• 생명과학회지
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• 제11권4호
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• pp.311-315
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• 2001

To identify the effects embryo developmental stage and gelrite concentration on plant regeneration in seed culture of rice, mature and immature seeds of rice were cultured on the $N_{6}$ medium supplemented with2 mg/$\ell$ 2.4-D and different levels of gelrite(0.2~1.0%). The calli formed immature embryos were produced more plants than those from mature embryos. The maximum frequency of plant regeneration was achieved in the culture of the calli of immature embryos which was harvested at the 21$^{th}$ day after pollination. The plant regeneration on the medium with gelrite was more accelerate than that on the medium with agar. The highest frequency(55%) of plant regeneration was obtained from the calli transferred to the medium with 6g/$\ell$ gelrite.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2020년도 추계국제학술대회
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• pp.58-58
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• 2020

Xanthoceras sorbifolium Bunge (yellowhorn) is a woody tree in the soapberry family, Sapindaceae, native to northern China. This species has been identified as a major woody bioenergy plant for bio-diesel production because of high oil content in seed. But the flowers do not bear fruit well while the many flowers blooming. This study was performed to regenerate in vitro plantlet using adventitious shoot formation. To establish the protocol of plant regeneration, adventitious shoots formation rate in the culture of cotyledon of immature zygotic embryos was 68.6% in 1/2 MS medium with 0.5 mg l-1 BA and 3% sucrose (w/v). In the culture of cotyledons of mature zygotic embryos, induction of adventitious shoots was needed to contain high sucrose in pre-culture medium and the frequency of shoot induction was 64.4%. Multiple shoots were induced in 0.5 mg l-1 TDZ, and rooting of shoot was induced 4.0 mg l-1 IBA. Flow cytometry analysis revealed that all the regenerated plantlets were diploid.

• 식물조직배양학회지
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• 제28권4호
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• pp.205-208
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• 2001

벼의 종자배양에서 식물체 재분화 능력을 향상시키기 위하여 2 mg/L의 2,4-D와 여러 가지 농도의 copper (0~5.0mg/L)가 첨가된 MS배지에 종자배양 한 바 캘러스 생장정도는 copper 농도에 따라 다르게 나타났는데 특히, 2.5 mg/L의 copper가 첨가된 배지에서 가장 양호하였다. 그리고 캘러스 형성률과 copper 농도 간에는 유의성 있는 차이가 인정되지 않았다. 식물체 재분화 배지에 2.5 mg/L의 copper의 첨가는 Indica 품종들의 식물체 재분화 효율을 크게 향상시켰다. Copper가 첨가되지 않은 N$_{6}$배지에서 Indica 6품종의 평균재분화율은 2.4%이었으나, copper가 첨가된 배지에서 27.4%로 높게 나타났다.

• 한국육종학회지
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• 제42권4호
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• pp.351-356
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• 2010

Brachypodium distachyon is rapidly emerged in biological study and has been currently used as a model system for genetics and functional studies for crop improvement and biofuel production. Phosphinothricin (PPT) has been widely used as a selectable agent, which raises ammonium content and induces toxicity in non-transformed plant cells. However PPT selection is not much effective on Brachypodium callus consequently reducing transformation efficiency. In order to identify the efficient conditions of PPT selection, calli obtained from mature seeds of Brachypodium (PI 254867) were cultured on the callus inducing medium (CIM) or regeneration medium (ReM) containing serial dilutions of the PPT (0, 2, 5, 10, and 15 mg/l) in dark or light condition. Callus growth and ammonium content of each treatment were measured 2 weeks after the treatment. Although callus growth and ammonium content did not show much difference in CIM, slow callus growth and increased ammonium accumulation were found in ReM. No significant difference of ammonium accumulation in response to PPT was found between dark and light conditions. In order to identify major factors affecting increased ammonium accumulation, callus was cultured on the media in combined with phytohormones (2,4-D or kinetin) and carbon sources (sucrose or maltose) containing with PPT (5 mg/l). The highest ammonium content in callus was found in the kinetin and maltose media.

• Journal of Plant Biology
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• 제36권3호
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• pp.211-218
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• 1993

Plant regeneration was accomplished from protoplast culture of rice (Oryza sativa L. cv. Taebaeg). Embryogenic callus was induced from mature seed on MS medium containing 5 mM proline, 2.5 mg/L 2,4-D, 30 g/L sucrose in the dark at 28$^{\circ}C$ and used to establish embryogenic cell suspension culture. Suspension cells were subcultured every one week in N6 medium supplemented with 5 mM proline, 200 mg/L casein hydrolysate, 2.5 mg/L 2,4-D and amino acids of AA medium. Suspension cultures were composed of cells that were densely cytoplasmic, potentially embryogenic and were at least maintained for more than 6 months in liquid medium. Protoplasts were isolated from fast-growing suspension culture cells and cultured in a slightly modified KpR medium by mixed nurse culture. Isolated protoplasts began to divide within 5~7 days and thereafter, protoplast-derived calli were sequentially transferred to callus proliferating medium that soft agar MS medium contained 2 mg/L 2,4-D and produced distinct embryogenic cells. Microcolonies were then transferred to solid medium which consisted of MS medium containing 5 mg/L kinetin, 1 mg/L NAA, 1 mg/L ABA, 30 g/L sucrose and 10 g/L sorbitol under fluorescent light. Mulitple shoots of 4~5 per callus emerged and were transferred to hormone-free MS medium for root initiation. Thereafter, The plantlets were transferred to pots of soil to mature in the culture room.

• 한국초지조사료학회지
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• 제31권3호
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• pp.229-234
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• 2011

갈대의 성숙종자로부터 최적 조직배양조건을 확립하기 위하여 종자로부터 캘러스 유도 및 식물체 재분화 체계를 확립하였다. 종자로부터 캘러스 유도시 첨가되는 auxin으로는 2,4-D가 가장 효율적이었으며, 1.0 mg/L 2,4-D와 0.5 mg/L BA가 첨가된 MS 배지에서 가장 높은 빈도로 캘러스가 유도되었다. 캘러스로부터 식물체 재분화는 cytokinin류인 BA와 kinetin이 첨가되지 않은 MS 배지에서 59.6%로 높은 재분화 효율을 나타내었다. 본 연구를 통하여 확립된 성숙종자로부터의 고효율 재분화 시스템은 유전자 형질전환기술을 이용한 신품종 갈대 개발에 유용하게 이용 될 수 있을 것이다.

• Journal of Animal Science and Technology
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• 제46권2호
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• pp.243-250
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• 2004

이탈리안 라이그래스의 최적 조직배양조건을 확립하기 위하여 ‘Jeanne’ 'Florida-80', 'Metro'세 가지 품종의 성숙종자로부터 배발생 캘러스 유도 및 캘러스로부터 식물체 재분화에 미치는 배지 첨가물질의 영향을 조사하였다. 성숙종자로부터 배발생 캘러스 유도시에 첨가되는 식물 생장조절물질로는 모든 품종에서 2,4-D의 경우 5mg/L의 농도로 첨가된 MS 배지에서 배발생 캘러스가 가장 높은 빈도로 유도되었으며, dicamba의 경우 7mg/L의 농도로 첨가해주었을 때 배발생 캘러스의 유도율이 가장 높았다. 배발생 캘러스로부터 식물체 재분화는 배발생 캘러스를 1mg/L 2,4-D와 5mg/L BA가 첨가된 N6 배지에서 배양했을 때 가장 높은 식물체 재분화율을 나타내었다. 광조건에 따른 배양효율의 차이는 모든 품종에서 배발생 캘러스의 유도시에 암상태에서 배양한 캘러스로부터 식물체 재분화율이 광상태에서 배양한 캘러스의 재분화율 보다 약10%정도 높게 나타났다. Casein hydrolyaste와 L-proline의 경우 동시에 배지에 혼용 첨가해 주었을 때 배발생 캘러스 유도율과 식물체 재분화율이 증가되었다. 본 연구를 통하여 확립된 효율적인 배발생 캘러스의 유도 및 식물체 재분화체계는 분자육종을 통한 신품종 이탈리안 라이그래스의 개발에 유용하게 이용되어질 수 있을 것이다.

• Journal of Plant Biotechnology
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• 제6권4호
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• pp.213-219
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• 2004

Zygotic embryos were excised from immature and mature seeds of the Himalayan yew, Taxus wallichiana. The embryos germinated precociously when kept in darkness for 5 weeks and developed into full seedlings within 10-12 weeks. The highest rate of embryo germination ($81\%$) was obtained in modified Lloyd & McCown' s woody plant medium containing macro and micronutrients at half strength supplemented with $1\%$ activated charcoal, which supported both the best embryonic growth ($43\%$) and seedling development ($32\%$). However, the supplementation of basal media with kinetin, thidiazuron, 6-benzyl aminopurine or $GA_3$ had no effect on the germination of the embryos. The embryos derived from immature seeds germinated but the frequency of embryonic growth was better in mature seeds. Stratification of seeds effected precocious germination of embryos. Seeds kept at $4^{\circ}C$ for 1 week germinated earlier and at a higher frequency irrespective of the stage of seed maturity, while the germination rate declined with prolonged cold treatment for 1 month at that same temperature. Analysis of taxanes in germinating seedlings revealed that root tissues contained high levels of taxol, 10-deacetyl-baccatin ill and baccatin ill as compared to shoots. Thus embryo culture technique appears to overcome the lengthy dormancy requirement of T. wallichiana seeds.

• 한국자원식물학회지
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• 제20권6호
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• pp.518-523
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• 2007

Lunasin is small subunit peptide of coded from Gm2S-1 gene in soybean. It has been previously demonstrated that lunasin is a novel and promising cancer preventive peptide. Lunasin peptide is found only in the seed and not other tissues. And lunasin peptide starts to appear at 5 weeks after flowering and remains in the mature seed. We report here firstly lunasin peptide identified from soybean callus induced by the tissue culture and demonstrate its anticancer properties. The lunasin was identified and purified from soybean callus aged for 6 months. The callus lunasin($1{\mu}M$) inhibited the acetylation of histone H3 and H4 by 58.8% and 56.5%, respectively. And it fully inhibited foci formation compared to the values of the positive control(no lunasin) and negative control(no MCA). Purified lunasin was able to internalize into the cell and localized in the nucleus.

• 한국작물학회지
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• 제42권6호
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• pp.647-651
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• 1997

무피복 재배조건에서 생육특성 및 수량성 그리고 종실의 단백질과 지방함량, 지방산 조성을 조사하여 무피복 재배법 확립을 위한 기초자료를 얻고자 대광땅콩, 대풍땅콩을 공시하고 흑색 비닐 피복재배와 비교하여 시험한 결과는 다음과 같다. 1. 출현기는 대광땅콩, 대풍땅콩 모두 피복재배보다 6일정도 지연되었으며 개화기는 각각 9일, 6일 지연되었고 입모율도 각각 3.7％, 16％감소되었다. 2. 분지장은 피복재배보다 대광땅콩 24％(11.2cm), 대풍땅콩 17％(6.9cm) 감소되었으나 분지수는 감소되지 않았다. 3. 성숙협수,100립중, 협실비율, 주당 종실수량등은 피복재배보다 현저히 감소되었으나 밀식재배로 인한 재식밀도의 증가로 1ha당 종실수량은 대광땅콩의 경우 피복재배와 비슷하였고 대풍땅콩의 경우는 26％ 증수되었다. 4. 단백질함량은 대광땅콩의 경우, 피복재배보다 4％ 증가하였으나 대풍땅콩은 3％ 감소하였으며 지방함량은 두 품종 모두 각각 7％, 9％ 감소되었다. 5. 지방산 조성에서 oleic acid는 대광, 대풍땅콩 모두 피복재배보다 증가하였으나 linolelc acid는 감소하였으며 O/L비율도 작아졌다.