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http://dx.doi.org/10.4014/jmb.1710.10037

Immobilization of Xylanase Using a Protein-Inorganic Hybrid System  

Kumar, Ashok (Department of Chemical Engineering, Konkuk University)
Patel, Sanjay K.S. (Department of Chemical Engineering, Konkuk University)
Mardan, Bharat (Department of Chemical Engineering, Konkuk University)
Pagolu, Raviteja (Department of Chemical Engineering, Konkuk University)
Lestari, Rowina (Department of Chemical Engineering, Konkuk University)
Jeong, Seong-Hoon (Department of Chemical Engineering, Konkuk University)
Kim, Taedoo (Department of Chemical Engineering, Konkuk University)
Haw, Jung Rim (Institute of SK-KU Biomaterials, Konkuk University)
Kim, Sang-Yong (Department of Food Science & Biotechnology, Shin-Ansan University)
Kim, In-Won (Department of Chemical Engineering, Konkuk University)
Lee, Jung-Kul (Department of Chemical Engineering, Konkuk University)
Publication Information
Journal of Microbiology and Biotechnology / v.28, no.4, 2018 , pp. 638-644 More about this Journal
Abstract
In this study, the immobilization of xylanase using a protein-inorganic hybrid nanoflower system was assessed to improve the enzyme properties. The synthesis of hybrid xylanase nanoflowers was very effective at $4^{\circ}C$ for 72 h, using 0.25 mg/ml protein, and efficient immobilization of xylanase was observed, with a maximum encapsulation yield and relative activity of 78.5% and 148%, respectively. Immobilized xylanase showed high residual activity at broad pH and temperature ranges. Using birchwood xylan as a substrate, the $V_{max}$ and $K_m$ values of xylanase nanoflowers were 1.60 mg/ml and $455{\mu}mol/min/mg$ protein, compared with 1.42 mg/ml and $300{\mu}mol/min/mg$ protein, respectively, for the free enzyme. After 5 and 10 cycles of reuse, the xylanase nanoflowers retained 87.5% and 75.8% residual activity, respectively. These results demonstrate that xylanase immobilization using a proteininorganic hybrid nanoflower system is an effective approach for its potential biotechnological applications.
Keywords
Immobilization; encapsulation; protein-inorganic hybrid; reusability; Thermomyces lanuginosus; xylanase;
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Times Cited By KSCI : 6  (Citation Analysis)
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