains. In this study, we examined the involvement of inositol-phosphatase in the Fc receptor-mediated phagocytosis. Methods: J774 cells were infected using vaccinia viral vector containing SH2 domain-containing inositol-phosphatase (SHIP) cDNA and stimulated with the sensitized sheep red blood cells. Results: Stimulation of J774 cells induced the tyrosine phosphorylation of SHIP which was maximal at 5 minutes. Phosphatidylinositol-3 (PI-3) kinase inhibitor (wortmannin) inhibits J774 cell phagocytosis of sensitized sheep red blood cells in a dose-dependent manner. Heterologious expression of SHIP in J774 cells inhibits phagocytosis of sensitized sheep red blood cells in a dose-dependency manner, but catalytically dead mutants of SHIP has no effect on phagocytosis. Conclusion: These results strongly suggest that the active signals mediated by PI-3 kinase are opposed by inhibitory signals through SHIP in the regulation of Fc receptor-mediated phagocytosis.

• Journal of applied mathematics & informatics
• /
• v.7 no.1
• /
• pp.1-28
• /
• 2000

In the present paper, we introduce fundamental results in the KKM theory for G-convex spaces which are equivalent to the Brouwer theorem, the Sperner lemma, and the KKM theorem. Those results are all abstract versions of known corresponding ones for convex subsets of topological vector spaces. Some earlier applications of those results are indicated. Finally, We give a new proof of the Himmelberg fixed point theorem and G-convex space versions of the von Neumann type minimax theorem and the Nash equilibrium theorem as typical examples of applications of our theory.

• Journal of the Korean Mathematical Society
• /
• v.40 no.1
• /
• pp.1-28
• /
• 2003

The Iwahori-Hecke algebra $H_{k}$ ( $q^2$) of type A acts on the k-fold tensor product space of the natural representation of the quantum superalgebra (equation omitted)$_{q}$(gl(m, n)). We show the Hecke algebra $H_{k}$ ( $q^2$) and the quantum superalgebra (equation omitted)$_{q}$(gl(m n)) have commuting actions on the tensor product space, and determine the centralizer of each other. Using this result together with Gyoja's q-analogue of the Young symmetrizers, we construct highest weight vectors of irreducible summands of the tensor product space.

• Journal of the Korean Mathematical Society
• /
• v.50 no.6
• /
• pp.1311-1332
• /
• 2013

This paper concerns the evolution of a closed hypersurface of the hyperbolic space, convex by horospheres, in direction of its inner unit normal vector, where the speed equals a positive power ${\beta}$ of the positive mean curvature. It is shown that the flow exists on a finite maximal interval, convexity by horospheres is preserved and the hypersurfaces shrink down to a single point as the final time is approached.

• Journal of Veterinary Clinics
• /
• v.4 no.2
• /
• pp.505-514
• /
• 1987

Babesia gibsoni infection was diagnosed and treated in three hunting dogs which were hospitalized to the Veterinary Teaching Hospital, College of Veterinary Medicine, Seoul National University between April 4 and August 29, 1987. All three dogs revealed severe anemia, hemoglobinuria, splenomegaly and markedly decreased PCV, RBC count and hemoglobin. The anemia was regenerative, as characterized by increased numbers of nucleated erythrocytes, polychromasia, anisocytosis, reticulocytosis. B. gibsoni was identified by examination of blood smear stained with Giemsa stain. The forms of B. gibsoni identified in this report were pleomorphic such as singnet ring, oval, comma, dot and elongated forms. The maximal percentages of erythrocytes infected with one or more B. gibsoni organisms were 39%, 20% and 40%, respectively. The Tick, Haemophysalis longicornis was assumed to be the vector of babesiosis in these cases. Specific treatment consisted of diminazene aceturate and supportive treatment consisted of whole blood transfusion, lactated Ringer's solution, vitamin B complex and broad spectrum antibiotics. All three dogs had convalesced successfully after treatment.

• Proceedings of the KIEE Conference
• /
• 2007.07a
• /
• pp.187-189
• /
• 2007

In this paper, the cascade 3-phase IHMCL inverter using two low frequency transformers is proposed. The proposed inverter is constructed by connecting a 3-phase IHCML inverter. the cascade 3-phase IHCML inverter has several advantages. One advantage is that only one input power source is required because of using transformers to isolate. Another advantage is that the switching frequency of the high power switches is almost fundamental frequency of reference and the other the switching frequency of the low power switches is higher. It can be known that cascade 3 phase IHCML inverter has the excellent efficiency and the outstanding electric quality. lastly, we tested the 5kW cascade 3-phase IHCML inverter to clarify the proposed electric circuit and reasonableness of control signal for the proposed inverter.

• Journal of the Korean Mathematical Society
• /
• v.59 no.5
• /
• pp.927-944
• /
• 2022

Let $\vec{p}{\in}(0,\;1]^n$ be an n-dimensional vector and A a dilation. Let $H^{\vec{p}}_A(\mathbb{R}^n)$ denote the anisotropic mixed-norm Hardy space defined via the radial maximal function. Using the known atomic characterization of $H^{\vec{p}}_A(\mathbb{R}^n)$ and establishing a uniform estimate for corresponding atoms, the authors prove that the Fourier transform of $f{\in}H^{\vec{p}}_A(\mathbb{R}^n)$ coincides with a continuous function F on ℝⁿ in the sense of tempered distributions. Moreover, the function F can be controlled pointwisely by the product of the Hardy space norm of f and a step function with respect to the transpose matrix of A. As applications, the authors obtain a higher order of convergence for the function F at the origin, and an analogue of Hardy-Littlewood inequalities in the present setting of $H^{\vec{p}}_A(\mathbb{R}^n)$.

• Journal of Microbiology and Biotechnology
• /
• v.18 no.6
• /
• pp.1136-1140
• /
• 2008

The Rhodopseudomonas palustris KUGB306 hemA gene codes for 5-aminolevulinic acid (ALA) synthase. This enzyme catalyzes the condensation of glycine and succinyl-CoA to yield ALA in the presence of the cofactor pyridoxal 5'-phosphate. The R. palustris KUGB306 hemA gene in the pGEX-KG vector system was transformed into Escherichia coli BL21. The effects of physiological factors on the extracellular production of ALA by the recombinant E. coli were studied. Terrific Broth (TB) medium resulted in significantly higher cell growth and ALA production than did Luria-Bertani (LB) medium. ALA production was significantly enhanced by the addition of succinate together with glycine in the medium. Maximal ALA production (2.5 g/l) was observed upon the addition of D-glucose as an ALA dehydratase inhibitor in the late-log culture phase. Based on the results obtained from the shake-flask cultures, fermentation was carried out using the recombinant E. coli in TB medium, with the initial addition of 90 mM glycine and 120 mM succinate, and the addition of 45 mM D-glucose in the late-log phase. The extracellular production of ALA was also influenced by the pH of the culture broth. We maintained a pH of 6.5 in the fermenter throughout the culture process, achieving the maximal levels of extracellular ALA production (5.15 g/l, 39.3 mM).

• Restorative Dentistry and Endodontics
• /
• v.19 no.1
• /
• pp.231-254
• /
• 1994

Fracture of cusp, on posterior teeth, especially those carious or restored, is major cause of tooth loss. Inappropriate treatments, such as unnecessarily wide cavity preparations, increase the potential of further trauma and possible fracture of the remaining tooth structures. Fracture potential may be directly related to the stresses exerted upon the tooth during masticatory function. The purpose of this study is to evaluate the fracture resistance of tooth, restored with composite resin inlay. In this study, MOD inlay cavity prepared on maxillary first premolar and restored with composite resin inlay. Three dimensional finite element models with eight nodes isoparametric solid element, developed by serial grinding-photographing technique. These models have various occlusal isthmus and depth of cavity, 1/2, 1/3 and 1/4 of isthmus width and 0.7, 0.85 and 1.0 of depth of cavity. The magnitude of load was 474 N and 172 N as presented to maximal biting force and normal chewing force. These loads applied onto ridges of buccal and lingual cusp. These models analyzed with three dimensional finite element method. The results of this study were as follows : 1. There is no difference of displacement between width of occlusal isthmus and depth of cavity. 2. The stress concentrated at bucco-mesial comer, bucco-disal comer, pulpal line angle and the interface area between internal slopes of cusp and resin inlay. 3. The vector of stress direct to buccal and lingual side from center of cavity, to tooth surface going on to enamel. The magnitude of vector increase from occlusal surface to cervix. 4. The crack of tooth start interface area, between internal slop of buccal cusp and resin inlay. It progresses through buccopulpal line angle to cervix at buccomesial and buccodistal comer. 5. The influence with depth of cavity to fracture of tooth was more than width of isthmus. 6. It would be favorable to make the isthmus width narrower than a third of the intercuspal distance and depth of cavity is below 1 : 0.7.

• 한국생물공학회:학술대회논문집
• /
• 2005.04a
• /
• pp.420-422
• /
• 2005

TMC (Tautomycetin) is a liner polyketide immunosuppressive antifungal compound produced by Streptomyces spp. Inhibition of T cell proliferation with TMC was observed highly efficient at 100-fold lower than those needed to achieve maximal inhibition with cyclosporin A. To elucidate the biosynthetic pathway of TMC, a genomic DNA library was constructed using a E. coil-Streptomyces shuttle cosmid vector, pOJ446. The DNA libraries were screened by colony blot hybridization using several polyketide ${\beta}-ketosynthase$ (KS) probes amplified from TMC-producing Streptomyces genomic DNA using polymerase chain reaction (PCR), of which the degenerate primers were designed based on the highly conserved sequences present in KS domains of various type I polyketide synthase genes in Streptomyces species. This library construction and screening approach led to the isolation of several positive cosmid clones representing type I polyketide biosynthetic gene clusters. In addition, a Streptomyces regulatory gene called afsR2 (a global regulatory gene stimulating antibiotic production in both S. coelicolor and S. lividans) was successfully integrated into the TMC-producing Streptomyces chromosome via E. coil-Streptomyces heterologous conjugation mehtod. The more detailed results of production improvement and genetic characterization of TMC-producing Streptomyces spp. will be discussed.