• Safety and Health at Work
• /
• 제13권1호
• /
• pp.99-103
• /
• 2022

Background: Firefighters may experience high environmental temperatures or carry out intensive physical tasks, or both, which leads to increased core body temperature and risk of fatalities. Hence there is a need to remotely and non-invasively monitor core body temperature. Methods: Estimated (heart rate algorithm) and actual core body temperature (ingested telemetric pill) measures were collected simultaneously for comparison during training exercises on 44 firefighter volunteers. Results: Prediction of core body temperature varied, with no specific identifiable pattern between the algorithm values and directly measured body core temperatures. Group agreement of Lin's Concordance of 0.74 (95% Upper 0.75, lower CI 0.73), was deemed poor. Conclusion: From individual agreement data Lin's Concordance was variable (Min 0.11, CI 0.13-0.01; Max 0.83, CI 0.86-0.80), indicating that the heart rate algorithm approach was not suitable for core body temperature monitoring in this population group, especially at the higher more critical core body temperatures seen.

• Journal of Ginseng Research
• /
• 제37권3호
• /
• pp.283-292
• /
• 2013

Ginsenosides are divided into two groups based on the types of the panaxadiol group (e.g., ginsenoside-Rb1 and -Rc) and the panaxatriol group (e.g., ginsenoside-Rg1 and -Re). Among them, ginsenoside-Re (G-Re) is one of the compounds with the highest content in Panax ginseng and is responsible for pharmacological effects. However, it is not yet well reported if G-Re increases the hemodynamics functions on ischemia (30 min)/reperfusion (120 min) (I/R) induction. Therefore, in the present study, we investigated whether treatment of G-Re facilitated the recovery of hemodynamic parameters (heart rate, perfusion pressure, aortic flow, coronary flow, and cardiac output) and left ventricular developed pressure (${\pm}dp/dt_{max}$). This research is designed to study the effects of G-Re by studying electrocardiographic changes such as QRS interval, QT interval and R-R interval, and inflammatory marker such as tissue necrosis factor-${\alpha}$ (TNF-${\alpha}$) in heart tissue in I/R-induced heart. From the results, I/R induction gave a significant increase in QRS interval, QT interval and R-R interval, but showed decrease in all hemodynamic parameters. I/R induction resulted in increased TNF-${\alpha}$ level. Treatment of G-Re at 30 and $100{\mu}M$ doses before I/R induction significantly prevented the decrease in hemodynamic parameters, ameliorated the electrocardiographic abnormality, and inhibited TNF-${\alpha}$ level. In this study, G-Re at $100{\mu}M$ dose exerted more beneficial effects on cardiac function and preservation of myocardium in I/R injury than $30{\mu}M$. Collectively, these results indicate that G-Re has distinct cardioprotectective effects in I/R induced rat heart.

• 한국지진공학회논문집
• /
• 제19권2호
• /
• pp.63-74
• /
• 2015

This study investigated the influence of probabilistic variability in stiffness and nonlinearity of soil on response of nuclear power plant (NPP) structure subjected to seismic loads considering the soil-structure interaction (SSI). Both deterministic and probabilistic methods have been employed to evaluate the dynamic responses of the structure. For the deterministic method, $SRP_{min}$ method given in USNRC SRP 3.7.2(2013) (envelope of responses using three shear modulus profiles of lower bound($G_{LB}$), best estimate($G_{BE}$) and upper bound($G_{UB}$)) and $SRP_{max}$ method (envelope of responses by more than three ground profiles within range of $G_{LB}{\leq}G{\leq}G_{UB}$) have been considered. The probabilistic method uses the Latin Hypercube Sampling (LHS) that can capture probabilistic feature of soil stiffness defined by the median and the standard deviation. These analysis results indicated that 1) number of samples shall be larger than 60 to apply the probabilistic approach in SSI analysis and 2) in-structure response spectra using equivalent linear soil profiles considering the nonlinear behavior of soil medium can be larger than those based on low-strain soil profiles.

• Journal of Microbiology and Biotechnology
• /
• 제17권6호
• /
• pp.905-912
• /
• 2007

A novel ${\beta}-glucosidase$ gene, bglA, was isolated from uncultured soil bacteria and characterized. Using genomic libraries constructed from soil DNA, a gene encoding a protein that hydrolyzes a fluorogenic analog of cellulose, 4-methylumbelliferyl ${\beta}-D-cellobioside$ (MUC), was isolated using a microtiter plate assay. The gene, bglA, was sequenced using a shotgun approach, and expressed in E. coli. The deduced 55-kDa amino acid sequence for bglA showed a 56% identity with the family 1 glycosyl hydrolase Chloroflexus aurantiacus. BglA included two conserved family 1 glycosyl hydrolase regions. When using $p-nitrophenyl-{\beta}-D-glucoside$ (pNPG) as the substrate, the maximum activity of the purified ${\beta}-glucosidase$ exhibited at pH 6.5 and $55^{\circ}C$, and was enhanced in the presence of $Mn^{2+}$. The $K_m\;and\;V_{max}$ values for the purified enzyme with pNPG were 0.16 mM and $19.10{\mu}mol/min$, respectively. The purified BglA enzyme hydrolyzed both pNPG and $p-nitrophenyl-{\beta}-D-fucoside$. The enzyme also exhibited substantial glycosyl hydrolase activities with natural glycosyl substrates, such as sophorose, cellobiose, cellotriose, cellotetraose, and cellopentaose, yet low hydrolytic activities with gentiobiose, salicin, and arbutin. Moreover, BglA was able to convert the major ginsenoside $Rb_1$ into the pharmaceutically active minor ginsenoside Rd within 24 h.

• Journal of Microbiology and Biotechnology
• /
• 제28권4호
• /
• pp.638-644
• /
• 2018

In this study, the immobilization of xylanase using a protein-inorganic hybrid nanoflower system was assessed to improve the enzyme properties. The synthesis of hybrid xylanase nanoflowers was very effective at $4^{\circ}C$ for 72 h, using 0.25 mg/ml protein, and efficient immobilization of xylanase was observed, with a maximum encapsulation yield and relative activity of 78.5% and 148%, respectively. Immobilized xylanase showed high residual activity at broad pH and temperature ranges. Using birchwood xylan as a substrate, the $V_{max}$ and $K_m$ values of xylanase nanoflowers were 1.60 mg/ml and $455{\mu}mol/min/mg$ protein, compared with 1.42 mg/ml and $300{\mu}mol/min/mg$ protein, respectively, for the free enzyme. After 5 and 10 cycles of reuse, the xylanase nanoflowers retained 87.5% and 75.8% residual activity, respectively. These results demonstrate that xylanase immobilization using a proteininorganic hybrid nanoflower system is an effective approach for its potential biotechnological applications.

• Journal of Plant Biotechnology
• /
• 제47권3호
• /
• pp.209-217
• /
• 2020

The presence of high amounts of seed storage proteins (SSPs) improves the overall quality of soybean seeds. However, these SSPs pose a major limitation due to their high abundance in soybean seeds. Although various technical advancements including mass-spectrometry and bioinformatics resources were reported, only limited information has been derived to date on soybean seeds at proteome level. Here, we applied a tandem mass tags (TMT)-based quantitative proteomic analysis to identify the significantly modulated proteins in the seeds of two soybean cultivars showing varying protein contents. This approach led to the identification of 5,678 proteins of which 13 and 1,133 proteins showed significant changes in Daewon (low-protein content cultivar) and Saedanbaek (high-protein content cultivar) respectively. Functional annotation revealed that proteins with increased abundance in Saedanbaek were mainly associated with the amino acid and protein metabolism involved in protein synthesis, folding, targeting, and degradation. Taken together, the results presented here provide a pipeline for soybean seed proteome analysis and contribute a better understanding of proteomic changes that may lead to alteration in the protein contents in soybean seeds.

• 자원환경지질
• /
• 제36권3호
• /
• pp.243-255
• /
• 2003

유용광물자원탐사나 산사태 취약성 분석과 같은 지질학적 응용을 목적으로 GIS를 이용하여 다양한 지질자료를 통합하기 위한 수학적 모델이 개발되어 왔다. 여러 공간통합 방법 중에서 불확실한 정보를 효율적으로 다룰 수 있는 것으로 알려진 퍼지 이론을 이용한 지질정보의 통합에 대해서 논의하였다. 그동안 전문가의 의견에 의존하여 지질자료를 표현하는 목표 유도형 통합방법과 달리, 통합 목표와 지질자료 사이의 통계적 관계를 이용하는 자료 유도형 통합 방법을 제안하였다. 제안된 기법은 퍼지 소속함수로의 표현, 퍼지 연산자를 이용한 결합, 비퍼지화, 검증의 4단계로 구성된다. 자료 표현에는 우도비에 기반한 퍼지 소속함수를, 퍼지 소속함수들의 결합에는 퍼지 연산자 네트웍을, 통합결과의 상대적인 가능성값을 도시하기 위해 비퍼지화 단계를 각각 제안하였다. 최종적으로 통합 목표에 대한 의미있는 해석과 다양한 퍼지 연산자 네트웍의 정량적 비교를 위해 공간 분할에 기반한 검증 과정을 제안하였다. 지질학적 응용을 목적으로 제안한 방법론의 적용가능성, 실제 적용시의 제안점을 산사태 취약성 분석 적용연구를 통해 논의하였다. 적용연구 결과, 대상지역에서 산사태에 대한 취약한 지역을 구분하는데 제안기법이 효과적으로 이용될 수 있음을 확인할 수 있었으며, 검증을 통해 최종 퍼지 소속함수의 결합에 ${\gamma}$연산자를 사용한 경우가 최대, 최소 연산자를 사용한 경우에 비해 높은 예측능력을 나타내었다.

• 대한토목학회논문집
• /
• 제8권1호
• /
• pp.113-125
• /
• 1988

우리 나라의 하천종합개발계획(河川綜合開發計劃) 수립의 기준이 되는 계획홍수량(計劃洪水量) 결정수단으로 활용되어 왔던 홍수량공식(洪水量公式) 들을 조사하였다. 이들 가운데 11종(種)의 홍수량(洪水量) 공식(公式)을 선정하여 유역규모(流域規模)에 따른 계획비홍수량(計劃比洪水量)을 계산 비교하였다. 강우조건과 유역조건이 가능한 범위까지 같은 경우가 될 수 있게 하여 검토한 바 같은 유역에서 극치들의 변동폭이 400%나 되는 편차를 가지며 이런 범위안에서 각공식 별로 서로 많은 차이를 보여 주게 됨을 알 수 있었다. 지금 쓰이고 있는 홍수량(洪水量) 산정의 절차는 무척 복잡하고 많은 노력(努力)을 거쳐서 어렵게 결정하고 있으나 성과(成果)는 사용된 수문자료(水文資料)의 기간(期間)이나 정도(精度)에 좌우되어 공학적(工學的)인 판단(判斷)의 타당성이 결여된 경우가 많았음을 경험하였다. 따라서 보다 단순하면서 합리적(合理的)인 수단의 개발을 기왕의 홍수량(洪水量) 공식(公式) 비교에서 얻을 수 있었다.

• 대한원격탐사학회:학술대회논문집
• /
• 대한원격탐사학회 1999년도 Proceedings of International Symposium on Remote Sensing
• /
• pp.158-164
• /
• 1999

This paper investigated and evaluated the NASA's Shuttle Imaging Radar-C (SIR-C) multiple frequency SAR data for differential backscattering effects of microwave from the surface geological materials overlying the skarn type mineralization. Although an integrated approach in mineral exploration is more cost effective and is well in use, there are still many technical and scientific issues to be further investigated and researched. In this study we have reprocessed several sets of previously surveyed exploration data and experimented with fuzzy logic digital fusion of the preprocessed data with respect to chosen exploration targets. Among the numerous fuzzy logic operators, which are currently available for a data driven integrated exploration strategy, we used varying combinations of fuzzy MIN, fuzzy MAX, and fuzzy SUM operators along with Gamma operator for fusion of exploration data, including the contact metamorphic zone information. The final exploration target tested was a skarn type W-Mo-F mineralization in the study area. The fuzzy logic derived mineral potential anomaly almost exactly matched the differential backscattering anomalies on the C-band and L-band SIR_C data when overlaid on each other. Although this high degree of correlation between these two data sets is remarkable, the differential backscattering anomaly over the skarn type W-Mo-F mineralization in the study area requires further investigation.

• 한국전산구조공학회논문집
• /
• 제16권3호
• /
• pp.281-290
• /
• 2003

Co Evolutionary Structural Design(CESD) Framework is presented, which can deal with the load design and structural topology design simultaneously. The load design here is the exploration algorithm that finds the critical load patterns of the given structure. In general, the load pattern is a crucial factor in determining the structural topology and being selected from the experts어 intuition and experience. However, if any of the critical load patterns would be excluded during the process of problem formation, the solution structure might show inadequate performance under the load pattern. Otherwise if some reinforcement method such as safety factor method would be utilized, the solution structure could result in inefficient conservativeness. On the other hand, the CESD has the ability of automatically finding the most critical load patterns and can help the structural solution evolve into the robust design. The CESD is made up of a load design discipline and a structural topology design discipline both of which have the fully coupled relation each other. This coupling is resolved iteratively until the resultant solution can resist against all the possible load patterns and both disciplines evolve into the solution structure with the mutual help or competition. To verify the usefulness of this approach, the 10 bar truss and the jacket type offshore structure are presented. SORA(Sequential Optimization & Reliability Assessment) is adopted in CESD as a probabilistic optimization methodology, and its usefulness in decreasing the computational cost is verified also.